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The map label for this gene is ispE

Identifier: 82701722

GI number: 82701722

Start: 672751

End: 673635

Strand: Direct

Name: ispE

Synonym: Nmul_A0588

Alternate gene names: 82701722

Gene position: 672751-673635 (Clockwise)

Preceding gene: 82701721

Following gene: 82701723

Centisome position: 21.13

GC content: 56.84

Gene sequence:

>885_bases
ATGAATGGAATACTGTCAACGACTGAAGTGGATTCGCAGGCGGAACTGAGCTGCCCTGCGCCTGCCAAACTCAATCTGTT
CCTGCATGTGGTGGGACGGAGGGAGGATGGGTACCATCTTCTGCAAACCGTTTTCCGCCTGGTGGATTTCGCCGACCAGC
TCCATTTCGGGCTGCGGGCGGACGGTGTGATCAAGCTGCATACGCCCACTCCGGGGGTGCCGGAAGAGCAGGATTTGTGC
GTGCGCGCAGCAAAACTGCTGCAACGGGAAAGCGGTACTCCCTGGGGGGCCAATATCTTTCTGGAAAAGCGCATCCCGAT
GGGTGGTGGCCTGGGAGGCGGCAGTTCGGATGCAGCCACGACATTGCTTGCGCTCAACCGCTTGTGGAAGCTGGGCTGGC
GCCGGAATCAACTTTTGAAACTGGCCCCGGAACTGGGTGCGGATGTTCCCGTATTCGTTTTCAGTGAAAATGCCTTTGCT
GAGGGCATCGGCGAAAAACTCCTGCCGATTGCGTTACCCCCGGCATGGTATCTGATACTCACACCGCCCGTGCATGTCTC
AACGGCAAAGGTTTTTTCAAGTAAGGAATTGACACGAAACACGATTCCGATCAAAATACCGCCCTTTTCCACCGAGCAAG
GGCATAATGATCTCGAGCCGGTGGTGTGTGCTTCATACCCCGAGGTAGCACGCCACCTCGAGTGGCTGCGGCAGCTCGAA
GGTGCAAGGATGGCGGCCATGACGGGTTCCGGCGCGTGCGTTTTTGCCGAGTTTGCGACCGAATCCGGGGCCAGAAGCGC
ACTGGGGAAGATTCCATACGGTATGAAGGGTTTTGTGGCGCAGGGACTTGATCGCCATCCCTTGCATGATTTTGCAGAAC
AATAA

Upstream 100 bases:

>100_bases
TACTCGCAGGCGGTACCTGCTGAAGCTCCACAGTCAGAGGCCGCGCGTGCCAGACTGCTGACACTTAACCGTAACGGTCT
GCGGATAAGGCTGGTAATAG

Downstream 100 bases:

>100_bases
TTGGGGAGTCGCCAAGTGGTAAGGCACCGGATTTTGATTCCGGCATTCGTAGGTTCGATCCCTACCTCCCCAGCCAGTTT
TCAGCTCCATGATTCGTCCA

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MNGILSTTEVDSQAELSCPAPAKLNLFLHVVGRREDGYHLLQTVFRLVDFADQLHFGLRADGVIKLHTPTPGVPEEQDLC
VRAAKLLQRESGTPWGANIFLEKRIPMGGGLGGGSSDAATTLLALNRLWKLGWRRNQLLKLAPELGADVPVFVFSENAFA
EGIGEKLLPIALPPAWYLILTPPVHVSTAKVFSSKELTRNTIPIKIPPFSTEQGHNDLEPVVCASYPEVARHLEWLRQLE
GARMAAMTGSGACVFAEFATESGARSALGKIPYGMKGFVAQGLDRHPLHDFAEQ

Sequences:

>Translated_294_residues
MNGILSTTEVDSQAELSCPAPAKLNLFLHVVGRREDGYHLLQTVFRLVDFADQLHFGLRADGVIKLHTPTPGVPEEQDLC
VRAAKLLQRESGTPWGANIFLEKRIPMGGGLGGGSSDAATTLLALNRLWKLGWRRNQLLKLAPELGADVPVFVFSENAFA
EGIGEKLLPIALPPAWYLILTPPVHVSTAKVFSSKELTRNTIPIKIPPFSTEQGHNDLEPVVCASYPEVARHLEWLRQLE
GARMAAMTGSGACVFAEFATESGARSALGKIPYGMKGFVAQGLDRHPLHDFAEQ
>Mature_294_residues
MNGILSTTEVDSQAELSCPAPAKLNLFLHVVGRREDGYHLLQTVFRLVDFADQLHFGLRADGVIKLHTPTPGVPEEQDLC
VRAAKLLQRESGTPWGANIFLEKRIPMGGGLGGGSSDAATTLLALNRLWKLGWRRNQLLKLAPELGADVPVFVFSENAFA
EGIGEKLLPIALPPAWYLILTPPVHVSTAKVFSSKELTRNTIPIKIPPFSTEQGHNDLEPVVCASYPEVARHLEWLRQLE
GARMAAMTGSGACVFAEFATESGARSALGKIPYGMKGFVAQGLDRHPLHDFAEQ

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=277, Percent_Identity=49.0974729241877, Blast_Score=230, Evalue=7e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_NITMU (Q2YBH5)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411288.1
- ProteinModelPortal:   Q2YBH5
- SMR:   Q2YBH5
- STRING:   Q2YBH5
- GeneID:   3783986
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0588
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   LNLFLHV
- PhylomeDB:   Q2YBH5
- BioCyc:   NMUL323848:NMUL_A0588-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 32008; Mature: 32008

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: ACT_SITE 23-23 ACT_SITE 148-148

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGILSTTEVDSQAELSCPAPAKLNLFLHVVGRREDGYHLLQTVFRLVDFADQLHFGLRA
CCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC
DGVIKLHTPTPGVPEEQDLCVRAAKLLQRESGTPWGANIFLEKRIPMGGGLGGGSSDAAT
CCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHH
TLLALNRLWKLGWRRNQLLKLAPELGADVPVFVFSENAFAEGIGEKLLPIALPPAWYLIL
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHHCCCCCEEECCCCEEEEE
TPPVHVSTAKVFSSKELTRNTIPIKIPPFSTEQGHNDLEPVVCASYPEVARHLEWLRQLE
CCCCCCHHHHHHCCHHHHCCCCCEEECCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHC
GARMAAMTGSGACVFAEFATESGARSALGKIPYGMKGFVAQGLDRHPLHDFAEQ
CCEEEEECCCCCEEEEEHHHCCCHHHHHHCCCCCCHHHHHCCCCCCCCHHHHCC
>Mature Secondary Structure
MNGILSTTEVDSQAELSCPAPAKLNLFLHVVGRREDGYHLLQTVFRLVDFADQLHFGLRA
CCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC
DGVIKLHTPTPGVPEEQDLCVRAAKLLQRESGTPWGANIFLEKRIPMGGGLGGGSSDAAT
CCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHH
TLLALNRLWKLGWRRNQLLKLAPELGADVPVFVFSENAFAEGIGEKLLPIALPPAWYLIL
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHHCCCCCEEECCCCEEEEE
TPPVHVSTAKVFSSKELTRNTIPIKIPPFSTEQGHNDLEPVVCASYPEVARHLEWLRQLE
CCCCCCHHHHHHCCHHHHCCCCCEEECCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHC
GARMAAMTGSGACVFAEFATESGARSALGKIPYGMKGFVAQGLDRHPLHDFAEQ
CCEEEEECCCCCEEEEEHHHCCCHHHHHHCCCCCCHHHHHCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA