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The map label for this gene is prs [H]

Identifier: 82701723

GI number: 82701723

Start: 673818

End: 674768

Strand: Direct

Name: prs [H]

Synonym: Nmul_A0589

Alternate gene names: 82701723

Gene position: 673818-674768 (Clockwise)

Preceding gene: 82701722

Following gene: 82701724

Centisome position: 21.16

GC content: 58.25

Gene sequence:

>951_bases
ATGGCCTACGATAGCTTGATGGTTTTCACGGGCAACGCCAATCCCAAGCTGGCGCAGGATGTCGTACGGCATCTCAATCT
TCGCATCGGCCGTGCCACGGTTGGCCGCTTCAGCGATGGCGAAGTAATGGTGGAAATCCTCGAGAATGTCCGGGGCAAGG
ATGTATTCGTCCTGCAATCCACCTGCATGCCCACCAACGATACGCTGATGGAATTACTGGTGCTGGTGGATGCGCTCAAG
CGCGCCTCCGCGTCGCGCATCACCGCCGCGATGCCTTATTTCGGCTATGCCCGCCAGGACCGGCGCCCGCGCTCGGTACG
GGTCCCCATCACGGCCAAGGTGGTGGCAAACATGCTGACCAGTGTCGGCATAGACCGCTTGCTGACAATGGACTTGCATT
CGGACCAGATCCAGGGGTTTTTTGACATACCTGTGGATAACATCTACAGCATGCCTATCCTGCTGGGCGATCTCTGGAAA
AATGATTTCAAGAACCTGGTGGTGGTTTCCCCTGATGTGGGGGGAGTAGTGCGCGCGCGCCAGATGGCCAAACGGCTCGA
ATGCGACCTTGCCATCATAGACAAGCGGCGTCCCAAAGCGAATATAGCAAAGGTGATGAACATCATCGGCGAGGTGGACG
GACGCACTTGTGTGATCATGGACGACATGGTGGATACCGCTAATACGCTGTGCGAAGCAGCGCGCGCGCTCAAGGAGCAG
GGAGCGGAACGCGTGCTGGCTTATTGCACGCATCCGGTGCTGTCGGGCGGTGCCGTGGAGCGGATCGGGAACTCTGCCCT
GGACAAGCTCGTTGTCACCGACACCATTCCGCTGCGGGAGGATGCCCAGGCGTGCGAGCGCATAGAGCAGTTGAGCGTGG
CAAGCCTGCTCGCGGAAACGATGCTCAGGATCAGTAACGAAGATTCCGTGAGTTCGCTTTTCATGGAGTAA

Upstream 100 bases:

>100_bases
AGCTCCATGATTCGTCCAGGCTGGACTGCCGCCGGTTCCTGTTCGCTTCCATGAATTCCCCATCTGCCGGTTCCGCGTTT
TCGGACTTTTAGGTTTTCTC

Downstream 100 bases:

>100_bases
CGCGAATCCACGCTGTGCAGGTGGTGAAAAGCAAAACCAGGGAGCAAGGCTGGTTGAGAATAGCCTGCGGATTGAATAGA
TAGCGCAATAGACAAGTTTA

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MAYDSLMVFTGNANPKLAQDVVRHLNLRIGRATVGRFSDGEVMVEILENVRGKDVFVLQSTCMPTNDTLMELLVLVDALK
RASASRITAAMPYFGYARQDRRPRSVRVPITAKVVANMLTSVGIDRLLTMDLHSDQIQGFFDIPVDNIYSMPILLGDLWK
NDFKNLVVVSPDVGGVVRARQMAKRLECDLAIIDKRRPKANIAKVMNIIGEVDGRTCVIMDDMVDTANTLCEAARALKEQ
GAERVLAYCTHPVLSGGAVERIGNSALDKLVVTDTIPLREDAQACERIEQLSVASLLAETMLRISNEDSVSSLFME

Sequences:

>Translated_316_residues
MAYDSLMVFTGNANPKLAQDVVRHLNLRIGRATVGRFSDGEVMVEILENVRGKDVFVLQSTCMPTNDTLMELLVLVDALK
RASASRITAAMPYFGYARQDRRPRSVRVPITAKVVANMLTSVGIDRLLTMDLHSDQIQGFFDIPVDNIYSMPILLGDLWK
NDFKNLVVVSPDVGGVVRARQMAKRLECDLAIIDKRRPKANIAKVMNIIGEVDGRTCVIMDDMVDTANTLCEAARALKEQ
GAERVLAYCTHPVLSGGAVERIGNSALDKLVVTDTIPLREDAQACERIEQLSVASLLAETMLRISNEDSVSSLFME
>Mature_315_residues
AYDSLMVFTGNANPKLAQDVVRHLNLRIGRATVGRFSDGEVMVEILENVRGKDVFVLQSTCMPTNDTLMELLVLVDALKR
ASASRITAAMPYFGYARQDRRPRSVRVPITAKVVANMLTSVGIDRLLTMDLHSDQIQGFFDIPVDNIYSMPILLGDLWKN
DFKNLVVVSPDVGGVVRARQMAKRLECDLAIIDKRRPKANIAKVMNIIGEVDGRTCVIMDDMVDTANTLCEAARALKEQG
AERVLAYCTHPVLSGGAVERIGNSALDKLVVTDTIPLREDAQACERIEQLSVASLLAETMLRISNEDSVSSLFME

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=312, Percent_Identity=45.8333333333333, Blast_Score=293, Evalue=1e-79,
Organism=Homo sapiens, GI4506129, Length=312, Percent_Identity=45.8333333333333, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI84875539, Length=314, Percent_Identity=45.2229299363057, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI28557709, Length=312, Percent_Identity=45.5128205128205, Blast_Score=288, Evalue=4e-78,
Organism=Homo sapiens, GI4506133, Length=345, Percent_Identity=33.9130434782609, Blast_Score=191, Evalue=9e-49,
Organism=Homo sapiens, GI194018537, Length=342, Percent_Identity=33.3333333333333, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI310128524, Length=151, Percent_Identity=33.7748344370861, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI310115209, Length=151, Percent_Identity=33.7748344370861, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI310118259, Length=151, Percent_Identity=33.7748344370861, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI310119946, Length=151, Percent_Identity=33.7748344370861, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI1787458, Length=310, Percent_Identity=60.9677419354839, Blast_Score=403, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17554704, Length=312, Percent_Identity=44.2307692307692, Blast_Score=288, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17554702, Length=312, Percent_Identity=43.9102564102564, Blast_Score=288, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI25149168, Length=312, Percent_Identity=43.9102564102564, Blast_Score=288, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI71989924, Length=312, Percent_Identity=43.9102564102564, Blast_Score=287, Evalue=5e-78,
Organism=Caenorhabditis elegans, GI17570245, Length=342, Percent_Identity=33.3333333333333, Blast_Score=196, Evalue=9e-51,
Organism=Saccharomyces cerevisiae, GI6321776, Length=316, Percent_Identity=43.0379746835443, Blast_Score=264, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6320946, Length=319, Percent_Identity=42.0062695924765, Blast_Score=261, Evalue=9e-71,
Organism=Saccharomyces cerevisiae, GI6319403, Length=318, Percent_Identity=42.1383647798742, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6322667, Length=196, Percent_Identity=41.3265306122449, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6324511, Length=118, Percent_Identity=40.6779661016949, Blast_Score=104, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21355239, Length=312, Percent_Identity=45.8333333333333, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI45551540, Length=334, Percent_Identity=42.5149700598802, Blast_Score=272, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24651458, Length=354, Percent_Identity=31.9209039548023, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24651456, Length=354, Percent_Identity=31.9209039548023, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI281362873, Length=354, Percent_Identity=31.9209039548023, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24651454, Length=354, Percent_Identity=31.9209039548023, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24651462, Length=212, Percent_Identity=35.377358490566, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24651464, Length=212, Percent_Identity=35.377358490566, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI45552010, Length=212, Percent_Identity=35.377358490566, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 34872; Mature: 34741

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYDSLMVFTGNANPKLAQDVVRHLNLRIGRATVGRFSDGEVMVEILENVRGKDVFVLQS
CCCCCEEEEECCCCCHHHHHHHHHHCCEECCCCCCCCCCCHHHHHHHHCCCCCEEEEEEC
TCMPTNDTLMELLVLVDALKRASASRITAAMPYFGYARQDRRPRSVRVPITAKVVANMLT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCEEECCHHHHHHHHHHH
SVGIDRLLTMDLHSDQIQGFFDIPVDNIYSMPILLGDLWKNDFKNLVVVSPDVGGVVRAR
HHCHHHHEEECCCHHHCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHH
QMAKRLECDLAIIDKRRPKANIAKVMNIIGEVDGRTCVIMDDMVDTANTLCEAARALKEQ
HHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
GAERVLAYCTHPVLSGGAVERIGNSALDKLVVTDTIPLREDAQACERIEQLSVASLLAET
HHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHH
MLRISNEDSVSSLFME
HHHHCCCCHHHHHHCC
>Mature Secondary Structure 
AYDSLMVFTGNANPKLAQDVVRHLNLRIGRATVGRFSDGEVMVEILENVRGKDVFVLQS
CCCCEEEEECCCCCHHHHHHHHHHCCEECCCCCCCCCCCHHHHHHHHCCCCCEEEEEEC
TCMPTNDTLMELLVLVDALKRASASRITAAMPYFGYARQDRRPRSVRVPITAKVVANMLT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCEEECCHHHHHHHHHHH
SVGIDRLLTMDLHSDQIQGFFDIPVDNIYSMPILLGDLWKNDFKNLVVVSPDVGGVVRAR
HHCHHHHEEECCCHHHCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHH
QMAKRLECDLAIIDKRRPKANIAKVMNIIGEVDGRTCVIMDDMVDTANTLCEAARALKEQ
HHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
GAERVLAYCTHPVLSGGAVERIGNSALDKLVVTDTIPLREDAQACERIEQLSVASLLAET
HHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHH
MLRISNEDSVSSLFME
HHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]