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The map label for this gene is thrS

Identifier: 82701621

GI number: 82701621

Start: 545984

End: 547900

Strand: Direct

Name: thrS

Synonym: Nmul_A0487

Alternate gene names: 82701621

Gene position: 545984-547900 (Clockwise)

Preceding gene: 82701620

Following gene: 82701622

Centisome position: 17.15

GC content: 54.88

Gene sequence:

>1917_bases
GTGGCTGTAGTTCGTTTGCCGGATGGTTCGGAGCGCGTGTATGAGCATTCTGTAACGGTGGGTGAAGTTGCAGCTTCGAT
AGGCGCCGGGCTGGCTCGCGCTGCGCTTGCAGGCAAGGTGAACGGCAAACTGGTTGACCTTTCCGCTCGTATCGAAAATG
ACAGCGACGTAGCCATCATCACGGAAAAGGATGCGGAAGGGCTCGAGATCATCCGGCATTCCAGCGCCCATTTGCTGGCG
CATGCGGTGAAAGAATTGTTTCCGGAAGCGCAGGTCACGATTGGACCGGTAATCGAGGACGGATTTTATTACGATTTTTC
CTATAAGCGACCCTTTACCCCCGAAGATCTGGCGGCAATCGAGAAACGCATGGCTGAAATCAGCACGCGCAACCTGAAGG
TCGAGCGCCAGGTTTGGGATCGATCCGAGGCAATCAATTTTTTCAAGAACCAGGGCGAACACTATAAGGCGCAGATCATC
GAAGCCATTCCCGGCGATGAGGACGTTTCCCTGTATTCTCAGGGAAATTTTACCGATTTATGCCGCGGTCCCCACGTCCC
GGCCACATCCCGTCTGAAGGTTTTCAAGCTGATGAAGCTTGCAGGCGCCTATTGGCGTGGGGATTCGCATAACGAAATGC
TGCAGCGGATCTATGGCACCGCATGGACCAATAAAGACGATCAGAACGCCTATCTGCGCCGTCTTGAAGAGGCGGAGAAA
CGCGATCACCGCAAGCTCGGCAAGCAACTGGGTCTGTTTCACCTTCAGGAAGAAGCGCCTGGAATGGTGTTCTGGCACCC
GAAGGGATGGGTCGTCTGGCAGCAGGTCGAACAGTACATGCGGGACATCTTTCGTAATAACGGCTATCTCGAAATCCGCA
CGCCCTCCGTACTGGACAAGGGTTTGTGGGAGCGTTCCGGACACTGGGAAAACTTCCGCGAGAACATGTTCGTGACGCAG
GCCGAAGATCGGGAATTTTCCGTCAAGCCGATGAATTGTCCGGGGCATGTGCAGGTGTTCAAGCAGGGGCTGAAAAGTTA
TCGCGACCTGCCGCTCCGGCTCGCGGAATTCGGTTCCTGTCACCGTAACGAGCCCTCCGGAGCGCTGCACGGGATCATGC
GGGTGCGAGCGTTCACTCAGGACGACGCTCACATTTTCTGCACTGAGTCCCAGGTGCAAGATGAGGCCGTCCAGTTTATC
GATCTGTTGCAGAAGGTTTACAACGATTTTGGCTTTAATGACATCCTGGTGAAACTTTCCACCCGTCCCGCAAAGCGCGT
AGGCTCCGAAGAGCAGTGGGACAAGGCGGAAGAGGCGCTGCGATCCGCGCTGAATCACAAAAACCTCATCTGGGAACTGC
AACCGGGTGAAGGTGCGTTCTACGGACCAAAAATAGAGTTTTCGCTCAAGGATAGTATCGGTCGCATCTGGCAGTGCGGC
ACCTTGCAACTGGATTTTTCCATGCCGGAACGCCTCGGCGCAGAGTTTGTGGCCGAGGATAACTCCCGGCAGATACCGGT
AATGCTTCACCGCGCAATCCTCGGCTCCCTGGAACGTTTTATCGGCATTCTTATCGAAAATCATGCGGGAGCACTGCCGC
TATGGTTGTCCCCGGACCATGCGGTCGTGCTGAATATCTCGGAAGGGCAGGCCGATTATGCCCGAGAAGTGACCGAAGAA
CTCAGAAGAGCGGGAATTCGAGCGTATGCGGACTTGAGAAATGAAAAAATAACTTATAAAATACGGGAGCATAGTCTGCA
AAAACTGCCCTATCAAATCATCGTGGGTGATAAGGAAGTGGCAGCCCAGAGAGTGGCTGTACGTACCCGCAGTGGCTCTG
ACCTTGGTCAGATGACATTGCCGGCATTGGTGGACCGGCTTAAGGAAGAGATTCGCACCCGGGCGGGGGCGGCCTGA

