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The map label for this gene is uvrD [H]

Identifier: 82701697

GI number: 82701697

Start: 648673

End: 650904

Strand: Direct

Name: uvrD [H]

Synonym: Nmul_A0563

Alternate gene names: 82701697

Gene position: 648673-650904 (Clockwise)

Preceding gene: 82701696

Following gene: 82701702

Centisome position: 20.37

GC content: 56.72

Gene sequence:

>2232_bases
ATGTCTTCTCTACTCACCGCACTAAACCCGGAACAACTCGAAGCAGTTACGTTGCCCCATCAGTCGGCGTTGGTCCTTGC
CGGCGCGGGCAGCGGCAAGACCAAGGTCCTTACGACCCGCATCGCGTATCTGATCCAGTCCGGCGAAGTCAGTCCTCACG
GAATATTGGCCGTCACTTTTACCAATAAGGCGGCCAAGGAAATGCTTACTCGCATTGCCGCCATGCTGCCCATCAATACG
CGCGGCATGTGGATAGGTACCTTCCACGGCTTATGCAACCGCCTGCTTCGCGCTCATCACCAGGATGCCGGTTTGCCGCA
AACCTTTCAGATTCTGGATTCCGCCGATCAGCTGGCGGTCATCAAGCGCATCCTGAAAAATCTGGGTGCGGACGATGAGA
AATTTCCTCCGCGTCAGGTCCAATGGTTCATCAATAATGCCAAGGAGGAGGGGCTGCGTGCATCGCAGGTGGAAGCGTAC
GATGACTACACGCGCAAGATGGTCGAGTTCTATGCATCTTACGAGCACCAATGCAACAAGGAAGGGGTGGTCGATTTTGC
CGAGTTGTTGCTGCGCAGCTATGAATTGCTGATGCGCAACGAAATCGTACGCGAGCATTATCGCAGCCGGTTCCTGCACA
TCCTGGTGGACGAGTTCCAGGATACCAGTCGGCTGCAATACAAATGGCTGCGCCTGCTCGCCGGTCCGAAAACCGCTGTC
TTTGCCGTGGGCGATGACGACCAGAGCATTTATGCCTTCCGTGGCGCCAATACCGGCAACATGAGAGAGTTCGAACAGGA
TTTTCGCATCCCCAGGATCATCAAACTGGAGCAGAACTACCGTTCACATGGCAATATTCTGGATGCTGCCAACACGCTGA
TACGCAACAATAATGGGCGGCTCGGCAAAAATCTCTGGACTTCAGAAGGGCGCGGTGAGCCGATACGGGTATACAACGCT
CCGACGGATTTCGATGAAGCGGCGTTTATCGTGGATGAAGTCAAATCCCTGCGCGCAGAGGGAGTCCAACTGTCCCAGAT
CGCGTTGCTTTATCGTTCCAATGCCCAATCACGTGTGCTGGAGCACGCCTTGTTCAATGCCTCGCTGCCTTACCGGGTAT
ACGGAGGCATGCGCTTCTTCGAGCGACAGGAAATCAAGCACGCATTGGCTTACCTGCGAATGATAGCCAATCCGGAGGAC
GACAACGCGCTACTGAGAATCGTCAACTTCCCGGCACGTGGTATCGGCCTGCGGAGTCTGGAGCAATTGCAGGATGAAGT
CAGGCAACAGGGGGGAAGCCTCTGGGAAGCGATGCTGAAAAAATGCAGGGGCAGAGAACCCGCCACGTCAGGCTTGCTGA
ATAAGTCCTCGGCGGGAGTGGGGGAGAATAAAGAGCCGGACAGGCCCAGAAGAGGCATCGAGGGATTCGTTGCGCTGATC
GAATCGATGCGGCAGGTTTGTGAAACCCTGCCGCTCCCGGAAATTGTCGAACATATGCTGGAGCACAGCGGATTGATCAG
GCACTATCAAGCCGAGCGCGAGGGGGCCGACCGGCTGGAGAATTTGAACGAGTTGGTCACTGCGGCGACTGCCTTCGTGC
ACGAAGCCGAAGATGACGGTCTGGCCGTTTTTCTCAATCATGCTTCACTGGAAGCAGGCGAGCACCAGGCGGGCGGAGGG
GAGGATGCCTTGCAGCTGATGACCGTGCACTCAGCCAAAGGACTGGAGTTTCACAGCGTATTTCTAAGCGGTCTCGAAGA
AGGTTTGTTTCCCCACGATAACAGCCGTAACGAGGCGGGGGGGCTGGAAGAAGAGCGGCGCCTGATGTACGTAGCCGTGA
CGCGCGCGAAGCGCCGGCTGTATTTGAGCTTTGCCCAAAGCCGCATGTTGCATGGCCAGACTCGCTACAACATTCCCACT
CGATTCATAGAGGAAATACCGCAGGGCGTGCTCAAGTGGCTGCAGCCCGTGCAGAAGACAGCGCCTTGGCGACCGGCGCC
GCTTTCTCCCACTCAGGAGGTTGCGCAGCGCCCCCAAATCCGGGAAATGGGAGTGAGGGAGGCGACGGCATCGCCCTGGC
GGGTGGGCCAGAACGTGGTTCACGCAAAGTTCGGGGCAGGAGTGATCGTCAACTGCGAAGGCCGCGGTGCCGATGCGCGG
GTTGAGGTTAAATTCGGGCGCAATGGCAGCAAATGGTTGTTACTGGAATATGCAAAACTGACAGCGTTGTAA

