Click here to switch to the map view.

The map label for this gene is glyS [H]

Identifier: 82701684

GI number: 82701684

Start: 634166

End: 636385

Strand: Reverse

Name: glyS [H]

Synonym: Nmul_A0550

Alternate gene names: 82701684

Gene position: 636385-634166 (Counterclockwise)

Preceding gene: 82701685

Following gene: 82701683

Centisome position: 19.99

GC content: 57.48

Gene sequence:

>2220_bases
TTGACTGAGGCAACGCAAGCAACCCAAGGTTTGGCAACCCGAAATCTTCTGGTTGAACTGCTGGTAGAGGAACTGCCCCC
CAAGTCGCTGAAGAAACTGGGTGAAAGTTTCGCGCAGGGAATAGCCGCGGGTCTGAAAAGACAGGGCTTGGCGCGGGAAG
ATGCGGCCGCCACCCCTTTTGCCTCTCCGCGACGCCTTGCCGTACATCTCACATGTGTAGCAGCACAGGCGCCGGACACA
TCCTCCCTGAAAAAACTGATGCCGGTAGCAGTGGGTCTGGATGAAAATGGCCAGCCCACCCCTGCCCTGCTCAAGAAGCT
GCGCAGTCTTTGCGGCGATGCTGATCCTGTCCCATTCCTCTCTCAGCTCAAACGCGCCCACGATGGAAAAACAGAAACGC
TGTTTCTGGACAGCGTAGTGAAGGGAGCGACGCTGGCGGAAGGCCTCCAGACAGCGCTGGCCGGAACGCTGGAGAAATTG
CCTATTGCCAGAATGATGACATATCAACTCGCGGACGGCTGGAATACCATCAATTTCGTCCGCCCGGCGCATGCGCTCGT
TGCCCTGCATGGGAAAGAAGTCGTGGATGTATCCATATTGGGCCTGACAGCGGGGCGTGAAACGCAAGGACACCGTTTCG
AAGCGATGACGAGTCCCCTGATCGTGAAAGATGCGGACAGTTACGAGCATCAGTTACAAGAAGAAGGGGCGGTGATTGCA
AGCTTCTCCCATCGTCGCGCGGAAATCGAGCGCCAGTTGCTGGCCGCCGCCCGCAAAGAAGATCTCAAATTGACCCGGTA
TGATGCCCTGCTGGATGAAGTTGCCGCGCTGGTGGAACGTCCCAGGGTACTGACGGGTGAATTTGAAACGGGATTCCTGG
ATGTGCCCCAGGAGTGCCTGATTCTGACGATGCAGGCCAATCAGAAGTATTTCCCTCTGCTCGATGCGCGCGGCCAACTC
TCGCACCGGTTCCTGATCGTATCCAATATCCGCCCGGTGGATGCGCGCTCAGTGATTGCGGGGAACGAACGCGTACTGCG
CTCGCGCCTGGCGGACGCAAAATTCTTTTTCGACCAGGATCGCAGGAAGACACTGGCCTCGCGAGTGGAAGGCCTGCAGC
GGGTGGTATACCACAACAAGCTGGGTACGCAGGCGGAACGGGTGGAAAGGATCTGGGCCATTGCACAAACCATCGGCTTA
CAGCTTGGCGGCAATGAACTCGCAGCCAGGGCGGGCGAAGCAGCCATGCTCGCCAAAGCGGATCTGCTCACCGACATGGT
TGGCGAGTTCCCGGAGTTGCAGGGCATCATGGGACGCTATTATGCAAAGCATGACGGCGTGGCAGAAGAGGTCGCCGACG
CGATCGAAGACCACTACAAGCCGCGCTTTGCAGGGGATGAATTACCGCGCAATGCGGTTGGCGTATGCGTTGCGCTCGCC
GACAAGCTCGAAACACTGGTCGGCCTGTTCGGCATTGGCCAGATACCCACTGGAGACAAAGATCCCTTCGCGTTGCGCCG
TCACGCGCTGGGTGTAATCCGCATATTGATCGAAAAGGAAGTGCCTCTGGCGCTGAACATCCTGCTCGAGCAGACGGCGG
ACATTTTTGGACAAAGAATCAAAGGCTCAGCTGAAACCATAGCTAAAACCGCCACTGAAACTTTAGAGGGTTTCATTTAC
GAACGGCTGAAGCACTATTTCAGTGCCGAAGCCATCGAGGGGCAGATAACGGGCTTTGGCAGATCCCCTAACGAGGACCG
GTCAACTTTTTCCCCGCAGCAGATCGACGCGGTACTCGCCCTGCGCCCCCAGTTGCTGAATGATGTCAAAAAACGCCTGC
TCGCCGTGCGTGCTTTCGCCGCCCTGCCGGAAGCCGAAAGTCTTGCAGCGACCAATAAGCGGGTGGGCAACATTCTCAGA
AAAGCGGAAGTCGACGCAGGCGGGGAAATCGACACCGACTTGTTGCAGACCCCTGCGGAACAGGCGCTGCACCAGGCATT
AGACCGTATAATACCGGAAGCCGATGCAGCCTTTGCTGCCGGCGACTATACCGCTTCGCTGCAGGCGCTGGCTGCACTGA
AAGTTCCGGTGGACCAGTTTTTCGATACAGTAATGGTGAATACGGAGGATGCTGCGCTGCGCCATAACCGCCTGGCCCTG
CTTGCGCAAATGCAGAAGATAATGAACCGGATTGCGGATATTTCCCGGCTGGCGCTCTGA

