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The map label for this gene is wcaJ [H]

Identifier: 82701373

GI number: 82701373

Start: 255239

End: 256657

Strand: Direct

Name: wcaJ [H]

Synonym: Nmul_A0239

Alternate gene names: 82701373

Gene position: 255239-256657 (Clockwise)

Preceding gene: 82701372

Following gene: 82701374

Centisome position: 8.02

GC content: 52.57

Gene sequence:

>1419_bases
ATGGCTGAGGCAAATCCTACATTTTCCGCTTCAACCGTTACCGCCCGCCAGGTGAAAGGCCCGCTGGCTAATAATATGCT
GAGCGCGGTCGAGTCGGTACTTGATCCGGCAGTACTGACACTTTCGTTATGGCTGATAAGCACCAGTTTTGAAGGCGAAT
TGCTCCCGCCTTATTTGATCCTGTCGGTGATCGTATTCTCTATCTCCTTTCCAGGTGCGTCACATCTCCGGTCATCCGTC
AAGGTTCTCATCTTCGACGTGCTCTACACCTGGTTCTGGATGGCTTTGCTACTGTTTTTTCTCGGTTTTGCGACCGGCTA
TATCGCCCAATTCTCCAGCCAGGTGCTCATTACATGGCTATGGGCTGCCCCCCTAAGTCAAATCGGCGTCCATCTGCTGT
TTCGTATCGCCGCACCCTATCTTCTCATGCTCCAGGGTCCGCCACAGCGCGCCATCATCGTCGGCATGAACGAGCAGGGG
GTCGCCCTCGCCCGTCGTATTCATGAGACGCGTTATTCGAATATGGAACTGTCCGGTTTTTTTGATGATCGGAATGAGAG
CCGACTTTCGCATGCGCGGAATAATCCCTTGCTCGGAAGGCTTCGAGAGCTTCCCGAATTTGTCAAGGAGCATCGCATCC
AGTTCATTTATCTGTCGTTGCCGATGGCTTCCCAGCCACGCATCCTGCATGTTCTCGAAGAACTGAAAGATACGACGGCC
TCCATCTATTTCGTACCCGACATGTTTATTACGGATCTTATTCAGGCGCGAAGTGGCACAGTGTGTGGTACGCCGGTTAT
CGCTGTCTGCGAATCGCCTTTTACGGGCTCCAATGGGATGGTCAAGCGCGCAAGCGACATTATCCTCTCCCTGCTTATAC
TGCTACTCATATCACCACTTCTGCTGCTCATCGCGGTTGCCATCCGGCTGGATTCGCCAGGGCCCATTATTTTCAAGCAG
CGACGCTATGGGCTGGACGGAGAGGAAATCCTTGTTTACAAGTTTAGATCGATGAGCGTCTGCGAAGATGGGAATACCAT
CCGGCAAGCACAAAGGAATGATAACCGCATAACCCGCGTCGGGGCTTTCCTGCGAAGCAATTCCCTGGATGAATTGCCGC
AGTTCATCAATGTATTGCAGGGGCGCATGAGCATCGTGGGCCCCAGGCCTCATGCAGTAGCGCACAACGAGATATATCGC
AACCTTATAAAAGGCTACATGATCCGGCACAAGGTAAAGCCGGGAATTACAGGTTGGGCGCAGGTGAACGGCTACCGTGG
GGAGACGCGGACCCTGGACAAGATGCAGGCGCGTATCGATCATGATCTCGATTATTTACGCAACTGGTCGTTGCGGCTCG
ATTTGCACATCATCTGCAAGACTATCCTGGTAGTACTGAAAGATCGGGCTGCATATTAA

Upstream 100 bases:

>100_bases
AGTTCTTACACTGTCAAAAACCACCTGCACCAGATATTCCGGAAACTCGATGTCTACAATCGGGTGCAAGCCATTTCCAA
AATTGAACAGCGTGTTGGTC

Downstream 100 bases:

>100_bases
ATGCTCTCTCATGCGCCGTAAAGCCTCCCAGCAGTCATTAACACGAATAACACTTCCGCTATCCTCTCGTTAATCAAGGT
GATACTCTTGCAGTCTCCTT

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MAEANPTFSASTVTARQVKGPLANNMLSAVESVLDPAVLTLSLWLISTSFEGELLPPYLILSVIVFSISFPGASHLRSSV
KVLIFDVLYTWFWMALLLFFLGFATGYIAQFSSQVLITWLWAAPLSQIGVHLLFRIAAPYLLMLQGPPQRAIIVGMNEQG
VALARRIHETRYSNMELSGFFDDRNESRLSHARNNPLLGRLRELPEFVKEHRIQFIYLSLPMASQPRILHVLEELKDTTA
SIYFVPDMFITDLIQARSGTVCGTPVIAVCESPFTGSNGMVKRASDIILSLLILLLISPLLLLIAVAIRLDSPGPIIFKQ
RRYGLDGEEILVYKFRSMSVCEDGNTIRQAQRNDNRITRVGAFLRSNSLDELPQFINVLQGRMSIVGPRPHAVAHNEIYR
NLIKGYMIRHKVKPGITGWAQVNGYRGETRTLDKMQARIDHDLDYLRNWSLRLDLHIICKTILVVLKDRAAY

Sequences:

>Translated_472_residues
MAEANPTFSASTVTARQVKGPLANNMLSAVESVLDPAVLTLSLWLISTSFEGELLPPYLILSVIVFSISFPGASHLRSSV
KVLIFDVLYTWFWMALLLFFLGFATGYIAQFSSQVLITWLWAAPLSQIGVHLLFRIAAPYLLMLQGPPQRAIIVGMNEQG
VALARRIHETRYSNMELSGFFDDRNESRLSHARNNPLLGRLRELPEFVKEHRIQFIYLSLPMASQPRILHVLEELKDTTA
SIYFVPDMFITDLIQARSGTVCGTPVIAVCESPFTGSNGMVKRASDIILSLLILLLISPLLLLIAVAIRLDSPGPIIFKQ
RRYGLDGEEILVYKFRSMSVCEDGNTIRQAQRNDNRITRVGAFLRSNSLDELPQFINVLQGRMSIVGPRPHAVAHNEIYR
NLIKGYMIRHKVKPGITGWAQVNGYRGETRTLDKMQARIDHDLDYLRNWSLRLDLHIICKTILVVLKDRAAY
>Mature_471_residues
AEANPTFSASTVTARQVKGPLANNMLSAVESVLDPAVLTLSLWLISTSFEGELLPPYLILSVIVFSISFPGASHLRSSVK
VLIFDVLYTWFWMALLLFFLGFATGYIAQFSSQVLITWLWAAPLSQIGVHLLFRIAAPYLLMLQGPPQRAIIVGMNEQGV
ALARRIHETRYSNMELSGFFDDRNESRLSHARNNPLLGRLRELPEFVKEHRIQFIYLSLPMASQPRILHVLEELKDTTAS
IYFVPDMFITDLIQARSGTVCGTPVIAVCESPFTGSNGMVKRASDIILSLLILLLISPLLLLIAVAIRLDSPGPIIFKQR
RYGLDGEEILVYKFRSMSVCEDGNTIRQAQRNDNRITRVGAFLRSNSLDELPQFINVLQGRMSIVGPRPHAVAHNEIYRN
LIKGYMIRHKVKPGITGWAQVNGYRGETRTLDKMQARIDHDLDYLRNWSLRLDLHIICKTILVVLKDRAAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=456, Percent_Identity=40.3508771929825, Blast_Score=330, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 53205; Mature: 53074

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEANPTFSASTVTARQVKGPLANNMLSAVESVLDPAVLTLSLWLISTSFEGELLPPYLI
CCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHEECCCCCCCCHHHH
LSVIVFSISFPGASHLRSSVKVLIFDVLYTWFWMALLLFFLGFATGYIAQFSSQVLITWL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
WAAPLSQIGVHLLFRIAAPYLLMLQGPPQRAIIVGMNEQGVALARRIHETRYSNMELSGF
HHHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEE
FDDRNESRLSHARNNPLLGRLRELPEFVKEHRIQFIYLSLPMASQPRILHVLEELKDTTA
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHCCE
SIYFVPDMFITDLIQARSGTVCGTPVIAVCESPFTGSNGMVKRASDIILSLLILLLISPL
EEEEECHHHHHHHHHHCCCCCCCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLLIAVAIRLDSPGPIIFKQRRYGLDGEEILVYKFRSMSVCEDGNTIRQAQRNDNRITRV
HHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHCCCHHHHH
GAFLRSNSLDELPQFINVLQGRMSIVGPRPHAVAHNEIYRNLIKGYMIRHKVKPGITGWA
HHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
QVNGYRGETRTLDKMQARIDHDLDYLRNWSLRLDLHIICKTILVVLKDRAAY
EECCCCCCHHHHHHHHHHHHHCHHHHHCCCEEEHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AEANPTFSASTVTARQVKGPLANNMLSAVESVLDPAVLTLSLWLISTSFEGELLPPYLI
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHEECCCCCCCCHHHH
LSVIVFSISFPGASHLRSSVKVLIFDVLYTWFWMALLLFFLGFATGYIAQFSSQVLITWL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
WAAPLSQIGVHLLFRIAAPYLLMLQGPPQRAIIVGMNEQGVALARRIHETRYSNMELSGF
HHHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEE
FDDRNESRLSHARNNPLLGRLRELPEFVKEHRIQFIYLSLPMASQPRILHVLEELKDTTA
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHCCE
SIYFVPDMFITDLIQARSGTVCGTPVIAVCESPFTGSNGMVKRASDIILSLLILLLISPL
EEEEECHHHHHHHHHHCCCCCCCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLLIAVAIRLDSPGPIIFKQRRYGLDGEEILVYKFRSMSVCEDGNTIRQAQRNDNRITRV
HHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHCCCHHHHH
GAFLRSNSLDELPQFINVLQGRMSIVGPRPHAVAHNEIYRNLIKGYMIRHKVKPGITGWA
HHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
QVNGYRGETRTLDKMQARIDHDLDYLRNWSLRLDLHIICKTILVVLKDRAAY
EECCCCCCHHHHHHHHHHHHHCHHHHHCCCEEEHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]