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The map label for this gene is ycdW [H]

Identifier: 82544500

GI number: 82544500

Start: 2001861

End: 2002838

Strand: Reverse

Name: ycdW [H]

Synonym: SBO_2035

Alternate gene names: 82544500

Gene position: 2002838-2001861 (Counterclockwise)

Preceding gene: 82544501

Following gene: 82544499

Centisome position: 44.31

GC content: 50.92

Gene sequence:

>978_bases
GTGCGAATAAATTTCGCACAACGCTTTTCGGGAGTCAGTATGGATATCATCTTTTATCACCCAACGTTCGATACCCAATG
GTGGATTGAGGCACTGCGCAAAGCTATTCCTCAGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGATT
ATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGATCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTT
GATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGG
TATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGC
AAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATTGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTG
GGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGG
CGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATA
CCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGT
GGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTTAAAGGCGCAATGTTGGATGTTTTTAA
TCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCC
GTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGAC
CGCGCACGCGGCTACTAA

Upstream 100 bases:

>100_bases
AAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCC
TTTGTAGATCATAACGATAA

Downstream 100 bases:

>100_bases
TAAAGCATCAGGATTCCTGCTATCCTTGGCGGGAATTGAATACAGGAGAGAGTTATGTATCCCGTCGACCTTCATATGCA
TACCGTTGCCAGCACACATG

Product: dehydrogenase

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MRINFAQRFSGVSMDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALVWHPPVEMLAGRDLKAVFALGAGV
DSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHWEDFTIGILGAGVL
GSKVAQSLQTWRFPLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPDGAYLLNLAR
GVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITRPAEAVEYISRTIAQLEKGERVCGQVD
RARGY

Sequences:

>Translated_325_residues
MRINFAQRFSGVSMDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALVWHPPVEMLAGRDLKAVFALGAGV
DSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHWEDFTIGILGAGVL
GSKVAQSLQTWRFPLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPDGAYLLNLAR
GVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITRPAEAVEYISRTIAQLEKGERVCGQVD
RARGY
>Mature_325_residues
MRINFAQRFSGVSMDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALVWHPPVEMLAGRDLKAVFALGAGV
DSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHWEDFTIGILGAGVL
GSKVAQSLQTWRFPLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPDGAYLLNLAR
GVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITRPAEAVEYISRTIAQLEKGERVCGQVD
RARGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=237, Percent_Identity=28.2700421940928, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI23308577, Length=261, Percent_Identity=26.8199233716475, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI87081824, Length=312, Percent_Identity=99.6794871794872, Blast_Score=641, Evalue=0.0,
Organism=Escherichia coli, GI87082289, Length=258, Percent_Identity=27.1317829457364, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=263, Percent_Identity=28.1368821292776, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324964, Length=212, Percent_Identity=25.9433962264151, Blast_Score=73, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324980, Length=178, Percent_Identity=28.0898876404494, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320925, Length=199, Percent_Identity=24.6231155778894, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322116, Length=199, Percent_Identity=24.6231155778894, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324055, Length=179, Percent_Identity=29.0502793296089, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28574286, Length=263, Percent_Identity=30.0380228136882, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24585514, Length=243, Percent_Identity=26.3374485596708, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28574282, Length=243, Percent_Identity=26.3374485596708, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI45552429, Length=243, Percent_Identity=26.3374485596708, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28574284, Length=243, Percent_Identity=26.3374485596708, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45551003, Length=243, Percent_Identity=26.3374485596708, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24585516, Length=235, Percent_Identity=27.6595744680851, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921140, Length=170, Percent_Identity=31.7647058823529, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 36869; Mature: 36869

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRINFAQRFSGVSMDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALVWHP
CCCCHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEEECC
PVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEYAVSQV
CHHHHCCCCHHHHHHHHCCHHHHHHHHHHCHHHCCCCCCEEEECCCCCHHHHHHHHHHHH
LHWFRRFDDYRIQQNSSHWQPLPEYHWEDFTIGILGAGVLGSKVAQSLQTWRFPLRCWSR
HHHHHHCCCHHCCCCCCCCCCCCCCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHH
TRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPDGAYLLNLAR
HHHCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
GVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITRPAEAVE
CCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEECCCHHHHCCHHHHHH
YISRTIAQLEKGERVCGQVDRARGY
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRINFAQRFSGVSMDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALVWHP
CCCCHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEEECC
PVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEYAVSQV
CHHHHCCCCHHHHHHHHCCHHHHHHHHHHCHHHCCCCCCEEEECCCCCHHHHHHHHHHHH
LHWFRRFDDYRIQQNSSHWQPLPEYHWEDFTIGILGAGVLGSKVAQSLQTWRFPLRCWSR
HHHHHHCCCHHCCCCCCCCCCCCCCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHH
TRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPDGAYLLNLAR
HHHCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
GVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHVAAITRPAEAVE
CCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEECCCHHHHCCHHHHHH
YISRTIAQLEKGERVCGQVDRARGY
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA