Click here to switch to the map view.

The map label for this gene is intZ [H]

Identifier: 82546008

GI number: 82546008

Start: 3663101

End: 3664588

Strand: Direct

Name: intZ [H]

Synonym: SBO_3647

Alternate gene names: 82546008

Gene position: 3663101-3664588 (Clockwise)

Preceding gene: 82546007

Following gene: 82546009

Centisome position: 81.05

GC content: 50.94

Gene sequence:

>1488_bases
TTGAGCAGATGCGCGAGCAGATTCAGAATATCGAGTAAGCTATCAGCAACATTTAGGACAACGCATAAATCACACCAAGC
CCGCAACCCAGCGGGCTTTTTTGTTATCATGGGCTATCATAAAGACGCAACAAAGCGCATGATTATGGTGTACACAAAGG
TGTACCCAATGAGGTTTGGGTACATTGCTTTGGGTACACCCCCTATTCAAAGTTTGGATATTCGCAAAATGCCAAAACTA
ACAGACATGCAGATCCGCGCATGGATTAAGAGCGGAGAGCGATTCGAGGGGCGGGCAGACGGTAACGGTTTATACCTACG
TTACCGTGAAGCCGACAAAACCCCCACATGGAGATTCCGCTATAAACTCGCAGGGAAGTCCCGCGCCATGCTAATTGGTT
CGTATAGCGAGCTATCACTATCAAAGGCCAGAGAGACAGCCAAAGAGCTATCGGCTCGCGTTGCGCTGGGCTATGACGTT
GCAGGAGAGAAGCAGAAGCGCAAAACCGAAGCACTGGCGAAGATGGAAGCAGAGAAGAACGCTATGCGCGTTTCAGAGCT
TGCCGCTGAATACTTTGAGCGTCAGATCCTCCCGCGCTGGAAGCACCCCGATATACTCCGCCGCCGTATCGACAAAGATA
TAAACCCCTGCATTGGCAGCATGAAGGTAGAGGACGTGAAACCGCGCCATATCGATGACATGCTGAAAGGTATTGTTGAC
CGTGGAGCGCCGACCATAGCAACGGACGTGCTGAGATGGACGCGCCGCATATTCGACTACGGAATCAAACGGCACGCGCT
AGAGATTAACCCCTGTTCAGCCTTTGAGGTGGCAGACGCCGGAGGGAAAGAAGCTGCCCGTGACCGCTGGTTAACCCGCG
ATGAGTTAATCCAGCTATTCAAAGCCATGCGCACGGCTAAGGGATTCAGTCGCCAGAACGAAATCACGTTCAAATTACTG
TTAGCGTTATGCGTCCGCAAAATGGAATTATGCGCCGCACGATGGGAAGAGTTTGATTTAGATGGTGCGGTATGGCATTT
GCCGGAAGAACGCAGCAAAAACGGAGACCCTATTGATATACCTCTACCTTCCCCAGCCGTTGAATGGTTGAGAGAGCTAC
ACACCTTTTCATGTAATAGCGCATGGGTGCTTCCGGCCAGGAAGATGCAAAACAGAATGATCCCACATATTCAGGAAAGC
ACTTTACCCGTAGCACTGGCTAAGGTTCGCGCCGAAATGCCGGATGTGCCTAATTTCACGATTCACGACTTTCGACGCAC
CGCACGTACTCATTTAGCAGCGTTGGGTGTTGATCCTGTTGTGGCGGAACGATGCCTCAATCATCGCATTAAGGGCGTAG
AGGGGATTTATAACCGCCATCAGTATTTTGATGAGCGTAAAGCAGCACTGGCACAGTGGGCTGATCTGCTGGTGGCACTG
GAAAGCGGAAAAGACTACAACGTAACGCCTCTCAGAAGGGCGAACTAA

Upstream 100 bases:

>100_bases
GACTTTATGATGCAGGAATTCAACCGCGAGTCGAACACTCTTGCGTCGAAGTCTATCAATGCCGAAGTGACAAACTCCGC
GATTGAGCTGAAAGTGTTGA

Downstream 100 bases:

>100_bases
CTGAATAGATTGAGCCTAGCTCGACGGGGCGAAAAGCGGGAAGCCTTACCGCCTGGCTCAATCACAAAATAAGGCGCAGC
GTAAAAGGTGACACAATGAA

Product: integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSRCASRFRISSKLSATFRTTHKSHQARNPAGFFVIMGYHKDATKRMIMVYTKVYPMRFGYIALGTPPIQSLDIRKMPKL
TDMQIRAWIKSGERFEGRADGNGLYLRYREADKTPTWRFRYKLAGKSRAMLIGSYSELSLSKARETAKELSARVALGYDV
AGEKQKRKTEALAKMEAEKNAMRVSELAAEYFERQILPRWKHPDILRRRIDKDINPCIGSMKVEDVKPRHIDDMLKGIVD
RGAPTIATDVLRWTRRIFDYGIKRHALEINPCSAFEVADAGGKEAARDRWLTRDELIQLFKAMRTAKGFSRQNEITFKLL
LALCVRKMELCAARWEEFDLDGAVWHLPEERSKNGDPIDIPLPSPAVEWLRELHTFSCNSAWVLPARKMQNRMIPHIQES
TLPVALAKVRAEMPDVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFDERKAALAQWADLLVAL
ESGKDYNVTPLRRAN

Sequences:

>Translated_495_residues
MSRCASRFRISSKLSATFRTTHKSHQARNPAGFFVIMGYHKDATKRMIMVYTKVYPMRFGYIALGTPPIQSLDIRKMPKL
TDMQIRAWIKSGERFEGRADGNGLYLRYREADKTPTWRFRYKLAGKSRAMLIGSYSELSLSKARETAKELSARVALGYDV
AGEKQKRKTEALAKMEAEKNAMRVSELAAEYFERQILPRWKHPDILRRRIDKDINPCIGSMKVEDVKPRHIDDMLKGIVD
RGAPTIATDVLRWTRRIFDYGIKRHALEINPCSAFEVADAGGKEAARDRWLTRDELIQLFKAMRTAKGFSRQNEITFKLL
LALCVRKMELCAARWEEFDLDGAVWHLPEERSKNGDPIDIPLPSPAVEWLRELHTFSCNSAWVLPARKMQNRMIPHIQES
TLPVALAKVRAEMPDVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFDERKAALAQWADLLVAL
ESGKDYNVTPLRRAN
>Mature_494_residues
SRCASRFRISSKLSATFRTTHKSHQARNPAGFFVIMGYHKDATKRMIMVYTKVYPMRFGYIALGTPPIQSLDIRKMPKLT
DMQIRAWIKSGERFEGRADGNGLYLRYREADKTPTWRFRYKLAGKSRAMLIGSYSELSLSKARETAKELSARVALGYDVA
GEKQKRKTEALAKMEAEKNAMRVSELAAEYFERQILPRWKHPDILRRRIDKDINPCIGSMKVEDVKPRHIDDMLKGIVDR
GAPTIATDVLRWTRRIFDYGIKRHALEINPCSAFEVADAGGKEAARDRWLTRDELIQLFKAMRTAKGFSRQNEITFKLLL
ALCVRKMELCAARWEEFDLDGAVWHLPEERSKNGDPIDIPLPSPAVEWLRELHTFSCNSAWVLPARKMQNRMIPHIQEST
LPVALAKVRAEMPDVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFDERKAALAQWADLLVALE
SGKDYNVTPLRRAN

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI145693166, Length=391, Percent_Identity=27.3657289002558, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788974, Length=418, Percent_Identity=27.2727272727273, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1788690, Length=405, Percent_Identity=27.1604938271605, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 56914; Mature: 56782

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRCASRFRISSKLSATFRTTHKSHQARNPAGFFVIMGYHKDATKRMIMVYTKVYPMRFG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHEEC
YIALGTPPIQSLDIRKMPKLTDMQIRAWIKSGERFEGRADGNGLYLRYREADKTPTWRFR
EEEECCCCCCCCCHHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCEEEE
YKLAGKSRAMLIGSYSELSLSKARETAKELSARVALGYDVAGEKQKRKTEALAKMEAEKN
EEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
AMRVSELAAEYFERQILPRWKHPDILRRRIDKDINPCIGSMKVEDVKPRHIDDMLKGIVD
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHCCCCCCCCCCCHHHHHHHHHHH
RGAPTIATDVLRWTRRIFDYGIKRHALEINPCSAFEVADAGGKEAARDRWLTRDELIQLF
CCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCCCEEECCCCCHHHHHHHCCCHHHHHHHH
KAMRTAKGFSRQNEITFKLLLALCVRKMELCAARWEEFDLDGAVWHLPEERSKNGDPIDI
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHCCCCCEEEC
PLPSPAVEWLRELHTFSCNSAWVLPARKMQNRMIPHIQESTLPVALAKVRAEMPDVPNFT
CCCCHHHHHHHHHHHCCCCCEEECCHHHHHCCCCCCCHHCCHHHHHHHHHHHCCCCCCCH
IHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFDERKAALAQWADLLVAL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESGKDYNVTPLRRAN
HCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRCASRFRISSKLSATFRTTHKSHQARNPAGFFVIMGYHKDATKRMIMVYTKVYPMRFG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHEEC
YIALGTPPIQSLDIRKMPKLTDMQIRAWIKSGERFEGRADGNGLYLRYREADKTPTWRFR
EEEECCCCCCCCCHHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCEEEE
YKLAGKSRAMLIGSYSELSLSKARETAKELSARVALGYDVAGEKQKRKTEALAKMEAEKN
EEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
AMRVSELAAEYFERQILPRWKHPDILRRRIDKDINPCIGSMKVEDVKPRHIDDMLKGIVD
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHCCCCCCCCCCCHHHHHHHHHHH
RGAPTIATDVLRWTRRIFDYGIKRHALEINPCSAFEVADAGGKEAARDRWLTRDELIQLF
CCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCCCEEECCCCCHHHHHHHCCCHHHHHHHH
KAMRTAKGFSRQNEITFKLLLALCVRKMELCAARWEEFDLDGAVWHLPEERSKNGDPIDI
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHCCCCCEEEC
PLPSPAVEWLRELHTFSCNSAWVLPARKMQNRMIPHIQESTLPVALAKVRAEMPDVPNFT
CCCCHHHHHHHHHHHCCCCCEEECCHHHHHCCCCCCCHHCCHHHHHHHHHHHCCCCCCCH
IHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFDERKAALAQWADLLVAL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESGKDYNVTPLRRAN
HCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]