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The map label for this gene is topB [H]

Identifier: 82701530

GI number: 82701530

Start: 437968

End: 440508

Strand: Direct

Name: topB [H]

Synonym: Nmul_A0396

Alternate gene names: 82701530

Gene position: 437968-440508 (Clockwise)

Preceding gene: 82701529

Following gene: 82701534

Centisome position: 13.75

GC content: 53.68

Gene sequence:

>2541_bases
ATGGGTAAAACCTTAATCATTGCCGAGAAGCCTTCTGTTGCCGCAGACATTGCGCGTGCCTTAGGGGGATTCACGAAACA
TACCGACTACTTCGAGAACGATGAATATGTCGTGTCGTCCGCCGTAGGGCATTTGCTTGAGCTCGCGGTCCCTCCGGAAC
ACGAAGTCAAGCGAGGGAAATGGAGTTTTACGAATCTGCCCGTCATTCCACCGCATTTTGACCTCAATCCCATCGAGAAA
ACCGAATCGCGGCTGAAGCTTTTGACCAGACTCATCAAACGGAAGGATGTGGACGCGCTGATCAATGCTTGCGATGCAGG
CCGGGAAGGGGAACTGATTTTTCGCTACATCACTCGCCATGTCGGAACCAAAAAGCCGGTTAAACGGCTATGGCTGCAAT
CGATGACGCCCGCTTCCATCCGGGAGGGTTTCAATAAGTTGCTGGATGATGAGGACGTGTGGCCATTGGCGGAAGCAGCG
GTAAGCCGTTCAGAAGCTGACTGGCTGGTGGGCATAAACGGCACACGCGCCATGACCGCATTCAATTCACAGGAAGGCGG
CTTCCATAAAACCACAGTGGGACGTGTACAAACTCCTACATTGGCGATTCTGATCGAGCGCGAAGACATCATCAAGAAAT
TTGTCCCTCGCAGCTACTGGGAAGTGCATGCAACGTTTTCCGCCCGCGAAGGAAGCTATAAGGGTCGCTGGTTCGACGAG
TCGTTCACGAAAGCCAGGAAAGACAATGAGCAAAAAGCAGAGCGGTTATGGGACCATGCGACTGCGGAAGCTATTCGTGT
CAAGTGCCTCGGTAAATCTGGGGTCGTCACCGAAGAAAGCAAGCCTGCTACCGAAACCTGCCCTTTGCTCTATGACCTTA
CCAGTTTGCAGCGCGATGCGAACGGCCGTTTCGGCTTTTCCGCCAAGACTACCCTCTCCCTGGCGCAGGCATTATATGAA
AAGCATAAGGTATTGACTTATCCGCGCACGGACTCGCGGGCTCTGCCGGAAGATTATATTGCCACCGTCAAGGAAACCCT
GGGTTCTCTCCAAAACTCTGGATACGGAGCATTTGCTGCGCAGATTCTCGGAGCAGGCTGGGTTAAGCCCAATAAACGTA
TATTCAACAATGCAAAAATCTCCGACCACTTTGCGATCATCCCTACGACCATTGAAGCCAGGGGGTTGAACGAGGTGGAG
TCAAAACTGTACGATCTTGTTGTCAAGCGTTTCCTCGCGATATTCTACCCGGCGGCTGAATTTCTCGTTACCACCCGTAT
CACGCGGGTGGAGAACGAGCCTTTCAAGACGGAAGGAAAAGTTCTGATCAATCCCGGCTGGCAGGCCGTTTACGGCAAGG
AGGCGCAGCCTTCGGGTACGGAGGACAACTCGACCCTGGTCAAGCTGGAGCCGGATGAACCGGTGACCGCCGAGGAAATT
GAGGTTGCCTCGCATCAGACTCGCCCCCCTCCCCGATTCAATGAAGCCACTCTGCTGTCCGCGATGGAAGGCGCGGGTAA
ATGGGTGGAAGATGAAGAACTGCGGGAAGCCATGAGCGCAAAGGGACTTGGCACGCCGGCGACACGCGCGGCGATTATCG
AAGGCCTGGTATCGGAAAACTACATCCAGCGTGTGGGACGGGAACTGCATCCCACTGCCAAGGCCTTTTCCCTCATCACC
TTGTTGCGCGGGTTGAAAGTACCGGAACTTACTTCGCCTGAGCTGACGGGTGACTGGGAATACAAGCTGCGCCAGATCGA
ACAGGGGAAGCTCGAACGCAACAAGTTCATGGCTGAAATCGCGGAGATGACGAGCCACATCGTGCAACAGGCAAAAAGTC
ATCGCGGCGAAACCATAAGTGGAGATTTTTCGACGCTCAAGTCGCCCTGTCCGAAATGCGGCGGTGTGATCCAGGAGACA
TATAAAAAATTTCAGTGCCAGAAATGCGATTTCGCGCTATGGAAAATTCTGGCGGGACGACAATTTGAAACAGCGGAAAT
GGAGCAGCTCATTACCCGGCGGGAGGTGGGGCCTCTCCAGGGCTTTCGGAGCAAGATGGGACGGCTGTTCAACGCCAACA
TCAAACTGACGGATGAGCTCGAGATGAAGTTTGATTTCGGTGCAGACGGGGAGCAGGATACTGAAGAGGTTGATTTTTCC
GGGCAGGAACCGTTGGGAAAGTGTCCCCGGTGCAGTGGGCGGGTATTCGATCATGGCATGAGCTACCTGTGCGAAAAATC
GCTCGGTTCTGCACACACATGCAGCTTTAAAATAGGGAAAATCATTCTCTCGCGGCCGCTCGAGCGCGAGCAGGTCATGA
AATTGTTGCAGACCGGCAAAACGGATTTGCTGACAAAGTTTATTTCGAAGAAGGGCAGGCCGTTTTCAGCCTATCTCGTT
GCGGGCAAGGACGGAAAAATCGGGTTCGAGTTCGAAGTGAAGGAGGCAAAGGTTAAAACCCCGGCAGAGACGGAGGAAAC
AAAGGTGGCGAAGCCGAGGGCTGCGCCGATGAAAAAAACCCTCTCCGCGAAAACGAGGTAA

