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The map label for this gene is ypfI [H]

Identifier: 82544922

GI number: 82544922

Start: 2483888

End: 2485903

Strand: Reverse

Name: ypfI [H]

Synonym: SBO_2491

Alternate gene names: 82544922

Gene position: 2485903-2483888 (Counterclockwise)

Preceding gene: 82544923

Following gene: 161984890

Centisome position: 55.0

GC content: 54.96

Gene sequence:

>2016_bases
ATGGCTGAACTGACTGCGCTTTACACATTAACAGCGCAAATGAAACGTGAAGGGATCCGCCGCTTGCTGGTGTTGAGCGG
GGAAGAGGGTTGGTGTTTTGATCATGCGCTTAAGTTGCGTGATGCCTTACTTGGCGACTGGCTGTGGATTTCGCCGCAGC
CAGATGCTGAAAACCACTGTTCTCCCTCGGCGCTACAAACTTTACTTGGGCGCGAGTTCCGGCATGCGGTATTCGACGCC
CGCCACGGCTTTGATGCCGCTGCCTTTGCGGCACTTAGCGGAACGTTGAAAGCGGGAAGCTGGCTGGTTTTGTTACTCCC
TGTATGGGAAGAGTGGGAAAACCAACCTGATGCCGACTCGCTGCGCTGGAGTGATTGCCCTGACCCTATTGCGACGCCGC
ATTTTGTCCAGCATCTCAAACGCGTACTTACGGCGGATAACGACGCTATTCTCTGGCGGCAAAACCAGCCGTTCTCGTTG
GCGCATTTTACTCCCCGTACTGACTGGCACCCCGCGACCGGCGCACCACAACCAGAACAACAGCAACTCTTACAGCAGCT
ACTGACCATGCCGCCGGGCGTGGCAGCGGTAACGGCTGCGCGTGGGCGCGGTAAGTCGGCGCTGGCAGGGCAACTCATTT
CTCGTATTGCGGGCAGTGCGATTGTCACCGCGCCCGCAAAAGCGGCAACGGATGTACTGGCACAATTTGCGGGCGAGAAG
TTTCGCTTTATTGCGCCGGATGCCTTGTTAGCCAGCGATGAGCAAGCCGACTGGCTGGTGGTCGATGAAGCCGCAGCCAT
ACCTGCGCCGTTGTTGCATCAACTGGTATCGCGTTTTCCTCGAACGTTGTTAACCACTACGGTGCAGGGCTACGAAGGCA
CCGGACGTGGTTTTTTGCTGAAATTTTGCGCTCGCTTTCCGCATTTACACCGTTTTGAACTGCAACAGCCGATCCGCTGG
GCACAGGGATGCCCGCTGGAAAAAATGGTTAGTGAGGCACTGGTTTTTGACGATGAAAACTTCACCCATACACCACAAGG
CAATATCGTCATTTCCGCATTTGAACAAACGTTATGGCGAAGCGAGCCAGAAACGCCGTTAAAGGTTTATCAGTTATTGT
CTGGTGCGCACTACCGGACTTCGCCGCTGGATTTACGCCGGATGATGGATGCACCAGGGCAATATTTTTTACAGGCGGCT
GGCGAAAACGAGATTGCCGGAGCGCTGTGGCTGGTGGATGAGGGGGGATTATCTCAACAACTCAGTCAGGCGGTATGGGC
AGGTTTTCGTCGCCCGCGGGGTAATCTGGTGGCCCAGTCGCTGGCGGCGCACGGTAGCAATCCACTGGCAGCGACATTGC
GTGGACGGCGGGTCAGCCGGATAGCAGTTCATCCGGCTCGTCAGCGCGAAGGCGTTGGGCAACAGCTCATTGCCAGCGCT
TTGCAATATACGCAAGACCTCGACTATCTTTCGGTGAGTTTTGGTTACACCGGGGAGTTATGGCGTTTCTGGCAACGCTG
CGGTTTTGTGCTGGTACGAATGGGTAATCATCGTGAAGCCAGCAGTGGTTGCTATACGGCGATGGCGCTGTTACCGATGA
GTGATGCGGGTAAACAGCTGGCTGAACGTGAGCATTACTGTTTACGTCGCGATGCGCAAGCTCTCGCGCAGTGGAATGGC
GAAACACTTCCTGTTGATCCACTAAACGATATCGTGCTTTCTGACGACGACTGGCTTGAACTGGCCGGTTTTGCTTTTGC
TCATCGTCCGCTATTAACGTCGTTAGGTTGCTTATTGCGTCTGCTACAAACCAGTGAACTGGCATTACCGGCGCTGCGTG
GGCGTTTACAGAAAAATGCCAGTGATGCGCAGTTATGTACCACACTTAAACTTGCAGGCCGCAAGATGTTACTGGTCCGT
CAGCGGGAAGAGGCCGCGCATGCGCTGTTCGCACTTAATGATGTTCGCACTGAGCGTCTGCGCGATCGCATAACGCAATG
GCAATTTTTTCACTGA

Upstream 100 bases:

>100_bases
CTTCTCAGCAACGCTACAGCTGGTTTAAACGTGGTTTCGACAGCGGCGATCCGGCACAATGCAATACTTTTGGTAAAAGC
ATTTAATTTCCGGGGCAGGG

Downstream 100 bases:

>100_bases
CTCCTTCAATTTTCTCCCATGGTCATTGTGAGCCAGCGGCGTAAAATTAATGACATCAGTTAAGGAGTTCGCCATGAAAC
ATGACCATTTTGTTGTTCAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 670

Protein sequence:

>671_residues
MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALLGDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDA
RHGFDAAAFAALSGTLKAGSWLVLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATDVLAQFAGEK
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRW
AQGCPLEKMVSEALVFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQYFLQAA
GENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGVGQQLIASA
LQYTQDLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYCLRRDAQALAQWNG
ETLPVDPLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQLCTTLKLAGRKMLLVR
QREEAAHALFALNDVRTERLRDRITQWQFFH

Sequences:

>Translated_671_residues
MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALLGDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDA
RHGFDAAAFAALSGTLKAGSWLVLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATDVLAQFAGEK
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRW
AQGCPLEKMVSEALVFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQYFLQAA
GENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGVGQQLIASA
LQYTQDLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYCLRRDAQALAQWNG
ETLPVDPLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQLCTTLKLAGRKMLLVR
QREEAAHALFALNDVRTERLRDRITQWQFFH
>Mature_670_residues
AELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALLGDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDAR
HGFDAAAFAALSGTLKAGSWLVLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSLA
HFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATDVLAQFAGEKF
RFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWA
QGCPLEKMVSEALVFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQYFLQAAG
ENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGVGQQLIASAL
QYTQDLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYCLRRDAQALAQWNGE
TLPVDPLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQLCTTLKLAGRKMLLVRQ
REEAAHALFALNDVRTERLRDRITQWQFFH

Specific function: Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP). It recognizes the wobble base of tRNA(Met), thus distinguishing between tRNA(Met) and the structurally si

COG id: COG1444

COG function: function code R; Predicted P-loop ATPase fused to an acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1788818, Length=671, Percent_Identity=96.5722801788376, Blast_Score=1319, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17507621, Length=367, Percent_Identity=25.6130790190736, Blast_Score=87, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324197, Length=379, Percent_Identity=25.8575197889182, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24640662, Length=351, Percent_Identity=26.4957264957265, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR013562
- InterPro:   IPR007807 [H]

Pfam domain/function: PF08351 DUF1726; PF05127 DUF699 [H]

EC number: NA

Molecular weight: Translated: 74777; Mature: 74646

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALLGDWLWISPQPDAENHC
CCCHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCC
SPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWLVLLLPVWEEWENQPDADS
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEEECHHHHCCCCCCCC
LRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSLAHFTPRTDWHPATGAPQPEQ
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCHHH
QQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATDVLAQFAGEK
HHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCCC
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLL
EEEECCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
KFCARFPHLHRFELQQPIRWAQGCPLEKMVSEALVFDDENFTHTPQGNIVISAFEQTLWR
HHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCCCCCCCEEEEHHHHHHHC
SEPETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQYFLQAAGENEIAGALWLVDEGGLSQQ
CCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHCCCCCCCCEEEEEEECCCHHHH
LSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGVGQQLIASA
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEHHCCCCEEHEECCCCHHHCCCHHHHHHHH
LQYTQDLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQL
HHHHHCCCEEEEECCCCHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHH
AEREHYCLRRDAQALAQWNGETLPVDPLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHH
LLQTSELALPALRGRLQKNASDAQLCTTLKLAGRKMLLVRQREEAAHALFALNDVRTERL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHH
RDRITQWQFFH
HHHHHHCCCCC
>Mature Secondary Structure 
AELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALLGDWLWISPQPDAENHC
CCHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCC
SPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWLVLLLPVWEEWENQPDADS
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEEECHHHHCCCCCCCC
LRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSLAHFTPRTDWHPATGAPQPEQ
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCHHH
QQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATDVLAQFAGEK
HHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCCC
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLL
EEEECCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
KFCARFPHLHRFELQQPIRWAQGCPLEKMVSEALVFDDENFTHTPQGNIVISAFEQTLWR
HHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCCCCCCCEEEEHHHHHHHC
SEPETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQYFLQAAGENEIAGALWLVDEGGLSQQ
CCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHCCCCCCCCEEEEEEECCCHHHH
LSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGVGQQLIASA
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEHHCCCCEEHEECCCCHHHCCCHHHHHHHH
LQYTQDLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQL
HHHHHCCCEEEEECCCCHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHH
AEREHYCLRRDAQALAQWNGETLPVDPLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHH
LLQTSELALPALRGRLQKNASDAQLCTTLKLAGRKMLLVRQREEAAHALFALNDVRTERL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHH
RDRITQWQFFH
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]