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The map label for this gene is speF [H]

Identifier: 82543125

GI number: 82543125

Start: 569168

End: 571366

Strand: Reverse

Name: speF [H]

Synonym: SBO_0554

Alternate gene names: 82543125

Gene position: 571366-569168 (Counterclockwise)

Preceding gene: 82543127

Following gene: 161984971

Centisome position: 12.64

GC content: 49.34

Gene sequence:

>2199_bases
ATGTCAAAATTAAAAATTGCGGTTAGTGATTCTTGCCCGGACTGTTTTACCACGCAGCGAGAATGTATCTACATTAATGA
AAGTCGTAATATCGATGTGGCGGCAATCGTTTTATCGCTCAACGATGTTACATGCGGAAAACTCGATGAAATCGATGCCA
CGGGTTATGGCATCCCGGTATTTATTGCTACTGAAAATCAAGAACGTGTACCCGCAGAGTATTTGCCCTGTATTTCGGGT
GTCTTTGAGAATTGCGAATCGCGACGAGAATTTTATGGTCGTCAGTTAGAAACCGCTGCCAGCCATTATGAAACTCAACT
GCGCCCACCTTTCTTCCGCGCACTGGTCGATTATGTCAATCAAGGTAACAGCGCGTTTGATTGCCCTGGTCATCAGGGCG
GCGAATTTTTCCGTCGCCATCCGGCGGGGAATCAGTTTGTGGAATACTTTGGTGAGGCGCTGTTCCGTTCCGACTTGTGC
AACGCAGACGTAGCAATGGGTGATCTGCTGATTCACGAAGGCGCGCCATGCATTGCACAGCAACATGCGGCAAAAGTGTT
CAATGCCGATAAAACATACTTCGTTCTAAATGGCACTTCATCTTCTAACAAAGTGGTTTTAAACGCCCTGCTGACACCGG
GTGATCTGGTGCTGTTTGACCGCAATAACCACAAATCTAACCACCACGGAGCGTTGCTACAGGCTGGTGCAACACCGGTT
TATCTGGAAACCGCACGTAACCCGTATGGCTTTATCGGTGGCATTGATGCGCACTGTTTTGAAGAAAGTTACCTGCGTGA
GCTGATCGCGGAAGTCGCACCGCAGCGGACAAAAGAGGCTCGTCCTTTCCGCCTCGCCGTTATTCAGCTAGGCACCTACG
ACGGTACGATTTATAACGCCCGCCAGGTGGTGGATAAAATTGGTCATCTGTGTGACTACATTCTGTTTGACTCCGCATGG
GTCGGCTATGAGCAATTTATCCCGATGATGGCTGACTGTTCACCGCTGTTGCTGGATCTTAATGAGAACGATCCGGGCAT
TCTGGTTACGCAATCTGTGCATAAACAGCAGGCTGGTTTTTCTCAAACTTCACAAATCCATAAAAAAGACAGCCACATCA
AAGGGCAACAGCGTTATGTACCGCACAAACGCATGAACAACGCCTTTATGATGCATGCCTCCACCAGCCCGTTCTATCCG
CTGTTTGCCGCACTGGATATCAACGCCAAAATGCATGAAGGTGTCAGCGGTCGTAATATGTGGATGGATTGTGTGGTAAA
TGGCATTAATGCCCGCAAGCTGATCCTCGATAACTGTCAGCATATTCGTCCGTTCGTACCTGAACTAGTGGATGGTAAAC
CCTGGCAGTCGTATGAAACAGCGCAAATTGCGGTTGATCTGCGCTTCTTCCAGTTTGTACCAGGGGAACACTGGCACTCT
TTTGAAGGCTATGCAGAGAATCAATACTTTGTCGATCCATGCAAACTGTTGCTGACAACCCCAGGTTTTGATGCACGTAA
CGGCGAATATGAAGCGTTCGGTGTACCCGCGACGATTCTTGCTAACTTCCTGCGCGAAAATGGCGTAGTGCCGGAAAAAT
GCGATCTTAACTCCATCCTCTTCCTGCTGACTCCGGCAGAAGATATGGCCAAACTTCAGCAACTTGTTGCCCTGCTGGTA
CGCTTCGAAAAACTGCTTGAGTCCGACGCGCCATTAGCAGAAGTGCTGCCTTCCATCTACAAACAGCATGAAGAGCGCTA
CGCCGGTTATACCCTTCGTCAGTTATGCCAGGAAATGCATGATTTGTATGCCCGTCACAACGTGAAACAACCGCAAAAAG
AGATGTTCCGTAAGGAACACTTCCCACGCGTAAGCATGAATCCACAAGAAGCCAACTACGCCTATTCACGCGGTGAAGTG
GAGCTGGTTCGTCTGCCGGATGCCGAAGGCCGTATCGCTGCCGAAGGTGCGCTTCCTTATCCTCCGGGTGTGCTGTGTGT
TGTTCCGGGTGAAATCTGGGGTGGTGCTGTTTTGCGTTACTTCAGCGCTCTGGAAGAAGGGATCAACCTGCTGCCAGGTT
TTGCACCGGAGCTGCAGGGTGTCTATATCGAAGAACATGATGGTCGTAAGCAAGTTTGGTGCTATGTCATCAAGCCTCGT
GATGCGCAAAGCACCCTGTTGAAAGGGGAAAAATTATGA

Upstream 100 bases:

>100_bases
ATTTATTTATCAGCGGGGTAACTTTGCTTTTTTCCGGCACGATCGATTTCTCATTCGAGAAATTGAGGACCTGCTATTAC
CTAAAATAAAGAGATGAAAA

Downstream 100 bases:

>100_bases
GTCAGGCTAAATCGAACAAAATGGGCGTCGTTCAGTTAACCATACTGACGATGGTCAACATGATGGGCTCCGGTATCATC
ATGCTGCCGACAAAGCTTGC

Product: ornithine decarboxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 732; Mature: 731

Protein sequence:

>732_residues
MSKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVTCGKLDEIDATGYGIPVFIATENQERVPAEYLPCISG
VFENCESRREFYGRQLETAASHYETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRSDLC
NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHKSNHHGALLQAGATPV
YLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQRTKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAW
VGYEQFIPMMADCSPLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFYP
LFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQSYETAQIAVDLRFFQFVPGEHWHS
FEGYAENQYFVDPCKLLLTTPGFDARNGEYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLV
RFEKLLESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQPQKEMFRKEHFPRVSMNPQEANYAYSRGEV
ELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFSALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPR
DAQSTLLKGEKL

Sequences:

>Translated_732_residues
MSKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVTCGKLDEIDATGYGIPVFIATENQERVPAEYLPCISG
VFENCESRREFYGRQLETAASHYETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRSDLC
NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHKSNHHGALLQAGATPV
YLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQRTKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAW
VGYEQFIPMMADCSPLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFYP
LFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQSYETAQIAVDLRFFQFVPGEHWHS
FEGYAENQYFVDPCKLLLTTPGFDARNGEYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLV
RFEKLLESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQPQKEMFRKEHFPRVSMNPQEANYAYSRGEV
ELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFSALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPR
DAQSTLLKGEKL
>Mature_731_residues
SKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVTCGKLDEIDATGYGIPVFIATENQERVPAEYLPCISGV
FENCESRREFYGRQLETAASHYETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRSDLCN
ADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHKSNHHGALLQAGATPVY
LETARNPYGFIGGIDAHCFEESYLRELIAEVAPQRTKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWV
GYEQFIPMMADCSPLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFYPL
FAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQSYETAQIAVDLRFFQFVPGEHWHSF
EGYAENQYFVDPCKLLLTTPGFDARNGEYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVR
FEKLLESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQPQKEMFRKEHFPRVSMNPQEANYAYSRGEVE
LVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFSALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRD
AQSTLLKGEKL

Specific function: Spermidine biosynthesis from ornithine. [C]

COG id: COG1982

COG function: function code E; Arginine/lysine/ornithine decarboxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Orn/Lys/Arg decarboxylase class-I family [H]

Homologues:

Organism=Escherichia coli, GI1786909, Length=732, Percent_Identity=99.0437158469945, Blast_Score=1512, Evalue=0.0,
Organism=Escherichia coli, GI87082193, Length=719, Percent_Identity=66.4812239221141, Blast_Score=1004, Evalue=0.0,
Organism=Escherichia coli, GI221142684, Length=678, Percent_Identity=31.7109144542773, Blast_Score=340, Evalue=2e-94,
Organism=Escherichia coli, GI1790573, Length=636, Percent_Identity=33.3333333333333, Blast_Score=309, Evalue=5e-85,
Organism=Escherichia coli, GI1786384, Length=692, Percent_Identity=31.0693641618497, Blast_Score=299, Evalue=5e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR005308
- InterPro:   IPR011193
- InterPro:   IPR000310
- InterPro:   IPR008286
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01276 OKR_DC_1; PF03711 OKR_DC_1_C; PF03709 OKR_DC_1_N [H]

EC number: =4.1.1.17 [H]

Molecular weight: Translated: 82403; Mature: 82271

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00703 OKR_DC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVTCGKLDEIDATGYGIPV
CCCEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEE
FIATENQERVPAEYLPCISGVFENCESRREFYGRQLETAASHYETQLRPPFFRALVDYVN
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRSDLCNADVAMGDLLIHEGAPCIAQ
CCCCEECCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHEEEECCCCHHHH
QHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHKSNHHGALLQAGATPV
HHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCEEECCCCEE
YLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQRTKEARPFRLAVIQLGTYDGTIYNA
EEEECCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCHHH
RQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCSPLLLDLNENDPGILVTQSVHKQQAGF
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEHHHHHHHCCC
SQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFYPLFAALDINAKMHEGVSGRNM
HHHHHHHHHHHCCCCCHHCCCHHHCCCEEEEEECCCCCCEEEEEEECCCHHHCCCCCCCH
WMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQSYETAQIAVDLRFFQFVPGEHWHS
HHHHHHCCCCHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEHCCCCCCCCCC
FEGYAENQYFVDPCKLLLTTPGFDARNGEYEAFGVPATILANFLRENGVVPEKCDLNSIL
CCCCCCCCEEECCEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCEE
FLLTPAEDMAKLQQLVALLVRFEKLLESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMH
EEEECHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
DLYARHNVKQPQKEMFRKEHFPRVSMNPQEANYAYSRGEVELVRLPDAEGRIAAEGALPY
HHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCEECCCCEEEEECCCCCCCEEECCCCCC
PPGVLCVVPGEIWGGAVLRYFSALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPR
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEEEECCC
DAQSTLLKGEKL
CHHHHHHCCCCC
>Mature Secondary Structure 
SKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVTCGKLDEIDATGYGIPV
CCEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEE
FIATENQERVPAEYLPCISGVFENCESRREFYGRQLETAASHYETQLRPPFFRALVDYVN
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRSDLCNADVAMGDLLIHEGAPCIAQ
CCCCEECCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHEEEECCCCHHHH
QHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHKSNHHGALLQAGATPV
HHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCEEECCCCEE
YLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQRTKEARPFRLAVIQLGTYDGTIYNA
EEEECCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCHHH
RQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCSPLLLDLNENDPGILVTQSVHKQQAGF
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEHHHHHHHCCC
SQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFYPLFAALDINAKMHEGVSGRNM
HHHHHHHHHHHCCCCCHHCCCHHHCCCEEEEEECCCCCCEEEEEEECCCHHHCCCCCCCH
WMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQSYETAQIAVDLRFFQFVPGEHWHS
HHHHHHCCCCHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEHCCCCCCCCCC
FEGYAENQYFVDPCKLLLTTPGFDARNGEYEAFGVPATILANFLRENGVVPEKCDLNSIL
CCCCCCCCEEECCEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCEE
FLLTPAEDMAKLQQLVALLVRFEKLLESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMH
EEEECHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
DLYARHNVKQPQKEMFRKEHFPRVSMNPQEANYAYSRGEVELVRLPDAEGRIAAEGALPY
HHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCEECCCCEEEEECCCCCCCEEECCCCCC
PPGVLCVVPGEIWGGAVLRYFSALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPR
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEEEECCC
DAQSTLLKGEKL
CHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939141; 8905232; 9278503 [H]