Click here to switch to the map view.

The map label for this gene is priC [H]

Identifier: 82542960

GI number: 82542960

Start: 383596

End: 384123

Strand: Reverse

Name: priC [H]

Synonym: SBO_0367

Alternate gene names: 82542960

Gene position: 384123-383596 (Counterclockwise)

Preceding gene: 82542969

Following gene: 82542959

Centisome position: 8.5

GC content: 59.09

Gene sequence:

>528_bases
GTGAAAACCGCCCTGCTGCTGGAAAAACTGGAAGGACAGCTCGCTACGCTGCGTCAGCGTTGTGCCCCGGTAGCGCAATT
TGCCACGCTAAGTGCCCGTTTTGACAGACACCTTTTTCAAACCCGTACGACAACACTGCAGGCTTGTCTCGATGAGGCTG
GCGATAATCTGGCTGCGCTTCGTCATGCAGTTGAGCAGCAACAGCTGCCGCAAGTGGCCTGGCTGGCGGAACATCTGGCG
GCACAACTGGAAGCCATCGCGCGTGAAGCCTCCGCCTGGTCATTGCGCGAGTGGGACAGTGCGCCACCGAAAATTGCCCG
CTGGCAGCGTAAACGTATTCAGCATCAGGATTTTGAGCGGCGGCTACGTGAGATGGTTGCCGAACGCAGAGCCCGCCTGG
CGCGGGTGACCGATCTCGTGGAACAGCAAACGCTGCATCGTGAAGTGGAAGCCTATGAAGCGCGCCTGGCACGCTGCCGC
CATGCGCTGGAAAAAATCGAAAACAGGCTAGCGCGCTTAACCCGCTAG

Upstream 100 bases:

>100_bases
AGCCAATGATGATTAAAATGATTCGTTGCATATTGAGTGTTGTCAGCTTACAGAGTGGCTACTTTAGCATAACAATTATC
ATTTTCATTGAGGTCTTATC

Downstream 100 bases:

>100_bases
CAATGGAGAGAGTATGTCACTGGAAAATGCCCCTGACGATGTCAAACTGGCTGTCGATTTGATTGTGCTGCTGGAAGAAA
ATCAGATCCCAGCCCGCACC

Product: primosomal replication protein N''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRTTTLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLA
AQLEAIAREASAWSLREWDSAPPKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
HALEKIENRLARLTR

Sequences:

>Translated_175_residues
MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRTTTLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLA
AQLEAIAREASAWSLREWDSAPPKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
HALEKIENRLARLTR
>Mature_175_residues
MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRTTTLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLA
AQLEAIAREASAWSLREWDSAPPKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
HALEKIENRLARLTR

Specific function: PriA recognizes a specific hairpin sequence on bacteriophage phi X174 ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase [H]

COG id: COG3923

COG function: function code L; Primosomal replication protein N''

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786673, Length=175, Percent_Identity=98.8571428571429, Blast_Score=338, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010890 [H]

Pfam domain/function: PF07445 priB_priC [H]

EC number: NA

Molecular weight: Translated: 20390; Mature: 20390

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRTTTLQACLDEAGDNLAAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAPPKIARWQRKRIQHQDFER
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCRHALEKIENRLARLTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRTTTLQACLDEAGDNLAAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAPPKIARWQRKRIQHQDFER
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCRHALEKIENRLARLTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1856227; 9278503 [H]