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The map label for this gene is capD [H]

Identifier: 82701764

GI number: 82701764

Start: 717475

End: 719487

Strand: Reverse

Name: capD [H]

Synonym: Nmul_A0631

Alternate gene names: 82701764

Gene position: 719487-717475 (Counterclockwise)

Preceding gene: 82701765

Following gene: 82701760

Centisome position: 22.6

GC content: 55.54

Gene sequence:

>2013_bases
ATGCCCGCGCCCAAACTCAACATAAGGACGGTTATTGCATTCGGGCATGATGTTGTAGCTGCCGCCATCGCCTGGTCCCT
GGCTTATCTGTTCCGCTTCAATTTCGAGATACCCTTCGTTTATCTGGCATCTTTGGAAGAGATTCTGCCGTGGGTGGTCC
CGATACACGCAGCAGCCTTCTTATTCTTTGGCCTGTATCGTGGACTCTGGCATTACGCAAGCCTGCCCGATTTGCGGCGC
ATCCTGTTTGCTGTATCCGCTTCGGCTGCAGCCGTCCCATTGGTGCTATACATGTTGCAGATACTGGTCGGTGTCCCCAG
AACTGTGCTGATACTGGCCCCCATCCTGCTGCTGTTCATCATGGGAAGCAGCCGTCTCGCTTACCGGTTCTGGAAAGAAC
ACCGGCTTTACGGCCGCAGTAAAACGGGAGGCAATCTCGTTCTGGTGATAGGAGCAAGTGATGCTGCTGTCGGCTTGGTA
AAAGAGCTGGCGCGCAATGTGGAATGGCGCGTCGCGGGTTTCCTTGACGACGATCCGGCCAAGCGGGGGCTGATGCTGCA
CGGATTCAAAGTACTGGGCCGCATCAACGATCTGCCGGAGGTAGCCCGGAAACTGGGTGTCGCGCATGCCATTATCGCTC
TCACCTCCTCCGCTTCAGATCGCCGCAAATCCTATCGCACCCACTCTGATCGCCGCCGCCCCGACCGCCTCCTGCGCGAC
CGACGTCGAGCCTTGCAACTGTGTGCAGCGGCAGGGGTAAAGGCCTTGATCGTCCCTTCCTATAACGACCTGGTGAGCGG
CAACATCAAGGTGTCACAAATCCGGACAGTCGAGCCTGAAGATTTGCTGGGGCGGGATCCTGTCGTGCTGGATAACGATG
GACTGCACGATTTACTGACGGGAAAAACGGTATTGGTTACAGGCGCGGGGGGTTCGATTGGATCGGAGTTATGCCGACAG
ATCGTCAAATTCGCACCGGCTCAACTGGTACTGTTTGAATTGAATGAATTTGCGCTGTACAGCATAGAGCAGGAATTCCA
GGCCGATTTTCCAGAGATTCCCATGATATTCGCCATTGGCGATGTCAAGGATGAAGCGCGGCTATCACAGGTATTCCTGC
AATACCGGCCGGCTATTGTGTTTCATGCGGCTGCCTACAAGCATGTGCCCCTGATGGAACAGGAAAATGCATGGCAGGCG
GTGCTGAACAACGTGCGGGGAACCTATGTCCTGGCGCAAACCGCCATCCGGTACGGGGTGGAGAAATTCGTCCTGATTTC
GACCGACAAGGCCGTCAACCCGACCAATGTCATGGGCGCGAGTAAACGCCTGGCTGAAATGGTATGCCAGGCGTTACAGC
AGTCGATTGCATCTCCTGACAGTCCCACCGAAAACGCCTCCGGCAAAATTGTTCTGGGCAGCAGGCTTGAAAGGAGGCAG
GAGCCACGATTCGTCATGGTGCGTTTCGGTAATGTGCTGGGCAGTGCGGGCAGCGTGATTCCCAAATTCAGGGAGCAGAT
TGAAAAAGGGGGACCGATAACGGTAACGCACTCGGAAATCACCCGTTATTTCATGTCCATACCTGAGGCAGCACAGTTGG
TTCTACAGGCCGGCTTGATGGGCGGCAAGCGGCGGGGGGGGGAAATTTTCGTGCTGGATATGGGCGAACCGATTAAAATC
GCCGATCTTGCGCGAGATCTGATTCGTCTTTCCGGATTGAGCGAGGATGAAATAAAAATTGTTTACAATGGCTTGCGCCC
CGGCGAAAAACTCTACGAAGAACTGTTGGCGGATGACGAAAATACGCTTCCCACGCCCCACCCCAAATTGCGTATCGCGC
AAGCACGTCAGGTAGACAGAAAATGGCTGACAGCTCTGCTGGCGTGGCTTGAGGAGCATCCCGCCCTGAGCGACGAAGAG
GTCAAGCGGGAGCTGCCCAGATGGGTTCCGGAATACGTGAGCGCGGAATCAGTTATCCCCGTTTCCCAGGTTCGCGAGTC
TCGTATCGCATAG