Upstream 100 bases:

>100_bases
GGGTTTGCAGGAGTATCATATTGTTCTACATTGCTTTTAATCCGATTTTGCGCGGCCTGAATCATCCCGGTCGTGCCCGG
ACCGGAAAAATCTTTGCTGT

Downstream 100 bases:

>100_bases
AGCCTGGAAGCCCGGGTGCCCAGGGGGGAGCTGGGAACAGTTTGCTTTCCGCCGTGCATTCCCCGTCATGGATCCCCGGG
GGAATCATGCGCTTTCCGAG

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 743; Mature: 742

Protein sequence:

>743_residues
MSSLLTALNPEQLEAVTLPHQSALVLAGAGSGKTKVLTTRIAYLIQSGEVSPHGILAVTFTNKAAKEMLTRIAAMLPINT
RGMWIGTFHGLCNRLLRAHHQDAGLPQTFQILDSADQLAVIKRILKNLGADDEKFPPRQVQWFINNAKEEGLRASQVEAY
DDYTRKMVEFYASYEHQCNKEGVVDFAELLLRSYELLMRNEIVREHYRSRFLHILVDEFQDTSRLQYKWLRLLAGPKTAV
FAVGDDDQSIYAFRGANTGNMREFEQDFRIPRIIKLEQNYRSHGNILDAANTLIRNNNGRLGKNLWTSEGRGEPIRVYNA
PTDFDEAAFIVDEVKSLRAEGVQLSQIALLYRSNAQSRVLEHALFNASLPYRVYGGMRFFERQEIKHALAYLRMIANPED
DNALLRIVNFPARGIGLRSLEQLQDEVRQQGGSLWEAMLKKCRGREPATSGLLNKSSAGVGENKEPDRPRRGIEGFVALI
ESMRQVCETLPLPEIVEHMLEHSGLIRHYQAEREGADRLENLNELVTAATAFVHEAEDDGLAVFLNHASLEAGEHQAGGG
EDALQLMTVHSAKGLEFHSVFLSGLEEGLFPHDNSRNEAGGLEEERRLMYVAVTRAKRRLYLSFAQSRMLHGQTRYNIPT
RFIEEIPQGVLKWLQPVQKTAPWRPAPLSPTQEVAQRPQIREMGVREATASPWRVGQNVVHAKFGAGVIVNCEGRGADAR
VEVKFGRNGSKWLLLEYAKLTAL

Sequences:

