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The map label for this gene is insG [H]

Identifier: 82544665

GI number: 82544665

Start: 2197768

End: 2198316

Strand: Reverse

Name: insG [H]

Synonym: SBO_2214

Alternate gene names: 82544665

Gene position: 2198316-2197768 (Counterclockwise)

Preceding gene: 82544666

Following gene: 82544664

Centisome position: 48.64

GC content: 50.46

Gene sequence:

>549_bases
ATGGATAAAGGTTATTACTCACTGGGACTGTTAAATGCCCGGAGCCTGGCGGGAGAACACCGCCACTGGATGATACCTCT
CAGAAAAGGAGCGCAATATGAAGAGCTCAGAAAACTGGGTAAAGGCGATCATCTGGTGAAGCTGAAAACCAGCCCGCAGG
CACGAAAAAAGTGGCCGGGACTGGGAAATGAAGTGACAGCCCGCCTGCTGACCGTGACGCGCAAAGGAAAAGTCTGCCAT
CTGCTGACGTCGATGACGGACGCCATGCGCTTCCCCGGAGGAGAAATGGCGGATCTGTACAGTCATCGCTGGGAAATTGA
ACTGGGATACAGGGAGATAAAACAGACGATGCAACTGAGCAGGCTGACGCTGAGAAGTAAAAAGCCGGAGCTTGTGGAGC
AAGAGCTGTGGGGTGTCTTACTGGCTTATAATCTGGTGAGATATCAGATGATTAAAATGGCGGAACATCTGAAAGGTTAC
TGGCCGAATCAACTGAGTTTCTCAGAATCATGCGGAATGGTGGGTAATGCTGCCAACTTACTGATTTAG

Upstream 100 bases:

>100_bases
CATCTGCTGACGGCTGCAGCCTTCGGCACGATGAAGAACAGCGAAAAATGAGCTTGCTGAGCAACTTATAGAACAAACCG
GCGATAACACTCTGACGTTA

Downstream 100 bases:

>100_bases
TGTATGATGGTGATTTTAAGGTGCTTGCGTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTAG
TGCGTAACGGCAAAAGCACT

Product: IS4 orf

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MDKGYYSLGLLNARSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCH
LLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGY
WPNQLSFSESCGMVGNAANLLI

Sequences:

>Translated_182_residues
MDKGYYSLGLLNARSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCH
LLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGY
WPNQLSFSESCGMVGNAANLLI
>Mature_182_residues
MDKGYYSLGLLNARSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCH
LLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGY
WPNQLSFSESCGMVGNAANLLI

Specific function: Involved in the transposition of the insertion sequence IS4 [H]

COG id: COG3385

COG function: function code L; FOG: Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1790729, Length=174, Percent_Identity=97.7011494252874, Blast_Score=353, Evalue=6e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559 [H]

Pfam domain/function: PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 20917; Mature: 20917

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKGYYSLGLLNARSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPG
CCCCEEEEECCHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEEECCCHHHHHCCC
LGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLS
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCEEEEECHHHHHHHHHHH
RLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVGNAANL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
LI
CC
>Mature Secondary Structure
MDKGYYSLGLLNARSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPG
CCCCEEEEECCHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEEECCCHHHHHCCC
LGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLS
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCEEEEECHHHHHHHHHHH
RLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVGNAANL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
LI
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6268937; 7610040; 9278503 [H]