Upstream 100 bases:

>100_bases
AACGTTCAGTTCGGAATGGTGACCGCGTGGATCGGAATTTATCTGGTCATGATATGCCTTTGCGATCGCCGTCTGCGGCA
TGCCCCGGATAGTCCATAGC

Downstream 100 bases:

>100_bases
GCGCTGCTTCTTGTTGCATTGCCGTTGCAGCTTTTGACCCAAATGCTGAATGGTGGTTGGAATGCTTACGATTGCGCCAT
TTGATAGCGCGAGCCCCTAA

Product: polysaccharide biosynthesis protein CapD

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 670; Mature: 669

Protein sequence:

>670_residues
MPAPKLNIRTVIAFGHDVVAAAIAWSLAYLFRFNFEIPFVYLASLEEILPWVVPIHAAAFLFFGLYRGLWHYASLPDLRR
ILFAVSASAAAVPLVLYMLQILVGVPRTVLILAPILLLFIMGSSRLAYRFWKEHRLYGRSKTGGNLVLVIGASDAAVGLV
KELARNVEWRVAGFLDDDPAKRGLMLHGFKVLGRINDLPEVARKLGVAHAIIALTSSASDRRKSYRTHSDRRRPDRLLRD
RRRALQLCAAAGVKALIVPSYNDLVSGNIKVSQIRTVEPEDLLGRDPVVLDNDGLHDLLTGKTVLVTGAGGSIGSELCRQ
IVKFAPAQLVLFELNEFALYSIEQEFQADFPEIPMIFAIGDVKDEARLSQVFLQYRPAIVFHAAAYKHVPLMEQENAWQA
VLNNVRGTYVLAQTAIRYGVEKFVLISTDKAVNPTNVMGASKRLAEMVCQALQQSIASPDSPTENASGKIVLGSRLERRQ
EPRFVMVRFGNVLGSAGSVIPKFREQIEKGGPITVTHSEITRYFMSIPEAAQLVLQAGLMGGKRRGGEIFVLDMGEPIKI
ADLARDLIRLSGLSEDEIKIVYNGLRPGEKLYEELLADDENTLPTPHPKLRIAQARQVDRKWLTALLAWLEEHPALSDEE
VKRELPRWVPEYVSAESVIPVSQVRESRIA

Sequences:

>Translated_670_residues
MPAPKLNIRTVIAFGHDVVAAAIAWSLAYLFRFNFEIPFVYLASLEEILPWVVPIHAAAFLFFGLYRGLWHYASLPDLRR
ILFAVSASAAAVPLVLYMLQILVGVPRTVLILAPILLLFIMGSSRLAYRFWKEHRLYGRSKTGGNLVLVIGASDAAVGLV
KELARNVEWRVAGFLDDDPAKRGLMLHGFKVLGRINDLPEVARKLGVAHAIIALTSSASDRRKSYRTHSDRRRPDRLLRD
RRRALQLCAAAGVKALIVPSYNDLVSGNIKVSQIRTVEPEDLLGRDPVVLDNDGLHDLLTGKTVLVTGAGGSIGSELCRQ
IVKFAPAQLVLFELNEFALYSIEQEFQADFPEIPMIFAIGDVKDEARLSQVFLQYRPAIVFHAAAYKHVPLMEQENAWQA
VLNNVRGTYVLAQTAIRYGVEKFVLISTDKAVNPTNVMGASKRLAEMVCQALQQSIASPDSPTENASGKIVLGSRLERRQ
EPRFVMVRFGNVLGSAGSVIPKFREQIEKGGPITVTHSEITRYFMSIPEAAQLVLQAGLMGGKRRGGEIFVLDMGEPIKI
ADLARDLIRLSGLSEDEIKIVYNGLRPGEKLYEELLADDENTLPTPHPKLRIAQARQVDRKWLTALLAWLEEHPALSDEE
VKRELPRWVPEYVSAESVIPVSQVRESRIA
>Mature_669_residues
PAPKLNIRTVIAFGHDVVAAAIAWSLAYLFRFNFEIPFVYLASLEEILPWVVPIHAAAFLFFGLYRGLWHYASLPDLRRI
LFAVSASAAAVPLVLYMLQILVGVPRTVLILAPILLLFIMGSSRLAYRFWKEHRLYGRSKTGGNLVLVIGASDAAVGLVK
ELARNVEWRVAGFLDDDPAKRGLMLHGFKVLGRINDLPEVARKLGVAHAIIALTSSASDRRKSYRTHSDRRRPDRLLRDR
RRALQLCAAAGVKALIVPSYNDLVSGNIKVSQIRTVEPEDLLGRDPVVLDNDGLHDLLTGKTVLVTGAGGSIGSELCRQI
VKFAPAQLVLFELNEFALYSIEQEFQADFPEIPMIFAIGDVKDEARLSQVFLQYRPAIVFHAAAYKHVPLMEQENAWQAV
LNNVRGTYVLAQTAIRYGVEKFVLISTDKAVNPTNVMGASKRLAEMVCQALQQSIASPDSPTENASGKIVLGSRLERRQE
PRFVMVRFGNVLGSAGSVIPKFREQIEKGGPITVTHSEITRYFMSIPEAAQLVLQAGLMGGKRRGGEIFVLDMGEPIKIA
DLARDLIRLSGLSEDEIKIVYNGLRPGEKLYEELLADDENTLPTPHPKLRIAQARQVDRKWLTALLAWLEEHPALSDEEV
KRELPRWVPEYVSAESVIPVSQVRESRIA

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 74495; Mature: 74363

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPKLNIRTVIAFGHDVVAAAIAWSLAYLFRFNFEIPFVYLASLEEILPWVVPIHAAAF
CCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LFFGLYRGLWHYASLPDLRRILFAVSASAAAVPLVLYMLQILVGVPRTVLILAPILLLFI
HHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
MGSSRLAYRFWKEHRLYGRSKTGGNLVLVIGASDAAVGLVKELARNVEWRVAGFLDDDPA
HCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCHH
KRGLMLHGFKVLGRINDLPEVARKLGVAHAIIALTSSASDRRKSYRTHSDRRRPDRLLRD
HCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHH
RRRALQLCAAAGVKALIVPSYNDLVSGNIKVSQIRTVEPEDLLGRDPVVLDNDGLHDLLT
HHHHHHHHHHCCCCEEEECCCHHHHCCCEEEEEEEECCHHHHCCCCCEEECCCCCHHHHC
GKTVLVTGAGGSIGSELCRQIVKFAPAQLVLFELNEFALYSIEQEFQADFPEIPMIFAIG
CCEEEEECCCCCHHHHHHHHHHHHCCHHEEEEECCHHHHHHHHHHHCCCCCCCCEEEEEC
DVKDEARLSQVFLQYRPAIVFHAAAYKHVPLMEQENAWQAVLNNVRGTYVLAQTAIRYGV
CCCHHHHHHHHHHHHCCHHEEEHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCC
EKFVLISTDKAVNPTNVMGASKRLAEMVCQALQQSIASPDSPTENASGKIVLGSRLERRQ
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHCC
EPRFVMVRFGNVLGSAGSVIPKFREQIEKGGPITVTHSEITRYFMSIPEAAQLVLQAGLM
CCCEEEEEEHHHHCCCCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHCCHHHHHHHHHHHC
GGKRRGGEIFVLDMGEPIKIADLARDLIRLSGLSEDEIKIVYNGLRPGEKLYEELLADDE
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCC
NTLPTPHPKLRIAQARQVDRKWLTALLAWLEEHPALSDEEVKRELPRWVPEYVSAESVIP
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCC
VSQVRESRIA
HHHHHHHCCC
>Mature Secondary Structure 
PAPKLNIRTVIAFGHDVVAAAIAWSLAYLFRFNFEIPFVYLASLEEILPWVVPIHAAAF
CCCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LFFGLYRGLWHYASLPDLRRILFAVSASAAAVPLVLYMLQILVGVPRTVLILAPILLLFI
HHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
MGSSRLAYRFWKEHRLYGRSKTGGNLVLVIGASDAAVGLVKELARNVEWRVAGFLDDDPA
HCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCHH
KRGLMLHGFKVLGRINDLPEVARKLGVAHAIIALTSSASDRRKSYRTHSDRRRPDRLLRD
HCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHH
RRRALQLCAAAGVKALIVPSYNDLVSGNIKVSQIRTVEPEDLLGRDPVVLDNDGLHDLLT
HHHHHHHHHHCCCCEEEECCCHHHHCCCEEEEEEEECCHHHHCCCCCEEECCCCCHHHHC
GKTVLVTGAGGSIGSELCRQIVKFAPAQLVLFELNEFALYSIEQEFQADFPEIPMIFAIG
CCEEEEECCCCCHHHHHHHHHHHHCCHHEEEEECCHHHHHHHHHHHCCCCCCCCEEEEEC
DVKDEARLSQVFLQYRPAIVFHAAAYKHVPLMEQENAWQAVLNNVRGTYVLAQTAIRYGV
CCCHHHHHHHHHHHHCCHHEEEHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCC
EKFVLISTDKAVNPTNVMGASKRLAEMVCQALQQSIASPDSPTENASGKIVLGSRLERRQ
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHCC
EPRFVMVRFGNVLGSAGSVIPKFREQIEKGGPITVTHSEITRYFMSIPEAAQLVLQAGLM
CCCEEEEEEHHHHCCCCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHCCHHHHHHHHHHHC
GGKRRGGEIFVLDMGEPIKIADLARDLIRLSGLSEDEIKIVYNGLRPGEKLYEELLADDE
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCC
NTLPTPHPKLRIAQARQVDRKWLTALLAWLEEHPALSDEEVKRELPRWVPEYVSAESVIP
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCC
VSQVRESRIA
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]