Upstream 100 bases:

>100_bases
GCGCGACCGCTTTTCCGGCACCTCGCCGTCCCACCCCCGTGCCGCTGAATCCTGTAAAATCAGGGTTTTTAGTCAGTCTT
ACTTATCAAAGGGAGAAGCC

Downstream 100 bases:

>100_bases
TTTATTAATAGAGTTCGGGAGTTACTATAGTTCAAGAAAAAGCAGCACGCATCAATTACGAGATTACTGCGCCGGAAGTG
CGCTTGATTGGTGCGGATGG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 638; Mature: 637

Protein sequence:

>638_residues
MAVVRLPDGSERVYEHSVTVGEVAASIGAGLARAALAGKVNGKLVDLSARIENDSDVAIITEKDAEGLEIIRHSSAHLLA
HAVKELFPEAQVTIGPVIEDGFYYDFSYKRPFTPEDLAAIEKRMAEISTRNLKVERQVWDRSEAINFFKNQGEHYKAQII
EAIPGDEDVSLYSQGNFTDLCRGPHVPATSRLKVFKLMKLAGAYWRGDSHNEMLQRIYGTAWTNKDDQNAYLRRLEEAEK
RDHRKLGKQLGLFHLQEEAPGMVFWHPKGWVVWQQVEQYMRDIFRNNGYLEIRTPSVLDKGLWERSGHWENFRENMFVTQ
AEDREFSVKPMNCPGHVQVFKQGLKSYRDLPLRLAEFGSCHRNEPSGALHGIMRVRAFTQDDAHIFCTESQVQDEAVQFI
DLLQKVYNDFGFNDILVKLSTRPAKRVGSEEQWDKAEEALRSALNHKNLIWELQPGEGAFYGPKIEFSLKDSIGRIWQCG
TLQLDFSMPERLGAEFVAEDNSRQIPVMLHRAILGSLERFIGILIENHAGALPLWLSPDHAVVLNISEGQADYAREVTEE
LRRAGIRAYADLRNEKITYKIREHSLQKLPYQIIVGDKEVAAQRVAVRTRSGSDLGQMTLPALVDRLKEEIRTRAGAA

Sequences:

