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The map label for this gene is cheB

Identifier: 82701468

GI number: 82701468

Start: 363611

End: 364678

Strand: Direct

Name: cheB

Synonym: Nmul_A0334

Alternate gene names: 82701468

Gene position: 363611-364678 (Clockwise)

Preceding gene: 82701467

Following gene: 82701469

Centisome position: 11.42

GC content: 54.59

Gene sequence:

>1068_bases
ATGATAAACGTCCTGGTAGTGGAAGATTCACCGGTAGTGCGAGAGTTTCTTATTTACATACTCAATTCCGATCCTGATAT
CACTGTTATTGCGACCGCCGGCGACGGAGAAGAAGCAATCGATGCCTTGCGCAACCATCGGCCTGATGTAATCACAATGG
ATATTCACATGCCGAAGCTGAATGGAGTGGAGGCCACACAGCTGATCATGGAGACACAGCCGACTCCCATTGTCATCGTG
AGCGGCAGCACGGATCCGGCAGAGGTTGGGACTACATTTGATGCGATCGATGCAGGGGCATTGGCAGTGCTCCCGCGCCC
GGCAGGCATCGGCCATCCGGATCACGAGGCGACCGCTCGGAAGATGATAGAGGTCGTAAAACTGATGTCGGAAGTAAAGG
TAGTCCGTCGCTGGGCGCGCATGCGAGGGGCTTCTCCGGCCTCACATTCTGTACGCGCGCCCCTGCTTAACCGTGTGGCG
CCAGCAAGAATTGTCGTTGTGGGTGCTTCAACGGGTGGCCCGCCTGCACTCGAGGCGATTCTTTCATCACTCCCAAAGCA
TTTTCCTGTGCCCATTCTAATCGTTCAGCATATGACTACGGGATTCATGGAAGGATTTGTCCAATGGCTGAAGAACTCGT
CCAATCTTCCCGTTCATATTGCAAGGCAGGGTGAGTTGCCCCTGCCAGGGCATGTTTATATGGCACCCGATGAATACCAG
ATGAAGGTGGAGAATCGAGGGGAGATCGTTTTGACCAAGGACGAGCCAGAGCATGGTTCAAGACCCTCAATTTCATATCT
TTTCAGGGCGGTTGCCAGAGTCTACGGTCACGATGCCGTTGCTGGCCTATTGACGGGGATGGGCCGTGATGGTGCCGACG
AGTTACGCCTTCTGAAGGAGAAAGGCGCGATTACCTTCGCACAAGACAAGGAAAGTTCGGTCGTCCACGGTATGGCGGGC
GAGGCGATCAGGTTAGGTGCGGCGACCATGGTCTTGCCTCTGGAAAAGATTGCCGCAGCGCTAACGAATCTGGTGGCCGA
ACGAGGAGAAACTAAGCTTGGCGCATGA

Upstream 100 bases:

>100_bases
AATGCATATATCGTGAAGAGTCGCTTTGACCAAGGCAACCTGCTGGAAGCGATCCGACGCCTGATCAGCATCAGCAGCAT
TAATCCGTGAGTTTCCTCCC

Downstream 100 bases:

>100_bases
TATTTCAATCCACGATGCCCGGAAAGTCGAGATTCTCATCGCAGAGGACAGTCCCACACAAGCGGAAAAACTGAAGTATC
TGCTGGAGGAAAACGGCTAC

Product: response regulator receiver (CheY-like) modulated CheB methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MINVLVVEDSPVVREFLIYILNSDPDITVIATAGDGEEAIDALRNHRPDVITMDIHMPKLNGVEATQLIMETQPTPIVIV
SGSTDPAEVGTTFDAIDAGALAVLPRPAGIGHPDHEATARKMIEVVKLMSEVKVVRRWARMRGASPASHSVRAPLLNRVA
PARIVVVGASTGGPPALEAILSSLPKHFPVPILIVQHMTTGFMEGFVQWLKNSSNLPVHIARQGELPLPGHVYMAPDEYQ
MKVENRGEIVLTKDEPEHGSRPSISYLFRAVARVYGHDAVAGLLTGMGRDGADELRLLKEKGAITFAQDKESSVVHGMAG
EAIRLGAATMVLPLEKIAAALTNLVAERGETKLGA

Sequences:

>Translated_355_residues
MINVLVVEDSPVVREFLIYILNSDPDITVIATAGDGEEAIDALRNHRPDVITMDIHMPKLNGVEATQLIMETQPTPIVIV
SGSTDPAEVGTTFDAIDAGALAVLPRPAGIGHPDHEATARKMIEVVKLMSEVKVVRRWARMRGASPASHSVRAPLLNRVA
PARIVVVGASTGGPPALEAILSSLPKHFPVPILIVQHMTTGFMEGFVQWLKNSSNLPVHIARQGELPLPGHVYMAPDEYQ
MKVENRGEIVLTKDEPEHGSRPSISYLFRAVARVYGHDAVAGLLTGMGRDGADELRLLKEKGAITFAQDKESSVVHGMAG
EAIRLGAATMVLPLEKIAAALTNLVAERGETKLGA
>Mature_355_residues
MINVLVVEDSPVVREFLIYILNSDPDITVIATAGDGEEAIDALRNHRPDVITMDIHMPKLNGVEATQLIMETQPTPIVIV
SGSTDPAEVGTTFDAIDAGALAVLPRPAGIGHPDHEATARKMIEVVKLMSEVKVVRRWARMRGASPASHSVRAPLLNRVA
PARIVVVGASTGGPPALEAILSSLPKHFPVPILIVQHMTTGFMEGFVQWLKNSSNLPVHIARQGELPLPGHVYMAPDEYQ
MKVENRGEIVLTKDEPEHGSRPSISYLFRAVARVYGHDAVAGLLTGMGRDGADELRLLKEKGAITFAQDKESSVVHGMAG
EAIRLGAATMVLPLEKIAAALTNLVAERGETKLGA

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=349, Percent_Identity=34.9570200573066, Blast_Score=177, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB_NITMU (Q2YC79)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411034.1
- ProteinModelPortal:   Q2YC79
- SMR:   Q2YC79
- STRING:   Q2YC79
- GeneID:   3785959
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0334
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- OMA:   RAIMSTS
- PhylomeDB:   Q2YC79
- ProtClustDB:   CLSK755255
- BioCyc:   NMUL323848:NMUL_A0334-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 38094; Mature: 38094

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 170-170 ACT_SITE 197-197 ACT_SITE 290-290

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINVLVVEDSPVVREFLIYILNSDPDITVIATAGDGEEAIDALRNHRPDVITMDIHMPKL
CEEEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEEECCCC
NGVEATQLIMETQPTPIVIVSGSTDPAEVGTTFDAIDAGALAVLPRPAGIGHPDHEATAR
CCCHHHHHHHCCCCCCEEEEECCCCHHHHCCCHHHCCCCCEEEECCCCCCCCCCHHHHHH
KMIEVVKLMSEVKVVRRWARMRGASPASHSVRAPLLNRVAPARIVVVGASTGGPPALEAI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCHHHHHH
LSSLPKHFPVPILIVQHMTTGFMEGFVQWLKNSSNLPVHIARQGELPLPGHVYMAPDEYQ
HHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEECCCHHE
MKVENRGEIVLTKDEPEHGSRPSISYLFRAVARVYGHDAVAGLLTGMGRDGADELRLLKE
EEECCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHH
KGAITFAQDKESSVVHGMAGEAIRLGAATMVLPLEKIAAALTNLVAERGETKLGA
CCCEEEECCCCCCCHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MINVLVVEDSPVVREFLIYILNSDPDITVIATAGDGEEAIDALRNHRPDVITMDIHMPKL
CEEEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEEECCCC
NGVEATQLIMETQPTPIVIVSGSTDPAEVGTTFDAIDAGALAVLPRPAGIGHPDHEATAR
CCCHHHHHHHCCCCCCEEEEECCCCHHHHCCCHHHCCCCCEEEECCCCCCCCCCHHHHHH
KMIEVVKLMSEVKVVRRWARMRGASPASHSVRAPLLNRVAPARIVVVGASTGGPPALEAI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCHHHHHH
LSSLPKHFPVPILIVQHMTTGFMEGFVQWLKNSSNLPVHIARQGELPLPGHVYMAPDEYQ
HHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEECCCHHE
MKVENRGEIVLTKDEPEHGSRPSISYLFRAVARVYGHDAVAGLLTGMGRDGADELRLLKE
EEECCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHH
KGAITFAQDKESSVVHGMAGEAIRLGAATMVLPLEKIAAALTNLVAERGETKLGA
CCCEEEECCCCCCCHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA