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The map label for this gene is pbpG [H]

Identifier: 82543546

GI number: 82543546

Start: 1027857

End: 1028798

Strand: Direct

Name: pbpG [H]

Synonym: SBO_1010

Alternate gene names: 82543546

Gene position: 1027857-1028798 (Clockwise)

Preceding gene: 82543545

Following gene: 82543548

Centisome position: 22.74

GC content: 50.32

Gene sequence:

>942_bases
ATGCTCATCATGCCGAAATTTCGAGTTTCTTTATTTAGCCTGGCCCTGATGCTGGCTGTGCCTTTGGCACCGCAGGCCGT
TGCGAAAACGGCAGCCGCTATCACCGCTTCACAACCTGAAATTGCCTCCGGTAGCGCGATGATTGTTGATCTGAATACCA
ACAAAGTGATCTACTCGAACCACCCGGATCTGGTGCGTCCGATTGCGTCTATCAGCAAATTAATGACCGCGATGGTTGTG
CTGGATGCACGACTGCCGCTGGATGAAAAACTAAAAGTGGATATCAGCCAGACGCCGGAGATGAAAGGGGTCTATTCGCG
CGTACGACTGAATAGCGAAATCAGCCGTAAAGATATGCTGTTGCTGGCGCTGATGTCTTCAGAAAACCGCGCGGCGGCAA
GCCTTGCACACCATTATCCCGGTGGTTACAAAGCCTTTATTAAGGCAATGAATGCGAAAGCGAAATCGCTCGGAATGAAC
AACACGCGCTTTGTTGAACCTACCGGATTGTCGGTGCATAACGTTTCAACTGCCCGTGACTTAACCAAACTGCTCATTGC
CAGCAAACAATATCCGTTGATCGGGCAGTTAAGTACTACCCGGGAAGATATGGCAACCTTCTCTAACCCGACGTATACGC
TGCCGTTCCGCAATACTAACCATCTGGTGTATCGCGATAACTGGAATATTCAGTTAACCAAAACCGGTTTTACCAATGCG
GCGGGCCATTGTCTGGTGATGCGTACGGTTATCAATAATAAACCGGTGGCGCTGGTAGTGATGGATGCGTTTGGCAAATA
TACCCATTTTGCCGATGCCAGCCGCCTGCGTACCTGGATTGAAACCGGTAAAGTGATGCCTGTTCCAGCAGCAGCGTTGA
GCTATAAAAAACAAAAAGCCGCCCAAATGGCGGCGGCGGGGCAGACGGCACAGAACGATTAA

Upstream 100 bases:

>100_bases
CTTTGACGATCGCACTTTTTGCTCGTTATGATGGCGTCGCTAGCCTCAGTAAATCCTTACTCCGGCGGTGCGCAACCTGT
GCGCGTGAACCACTATCTGA

Downstream 100 bases:

>100_bases
GTGAATGACGGATGGCGATACTATGCCATCCGTAATTTTTACTCCACTTCCTGCCAGTTTTTCCGCTTACTGGTTTTACC
GATCCCCGGATTCATACTGT

Product: D-alanyl-D-alanine endopeptidase

Products: NA

Alternate protein names: DD-endopeptidase; Penicillin-binding protein 7; PBP-7 [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MLIMPKFRVSLFSLALMLAVPLAPQAVAKTAAAITASQPEIASGSAMIVDLNTNKVIYSNHPDLVRPIASISKLMTAMVV
LDARLPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMN
NTRFVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNA
AGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND

Sequences:

>Translated_313_residues
MLIMPKFRVSLFSLALMLAVPLAPQAVAKTAAAITASQPEIASGSAMIVDLNTNKVIYSNHPDLVRPIASISKLMTAMVV
LDARLPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMN
NTRFVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNA
AGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND
>Mature_313_residues
MLIMPKFRVSLFSLALMLAVPLAPQAVAKTAAAITASQPEIASGSAMIVDLNTNKVIYSNHPDLVRPIASISKLMTAMVV
LDARLPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMN
NTRFVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNA
AGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND

Specific function: Cell wall formation. May play a specialized role in remodeling the cell wall. Specifically hydrolyze the DD- diaminopimelate-alanine bonds in high-molecular-mass murein sacculi [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082054, Length=310, Percent_Identity=99.3548387096774, Blast_Score=635, Evalue=0.0,
Organism=Escherichia coli, GI1787062, Length=270, Percent_Identity=28.1481481481481, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87082022, Length=287, Percent_Identity=25.7839721254355, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786851, Length=264, Percent_Identity=26.5151515151515, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR018044
- InterPro:   IPR001967 [H]

Pfam domain/function: PF00768 Peptidase_S11 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 34223; Mature: 34223

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIMPKFRVSLFSLALMLAVPLAPQAVAKTAAAITASQPEIASGSAMIVDLNTNKVIYSN
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEECC
HPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDML
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCHHHEE
LLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTRFVEPTGLSVHNVSTARD
EEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHCCCCCCEEECCCCCEEECCHHHHH
LTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNA
HHHHHHHCCCCCEEECCCCCHHHHHHCCCCEEEEECCCCCEEEEECCCEEEEEECCCCCC
AGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKA
CCCEEEEEEHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
AQMAAAGQTAQND
HHHHHCCCCCCCC
>Mature Secondary Structure
MLIMPKFRVSLFSLALMLAVPLAPQAVAKTAAAITASQPEIASGSAMIVDLNTNKVIYSN
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEECC
HPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDML
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCHHHEE
LLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTRFVEPTGLSVHNVSTARD
EEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHCCCCCCEEECCCCCEEECCHHHHH
LTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNA
HHHHHHHCCCCCEEECCCCCHHHHHHCCCCEEEEECCCCCEEEEECCCEEEEEECCCCCC
AGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKA
CCCEEEEEEHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
AQMAAAGQTAQND
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 6386470; 7721700; 7925376 [H]