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The map label for this gene is cheB

Identifier: 82543637

GI number: 82543637

Start: 1121368

End: 1122306

Strand: Direct

Name: cheB

Synonym: SBO_1117

Alternate gene names: 82543637

Gene position: 1121368-1122306 (Clockwise)

Preceding gene: 82543636

Following gene: 82543638

Centisome position: 24.81

GC content: 52.82

Gene sequence:

>939_bases
ATGAGCAAAATCAGGGTGTTATCTGTCGATGATTCGGCACTGATGCGCCAGATCATGACAGAAATCATCAACAGCCATAG
CGACATGGAAATGGTGGCGACCGCGCCTGATCCGCTGGTCGCGCGTGACTTGATTAAGAAATTCAATCCCGATGTGCTGA
CGCTGGATGTTGAAATGCCGCGGATGGACGGACTGGATTTCCTCGAAAAATTAATGCGTTTGCGTCCAATGCCCGTTGTG
ATGGTTTCTTCCTTGACCGGCAAAGGATCAGAAGTCACGCTGCGCGCGCTGGAGCTGGGGGCAATAGATTTTGTCACCAA
ACCGCAACTGGGTATTCGCGAAGGAATGCTGGCGTATAGCGAAATGATTGCTGAAAAGGTGCGTACGGCAGCAAAGGCGA
GCCTTGCAGCACATAAGCCATTGTCAGCACCGACAACGCTGAAGGCGGGTCCGTTGTTGAGTTCTGAAAAACTGATTGCG
ATTGGTGCTTCAACGGGTGGAACTGAGGCAATTCGTCACGTACTGCAACCGTTGCCGCTTTCCAGCCCGGCATTGTTAAT
TACCCAACATATGCCGCCCGGTTTCACCCGCTCTTTTGCCGACAGACTTAATAAGCTTTGCCAGATCGGGGTTAAAGAAG
CCGAAGACGGAGAACGTGTCTTGCCGGGGCATGCCTATATTGCGCCGGGCGATCGGCATATGGAACTGGCGCGTAGTGGC
GCAAATTATCAAATCAAAATTCACGACGGCCCGGTGGGAATGTTGGCGATGCGTCAGGCGGGGGCATGGACCCTTGCGCA
AAACGAAGCAAGTTGCGTGGTGTTCGGCATGCCGCGCGAGGCCATCAATATGGGTGGTGTCTGCGAAGTGGTCGATCTTA
GCCAGGTAAGCCAGCAAATGTTGGCAAAAATTAGTGCCGGACAGGCGATACGTATTTAA

Upstream 100 bases:

>100_bases
ACGGATTGCTGTTTGCGGGTCACTCTGAAAACTTTAGCCACCTTGAGCGCCGCTTCACGCTGCGTGGTCAGACGGTGTAT
GCGCTAAGTAAGGATTAACG

Downstream 100 bases:

>100_bases
ATCAGGAGTGTGAAATGGCGGATAAAGAACTTAAATTTTTGGTTGTGGATGACTTTTCCACCATGCGACGCATAGTGCGT
AACCTGCTGAAAGAGCTGGG

Product: protein methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDLIKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVV
MVSSLTGKGSEVTLRALELGAIDFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGERVLPGHAYIAPGDRHMELARSG
ANYQIKIHDGPVGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVSQQMLAKISAGQAIRI

Sequences:

>Translated_312_residues
MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDLIKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVV
MVSSLTGKGSEVTLRALELGAIDFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGERVLPGHAYIAPGDRHMELARSG
ANYQIKIHDGPVGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVSQQMLAKISAGQAIRI
>Mature_311_residues
SKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDLIKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVM
VSSLTGKGSEVTLRALELGAIDFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIAI
GASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGERVLPGHAYIAPGDRHMELARSGA
NYQIKIHDGPVGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVSQQMLAKISAGQAIRI

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=349, Percent_Identity=88.5386819484241, Blast_Score=612, Evalue=1e-177,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB_SHIBS (Q322K2)

Other databases:

- EMBL:   CP000036
- RefSeq:   YP_407584.1
- ProteinModelPortal:   Q322K2
- SMR:   Q322K2
- EnsemblBacteria:   EBESCT00000077061
- GeneID:   3779673
- GenomeReviews:   CP000036_GR
- KEGG:   sbo:SBO_1117
- GeneTree:   EBGT00050000010338
- HOGENOM:   HBG705324
- OMA:   FVTKPKL
- PhylomeDB:   Q322K2
- BioCyc:   SBOY300268:SBO_1117-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 33656; Mature: 33525

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 164-164 ACT_SITE 190-190 ACT_SITE 249-249

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDLIKKFNPDVLTLDVEMP
CCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCEEEEEECCC
RMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAIDFVTKPQLGIREGMLAYS
CCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEECCEEEECCCCCCHHHHHHHHH
EMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIAIGASTGGTEAIRHVLQPLPL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCC
SSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGERVLPGHAYIAPGDRHMELARSG
CCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEECCCCEEECCCHHHHHHHHCC
ANYQIKIHDGPVGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVSQQM
CCEEEEEECCCCCEEEHHCCCCEEEECCCCCEEEEECCHHHHCCCCEEEEHHHHHHHHHH
LAKISAGQAIRI
HHHHCCCCEECC
>Mature Secondary Structure 
SKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDLIKKFNPDVLTLDVEMP
CCEEEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCEEEEEECCC
RMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAIDFVTKPQLGIREGMLAYS
CCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEECCEEEECCCCCCHHHHHHHHH
EMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIAIGASTGGTEAIRHVLQPLPL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCC
SSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGERVLPGHAYIAPGDRHMELARSG
CCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEECCCCEEECCCHHHHHHHHCC
ANYQIKIHDGPVGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVSQQM
CCEEEEEECCCCCEEEHHCCCCEEEECCCCCEEEEECCHHHHCCCCEEEEHHHHHHHHHH
LAKISAGQAIRI
HHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA