Click here to switch to the map view.

The map label for this gene is spoT [H]

Identifier: 82701185

GI number: 82701185

Start: 56539

End: 58686

Strand: Reverse

Name: spoT [H]

Synonym: Nmul_A0050

Alternate gene names: 82701185

Gene position: 58686-56539 (Counterclockwise)

Preceding gene: 82701186

Following gene: 82701184

Centisome position: 1.84

GC content: 56.38

Gene sequence:

>2148_bases
ATGCAGGCGCGAGGTCTGTCCAGTTCTTCTGCTACTTTGCCCGAGGCGAGTTTCCTTTTTCACGAACTCTCCAGCTATCT
CACGCGGGAAGACGTATCCGAGCTTCAGAACGCCTATCTCTTCAGTCAGAGCGCCCACTCGGGCCAATTCCGCCAATCCG
GCGAACCTTATATCTCTCATCCCCTGGCAGTAGCCAGCATCCTGGGCAAACTGCGCCTCGACAGCCAGACGCTGGCGGCA
GCGCTGCTGCACGACGTGATGGAAGATACGCACGTTTCCAAAGCTGAGATCGGGGATCGCTTTGGCAAGTCGGTCGCCGA
ACTGGTGGACGGTGTTTCCAAGCTGGACAAGATCGAATTCCAGAGCCACGCGGATGCGCAGGCGGAGAATTTCCGCAAAA
TGCTGCTGGCAATGGCGCGGGATGTGCGCGTCATTCTCATCAAGCTGGCGGATCGGTTGCACAATATGCGCACCCTGGAG
GCCATGCGGCCGGAGAAAAAGCGGCGCATCGCCCGTGAAACCATGGAGATCTACGCGCCTATTGCCAATCGGCTTGGGCT
CAACAATATTTACCATGAATTACAGGACTTAAGCTTCCGTTATCTGTATCCGAGGCGCTATCGGGTTCTGACCAACGCCA
CCAGGGCGGCGCGCGGAAACCGCCGCGAAATGGTGACGAAAATCCTCGAGACTATGAAGCAGCGCCTGAAGACCGCAGGG
TTGGATGCGGAAGTAACCGGCCGCGAGAAACAGCTCTACAGCATCTACAAGAAGATGGTGGAGAAGCATCTTTCGTTTTC
CGAGATTCTGGATATCTATGGTTTTCGCGTCATCGTAAAGGATGTGGCCTCGTGCTACATCGCGCTGGGCGCACTGCACA
GCCTCTACAATCCCATTCCCGGGAAATTCAAGGATTACATCGCCATCCCCAAGGCCAACGGCTACCAGTCCCTGCACAGC
ACCTTATGGGGGCCTTACGGCACGCCGATCGAAATCCAGATCCGGACACCGGAAATGCACCGGATTGCAGAGGCCGGCGT
TGCCTCGCACTGGCTCTACAAGAGCTCCGACGCGGATCTGAACGATTTGAACATGAAAACCCATCAGTGGTTGCAGAGCC
TGCTCGAAACCATGAGTGATTCCACCGATTCGCTGGAATTTCTGGAACACATCAAGGTTGACCTGTTTCCCGGCGAGGTC
TACGTCTTTACGCCCCAGGGCGAGATACTGGCACTGCCCAAGGGGTCGACTGCGGTGGATTTCGCCTATGCGGTGCATAC
GGACATCGGCGATTGTTGTGTTGCGGCAAAAATAAACGGGGAGAATGCGCCGCTGCGCACAGTGCTCAGAAGCGGAGACC
GTGTGGAAATTGTAACCGCCTCCCATGCCAAGCCTAATCCGGCGTGGCTGAATTACGTCGTTACCGGCAAGGCGCGCTCG
CATATCCGGCGTTTTCTCAAGACCATGCAATACGAGGAATCGGCGAAGCTCGGGGAGCGTCTGTTGAATCAGGCATTCGC
CACGCTCAAGGTCGATCCGCAGACAATCAGCGAGGCGCAGTGGGAAAAACTGACGCGTGAAAGCGGCGCCAAATCCAGAA
CCGATTTGCTGGCGGATATGGGCCTGGGTAAGCATTTACCGGCAGTGATTGCACGGCGGCTTGCGGCTTCACCCGGCGAA
TCCACTCTCGGGGCGAGCGGAGCTACGGATGTCATCACGATACTCGGTACCGAGGGCATGGCCGTACAATTTGCCAGGTG
CTGCCGGCCTATTCCCGGTGACCCGGTTATAGCGTTTATCAAGAAAGATCAGGGGCTCGTAATCCATACCGATGACTGTC
CGGCAGTAACCAAAAACAAGGGCAGTTCGGACAATTGGCTGGATGTGGCCTGGGGCAAGGACATCGACCGGCAGTTCGAA
GTGGGTATCAAGCTGATGGTGGCCAACCAGCGAGGAGTACTCGCCAGGGTGGCGGCAGCCATCGCTGATGCGGATTCCAA
TATCGATAATGTCGCCATGGAGGGCGATGGCGCATATACCAATATGAATTTCGTCTTGCAGGTACGCAATCGTCATCATC
TGGCACAAGTGATTCGCAGTTTGAGGCGGATTCCTGAAGTGGCGAAGATCAGCCGCGTGAAGGAGTGA

Upstream 100 bases:

>100_bases
GTTTCATCGCGTAACAAGTAGACTGACGATCAATCATCAAGTGAGGATACCGCTGCCATTCCGGAACGTATGTTAATGGA
ATGAAATGAAGGAAATGACG

Downstream 100 bases:

>100_bases
CAGGAACGGCGAGCAAGCTTGTTTCTCCCCCGGACACCCATAACGGGGCGAGGATTGAAGCCCCTATGAAGGCGAGGCAG
GGGAAGTCATGAACCCAAGG

Product: (p)ppGpp synthetase SpoT/RelA

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 715; Mature: 715

Protein sequence:

>715_residues
MQARGLSSSSATLPEASFLFHELSSYLTREDVSELQNAYLFSQSAHSGQFRQSGEPYISHPLAVASILGKLRLDSQTLAA
ALLHDVMEDTHVSKAEIGDRFGKSVAELVDGVSKLDKIEFQSHADAQAENFRKMLLAMARDVRVILIKLADRLHNMRTLE
AMRPEKKRRIARETMEIYAPIANRLGLNNIYHELQDLSFRYLYPRRYRVLTNATRAARGNRREMVTKILETMKQRLKTAG
LDAEVTGREKQLYSIYKKMVEKHLSFSEILDIYGFRVIVKDVASCYIALGALHSLYNPIPGKFKDYIAIPKANGYQSLHS
TLWGPYGTPIEIQIRTPEMHRIAEAGVASHWLYKSSDADLNDLNMKTHQWLQSLLETMSDSTDSLEFLEHIKVDLFPGEV
YVFTPQGEILALPKGSTAVDFAYAVHTDIGDCCVAAKINGENAPLRTVLRSGDRVEIVTASHAKPNPAWLNYVVTGKARS
HIRRFLKTMQYEESAKLGERLLNQAFATLKVDPQTISEAQWEKLTRESGAKSRTDLLADMGLGKHLPAVIARRLAASPGE
STLGASGATDVITILGTEGMAVQFARCCRPIPGDPVIAFIKKDQGLVIHTDDCPAVTKNKGSSDNWLDVAWGKDIDRQFE
VGIKLMVANQRGVLARVAAAIADADSNIDNVAMEGDGAYTNMNFVLQVRNRHHLAQVIRSLRRIPEVAKISRVKE

Sequences:

>Translated_715_residues
MQARGLSSSSATLPEASFLFHELSSYLTREDVSELQNAYLFSQSAHSGQFRQSGEPYISHPLAVASILGKLRLDSQTLAA
ALLHDVMEDTHVSKAEIGDRFGKSVAELVDGVSKLDKIEFQSHADAQAENFRKMLLAMARDVRVILIKLADRLHNMRTLE
AMRPEKKRRIARETMEIYAPIANRLGLNNIYHELQDLSFRYLYPRRYRVLTNATRAARGNRREMVTKILETMKQRLKTAG
LDAEVTGREKQLYSIYKKMVEKHLSFSEILDIYGFRVIVKDVASCYIALGALHSLYNPIPGKFKDYIAIPKANGYQSLHS
TLWGPYGTPIEIQIRTPEMHRIAEAGVASHWLYKSSDADLNDLNMKTHQWLQSLLETMSDSTDSLEFLEHIKVDLFPGEV
YVFTPQGEILALPKGSTAVDFAYAVHTDIGDCCVAAKINGENAPLRTVLRSGDRVEIVTASHAKPNPAWLNYVVTGKARS
HIRRFLKTMQYEESAKLGERLLNQAFATLKVDPQTISEAQWEKLTRESGAKSRTDLLADMGLGKHLPAVIARRLAASPGE
STLGASGATDVITILGTEGMAVQFARCCRPIPGDPVIAFIKKDQGLVIHTDDCPAVTKNKGSSDNWLDVAWGKDIDRQFE
VGIKLMVANQRGVLARVAAAIADADSNIDNVAMEGDGAYTNMNFVLQVRNRHHLAQVIRSLRRIPEVAKISRVKE
>Mature_715_residues
MQARGLSSSSATLPEASFLFHELSSYLTREDVSELQNAYLFSQSAHSGQFRQSGEPYISHPLAVASILGKLRLDSQTLAA
ALLHDVMEDTHVSKAEIGDRFGKSVAELVDGVSKLDKIEFQSHADAQAENFRKMLLAMARDVRVILIKLADRLHNMRTLE
AMRPEKKRRIARETMEIYAPIANRLGLNNIYHELQDLSFRYLYPRRYRVLTNATRAARGNRREMVTKILETMKQRLKTAG
LDAEVTGREKQLYSIYKKMVEKHLSFSEILDIYGFRVIVKDVASCYIALGALHSLYNPIPGKFKDYIAIPKANGYQSLHS
TLWGPYGTPIEIQIRTPEMHRIAEAGVASHWLYKSSDADLNDLNMKTHQWLQSLLETMSDSTDSLEFLEHIKVDLFPGEV
YVFTPQGEILALPKGSTAVDFAYAVHTDIGDCCVAAKINGENAPLRTVLRSGDRVEIVTASHAKPNPAWLNYVVTGKARS
HIRRFLKTMQYEESAKLGERLLNQAFATLKVDPQTISEAQWEKLTRESGAKSRTDLLADMGLGKHLPAVIARRLAASPGE
STLGASGATDVITILGTEGMAVQFARCCRPIPGDPVIAFIKKDQGLVIHTDDCPAVTKNKGSSDNWLDVAWGKDIDRQFE
VGIKLMVANQRGVLARVAAAIADADSNIDNVAMEGDGAYTNMNFVLQVRNRHHLAQVIRSLRRIPEVAKISRVKE

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance [H]

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=704, Percent_Identity=49.2897727272727, Blast_Score=707, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=686, Percent_Identity=31.6326530612245, Blast_Score=385, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25143118, Length=134, Percent_Identity=35.8208955223881, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 79696; Mature: 79696

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQARGLSSSSATLPEASFLFHELSSYLTREDVSELQNAYLFSQSAHSGQFRQSGEPYISH
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
PLAVASILGKLRLDSQTLAAALLHDVMEDTHVSKAEIGDRFGKSVAELVDGVSKLDKIEF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSHADAQAENFRKMLLAMARDVRVILIKLADRLHNMRTLEAMRPEKKRRIARETMEIYAP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IANRLGLNNIYHELQDLSFRYLYPRRYRVLTNATRAARGNRREMVTKILETMKQRLKTAG
HHHHCCHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
LDAEVTGREKQLYSIYKKMVEKHLSFSEILDIYGFRVIVKDVASCYIALGALHSLYNPIP
CCCEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GKFKDYIAIPKANGYQSLHSTLWGPYGTPIEIQIRTPEMHRIAEAGVASHWLYKSSDADL
CCCCCEEECCCCCCHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHEECCCCCCC
NDLNMKTHQWLQSLLETMSDSTDSLEFLEHIKVDLFPGEVYVFTPQGEILALPKGSTAVD
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCEEEEECCEEEEECCCCCEEEECCCCCCEE
FAYAVHTDIGDCCVAAKINGENAPLRTVLRSGDRVEIVTASHAKPNPAWLNYVVTGKARS
EHHEECCCCCCEEEEEEECCCCCHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCHHH
HIRRFLKTMQYEESAKLGERLLNQAFATLKVDPQTISEAQWEKLTRESGAKSRTDLLADM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHC
GLGKHLPAVIARRLAASPGESTLGASGATDVITILGTEGMAVQFARCCRPIPGDPVIAFI
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCEEEEE
KKDQGLVIHTDDCPAVTKNKGSSDNWLDVAWGKDIDRQFEVGIKLMVANQRGVLARVAAA
ECCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCEEEEEECCCCHHHHHHHH
IADADSNIDNVAMEGDGAYTNMNFVLQVRNRHHLAQVIRSLRRIPEVAKISRVKE
HHHCCCCCCCEEECCCCCEECCEEEEEEHHHHHHHHHHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure
MQARGLSSSSATLPEASFLFHELSSYLTREDVSELQNAYLFSQSAHSGQFRQSGEPYISH
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
PLAVASILGKLRLDSQTLAAALLHDVMEDTHVSKAEIGDRFGKSVAELVDGVSKLDKIEF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSHADAQAENFRKMLLAMARDVRVILIKLADRLHNMRTLEAMRPEKKRRIARETMEIYAP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IANRLGLNNIYHELQDLSFRYLYPRRYRVLTNATRAARGNRREMVTKILETMKQRLKTAG
HHHHCCHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
LDAEVTGREKQLYSIYKKMVEKHLSFSEILDIYGFRVIVKDVASCYIALGALHSLYNPIP
CCCEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GKFKDYIAIPKANGYQSLHSTLWGPYGTPIEIQIRTPEMHRIAEAGVASHWLYKSSDADL
CCCCCEEECCCCCCHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHEECCCCCCC
NDLNMKTHQWLQSLLETMSDSTDSLEFLEHIKVDLFPGEVYVFTPQGEILALPKGSTAVD
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCEEEEECCEEEEECCCCCEEEECCCCCCEE
FAYAVHTDIGDCCVAAKINGENAPLRTVLRSGDRVEIVTASHAKPNPAWLNYVVTGKARS
EHHEECCCCCCEEEEEEECCCCCHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCHHH
HIRRFLKTMQYEESAKLGERLLNQAFATLKVDPQTISEAQWEKLTRESGAKSRTDLLADM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHC
GLGKHLPAVIARRLAASPGESTLGASGATDVITILGTEGMAVQFARCCRPIPGDPVIAFI
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCEEEEE
KKDQGLVIHTDDCPAVTKNKGSSDNWLDVAWGKDIDRQFEVGIKLMVANQRGVLARVAAA
ECCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCEEEEEECCCCHHHHHHHH
IADADSNIDNVAMEGDGAYTNMNFVLQVRNRHHLAQVIRSLRRIPEVAKISRVKE
HHHCCCCCCCEEECCCCCEECCEEEEEEHHHHHHHHHHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]