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The map label for this gene is ispH [H]

Identifier: 82701224

GI number: 82701224

Start: 91488

End: 92447

Strand: Reverse

Name: ispH [H]

Synonym: Nmul_A0089

Alternate gene names: 82701224

Gene position: 92447-91488 (Counterclockwise)

Preceding gene: 82701225

Following gene: 82701223

Centisome position: 2.9

GC content: 55.42

Gene sequence:

>960_bases
ATGGAAAACGGCGAAATGATCAAGGTATTGCTTGCAAACCCTCGCGGTTTCTGTGCAGGCGTGGACCGGGCAATCGAAAT
TGTCGAGCGTGCACTGAGCGTGCATGGCGCGCCGATCTATGTGCGCCACGAAGTGGTGCATAACCGTTTCGTAGTGGAGG
ATCTGGAAAAAAAGGGAGCAGTATTTGTCGAGAACCTCGATGAAGTTCCAGCCGGCAGCATTCTGATCTTCAGTGCTCAC
GGAGTTTCCCATGCGGTGCGACGCGAAGCCGAGGCGGGACAATTCAAGGTATTCGATGCCACCTGTCCGCTCGTGACCAA
AGTCCACATTGAAGTGGCAAAGATGCGGGACCAGGGAAGAGAAATCATCATGATCGGCCATGCGGGACATCCGGAGGTGG
AAGGTACCATGGGCCAGGCCGAGGGAGATGGTCCTTCCATGTATCTGGTGGAAACAGAAGAGGATGTCGAGAAACTGCAG
GTGCAGGATCCGGCTCACCTGGCTTACGTGACCCAGACCACCTTGTCCGTGGATGATGCCAATCGGGTGATCCAGGCCCT
GAAAGGCCGTTTCCCCAATATCCTCGGTCCCAAAAAGGATGATATCTGTTACGCCACCCAGAACCGGCAGGATGCGGTAA
AAACGATGGTCAAGCAGTGCGATCTCGTGATTGTGGTGGGCTCGCCCAATAGCTCCAACTCCAACCGTCTGTGTGAAGTC
GCGAGAAATGCCGGCGTGGACGCGTACATGCTGGACCGGGCGGAACAATTGCAGGAAGCCTGGCTGAAAGGCAAAATGCG
GATCGGTATTACCGCGGGGGCATCGGCGCCGGAAGTGCTTGTACAGGAAGTCATCTCGCGACTCAGACAAATCGTGTCAG
AGCAAGCGGAAAGGGAAGTGGTGGTGGAAGAGCTGAGAGGAGTGGTAGAGTCAGTGGTGTTCCCATTGCCAAGAAACTGA

Upstream 100 bases:

>100_bases
TCGCAGGCATGCAGAGGTTATACAAAGGATTAAATTGAGGGTATAGGTCGAATGCTGACCGTACCGCCAGCGGTATTTGT
GCTTGAATGAGGTGTATTTA

Downstream 100 bases:

>100_bases
TCACGGCTTTTCATCGGATAGTTTTACTTCCTTAGATCGGAAACACCTTGCTTCCGTCCATCAGTGCCCGCTCGGGCTGA
CGCGGCAACCTCACAACGAC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MENGEMIKVLLANPRGFCAGVDRAIEIVERALSVHGAPIYVRHEVVHNRFVVEDLEKKGAVFVENLDEVPAGSILIFSAH
GVSHAVRREAEAGQFKVFDATCPLVTKVHIEVAKMRDQGREIIMIGHAGHPEVEGTMGQAEGDGPSMYLVETEEDVEKLQ
VQDPAHLAYVTQTTLSVDDANRVIQALKGRFPNILGPKKDDICYATQNRQDAVKTMVKQCDLVIVVGSPNSSNSNRLCEV
ARNAGVDAYMLDRAEQLQEAWLKGKMRIGITAGASAPEVLVQEVISRLRQIVSEQAEREVVVEELRGVVESVVFPLPRN

Sequences:

>Translated_319_residues
MENGEMIKVLLANPRGFCAGVDRAIEIVERALSVHGAPIYVRHEVVHNRFVVEDLEKKGAVFVENLDEVPAGSILIFSAH
GVSHAVRREAEAGQFKVFDATCPLVTKVHIEVAKMRDQGREIIMIGHAGHPEVEGTMGQAEGDGPSMYLVETEEDVEKLQ
VQDPAHLAYVTQTTLSVDDANRVIQALKGRFPNILGPKKDDICYATQNRQDAVKTMVKQCDLVIVVGSPNSSNSNRLCEV
ARNAGVDAYMLDRAEQLQEAWLKGKMRIGITAGASAPEVLVQEVISRLRQIVSEQAEREVVVEELRGVVESVVFPLPRN
>Mature_319_residues
MENGEMIKVLLANPRGFCAGVDRAIEIVERALSVHGAPIYVRHEVVHNRFVVEDLEKKGAVFVENLDEVPAGSILIFSAH
GVSHAVRREAEAGQFKVFDATCPLVTKVHIEVAKMRDQGREIIMIGHAGHPEVEGTMGQAEGDGPSMYLVETEEDVEKLQ
VQDPAHLAYVTQTTLSVDDANRVIQALKGRFPNILGPKKDDICYATQNRQDAVKTMVKQCDLVIVVGSPNSSNSNRLCEV
ARNAGVDAYMLDRAEQLQEAWLKGKMRIGITAGASAPEVLVQEVISRLRQIVSEQAEREVVVEELRGVVESVVFPLPRN

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=312, Percent_Identity=58.974358974359, Blast_Score=382, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 35065; Mature: 35065

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENGEMIKVLLANPRGFCAGVDRAIEIVERALSVHGAPIYVRHEVVHNRFVVEDLEKKGA
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCC
VFVENLDEVPAGSILIFSAHGVSHAVRREAEAGQFKVFDATCPLVTKVHIEVAKMRDQGR
EEEECCCCCCCCCEEEEECCCHHHHHHHCCCCCCEEEEECCCCEEHHHHHHHHHHHHCCC
EIIMIGHAGHPEVEGTMGQAEGDGPSMYLVETEEDVEKLQVQDPAHLAYVTQTTLSVDDA
EEEEEECCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHCCCCCCEEEEEEEEEEHHHH
NRVIQALKGRFPNILGPKKDDICYATQNRQDAVKTMVKQCDLVIVVGSPNSSNSNRLCEV
HHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHH
ARNAGVDAYMLDRAEQLQEAWLKGKMRIGITAGASAPEVLVQEVISRLRQIVSEQAEREV
HHHCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
VVEELRGVVESVVFPLPRN
HHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MENGEMIKVLLANPRGFCAGVDRAIEIVERALSVHGAPIYVRHEVVHNRFVVEDLEKKGA
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCC
VFVENLDEVPAGSILIFSAHGVSHAVRREAEAGQFKVFDATCPLVTKVHIEVAKMRDQGR
EEEECCCCCCCCCEEEEECCCHHHHHHHCCCCCCEEEEECCCCEEHHHHHHHHHHHHCCC
EIIMIGHAGHPEVEGTMGQAEGDGPSMYLVETEEDVEKLQVQDPAHLAYVTQTTLSVDDA
EEEEEECCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHCCCCCCEEEEEEEEEEHHHH
NRVIQALKGRFPNILGPKKDDICYATQNRQDAVKTMVKQCDLVIVVGSPNSSNSNRLCEV
HHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHH
ARNAGVDAYMLDRAEQLQEAWLKGKMRIGITAGASAPEVLVQEVISRLRQIVSEQAEREV
HHHCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
VVEELRGVVESVVFPLPRN
HHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]