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The map label for this gene is phbI [H]

Identifier: 82701354

GI number: 82701354

Start: 233680

End: 235407

Strand: Direct

Name: phbI [H]

Synonym: Nmul_A0220

Alternate gene names: 82701354

Gene position: 233680-235407 (Clockwise)

Preceding gene: 82701353

Following gene: 82701355

Centisome position: 7.34

GC content: 56.13

Gene sequence:

>1728_bases
ATGAGCTTTGTACTGCATGGGGTGGGCGTTTCGGAAGGTATCGCCATAGGCCATGCTCATCTTGCGTCTCATGCCGCGCT
GGAAGTCGCCCACCACGTCGTGCCCGAGGATCAGGTCACCAATGAAATCTCCCGGCTGGACACCGCTTTCACAACCGTTC
GCAAAGAGCTTCAGGCATTGCACGACACGGTCGTCAGCGGTCCGGCGGCTGCCGAATACGAAGCATTTCTCGATCTGCAC
CGGATGATTCTGGATGATCCCACGCTCTCGACTGCGGCAAAAGCGTATATTGCCCAGAATCAGTGCAATGCCGAATGGGC
CATTACCCAGCAGATGGGGGTATTGATGGCGCAGTTCGAGGAAATAGAAGATCCCTATCTGCGCGAACGTAAAACGGACG
TCATCCAGGTGGTGGAGCGCGTTCTCAAGGTATTGCTGGGGCATCCCGGATATATTCCCCCTTCGCAAAAGCGGGATGGC
GACAGTGTCCTGGTAGCGCACGACTTGAGTCCGGCCGATGTGCTTCAATATAAGCAGTATTCGTTTACTGCATTCCTGAC
GGATCTTGGCGGCCTGACTTCGCATACCGCCATTGTCGCCCGCAGCCTGAACATCCCCTCCGTCGTCGCGCTTCACCATG
CCCGCCGCCTCATCCGGGAAAATGACATTCTGATCGTGGATGGCAACCAGGGTGTGGTCATCGTGGACCCGGACGAGCAT
GTGCTCGCGGAATATCGGCTGCGCCAGAGCGAGCTGGAGCTGGAAAAACAGAAACTCAAGCGCTTGAGGACGACGGTCGC
GGCGACGCTGGATGGCACGGTAGTGGAACTGTATGCAAATATCGAGTTGCCGCAGGACGTCGATCAGGTCAAAGAAAATG
GAGCGACCGGCGTCGGCCTCTTTCGGAGCGAATTCCTGTTCCTCAATCGGGACAGCTTGCCTGATGAGGAAGAGCAATTC
GAAGCTTATCGAACCGTGGCGCGAAAAATGCGGGGAATGCCGGTAACGGTGCGCACGTTCGATCTTGGCGCGGACAAGAA
CCTGGATCATGCCAAACGGGTGGCTGCGAATCCCGCCCTGGGCCTGCGAGCCATCCGGCTGAGCCTGGCCGAACCCCAGA
TGTTCAATATCCAGTTGCGCGCTATTTTACGTGCTTCGCGCTATGGGCAGATCCGGATTCTGGTGCCGATGCTTTCCAAT
GTTGCTGAGATAACGCAGACGCTGCATTTGATCCAAAGCGCCAAACAAAGCCTGCGTAACGAAAAGATTCCTTTTGACGA
AAAAGTGCAGGTGGGAGGAATGATAGAAATTCCAGCCGCTGCGCTGAGTCTTGACATTTTCATGCGCAAGCTCGATTTCC
TGTCAATCGGCACCAATGATCTCATCCAGTATACGCTGGCGATAGACCGCGCCGATGACACTGTCGCGCACCTCTATGAT
TCGCTGCACCCGGCGGTGCTGCGGTTGGTCGCGCATGTCATACGCAGCGCGAACCGTGCAAGCATACCGGTGTCCGTGTG
CGGCGAGATCGCAGGGGATGTGGTGTTTACCCGTCTGCTGCTCGGTTTCGGCTTACGCGTGTTTTCCATGCACCCCGTCC
AACTGCTGACCGTAAAACGTGAAGTCTTGCGGGCAAATCTGCCCGACCTCATTCCGATCACTCAAAAAATACTGAAAACC
GCTGATCCGGAGAAAATTCACGCACTGCTGGCGAAGCTCAACGCTTGA

Upstream 100 bases:

>100_bases
ACCAGGGCGCGACCCTGATCATCGAAACTGCGGGTGAGGACGAGATTGAGGCAATGCAGGCCTTGGTACAGCTGATCGAG
GATCGCTTCGGAGAAAGCGA

Downstream 100 bases:

>100_bases
ACGATCGTTCCCTGTTCCATTTCACCGAATGCCGATGCATATGAGCAGCGTGTACGACTATAATTGCCGGTTACAATCAG
GCGATGCGATTATTTGAAAG

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I; Protein I [H]

Number of amino acids: Translated: 575; Mature: 574

Protein sequence:

>575_residues
MSFVLHGVGVSEGIAIGHAHLASHAALEVAHHVVPEDQVTNEISRLDTAFTTVRKELQALHDTVVSGPAAAEYEAFLDLH
RMILDDPTLSTAAKAYIAQNQCNAEWAITQQMGVLMAQFEEIEDPYLRERKTDVIQVVERVLKVLLGHPGYIPPSQKRDG
DSVLVAHDLSPADVLQYKQYSFTAFLTDLGGLTSHTAIVARSLNIPSVVALHHARRLIRENDILIVDGNQGVVIVDPDEH
VLAEYRLRQSELELEKQKLKRLRTTVAATLDGTVVELYANIELPQDVDQVKENGATGVGLFRSEFLFLNRDSLPDEEEQF
EAYRTVARKMRGMPVTVRTFDLGADKNLDHAKRVAANPALGLRAIRLSLAEPQMFNIQLRAILRASRYGQIRILVPMLSN
VAEITQTLHLIQSAKQSLRNEKIPFDEKVQVGGMIEIPAAALSLDIFMRKLDFLSIGTNDLIQYTLAIDRADDTVAHLYD
SLHPAVLRLVAHVIRSANRASIPVSVCGEIAGDVVFTRLLLGFGLRVFSMHPVQLLTVKREVLRANLPDLIPITQKILKT
ADPEKIHALLAKLNA

Sequences:

>Translated_575_residues
MSFVLHGVGVSEGIAIGHAHLASHAALEVAHHVVPEDQVTNEISRLDTAFTTVRKELQALHDTVVSGPAAAEYEAFLDLH
RMILDDPTLSTAAKAYIAQNQCNAEWAITQQMGVLMAQFEEIEDPYLRERKTDVIQVVERVLKVLLGHPGYIPPSQKRDG
DSVLVAHDLSPADVLQYKQYSFTAFLTDLGGLTSHTAIVARSLNIPSVVALHHARRLIRENDILIVDGNQGVVIVDPDEH
VLAEYRLRQSELELEKQKLKRLRTTVAATLDGTVVELYANIELPQDVDQVKENGATGVGLFRSEFLFLNRDSLPDEEEQF
EAYRTVARKMRGMPVTVRTFDLGADKNLDHAKRVAANPALGLRAIRLSLAEPQMFNIQLRAILRASRYGQIRILVPMLSN
VAEITQTLHLIQSAKQSLRNEKIPFDEKVQVGGMIEIPAAALSLDIFMRKLDFLSIGTNDLIQYTLAIDRADDTVAHLYD
SLHPAVLRLVAHVIRSANRASIPVSVCGEIAGDVVFTRLLLGFGLRVFSMHPVQLLTVKREVLRANLPDLIPITQKILKT
ADPEKIHALLAKLNA
>Mature_574_residues
SFVLHGVGVSEGIAIGHAHLASHAALEVAHHVVPEDQVTNEISRLDTAFTTVRKELQALHDTVVSGPAAAEYEAFLDLHR
MILDDPTLSTAAKAYIAQNQCNAEWAITQQMGVLMAQFEEIEDPYLRERKTDVIQVVERVLKVLLGHPGYIPPSQKRDGD
SVLVAHDLSPADVLQYKQYSFTAFLTDLGGLTSHTAIVARSLNIPSVVALHHARRLIRENDILIVDGNQGVVIVDPDEHV
LAEYRLRQSELELEKQKLKRLRTTVAATLDGTVVELYANIELPQDVDQVKENGATGVGLFRSEFLFLNRDSLPDEEEQFE
AYRTVARKMRGMPVTVRTFDLGADKNLDHAKRVAANPALGLRAIRLSLAEPQMFNIQLRAILRASRYGQIRILVPMLSNV
AEITQTLHLIQSAKQSLRNEKIPFDEKVQVGGMIEIPAAALSLDIFMRKLDFLSIGTNDLIQYTLAIDRADDTVAHLYDS
LHPAVLRLVAHVIRSANRASIPVSVCGEIAGDVVFTRLLLGFGLRVFSMHPVQLLTVKREVLRANLPDLIPITQKILKTA
DPEKIHALLAKLNA

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=571, Percent_Identity=37.4781085814361, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI48994992, Length=438, Percent_Identity=35.3881278538813, Blast_Score=275, Evalue=8e-75,
Organism=Escherichia coli, GI1789193, Length=496, Percent_Identity=33.8709677419355, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1788726, Length=571, Percent_Identity=28.1961471103327, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1787994, Length=396, Percent_Identity=25.2525252525253, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI226510935, Length=153, Percent_Identity=28.1045751633987, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63751; Mature: 63620

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFVLHGVGVSEGIAIGHAHLASHAALEVAHHVVPEDQVTNEISRLDTAFTTVRKELQAL
CCEEEECCCCCCCCEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HDTVVSGPAAAEYEAFLDLHRMILDDPTLSTAAKAYIAQNQCNAEWAITQQMGVLMAQFE
HHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHH
EIEDPYLRERKTDVIQVVERVLKVLLGHPGYIPPSQKRDGDSVLVAHDLSPADVLQYKQY
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHH
SFTAFLTDLGGLTSHTAIVARSLNIPSVVALHHARRLIRENDILIVDGNQGVVIVDPDEH
HHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCHH
VLAEYRLRQSELELEKQKLKRLRTTVAATLDGTVVELYANIELPQDVDQVKENGATGVGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCCEEH
FRSEFLFLNRDSLPDEEEQFEAYRTVARKMRGMPVTVRTFDLGADKNLDHAKRVAANPAL
HHHHEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHCCCCH
GLRAIRLSLAEPQMFNIQLRAILRASRYGQIRILVPMLSNVAEITQTLHLIQSAKQSLRN
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
EKIPFDEKVQVGGMIEIPAAALSLDIFMRKLDFLSIGTNDLIQYTLAIDRADDTVAHLYD
CCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEECCCHHHHHHHH
SLHPAVLRLVAHVIRSANRASIPVSVCGEIAGDVVFTRLLLGFGLRVFSMHPVQLLTVKR
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHH
EVLRANLPDLIPITQKILKTADPEKIHALLAKLNA
HHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SFVLHGVGVSEGIAIGHAHLASHAALEVAHHVVPEDQVTNEISRLDTAFTTVRKELQAL
CEEEECCCCCCCCEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HDTVVSGPAAAEYEAFLDLHRMILDDPTLSTAAKAYIAQNQCNAEWAITQQMGVLMAQFE
HHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHH
EIEDPYLRERKTDVIQVVERVLKVLLGHPGYIPPSQKRDGDSVLVAHDLSPADVLQYKQY
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHH
SFTAFLTDLGGLTSHTAIVARSLNIPSVVALHHARRLIRENDILIVDGNQGVVIVDPDEH
HHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCHH
VLAEYRLRQSELELEKQKLKRLRTTVAATLDGTVVELYANIELPQDVDQVKENGATGVGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCCEEH
FRSEFLFLNRDSLPDEEEQFEAYRTVARKMRGMPVTVRTFDLGADKNLDHAKRVAANPAL
HHHHEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHCCCCH
GLRAIRLSLAEPQMFNIQLRAILRASRYGQIRILVPMLSNVAEITQTLHLIQSAKQSLRN
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
EKIPFDEKVQVGGMIEIPAAALSLDIFMRKLDFLSIGTNDLIQYTLAIDRADDTVAHLYD
CCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEECCCHHHHHHHH
SLHPAVLRLVAHVIRSANRASIPVSVCGEIAGDVVFTRLLLGFGLRVFSMHPVQLLTVKR
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHH
EVLRANLPDLIPITQKILKTADPEKIHALLAKLNA
HHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653223 [H]