>Translated_743_residues
MSSLLTALNPEQLEAVTLPHQSALVLAGAGSGKTKVLTTRIAYLIQSGEVSPHGILAVTFTNKAAKEMLTRIAAMLPINT
RGMWIGTFHGLCNRLLRAHHQDAGLPQTFQILDSADQLAVIKRILKNLGADDEKFPPRQVQWFINNAKEEGLRASQVEAY
DDYTRKMVEFYASYEHQCNKEGVVDFAELLLRSYELLMRNEIVREHYRSRFLHILVDEFQDTSRLQYKWLRLLAGPKTAV
FAVGDDDQSIYAFRGANTGNMREFEQDFRIPRIIKLEQNYRSHGNILDAANTLIRNNNGRLGKNLWTSEGRGEPIRVYNA
PTDFDEAAFIVDEVKSLRAEGVQLSQIALLYRSNAQSRVLEHALFNASLPYRVYGGMRFFERQEIKHALAYLRMIANPED
DNALLRIVNFPARGIGLRSLEQLQDEVRQQGGSLWEAMLKKCRGREPATSGLLNKSSAGVGENKEPDRPRRGIEGFVALI
ESMRQVCETLPLPEIVEHMLEHSGLIRHYQAEREGADRLENLNELVTAATAFVHEAEDDGLAVFLNHASLEAGEHQAGGG
EDALQLMTVHSAKGLEFHSVFLSGLEEGLFPHDNSRNEAGGLEEERRLMYVAVTRAKRRLYLSFAQSRMLHGQTRYNIPT
RFIEEIPQGVLKWLQPVQKTAPWRPAPLSPTQEVAQRPQIREMGVREATASPWRVGQNVVHAKFGAGVIVNCEGRGADAR
VEVKFGRNGSKWLLLEYAKLTAL
>Mature_742_residues
SSLLTALNPEQLEAVTLPHQSALVLAGAGSGKTKVLTTRIAYLIQSGEVSPHGILAVTFTNKAAKEMLTRIAAMLPINTR
GMWIGTFHGLCNRLLRAHHQDAGLPQTFQILDSADQLAVIKRILKNLGADDEKFPPRQVQWFINNAKEEGLRASQVEAYD
DYTRKMVEFYASYEHQCNKEGVVDFAELLLRSYELLMRNEIVREHYRSRFLHILVDEFQDTSRLQYKWLRLLAGPKTAVF
AVGDDDQSIYAFRGANTGNMREFEQDFRIPRIIKLEQNYRSHGNILDAANTLIRNNNGRLGKNLWTSEGRGEPIRVYNAP
TDFDEAAFIVDEVKSLRAEGVQLSQIALLYRSNAQSRVLEHALFNASLPYRVYGGMRFFERQEIKHALAYLRMIANPEDD
NALLRIVNFPARGIGLRSLEQLQDEVRQQGGSLWEAMLKKCRGREPATSGLLNKSSAGVGENKEPDRPRRGIEGFVALIE
SMRQVCETLPLPEIVEHMLEHSGLIRHYQAEREGADRLENLNELVTAATAFVHEAEDDGLAVFLNHASLEAGEHQAGGGE
DALQLMTVHSAKGLEFHSVFLSGLEEGLFPHDNSRNEAGGLEEERRLMYVAVTRAKRRLYLSFAQSRMLHGQTRYNIPTR
FIEEIPQGVLKWLQPVQKTAPWRPAPLSPTQEVAQRPQIREMGVREATASPWRVGQNVVHAKFGAGVIVNCEGRGADARV
EVKFGRNGSKWLLLEYAKLTAL

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=749, Percent_Identity=48.9986648865153, Blast_Score=689, Evalue=0.0,
Organism=Escherichia coli, GI48994965, Length=662, Percent_Identity=36.5558912386707, Blast_Score=364, Evalue=1e-101,
Organism=Escherichia coli, GI1787196, Length=340, Percent_Identity=30, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6322369, Length=760, Percent_Identity=27.2368421052632, Blast_Score=218, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6324477, Length=688, Percent_Identity=22.2383720930233, Blast_Score=105, Evalue=3e-23,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005753
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 83662; Mature: 83531