>Translated_638_residues
MAVVRLPDGSERVYEHSVTVGEVAASIGAGLARAALAGKVNGKLVDLSARIENDSDVAIITEKDAEGLEIIRHSSAHLLA
HAVKELFPEAQVTIGPVIEDGFYYDFSYKRPFTPEDLAAIEKRMAEISTRNLKVERQVWDRSEAINFFKNQGEHYKAQII
EAIPGDEDVSLYSQGNFTDLCRGPHVPATSRLKVFKLMKLAGAYWRGDSHNEMLQRIYGTAWTNKDDQNAYLRRLEEAEK
RDHRKLGKQLGLFHLQEEAPGMVFWHPKGWVVWQQVEQYMRDIFRNNGYLEIRTPSVLDKGLWERSGHWENFRENMFVTQ
AEDREFSVKPMNCPGHVQVFKQGLKSYRDLPLRLAEFGSCHRNEPSGALHGIMRVRAFTQDDAHIFCTESQVQDEAVQFI
DLLQKVYNDFGFNDILVKLSTRPAKRVGSEEQWDKAEEALRSALNHKNLIWELQPGEGAFYGPKIEFSLKDSIGRIWQCG
TLQLDFSMPERLGAEFVAEDNSRQIPVMLHRAILGSLERFIGILIENHAGALPLWLSPDHAVVLNISEGQADYAREVTEE
LRRAGIRAYADLRNEKITYKIREHSLQKLPYQIIVGDKEVAAQRVAVRTRSGSDLGQMTLPALVDRLKEEIRTRAGAA
>Mature_637_residues
AVVRLPDGSERVYEHSVTVGEVAASIGAGLARAALAGKVNGKLVDLSARIENDSDVAIITEKDAEGLEIIRHSSAHLLAH
AVKELFPEAQVTIGPVIEDGFYYDFSYKRPFTPEDLAAIEKRMAEISTRNLKVERQVWDRSEAINFFKNQGEHYKAQIIE
AIPGDEDVSLYSQGNFTDLCRGPHVPATSRLKVFKLMKLAGAYWRGDSHNEMLQRIYGTAWTNKDDQNAYLRRLEEAEKR
DHRKLGKQLGLFHLQEEAPGMVFWHPKGWVVWQQVEQYMRDIFRNNGYLEIRTPSVLDKGLWERSGHWENFRENMFVTQA
EDREFSVKPMNCPGHVQVFKQGLKSYRDLPLRLAEFGSCHRNEPSGALHGIMRVRAFTQDDAHIFCTESQVQDEAVQFID
LLQKVYNDFGFNDILVKLSTRPAKRVGSEEQWDKAEEALRSALNHKNLIWELQPGEGAFYGPKIEFSLKDSIGRIWQCGT
LQLDFSMPERLGAEFVAEDNSRQIPVMLHRAILGSLERFIGILIENHAGALPLWLSPDHAVVLNISEGQADYAREVTEEL
RRAGIRAYADLRNEKITYKIREHSLQKLPYQIIVGDKEVAAQRVAVRTRSGSDLGQMTLPALVDRLKEEIRTRAGAA

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38570111, Length=639, Percent_Identity=38.3411580594679, Blast_Score=465, Evalue=1e-131,
Organism=Homo sapiens, GI38202255, Length=637, Percent_Identity=38.4615384615385, Blast_Score=460, Evalue=1e-129,
Organism=Homo sapiens, GI20070344, Length=656, Percent_Identity=37.0426829268293, Blast_Score=420, Evalue=1e-117,
Organism=Escherichia coli, GI1788013, Length=636, Percent_Identity=58.3333333333333, Blast_Score=811, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=158, Percent_Identity=29.1139240506329, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71984184, Length=631, Percent_Identity=38.1933438985737, Blast_Score=469, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI71984192, Length=631, Percent_Identity=38.1933438985737, Blast_Score=469, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6322112, Length=612, Percent_Identity=39.8692810457516, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6322654, Length=392, Percent_Identity=38.265306122449, Blast_Score=293, Evalue=6e-80,
Organism=Drosophila melanogaster, GI24583842, Length=629, Percent_Identity=38.6327503974563, Blast_Score=477, Evalue=1e-134,
Organism=Drosophila melanogaster, GI28574141, Length=629, Percent_Identity=38.6327503974563, Blast_Score=477, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24583839, Length=629, Percent_Identity=38.6327503974563, Blast_Score=476, Evalue=1e-134,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_NITMU (Q2YBS6)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411187.1
- ProteinModelPortal:   Q2YBS6
- SMR:   Q2YBS6
- STRING:   Q2YBS6
- GeneID:   3784904
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0487
- eggNOG:   COG0441
- HOGENOM:   HBG352811
- OMA:   TAKDPEC
- PhylomeDB:   Q2YBS6
- ProtClustDB:   PRK00413
- BioCyc:   NMUL323848:NMUL_A0487-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 72534; Mature: 72403

