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The map label for this gene is ybeJ [H]

Identifier: 82543098

GI number: 82543098

Start: 536130

End: 537038

Strand: Reverse

Name: ybeJ [H]

Synonym: SBO_0518

Alternate gene names: 82543098

Gene position: 537038-536130 (Counterclockwise)

Preceding gene: 82543101

Following gene: 82543097

Centisome position: 11.88

GC content: 48.4

Gene sequence:

>909_bases
ATGCAATTACGTAAACCTGCCACAGCAATCCTCGCCCTGGCGCTTTCCGCAGGACTGGCACAGGCAGATGACGCCGCCCC
GGCAGCAGGCAGTACGCTGGACAAAATCGCCAAAAACGGTGTGATTGTCGTCGGTCACCGTGAATCTTCAGTACCTTTCT
CTTATTACGACAATCAGCAAAAAGTGGTGGGTTACTCGCAGGATTACTCCAACGCCATTGTTGAAGCAGTGAAAAAGAAA
CTCAACAAACCGGACTTGCAGGTAAAACTGATTCCGATTACCTCACAAAACCGTATTCCACTGCTGCAAAACGGCACTTT
CGATTTTGAATGTGGTTCTACCACCAACAACGTCGAACGCCAAAAACAGGCGGCTTTCTCTGATACTATTTTCGTGGTCG
GTACGCGCCTGCTGACCAAAAAGGGCGGCGATATCAAAGATTTTGCCGACCTGAAAGACAAAGCCGTAGTCGTCACTTCC
GGCACTACCTCTGAAGTTTTGCTCAACAAACTGAATGAAGAGCAAAAAATGAATATGCGCATCATCAGCGCCAAAGATCA
CGGTGACTCTTTCCGCACCCTGGAAAGCGGTCGTGCCGTTGCCTTTATGATGGATGATGCCCTGCTGGCCGGTGAACGTG
CGAAAGCGAAGAAACCAGACAACTGGGATATCGTCGGCAAGCCGCAGTCTCAGGAAGCCTACGGTTGCATGTTGCGTAAA
GATGATCCGCAGTTCAAAAAGCTGATGGATGACACTATCGCTCAGGTGCAGACTTCCGGTGAAGCGGAAAAATGGTTTGA
TAAATGGTTCAAAAATCCAATTCCGCCGAAAAACCTGAACATGAATTTCGAACTGTCAGACGAAATGAAAGCACTGTTCA
AAGAACCGAATGACAAAGCACTGAACTAA

Upstream 100 bases:

>100_bases
ACAGGCACAACACTGCACAATAAAGTTGCAGACGATAACAACACAAACACTCACAACGGGTATCCATGCGTTCTTAACGC
AGAAGATAAAGGAGTTGGAT

Downstream 100 bases:

>100_bases
TTACAAGAACCAGGGGCGGAAATTCCAGCCCTCTCGATTGTTACGTAGCACGGACAGACTATACGCCTGATGGTCGTTCC
CCATCGGGCCTGAAAACCGC

Product: glutamate and aspartate transporter subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVVGHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKK
LNKPDLQVKLIPITSQNRIPLLQNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKKPDNWDIVGKPQSQEAYGCMLRK
DDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPPKNLNMNFELSDEMKALFKEPNDKALN

Sequences:

>Translated_302_residues
MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVVGHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKK
LNKPDLQVKLIPITSQNRIPLLQNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKKPDNWDIVGKPQSQEAYGCMLRK
DDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPPKNLNMNFELSDEMKALFKEPNDKALN
>Mature_302_residues
MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVVGHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKK
LNKPDLQVKLIPITSQNRIPLLQNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKKPDNWDIVGKPQSQEAYGCMLRK
DDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPPKNLNMNFELSDEMKALFKEPNDKALN

Specific function: Part of the binding-protein-dependent transport system for glutamate and aspartate. Binds to both aspartate and glutamate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1786876, Length=302, Percent_Identity=99.3377483443709, Blast_Score=618, Evalue=1e-178,
Organism=Escherichia coli, GI1788228, Length=272, Percent_Identity=26.1029411764706, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788649, Length=272, Percent_Identity=26.8382352941176, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788648, Length=237, Percent_Identity=25.3164556962025, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 33407; Mature: 33407

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVVGHRESSVPFSYYDNQQ
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCEEEEECCCCCCCCCEECCCC
KVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLLQNGTFDFECGSTTNNVER
EEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEECCCEEEECCCCCCHHHH
QKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTSGTTSEVLLNKLNEEQKMNMR
HHHHHHHCCCEEEHHHHHHCCCCCHHHHHCCCCCEEEEECCCHHHHHHHHHCHHHHCCEE
IISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKKPDNWDIVGKPQSQEAYGCMLRK
EEEECCCCCHHHHHHCCCEEEEEECCHHHCCCCCCCCCCCCCCEECCCCCCHHHCEEECC
DDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPPKNLNMNFELSDEMKALFKEPNDKA
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHCCCCCCC
LN
CC
>Mature Secondary Structure
MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVVGHRESSVPFSYYDNQQ
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCEEEEECCCCCCCCCEECCCC
KVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLLQNGTFDFECGSTTNNVER
EEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEECCCEEEECCCCCCHHHH
QKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTSGTTSEVLLNKLNEEQKMNMR
HHHHHHHCCCEEEHHHHHHCCCCCHHHHHCCCCCEEEEECCCHHHHHHHHHCHHHHCCEE
IISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKKPDNWDIVGKPQSQEAYGCMLRK
EEEECCCCCHHHHHHCCCEEEEEECCHHHCCCCCCCCCCCCCCEECCCCCCHHHCEEECC
DDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPPKNLNMNFELSDEMKALFKEPNDKA
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHCCCCCCC
LN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123; 1091635; 1091636 [H]