Upstream 100 bases:

>100_bases
TCGCACGCAGCGTGGCGCACGCCTATTACGAAAGCCGCAAGCGCCTGGGCTTTCCCCTGGCGCCGGTGGAATGGACGCAG
GAATTGCTGGAGAAAGCCGC

Downstream 100 bases:

>100_bases
TACCGGCAACCGATCATGAAACTCGTCATTCTCGACCGGGACGGCGTCATCAATTACGACAGTGCCGCCTTTATCAAGAC
GCCGGACGAATGGAAGCCGA

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 739; Mature: 738

Protein sequence:

>739_residues
MTEATQATQGLATRNLLVELLVEELPPKSLKKLGESFAQGIAAGLKRQGLAREDAAATPFASPRRLAVHLTCVAAQAPDT
SSLKKLMPVAVGLDENGQPTPALLKKLRSLCGDADPVPFLSQLKRAHDGKTETLFLDSVVKGATLAEGLQTALAGTLEKL
PIARMMTYQLADGWNTINFVRPAHALVALHGKEVVDVSILGLTAGRETQGHRFEAMTSPLIVKDADSYEHQLQEEGAVIA
SFSHRRAEIERQLLAAARKEDLKLTRYDALLDEVAALVERPRVLTGEFETGFLDVPQECLILTMQANQKYFPLLDARGQL
SHRFLIVSNIRPVDARSVIAGNERVLRSRLADAKFFFDQDRRKTLASRVEGLQRVVYHNKLGTQAERVERIWAIAQTIGL
QLGGNELAARAGEAAMLAKADLLTDMVGEFPELQGIMGRYYAKHDGVAEEVADAIEDHYKPRFAGDELPRNAVGVCVALA
DKLETLVGLFGIGQIPTGDKDPFALRRHALGVIRILIEKEVPLALNILLEQTADIFGQRIKGSAETIAKTATETLEGFIY
ERLKHYFSAEAIEGQITGFGRSPNEDRSTFSPQQIDAVLALRPQLLNDVKKRLLAVRAFAALPEAESLAATNKRVGNILR
KAEVDAGGEIDTDLLQTPAEQALHQALDRIIPEADAAFAAGDYTASLQALAALKVPVDQFFDTVMVNTEDAALRHNRLAL
LAQMQKIMNRIADISRLAL

Sequences:

>Translated_739_residues
MTEATQATQGLATRNLLVELLVEELPPKSLKKLGESFAQGIAAGLKRQGLAREDAAATPFASPRRLAVHLTCVAAQAPDT
SSLKKLMPVAVGLDENGQPTPALLKKLRSLCGDADPVPFLSQLKRAHDGKTETLFLDSVVKGATLAEGLQTALAGTLEKL
PIARMMTYQLADGWNTINFVRPAHALVALHGKEVVDVSILGLTAGRETQGHRFEAMTSPLIVKDADSYEHQLQEEGAVIA
SFSHRRAEIERQLLAAARKEDLKLTRYDALLDEVAALVERPRVLTGEFETGFLDVPQECLILTMQANQKYFPLLDARGQL
SHRFLIVSNIRPVDARSVIAGNERVLRSRLADAKFFFDQDRRKTLASRVEGLQRVVYHNKLGTQAERVERIWAIAQTIGL
QLGGNELAARAGEAAMLAKADLLTDMVGEFPELQGIMGRYYAKHDGVAEEVADAIEDHYKPRFAGDELPRNAVGVCVALA
DKLETLVGLFGIGQIPTGDKDPFALRRHALGVIRILIEKEVPLALNILLEQTADIFGQRIKGSAETIAKTATETLEGFIY
ERLKHYFSAEAIEGQITGFGRSPNEDRSTFSPQQIDAVLALRPQLLNDVKKRLLAVRAFAALPEAESLAATNKRVGNILR
KAEVDAGGEIDTDLLQTPAEQALHQALDRIIPEADAAFAAGDYTASLQALAALKVPVDQFFDTVMVNTEDAALRHNRLAL
LAQMQKIMNRIADISRLAL
>Mature_738_residues
TEATQATQGLATRNLLVELLVEELPPKSLKKLGESFAQGIAAGLKRQGLAREDAAATPFASPRRLAVHLTCVAAQAPDTS
SLKKLMPVAVGLDENGQPTPALLKKLRSLCGDADPVPFLSQLKRAHDGKTETLFLDSVVKGATLAEGLQTALAGTLEKLP
IARMMTYQLADGWNTINFVRPAHALVALHGKEVVDVSILGLTAGRETQGHRFEAMTSPLIVKDADSYEHQLQEEGAVIAS
FSHRRAEIERQLLAAARKEDLKLTRYDALLDEVAALVERPRVLTGEFETGFLDVPQECLILTMQANQKYFPLLDARGQLS
HRFLIVSNIRPVDARSVIAGNERVLRSRLADAKFFFDQDRRKTLASRVEGLQRVVYHNKLGTQAERVERIWAIAQTIGLQ
LGGNELAARAGEAAMLAKADLLTDMVGEFPELQGIMGRYYAKHDGVAEEVADAIEDHYKPRFAGDELPRNAVGVCVALAD
KLETLVGLFGIGQIPTGDKDPFALRRHALGVIRILIEKEVPLALNILLEQTADIFGQRIKGSAETIAKTATETLEGFIYE
RLKHYFSAEAIEGQITGFGRSPNEDRSTFSPQQIDAVLALRPQLLNDVKKRLLAVRAFAALPEAESLAATNKRVGNILRK
AEVDAGGEIDTDLLQTPAEQALHQALDRIIPEADAAFAAGDYTASLQALAALKVPVDQFFDTVMVNTEDAALRHNRLALL
AQMQKIMNRIADISRLAL

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=728, Percent_Identity=39.8351648351648, Blast_Score=475, Evalue=1e-135,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 80691; Mature: 80560

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEATQATQGLATRNLLVELLVEELPPKSLKKLGESFAQGIAAGLKRQGLAREDAAATPF
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
ASPRRLAVHLTCVAAQAPDTSSLKKLMPVAVGLDENGQPTPALLKKLRSLCGDADPVPFL
CCCCEEEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHH
SQLKRAHDGKTETLFLDSVVKGATLAEGLQTALAGTLEKLPIARMMTYQLADGWNTINFV
HHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHC
RPAHALVALHGKEVVDVSILGLTAGRETQGHRFEAMTSPLIVKDADSYEHQLQEEGAVIA
CHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCHHHHCCCEEEECCCHHHHHHHHCCCEEE
SFSHRRAEIERQLLAAARKEDLKLTRYDALLDEVAALVERPRVLTGEFETGFLDVPQECL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHE
ILTMQANQKYFPLLDARGQLSHRFLIVSNIRPVDARSVIAGNERVLRSRLADAKFFFDQD
EEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHH
RRKTLASRVEGLQRVVYHNKLGTQAERVERIWAIAQTIGLQLGGNELAARAGEAAMLAKA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHH
DLLTDMVGEFPELQGIMGRYYAKHDGVAEEVADAIEDHYKPRFAGDELPRNAVGVCVALA
HHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
DKLETLVGLFGIGQIPTGDKDPFALRRHALGVIRILIEKEVPLALNILLEQTADIFGQRI
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
KGSAETIAKTATETLEGFIYERLKHYFSAEAIEGQITGFGRSPNEDRSTFSPQQIDAVLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHH
LRPQLLNDVKKRLLAVRAFAALPEAESLAATNKRVGNILRKAEVDAGGEIDTDLLQTPAE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCHHH
QALHQALDRIIPEADAAFAAGDYTASLQALAALKVPVDQFFDTVMVNTEDAALRHNRLAL
HHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHH
LAQMQKIMNRIADISRLAL
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEATQATQGLATRNLLVELLVEELPPKSLKKLGESFAQGIAAGLKRQGLAREDAAATPF
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
ASPRRLAVHLTCVAAQAPDTSSLKKLMPVAVGLDENGQPTPALLKKLRSLCGDADPVPFL
CCCCEEEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHH
SQLKRAHDGKTETLFLDSVVKGATLAEGLQTALAGTLEKLPIARMMTYQLADGWNTINFV
HHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHC
RPAHALVALHGKEVVDVSILGLTAGRETQGHRFEAMTSPLIVKDADSYEHQLQEEGAVIA
CHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCHHHHCCCEEEECCCHHHHHHHHCCCEEE
SFSHRRAEIERQLLAAARKEDLKLTRYDALLDEVAALVERPRVLTGEFETGFLDVPQECL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHE
ILTMQANQKYFPLLDARGQLSHRFLIVSNIRPVDARSVIAGNERVLRSRLADAKFFFDQD
EEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHH
RRKTLASRVEGLQRVVYHNKLGTQAERVERIWAIAQTIGLQLGGNELAARAGEAAMLAKA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHH
DLLTDMVGEFPELQGIMGRYYAKHDGVAEEVADAIEDHYKPRFAGDELPRNAVGVCVALA
HHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
DKLETLVGLFGIGQIPTGDKDPFALRRHALGVIRILIEKEVPLALNILLEQTADIFGQRI
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
KGSAETIAKTATETLEGFIYERLKHYFSAEAIEGQITGFGRSPNEDRSTFSPQQIDAVLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHH
LRPQLLNDVKKRLLAVRAFAALPEAESLAATNKRVGNILRKAEVDAGGEIDTDLLQTPAE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCHHH
QALHQALDRIIPEADAAFAAGDYTASLQALAALKVPVDQFFDTVMVNTEDAALRHNRLAL
HHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHH
LAQMQKIMNRIADISRLAL
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA