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The map label for this gene is carA [H]

Identifier: 82701612

GI number: 82701612

Start: 533840

End: 534991

Strand: Direct

Name: carA [H]

Synonym: Nmul_A0478

Alternate gene names: 82701612

Gene position: 533840-534991 (Clockwise)

Preceding gene: 82701611

Following gene: 82701613

Centisome position: 16.77

GC content: 55.12

Gene sequence:

>1152_bases
GTGCCACAACATTCGTATGCCGTCCTCGCGCTTGCTGACGGCACAGTTTTTCGCGGCATGTCCATCGGCGCGGAAGGCAT
CAGCACAGGGGAGGTGGTCTTCAACACCTCGATGACGGGATATCAGGAAATTCTCACGGATCCCTCCTACTGTCGCCAGA
TCGTTACCCTCACTTATCCCCATATAGGCAATACAGGCACCAATCCGGATGACGTGGAGTCCGGCAATATCGACCGGGTA
TATGCCGCCGGGTTGGTGATAAGAGATCTGCCGTTGGCTTCCAGCAACTGGCGCGAAACGCAGACGTTGCCAGAGTATCT
CAGGCAGCAGGGGGTGGTCGCCATCGCCGACATCGATACCCGCAAGCTCACACGCATTCTGCGGGAAAAGGGCGCTCAGG
CAGGCTGCATCATTGCGGGTCAGCCGGATGAAACGGAGGCGCTGAAGCTGGCCAGGGAATTTCCGGGTCTTGCCGGAATG
GACCTGGCCAAAGTGGTAAGCTGCCTGCAGCCTTATGAGTGGAACGAAGGGGAATGGCGGCTGGGGCAGGGTTACCCGGT
TCAATCTCATCCCCGTTTTCATGTTGCAGCGATGGATTTCGGAATAAAACGAAACATTCTGCGCAAGCTTGCACAGCGCG
GCTGCCAAATCACCGTATTCCCCGCGCAGACAACCGCGGACGAGATTCTGGCATCGCAACCTGACGGAATATTCCTTTCG
AATGGACCGGGTGATCCCGAGCCTTGCGACTACGCTATCGAGACAACCAGAAGACTGCTGGAAAAGGAAATACCGGTTTT
CGGCGTGTGCATGGGTCACCAGTTGCTAGGGCTGGCAACAGGCGCCAGAACCATAAAAATGAAATTTGGCCATCACGGCG
CCAACCATCCGGTTCAGGATCTGGGGACGGGAAAAGTGATCATAACCAGTCAGAATCACGGGTTTGCGGTGGATACGGCC
ACTTTGCCGGCAAATGCAAGAGTTACGCATTTATCTTTATTCGATGGCAGCTTGCAAGGATTTGAGTTGGCGGGAAAGCC
TGTATTCTGCTTTCAGGGGCATCCCGAAGCGAGTCCGGGTCCGCATGACCTGGACTATCTGTTCGATAAATTTGCGGGAT
TGATGGAGAGAAAACGGACGAAAGCGATATGA

Upstream 100 bases:

>100_bases
TTGAAGCATTGTCCTTTTGGACGTATAATTTCCGTATTTGAAGCGGCGCAAAGGGAACCTCGCGCCGTTTCTGCCTTGTC
CCAATTCCCAGGAGTCTTCC

Downstream 100 bases:

>100_bases
AGCCGGAAAGCCATCCGCTCTTTCCCATTATCGTCCCTCCCACTTATGCCTAAACGTAACGACATCAAGTCCATCCTTAT
CATTGGCGCCGGTCCCATCA

Product: carbamoyl-phosphate synthase, small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MPQHSYAVLALADGTVFRGMSIGAEGISTGEVVFNTSMTGYQEILTDPSYCRQIVTLTYPHIGNTGTNPDDVESGNIDRV
YAAGLVIRDLPLASSNWRETQTLPEYLRQQGVVAIADIDTRKLTRILREKGAQAGCIIAGQPDETEALKLAREFPGLAGM
DLAKVVSCLQPYEWNEGEWRLGQGYPVQSHPRFHVAAMDFGIKRNILRKLAQRGCQITVFPAQTTADEILASQPDGIFLS
NGPGDPEPCDYAIETTRRLLEKEIPVFGVCMGHQLLGLATGARTIKMKFGHHGANHPVQDLGTGKVIITSQNHGFAVDTA
TLPANARVTHLSLFDGSLQGFELAGKPVFCFQGHPEASPGPHDLDYLFDKFAGLMERKRTKAI

Sequences:

>Translated_383_residues
MPQHSYAVLALADGTVFRGMSIGAEGISTGEVVFNTSMTGYQEILTDPSYCRQIVTLTYPHIGNTGTNPDDVESGNIDRV
YAAGLVIRDLPLASSNWRETQTLPEYLRQQGVVAIADIDTRKLTRILREKGAQAGCIIAGQPDETEALKLAREFPGLAGM
DLAKVVSCLQPYEWNEGEWRLGQGYPVQSHPRFHVAAMDFGIKRNILRKLAQRGCQITVFPAQTTADEILASQPDGIFLS
NGPGDPEPCDYAIETTRRLLEKEIPVFGVCMGHQLLGLATGARTIKMKFGHHGANHPVQDLGTGKVIITSQNHGFAVDTA
TLPANARVTHLSLFDGSLQGFELAGKPVFCFQGHPEASPGPHDLDYLFDKFAGLMERKRTKAI
>Mature_382_residues
PQHSYAVLALADGTVFRGMSIGAEGISTGEVVFNTSMTGYQEILTDPSYCRQIVTLTYPHIGNTGTNPDDVESGNIDRVY
AAGLVIRDLPLASSNWRETQTLPEYLRQQGVVAIADIDTRKLTRILREKGAQAGCIIAGQPDETEALKLAREFPGLAGMD
LAKVVSCLQPYEWNEGEWRLGQGYPVQSHPRFHVAAMDFGIKRNILRKLAQRGCQITVFPAQTTADEILASQPDGIFLSN
GPGDPEPCDYAIETTRRLLEKEIPVFGVCMGHQLLGLATGARTIKMKFGHHGANHPVQDLGTGKVIITSQNHGFAVDTAT
LPANARVTHLSLFDGSLQGFELAGKPVFCFQGHPEASPGPHDLDYLFDKFAGLMERKRTKAI

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=374, Percent_Identity=41.4438502673797, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI169790915, Length=388, Percent_Identity=34.5360824742268, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI21361331, Length=388, Percent_Identity=34.5360824742268, Blast_Score=216, Evalue=3e-56,
Organism=Escherichia coli, GI1786215, Length=380, Percent_Identity=65, Blast_Score=505, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI193204318, Length=387, Percent_Identity=35.9173126614987, Blast_Score=241, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6324878, Length=388, Percent_Identity=37.1134020618557, Blast_Score=245, Evalue=8e-66,
Organism=Saccharomyces cerevisiae, GI6322331, Length=402, Percent_Identity=35.3233830845771, Blast_Score=223, Evalue=4e-59,
Organism=Drosophila melanogaster, GI45555749, Length=386, Percent_Identity=41.1917098445596, Blast_Score=252, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24642586, Length=386, Percent_Identity=41.1917098445596, Blast_Score=252, Evalue=4e-67,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41744; Mature: 41613

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQHSYAVLALADGTVFRGMSIGAEGISTGEVVFNTSMTGYQEILTDPSYCRQIVTLTYP
CCCCCEEEEEEECCCEECCCCCCCCCCCCCCEEEECCCCHHHHHHCCHHHHHHHHHEECC
HIGNTGTNPDDVESGNIDRVYAAGLVIRDLPLASSNWRETQTLPEYLRQQGVVAIADIDT
CCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEECCH
RKLTRILREKGAQAGCIIAGQPDETEALKLAREFPGLAGMDLAKVVSCLQPYEWNEGEWR
HHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCEE
LGQGYPVQSHPRFHVAAMDFGIKRNILRKLAQRGCQITVFPAQTTADEILASQPDGIFLS
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCEEEE
NGPGDPEPCDYAIETTRRLLEKEIPVFGVCMGHQLLGLATGARTIKMKFGHHGANHPVQD
CCCCCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCEEEEEEECCCCCCCCHHH
LGTGKVIITSQNHGFAVDTATLPANARVTHLSLFDGSLQGFELAGKPVFCFQGHPEASPG
CCCCEEEEEECCCCEEEEEEECCCCCEEEEEEEECCCCCCEEECCCEEEEECCCCCCCCC
PHDLDYLFDKFAGLMERKRTKAI
CCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PQHSYAVLALADGTVFRGMSIGAEGISTGEVVFNTSMTGYQEILTDPSYCRQIVTLTYP
CCCCEEEEEEECCCEECCCCCCCCCCCCCCEEEECCCCHHHHHHCCHHHHHHHHHEECC
HIGNTGTNPDDVESGNIDRVYAAGLVIRDLPLASSNWRETQTLPEYLRQQGVVAIADIDT
CCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEECCH
RKLTRILREKGAQAGCIIAGQPDETEALKLAREFPGLAGMDLAKVVSCLQPYEWNEGEWR
HHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCEE
LGQGYPVQSHPRFHVAAMDFGIKRNILRKLAQRGCQITVFPAQTTADEILASQPDGIFLS
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCEEEE
NGPGDPEPCDYAIETTRRLLEKEIPVFGVCMGHQLLGLATGARTIKMKFGHHGANHPVQD
CCCCCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCEEEEEEECCCCCCCCHHH
LGTGKVIITSQNHGFAVDTATLPANARVTHLSLFDGSLQGFELAGKPVFCFQGHPEASPG
CCCCEEEEEECCCCEEEEEEECCCCCEEEEEEEECCCCCCEEECCCEEEEECCCCCCCCC
PHDLDYLFDKFAGLMERKRTKAI
CCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]