Upstream 100 bases:

>100_bases
ATGTTTTTGGTTGCAGGTCGCTGGCAGTGCTATCTATTTTTGCTTGCCCCCTGTTATGCTGATAAATCACACGGGGTGGT
GGGACATTTACAGGAATTTC

Downstream 100 bases:

>100_bases
AAAGGGCAGTGGAGCGCAGCCTGCTTCTTGAAGAAGACCCGTTCGGGAGATCGGGGGACTGCGAGAATGGGAGAAGAGGG
AAAGGGCGTGATTGCGTAAC

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 846; Mature: 845

Protein sequence:

>846_residues
MGKTLIIAEKPSVAADIARALGGFTKHTDYFENDEYVVSSAVGHLLELAVPPEHEVKRGKWSFTNLPVIPPHFDLNPIEK
TESRLKLLTRLIKRKDVDALINACDAGREGELIFRYITRHVGTKKPVKRLWLQSMTPASIREGFNKLLDDEDVWPLAEAA
VSRSEADWLVGINGTRAMTAFNSQEGGFHKTTVGRVQTPTLAILIEREDIIKKFVPRSYWEVHATFSAREGSYKGRWFDE
SFTKARKDNEQKAERLWDHATAEAIRVKCLGKSGVVTEESKPATETCPLLYDLTSLQRDANGRFGFSAKTTLSLAQALYE
KHKVLTYPRTDSRALPEDYIATVKETLGSLQNSGYGAFAAQILGAGWVKPNKRIFNNAKISDHFAIIPTTIEARGLNEVE
SKLYDLVVKRFLAIFYPAAEFLVTTRITRVENEPFKTEGKVLINPGWQAVYGKEAQPSGTEDNSTLVKLEPDEPVTAEEI
EVASHQTRPPPRFNEATLLSAMEGAGKWVEDEELREAMSAKGLGTPATRAAIIEGLVSENYIQRVGRELHPTAKAFSLIT
LLRGLKVPELTSPELTGDWEYKLRQIEQGKLERNKFMAEIAEMTSHIVQQAKSHRGETISGDFSTLKSPCPKCGGVIQET
YKKFQCQKCDFALWKILAGRQFETAEMEQLITRREVGPLQGFRSKMGRLFNANIKLTDELEMKFDFGADGEQDTEEVDFS
GQEPLGKCPRCSGRVFDHGMSYLCEKSLGSAHTCSFKIGKIILSRPLEREQVMKLLQTGKTDLLTKFISKKGRPFSAYLV
AGKDGKIGFEFEVKEAKVKTPAETEETKVAKPRAAPMKKTLSAKTR

Sequences:

>Translated_846_residues
MGKTLIIAEKPSVAADIARALGGFTKHTDYFENDEYVVSSAVGHLLELAVPPEHEVKRGKWSFTNLPVIPPHFDLNPIEK
TESRLKLLTRLIKRKDVDALINACDAGREGELIFRYITRHVGTKKPVKRLWLQSMTPASIREGFNKLLDDEDVWPLAEAA
VSRSEADWLVGINGTRAMTAFNSQEGGFHKTTVGRVQTPTLAILIEREDIIKKFVPRSYWEVHATFSAREGSYKGRWFDE
SFTKARKDNEQKAERLWDHATAEAIRVKCLGKSGVVTEESKPATETCPLLYDLTSLQRDANGRFGFSAKTTLSLAQALYE
KHKVLTYPRTDSRALPEDYIATVKETLGSLQNSGYGAFAAQILGAGWVKPNKRIFNNAKISDHFAIIPTTIEARGLNEVE
SKLYDLVVKRFLAIFYPAAEFLVTTRITRVENEPFKTEGKVLINPGWQAVYGKEAQPSGTEDNSTLVKLEPDEPVTAEEI
EVASHQTRPPPRFNEATLLSAMEGAGKWVEDEELREAMSAKGLGTPATRAAIIEGLVSENYIQRVGRELHPTAKAFSLIT
LLRGLKVPELTSPELTGDWEYKLRQIEQGKLERNKFMAEIAEMTSHIVQQAKSHRGETISGDFSTLKSPCPKCGGVIQET
YKKFQCQKCDFALWKILAGRQFETAEMEQLITRREVGPLQGFRSKMGRLFNANIKLTDELEMKFDFGADGEQDTEEVDFS
GQEPLGKCPRCSGRVFDHGMSYLCEKSLGSAHTCSFKIGKIILSRPLEREQVMKLLQTGKTDLLTKFISKKGRPFSAYLV
AGKDGKIGFEFEVKEAKVKTPAETEETKVAKPRAAPMKKTLSAKTR
>Mature_845_residues
GKTLIIAEKPSVAADIARALGGFTKHTDYFENDEYVVSSAVGHLLELAVPPEHEVKRGKWSFTNLPVIPPHFDLNPIEKT
ESRLKLLTRLIKRKDVDALINACDAGREGELIFRYITRHVGTKKPVKRLWLQSMTPASIREGFNKLLDDEDVWPLAEAAV
SRSEADWLVGINGTRAMTAFNSQEGGFHKTTVGRVQTPTLAILIEREDIIKKFVPRSYWEVHATFSAREGSYKGRWFDES
FTKARKDNEQKAERLWDHATAEAIRVKCLGKSGVVTEESKPATETCPLLYDLTSLQRDANGRFGFSAKTTLSLAQALYEK
HKVLTYPRTDSRALPEDYIATVKETLGSLQNSGYGAFAAQILGAGWVKPNKRIFNNAKISDHFAIIPTTIEARGLNEVES
KLYDLVVKRFLAIFYPAAEFLVTTRITRVENEPFKTEGKVLINPGWQAVYGKEAQPSGTEDNSTLVKLEPDEPVTAEEIE
VASHQTRPPPRFNEATLLSAMEGAGKWVEDEELREAMSAKGLGTPATRAAIIEGLVSENYIQRVGRELHPTAKAFSLITL
LRGLKVPELTSPELTGDWEYKLRQIEQGKLERNKFMAEIAEMTSHIVQQAKSHRGETISGDFSTLKSPCPKCGGVIQETY
KKFQCQKCDFALWKILAGRQFETAEMEQLITRREVGPLQGFRSKMGRLFNANIKLTDELEMKFDFGADGEQDTEEVDFSG
QEPLGKCPRCSGRVFDHGMSYLCEKSLGSAHTCSFKIGKIILSRPLEREQVMKLLQTGKTDLLTKFISKKGRPFSAYLVA
GKDGKIGFEFEVKEAKVKTPAETEETKVAKPRAAPMKKTLSAKTR

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=702, Percent_Identity=24.0740740740741, Blast_Score=133, Evalue=7e-31,
Organism=Homo sapiens, GI10835218, Length=623, Percent_Identity=24.7191011235955, Blast_Score=124, Evalue=5e-28,
Organism=Escherichia coli, GI1788061, Length=621, Percent_Identity=31.7230273752013, Blast_Score=306, Evalue=4e-84,
Organism=Escherichia coli, GI1787529, Length=670, Percent_Identity=26.2686567164179, Blast_Score=148, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI32563869, Length=644, Percent_Identity=25.3105590062112, Blast_Score=135, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17555378, Length=651, Percent_Identity=24.7311827956989, Blast_Score=106, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6323263, Length=679, Percent_Identity=23.4167893961708, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24640096, Length=704, Percent_Identity=23.7215909090909, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24585251, Length=695, Percent_Identity=23.7410071942446, Blast_Score=107, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 94638; Mature: 94507