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLLTALNPEQLEAVTLPHQSALVLAGAGSGKTKVLTTRIAYLIQSGEVSPHGILAVTF
CCCHHHHCCHHHEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEE
TNKAAKEMLTRIAAMLPINTRGMWIGTFHGLCNRLLRAHHQDAGLPQTFQILDSADQLAV
CCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHH
IKRILKNLGADDEKFPPRQVQWFINNAKEEGLRASQVEAYDDYTRKMVEFYASYEHQCNK
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
EGVVDFAELLLRSYELLMRNEIVREHYRSRFLHILVDEFQDTSRLQYKWLRLLAGPKTAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEE
FAVGDDDQSIYAFRGANTGNMREFEQDFRIPRIIKLEQNYRSHGNILDAANTLIRNNNGR
EEECCCCCCEEEEECCCCCCHHHHHHHCCCCEEEEECHHHHHCCCHHHHHHHHHCCCCCC
LGKNLWTSEGRGEPIRVYNAPTDFDEAAFIVDEVKSLRAEGVQLSQIALLYRSNAQSRVL
CCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHH
EHALFNASLPYRVYGGMRFFERQEIKHALAYLRMIANPEDDNALLRIVNFPARGIGLRSL
HHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHH
EQLQDEVRQQGGSLWEAMLKKCRGREPATSGLLNKSSAGVGENKEPDRPRRGIEGFVALI
HHHHHHHHHCCCHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ESMRQVCETLPLPEIVEHMLEHSGLIRHYQAEREGADRLENLNELVTAATAFVHEAEDDG
HHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LAVFLNHASLEAGEHQAGGGEDALQLMTVHSAKGLEFHSVFLSGLEEGLFPHDNSRNEAG
EEEEEECCCCCCCCCCCCCCHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
GLEEERRLMYVAVTRAKRRLYLSFAQSRMLHGQTRYNIPTRFIEEIPQGVLKWLQPVQKT
CCCHHHHEEHEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
APWRPAPLSPTQEVAQRPQIREMGVREATASPWRVGQNVVHAKFGAGVIVNCEGRGADAR
CCCCCCCCCCHHHHHCCCCHHHCCCHHCCCCCHHHCCHHEEEECCCCEEEEECCCCCCEE
VEVKFGRNGSKWLLLEYAKLTAL
EEEEECCCCCEEEEEEHHHHHCC
>Mature Secondary Structure 
SSLLTALNPEQLEAVTLPHQSALVLAGAGSGKTKVLTTRIAYLIQSGEVSPHGILAVTF
CCHHHHCCHHHEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEE
TNKAAKEMLTRIAAMLPINTRGMWIGTFHGLCNRLLRAHHQDAGLPQTFQILDSADQLAV
CCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHH
IKRILKNLGADDEKFPPRQVQWFINNAKEEGLRASQVEAYDDYTRKMVEFYASYEHQCNK
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
EGVVDFAELLLRSYELLMRNEIVREHYRSRFLHILVDEFQDTSRLQYKWLRLLAGPKTAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEE
FAVGDDDQSIYAFRGANTGNMREFEQDFRIPRIIKLEQNYRSHGNILDAANTLIRNNNGR
EEECCCCCCEEEEECCCCCCHHHHHHHCCCCEEEEECHHHHHCCCHHHHHHHHHCCCCCC
LGKNLWTSEGRGEPIRVYNAPTDFDEAAFIVDEVKSLRAEGVQLSQIALLYRSNAQSRVL
CCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHH
EHALFNASLPYRVYGGMRFFERQEIKHALAYLRMIANPEDDNALLRIVNFPARGIGLRSL
HHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHH
EQLQDEVRQQGGSLWEAMLKKCRGREPATSGLLNKSSAGVGENKEPDRPRRGIEGFVALI
HHHHHHHHHCCCHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ESMRQVCETLPLPEIVEHMLEHSGLIRHYQAEREGADRLENLNELVTAATAFVHEAEDDG
HHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LAVFLNHASLEAGEHQAGGGEDALQLMTVHSAKGLEFHSVFLSGLEEGLFPHDNSRNEAG
EEEEEECCCCCCCCCCCCCCHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
GLEEERRLMYVAVTRAKRRLYLSFAQSRMLHGQTRYNIPTRFIEEIPQGVLKWLQPVQKT
CCCHHHHEEHEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
APWRPAPLSPTQEVAQRPQIREMGVREATASPWRVGQNVVHAKFGAGVIVNCEGRGADAR
CCCCCCCCCCHHHHHCCCCHHHCCCHHCCCCCHHHCCHHEEEECCCCEEEEECCCCCCEE
VEVKFGRNGSKWLLLEYAKLTAL
EEEEECCCCCEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6379604; 2943729; 1379743; 9278503; 6324092; 2254268; 8419285 [H]