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The map label for this gene is rpoS [H]

Identifier: 82545186

GI number: 82545186

Start: 2785680

End: 2786675

Strand: Direct

Name: rpoS [H]

Synonym: SBO_2779

Alternate gene names: 82545186

Gene position: 2785680-2786675 (Clockwise)

Preceding gene: 82545185

Following gene: 82545192

Centisome position: 61.63

GC content: 51.91

Gene sequence:

>996_bases
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGAG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGAAGTTTATTTT
GCGCGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGC
CCGCCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGA
AGTTTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATG
AACCAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTC
CCATAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGC
TTCGTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCC
GATGAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCT
GAACGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTC
GTGAAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGTGAAATCCTGCAAACG
CAGGGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Upstream 100 bases:

>100_bases
AATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGTTCCGTCAAGGGA
TCACGGGTAGGAGCCACCTT

Downstream 100 bases:

>100_bases
GTAAGCATCTGTCAGAAAGGCCAGTCTCAAGCGAGGCTGGCCTTTTCTGTGCACAATAAAAGGTCCGATGCCCATCGGAC
CTTTTTATTAAGGTCAAATT

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEEVYF
ARRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIM
NQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILA
DEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQT
QGLNIEALFRE

Sequences:

>Translated_331_residues
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEEVYF
ARRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIM
NQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILA
DEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQT
QGLNIEALFRE
>Mature_330_residues
SQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for the normal expression of the enterotoxin, yst, in the stationary phase [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=331, Percent_Identity=99.3957703927493, Blast_Score=653, Evalue=0.0,
Organism=Escherichia coli, GI1789448, Length=237, Percent_Identity=42.6160337552743, Blast_Score=207, Evalue=6e-55,
Organism=Escherichia coli, GI1789871, Length=268, Percent_Identity=26.4925373134328, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 38103; Mature: 37971

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQL
CCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHH
YLGEIGYSPLLTAEEEEVYFARRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDL
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHH
IEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYL
HHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
RTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILA
HHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
DEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTR
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCH
ERVRQIQVEGLRRLREILQTQGLNIEALFRE
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
>Mature Secondary Structure 
SQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQL
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHH
YLGEIGYSPLLTAEEEEVYFARRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDL
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHH
IEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYL
HHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
RTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILA
HHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
DEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTR
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCH
ERVRQIQVEGLRRLREILQTQGLNIEALFRE
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7729893 [H]