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTLIIAEKPSVAADIARALGGFTKHTDYFENDEYVVSSAVGHLLELAVPPEHEVKRGK
CCCEEEEECCCCHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCCC
WSFTNLPVIPPHFDLNPIEKTESRLKLLTRLIKRKDVDALINACDAGREGELIFRYITRH
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHH
VGTKKPVKRLWLQSMTPASIREGFNKLLDDEDVWPLAEAAVSRSEADWLVGINGTRAMTA
CCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEECCCCEEEEE
FNSQEGGFHKTTVGRVQTPTLAILIEREDIIKKFVPRSYWEVHATFSAREGSYKGRWFDE
ECCCCCCCEECCCCCCCCCEEEEEECHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCH
SFTKARKDNEQKAERLWDHATAEAIRVKCLGKSGVVTEESKPATETCPLLYDLTSLQRDA
HHHHHHCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCHHCHHHHHHHHHHHCC
NGRFGFSAKTTLSLAQALYEKHKVLTYPRTDSRALPEDYIATVKETLGSLQNSGYGAFAA
CCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
QILGAGWVKPNKRIFNNAKISDHFAIIPTTIEARGLNEVESKLYDLVVKRFLAIFYPAAE
HHHHCCCCCCCHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FLVTTRITRVENEPFKTEGKVLINPGWQAVYGKEAQPSGTEDNSTLVKLEPDEPVTAEEI
HHHHHHHHHHCCCCCCCCCEEEECCCCCEECCCCCCCCCCCCCCEEEEECCCCCCCHHHH
EVASHQTRPPPRFNEATLLSAMEGAGKWVEDEELREAMSAKGLGTPATRAAIIEGLVSEN
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
YIQRVGRELHPTAKAFSLITLLRGLKVPELTSPELTGDWEYKLRQIEQGKLERNKFMAEI
HHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AEMTSHIVQQAKSHRGETISGDFSTLKSPCPKCGGVIQETYKKFQCQKCDFALWKILAGR
HHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCC
QFETAEMEQLITRREVGPLQGFRSKMGRLFNANIKLTDELEMKFDFGADGEQDTEEVDFS
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECEEEEEECCCCCCCCCHHCCCC
GQEPLGKCPRCSGRVFDHGMSYLCEKSLGSAHTCSFKIGKIILSRPLEREQVMKLLQTGK
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHCCCCHHHHHHHHHCCC
TDLLTKFISKKGRPFSAYLVAGKDGKIGFEFEVKEAKVKTPAETEETKVAKPRAAPMKKT
HHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHCCCCCCCHHHHH
LSAKTR
HHCCCC
>Mature Secondary Structure 
GKTLIIAEKPSVAADIARALGGFTKHTDYFENDEYVVSSAVGHLLELAVPPEHEVKRGK
CCEEEEECCCCHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCCC
WSFTNLPVIPPHFDLNPIEKTESRLKLLTRLIKRKDVDALINACDAGREGELIFRYITRH
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHH
VGTKKPVKRLWLQSMTPASIREGFNKLLDDEDVWPLAEAAVSRSEADWLVGINGTRAMTA
CCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEECCCCEEEEE
FNSQEGGFHKTTVGRVQTPTLAILIEREDIIKKFVPRSYWEVHATFSAREGSYKGRWFDE
ECCCCCCCEECCCCCCCCCEEEEEECHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCH
SFTKARKDNEQKAERLWDHATAEAIRVKCLGKSGVVTEESKPATETCPLLYDLTSLQRDA
HHHHHHCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCHHCHHHHHHHHHHHCC
NGRFGFSAKTTLSLAQALYEKHKVLTYPRTDSRALPEDYIATVKETLGSLQNSGYGAFAA
CCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
QILGAGWVKPNKRIFNNAKISDHFAIIPTTIEARGLNEVESKLYDLVVKRFLAIFYPAAE
HHHHCCCCCCCHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FLVTTRITRVENEPFKTEGKVLINPGWQAVYGKEAQPSGTEDNSTLVKLEPDEPVTAEEI
HHHHHHHHHHCCCCCCCCCEEEECCCCCEECCCCCCCCCCCCCCEEEEECCCCCCCHHHH
EVASHQTRPPPRFNEATLLSAMEGAGKWVEDEELREAMSAKGLGTPATRAAIIEGLVSEN
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
YIQRVGRELHPTAKAFSLITLLRGLKVPELTSPELTGDWEYKLRQIEQGKLERNKFMAEI
HHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AEMTSHIVQQAKSHRGETISGDFSTLKSPCPKCGGVIQETYKKFQCQKCDFALWKILAGR
HHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCC
QFETAEMEQLITRREVGPLQGFRSKMGRLFNANIKLTDELEMKFDFGADGEQDTEEVDFS
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECEEEEEECCCCCCCCCHHCCCC
GQEPLGKCPRCSGRVFDHGMSYLCEKSLGSAHTCSFKIGKIILSRPLEREQVMKLLQTGK
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHCCCCHHHHHHHHHCCC
TDLLTKFISKKGRPFSAYLVAGKDGKIGFEFEVKEAKVKTPAETEETKVAKPRAAPMKKT
HHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHCCCCCCCHHHHH
LSAKTR
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA