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The map label for this gene is insB [H]

Identifier: 82545621

GI number: 82545621

Start: 3250208

End: 3250603

Strand: Reverse

Name: insB [H]

Synonym: SBO_3244

Alternate gene names: 82545621

Gene position: 3250603-3250208 (Counterclockwise)

Preceding gene: 82545624

Following gene: 82545617

Centisome position: 71.92

GC content: 52.78

Gene sequence:

>396_bases
GTGATTGTCTGCGCTGAAATGGACGAACAGTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTA
TGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGC
TGTCGGCCTTTGAGGTCGTGGTATGGATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTT
ATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGCTGGGACGGAAGTC
ACTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAA

Upstream 100 bases:

>100_bases
TCTGTACTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATACTGGTTGTCTGGAGATTCAG
GGGGCCAGTCTAGCAGTGAT

Downstream 100 bases:

>100_bases
GTTGGAGTCATTACCGAAATGGGCTGCAATCAATGATTATGGCGGCAGTTCCGGGACAGAAACTCGTCCACTGCAATAAC
AAATATAAAAAACACAGGTC

Product: IS1 ORF2

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 131; Mature: 131

Protein sequence:

>131_residues
MIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHV
ISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ

Sequences:

>Translated_131_residues
MIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHV
ISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
>Mature_131_residues
MIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHV
ISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=131, Percent_Identity=92.3664122137405, Blast_Score=256, Evalue=2e-70,
Organism=Escherichia coli, GI1788203, Length=131, Percent_Identity=92.3664122137405, Blast_Score=256, Evalue=2e-70,
Organism=Escherichia coli, GI1786203, Length=131, Percent_Identity=92.3664122137405, Blast_Score=256, Evalue=2e-70,
Organism=Escherichia coli, GI1787223, Length=131, Percent_Identity=92.3664122137405, Blast_Score=256, Evalue=3e-70,
Organism=Escherichia coli, GI1786470, Length=131, Percent_Identity=92.3664122137405, Blast_Score=256, Evalue=3e-70,
Organism=Escherichia coli, GI1786459, Length=131, Percent_Identity=92.3664122137405, Blast_Score=256, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 15615; Mature: 15615

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVV
CEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEE
VWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKV
EEECCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHCCHHHHHHH
IGHYLNIKHYQ
HHHHHCCCCCC
>Mature Secondary Structure
MIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVV
CEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEE
VWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKV
EEECCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHCCHHHHHHH
IGHYLNIKHYQ
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]