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The map label for this gene is btuF [H]

Identifier: 82701225

GI number: 82701225

Start: 92720

End: 93715

Strand: Reverse

Name: btuF [H]

Synonym: Nmul_A0090

Alternate gene names: 82701225

Gene position: 93715-92720 (Counterclockwise)

Preceding gene: 82701226

Following gene: 82701224

Centisome position: 2.94

GC content: 56.43

Gene sequence:

>996_bases
GTGATACGTCACGTCCTGATCCTGCTGTTCGCGTTGTTTACATCGGCTCTCATGGATTCCGGCAATGCTGGCGGCGTAAT
TCGACCGTCATCCTCCCCAGGTTTTGCCACTCCCGCAACAGGAACATGTTTATCGGGTTTTCCGACGACAGTGACGGACG
ATGACGGTCGCCGTGTTCGGATGACTCACGCTGCAATGCGCATCATTTCACTTTCACCCAACGTCACTGAACTGATATTT
GCTGCTGGAGCTGGGAAAAAACTGGTTGGAGTCAGCAGGCACAGCAATTATCCGGATGCCGCAAAATCCATTCCCGACAT
AGGGGACTCATTCAGTCTGGATCTTGAGAGAATTGTCGCACTACAGCCCGATCTGCTGCTGGCCTGGCGCAGTGGCAATG
CCAGAGCCGATATCGAGAAGCTGGAAAGACTTGGCCTGACTGTTTTTGCGACAGAAGCAGTCACGCTGGCCGATGTGTCG
CGCCTGCTGCGAATTATCGGCACGCTGGCGGGAACATCAGGGCCGGCGGAACGGGCAGCGAAAGCCTATGAAGAGGAATT
GCAGGGGATCCAGAGCAGCTACAGCAACCGGCCCAGGGTCAGCGTATTCCAGTTGATCTGGCATCAACCGCTGATGACGA
TAAACAACCAGCACCTCATCAGCGATGTCATCCAGATATGCGGTGGCACGAATGTGTTTGCGCGGGAATCTTCCCTGACG
CCGGTGGTGTCCGGGGAAAACCTGCTGGAGGCGGACCCGGATGCCATCATCAGCAGCGTCTCACTGGAGCAGGACGAAAG
CGCGCCCGCTGAAGTCAAGGCTTTCCTCCGACAGTTTTCGCCGCTCAGCGCGGTGAGGAATAACAATGTATTTTTTGTGC
ACCCCGATCTGATCCAACGGCAGACGACGCGTGTGTTGCGGGGCGCCCATCTGGTTTGCGAGCAGCTGGAATGCGTTCGT
TCCCGCCGGAAAATCGTCCGCCTGCCTGATTCCTGA

Upstream 100 bases:

>100_bases
AATAGACACCAGGCACGCCGCGCCGCCAGCTGCCTCAGCTTCCCCTTTCTCATTCTTTCCAGTCCTTCTCCATGGACGTG
CCGGAAGGGAGCAGCGTGAC

Downstream 100 bases:

>100_bases
TTTTCGTGCCCCCCCATGAGCCTGCACCGGCAGGCGCTCAGGAAAGCGGATTTGTCAATCAGGAGGATAAGAATTTCTCT
TGAGAAAACAGATGAATGGT

Product: periplasmic binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MIRHVLILLFALFTSALMDSGNAGGVIRPSSSPGFATPATGTCLSGFPTTVTDDDGRRVRMTHAAMRIISLSPNVTELIF
AAGAGKKLVGVSRHSNYPDAAKSIPDIGDSFSLDLERIVALQPDLLLAWRSGNARADIEKLERLGLTVFATEAVTLADVS
RLLRIIGTLAGTSGPAERAAKAYEEELQGIQSSYSNRPRVSVFQLIWHQPLMTINNQHLISDVIQICGGTNVFARESSLT
PVVSGENLLEADPDAIISSVSLEQDESAPAEVKAFLRQFSPLSAVRNNNVFFVHPDLIQRQTTRVLRGAHLVCEQLECVR
SRRKIVRLPDS

Sequences:

>Translated_331_residues
MIRHVLILLFALFTSALMDSGNAGGVIRPSSSPGFATPATGTCLSGFPTTVTDDDGRRVRMTHAAMRIISLSPNVTELIF
AAGAGKKLVGVSRHSNYPDAAKSIPDIGDSFSLDLERIVALQPDLLLAWRSGNARADIEKLERLGLTVFATEAVTLADVS
RLLRIIGTLAGTSGPAERAAKAYEEELQGIQSSYSNRPRVSVFQLIWHQPLMTINNQHLISDVIQICGGTNVFARESSLT
PVVSGENLLEADPDAIISSVSLEQDESAPAEVKAFLRQFSPLSAVRNNNVFFVHPDLIQRQTTRVLRGAHLVCEQLECVR
SRRKIVRLPDS
>Mature_331_residues
MIRHVLILLFALFTSALMDSGNAGGVIRPSSSPGFATPATGTCLSGFPTTVTDDDGRRVRMTHAAMRIISLSPNVTELIF
AAGAGKKLVGVSRHSNYPDAAKSIPDIGDSFSLDLERIVALQPDLLLAWRSGNARADIEKLERLGLTVFATEAVTLADVS
RLLRIIGTLAGTSGPAERAAKAYEEELQGIQSSYSNRPRVSVFQLIWHQPLMTINNQHLISDVIQICGGTNVFARESSLT
PVVSGENLLEADPDAIISSVSLEQDESAPAEVKAFLRQFSPLSAVRNNNVFFVHPDLIQRQTTRVLRGAHLVCEQLECVR
SRRKIVRLPDS

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=257, Percent_Identity=30.7392996108949, Blast_Score=132, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 35924; Mature: 35924

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRHVLILLFALFTSALMDSGNAGGVIRPSSSPGFATPATGTCLSGFPTTVTDDDGRRVR
CHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
MTHAAMRIISLSPNVTELIFAAGAGKKLVGVSRHSNYPDAAKSIPDIGDSFSLDLERIVA
HHHHHHHHHCCCCCHHHHHEECCCCCCEEEECCCCCCCHHHHCCCCCCCCCCHHHHHHHH
LQPDLLLAWRSGNARADIEKLERLGLTVFATEAVTLADVSRLLRIIGTLAGTSGPAERAA
CCCCEEEEECCCCCCHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KAYEEELQGIQSSYSNRPRVSVFQLIWHQPLMTINNQHLISDVIQICGGTNVFARESSLT
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHCCCHHHHHHHHHHHCCCCEEEECCCCC
PVVSGENLLEADPDAIISSVSLEQDESAPAEVKAFLRQFSPLSAVRNNNVFFVHPDLIQR
CEECCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHCCCCEEEECHHHHHH
QTTRVLRGAHLVCEQLECVRSRRKIVRLPDS
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
>Mature Secondary Structure
MIRHVLILLFALFTSALMDSGNAGGVIRPSSSPGFATPATGTCLSGFPTTVTDDDGRRVR
CHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
MTHAAMRIISLSPNVTELIFAAGAGKKLVGVSRHSNYPDAAKSIPDIGDSFSLDLERIVA
HHHHHHHHHCCCCCHHHHHEECCCCCCEEEECCCCCCCHHHHCCCCCCCCCCHHHHHHHH
LQPDLLLAWRSGNARADIEKLERLGLTVFATEAVTLADVSRLLRIIGTLAGTSGPAERAA
CCCCEEEEECCCCCCHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KAYEEELQGIQSSYSNRPRVSVFQLIWHQPLMTINNQHLISDVIQICGGTNVFARESSLT
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHCCCHHHHHHHHHHHCCCCEEEECCCCC
PVVSGENLLEADPDAIISSVSLEQDESAPAEVKAFLRQFSPLSAVRNNNVFFVHPDLIQR
CEECCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHCCCCEEEECHHHHHH
QTTRVLRGAHLVCEQLECVRSRRKIVRLPDS
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]