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVRLPDGSERVYEHSVTVGEVAASIGAGLARAALAGKVNGKLVDLSARIENDSDVAII
CEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEE
TEKDAEGLEIIRHSSAHLLAHAVKELFPEAQVTIGPVIEDGFYYDFSYKRPFTPEDLAAI
ECCCCCHHHHHHCCHHHHHHHHHHHHCCCCCEEECCEEECCEEEEECCCCCCCHHHHHHH
EKRMAEISTRNLKVERQVWDRSEAINFFKNQGEHYKAQIIEAIPGDEDVSLYSQGNFTDL
HHHHHHHHHCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCCHHH
CRGPHVPATSRLKVFKLMKLAGAYWRGDSHNEMLQRIYGTAWTNKDDQNAYLRRLEEAEK
HCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
RDHRKLGKQLGLFHLQEEAPGMVFWHPKGWVVWQQVEQYMRDIFRNNGYLEIRTPSVLDK
HHHHHHHHHHCCEEECCCCCCEEEECCCCCEEHHHHHHHHHHHHCCCCEEEEECHHHHHH
GLWERSGHWENFRENMFVTQAEDREFSVKPMNCPGHVQVFKQGLKSYRDLPLRLAEFGSC
HHHHCCCCHHHHHHCEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHCCC
HRNEPSGALHGIMRVRAFTQDDAHIFCTESQVQDEAVQFIDLLQKVYNDFGFNDILVKLS
CCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
TRPAKRVGSEEQWDKAEEALRSALNHKNLIWELQPGEGAFYGPKIEFSLKDSIGRIWQCG
CCCHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEECCEEEEEEHHCHHHHEECC
TLQLDFSMPERLGAEFVAEDNSRQIPVMLHRAILGSLERFIGILIENHAGALPLWLSPDH
EEEECCCCHHHCCCHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
AVVLNISEGQADYAREVTEELRRAGIRAYADLRNEKITYKIREHSLQKLPYQIIVGDKEV
EEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEHHHHHHHCCEEEEECCHHH
AAQRVAVRTRSGSDLGQMTLPALVDRLKEEIRTRAGAA
HHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AVVRLPDGSERVYEHSVTVGEVAASIGAGLARAALAGKVNGKLVDLSARIENDSDVAII
EEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEE
TEKDAEGLEIIRHSSAHLLAHAVKELFPEAQVTIGPVIEDGFYYDFSYKRPFTPEDLAAI
ECCCCCHHHHHHCCHHHHHHHHHHHHCCCCCEEECCEEECCEEEEECCCCCCCHHHHHHH
EKRMAEISTRNLKVERQVWDRSEAINFFKNQGEHYKAQIIEAIPGDEDVSLYSQGNFTDL
HHHHHHHHHCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCCHHH
CRGPHVPATSRLKVFKLMKLAGAYWRGDSHNEMLQRIYGTAWTNKDDQNAYLRRLEEAEK
HCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
RDHRKLGKQLGLFHLQEEAPGMVFWHPKGWVVWQQVEQYMRDIFRNNGYLEIRTPSVLDK
HHHHHHHHHHCCEEECCCCCCEEEECCCCCEEHHHHHHHHHHHHCCCCEEEEECHHHHHH
GLWERSGHWENFRENMFVTQAEDREFSVKPMNCPGHVQVFKQGLKSYRDLPLRLAEFGSC
HHHHCCCCHHHHHHCEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHCCC
HRNEPSGALHGIMRVRAFTQDDAHIFCTESQVQDEAVQFIDLLQKVYNDFGFNDILVKLS
CCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
TRPAKRVGSEEQWDKAEEALRSALNHKNLIWELQPGEGAFYGPKIEFSLKDSIGRIWQCG
CCCHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEECCEEEEEEHHCHHHHEECC
TLQLDFSMPERLGAEFVAEDNSRQIPVMLHRAILGSLERFIGILIENHAGALPLWLSPDH
EEEECCCCHHHCCCHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
AVVLNISEGQADYAREVTEELRRAGIRAYADLRNEKITYKIREHSLQKLPYQIIVGDKEV
EEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEHHHHHHHCCEEEEECCHHH
AAQRVAVRTRSGSDLGQMTLPALVDRLKEEIRTRAGAA
HHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA