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The map label for this gene is ybaC [H]

Identifier: 82542969

GI number: 82542969

Start: 392160

End: 393119

Strand: Reverse

Name: ybaC [H]

Synonym: SBO_0376

Alternate gene names: 82542969

Gene position: 393119-392160 (Counterclockwise)

Preceding gene: 82542971

Following gene: 82542960

Centisome position: 8.7

GC content: 51.46

Gene sequence:

>960_bases
ATGAAGCCGGAAAACAAACTACCTGTTCTGGACCTTATTTCTGCTGAAATGAAGACCGTTGTGAATACTCTTCAGCCGGA
TTTACCGTCCTGGCCCGCAACGGGAACGATTGCTGAGCAACGACAGTATTACACGCTTGAGCGCCGATTCTGGAATGCGG
GCGCTCCAGAAATGGCAACCAGAGCTTACATGGTTCCAACAAAATATGGGCAGGTGGAAACACGTCTCTTTTGTCCGCAG
CCAGATAGCCCGGCGACGCTATTTTATTTGCATGGAGGCGGTTTTATTCTCGGCAATCTCGATACCCACGATCGCATCAT
GCGCCTGCTGGCAAGCTACAGCCAATGTACGGTGATTGGTATTGATTACCCCCTTTCACCTGAAGCGCGTTTTCCGCAAG
CGATAGAGGAAATTGTGGCTGCTTGTTGTTATTTCCACCAGCAGGCGGAGGATTATCAAATCAATATGTCCCGCATTGGC
TTTGCCGGTGATTCCGCAGGTGCCATGCTGGCGCTCGCCAGTGCGTTGTGGTTGCGTGATAAACAGATCGATTGCGGTAA
AATTGTGGGTGTTTTGCTGTGGTATGGGCTTTACGGATTACGGGATTCCGTGACTCGTCGTCTGTTGGGCGGTGTCTGGG
ATGGCTTAACGCAACAGGATTTGCAGATGTATGAAGAGGCGTATTTAAGCAACGACGCGGACCGCGAGTCACCGTATTAC
TGTCTGTTTAATAATGATCTCACTCGCGAAGTTCCGCCCTGTTTTATTGCCGGGGCGGAGTTCGATCCGCTGCTGGATGA
CAGCCGTCTGCTTTACCAGACGTTAGCGGCGCATCAGCAGCCCTGTGAGTTCAAACTCTACCCAGGTACGCTGCATGCCT
TTTTGCATTATTCACGAATGATGAAAACCGCCGACGAGGCTCTTCGCGACGGCGCTCAGTTCTTTACCGCTCAGCTTTAG

Upstream 100 bases:

>100_bases
GGGAGCGCGCGAGACGCAGCCAGTTTCAATATCAGCAGGCGGGATCGCATTTTCATGGCAGTGGTTCCTTACAATGACGT
AATTTGAAAGGAGTTTTTGT

Downstream 100 bases:

>100_bases
CGATACGCGGCAACAAGATTAGCCATCATTTCGATATATTCTGGCGTGGCATTAAGCGCTGGAATATATTCATATTTTTT
CCCGCCGGCACCGAGGAAGA

Product: acetyl esterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYYTLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQ
PDSPATLFYLHGGGFILGNLDTHDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
FAGDSAGAMLALASALWLRDKQIDCGKIVGVLLWYGLYGLRDSVTRRLLGGVWDGLTQQDLQMYEEAYLSNDADRESPYY
CLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLAAHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL

Sequences:

>Translated_319_residues
MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYYTLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQ
PDSPATLFYLHGGGFILGNLDTHDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
FAGDSAGAMLALASALWLRDKQIDCGKIVGVLLWYGLYGLRDSVTRRLLGGVWDGLTQQDLQMYEEAYLSNDADRESPYY
CLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLAAHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL
>Mature_319_residues
MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYYTLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQ
PDSPATLFYLHGGGFILGNLDTHDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
FAGDSAGAMLALASALWLRDKQIDCGKIVGVLLWYGLYGLRDSVTRRLLGGVWDGLTQQDLQMYEEAYLSNDADRESPYY
CLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLAAHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=132, Percent_Identity=32.5757575757576, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786682, Length=319, Percent_Identity=98.7460815047022, Blast_Score=657, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540028, Length=142, Percent_Identity=32.3943661971831, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71996133, Length=266, Percent_Identity=24.812030075188, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI72001146, Length=130, Percent_Identity=31.5384615384615, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24656084, Length=98, Percent_Identity=39.7959183673469, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24656076, Length=98, Percent_Identity=39.7959183673469, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI20130169, Length=98, Percent_Identity=39.7959183673469, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.-

Molecular weight: Translated: 36062; Mature: 36062

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS01173 LIPASE_GDXG_HIS ; PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYYTLERRFWNAGAPEMAT
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
RAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDTHDRIMRLLASYSQCTVIG
EEEECCCCCCCEEEEEECCCCCCCCEEEEEECCEEEEECCCHHHHHHHHHHCCCCEEEEE
IDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIGFAGDSAGAMLALASALWLRD
ECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCHHHHHHHHHHHHCC
KQIDCGKIVGVLLWYGLYGLRDSVTRRLLGGVWDGLTQQDLQMYEEAYLSNDADRESPYY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEE
CLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLAAHQQPCEFKLYPGTLHAFLHYSRM
EEECCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHH
MKTADEALRDGAQFFTAQL
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYYTLERRFWNAGAPEMAT
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
RAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDTHDRIMRLLASYSQCTVIG
EEEECCCCCCCEEEEEECCCCCCCCEEEEEECCEEEEECCCHHHHHHHHHHCCCCEEEEE
IDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIGFAGDSAGAMLALASALWLRD
ECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCHHHHHHHHHHHHCC
KQIDCGKIVGVLLWYGLYGLRDSVTRRLLGGVWDGLTQQDLQMYEEAYLSNDADRESPYY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEE
CLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLAAHQQPCEFKLYPGTLHAFLHYSRM
EEECCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHH
MKTADEALRDGAQFFTAQL
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA