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The map label for this gene is acnB [H]

Identifier: 78780178

GI number: 78780178

Start: 1686792

End: 1689365

Strand: Direct

Name: acnB [H]

Synonym: PMT9312_1793

Alternate gene names: 78780178

Gene position: 1686792-1689365 (Clockwise)

Preceding gene: 78780176

Following gene: 78780179

Centisome position: 98.69

GC content: 34.46

Gene sequence:

>2574_bases
ATGAAGAATTTGGAAACATTGCTAAAAGATTATGCAGATCACGTAGCTGAAAGAGCTACCAAGGGTATACCTCCTTTACC
TTTAAATGCTGAGCAAACAAATTGTATTACAAAATTATTGGAACAAGATAATACTTATGATTCATCTTATTTACTTGATT
TACTAATAAATAGAGTACCACCAGGAGTTGATGAGGCTGCTTATGTAAAAGCAAGCTGGCTTACGGCTATTGTTAATTCC
GAAAAATATTGCAAATCAATCAATCCCGAAAAAGCAATTGAAATACTAGGAACAATGATAGGCGGATACAATGTAAATTC
TTTAGTTGAAATACTTAAAGGAGAAAATAGTTTACTTGCTAAAAAAGCGGCAGAAGTTTTAAAAAATATTATTCTTGTTT
ACGACTCAGCTAATGAAATTTATGAATTATCTCAAAATAATATTTATGCAAAAGAGGTTGTAAATAGTTGGGCAAATGCA
GAATGGTTCAAAAATAAAAAAGTTCTGGAGAAAGAGATTACTTGTTTAGTATTTAAAGTTGACGGTGAGACAAACACAGA
CGACTTATCTCCGGCTGTACATGCAACAACACGCCCGGATATTCCGATGCATGCGTTAGCTATGTTGGAATTTAAAATAC
CTGATGGACTAAAGATTCTTGATAATTTAAAAAAACAAAATTTACCGATAGCTTATGTTGGAGATGTTGTTGGAACAGGG
AGTTCTAGAAAATCTGCTATTAATTCACTCATTTGGCATATAGGAGAAGATATCGCTTATGTTCCAAACAAAAAAACAGG
TGGAATAATAATTGGCAACAAAATAGCCCCAATTTTCTTTAATACTGCACAAGATTCAGGTGCTTTACCTATAGAAGCTG
ACGTATCTCATATGAAAACAGGAGATGTTATTAAAATATATCCTTATAAAGGCATTATTAAAAAAATTGAACAAGACTCG
AATACTGAAGAATTAATAAGCAAATTCGACTTGTATCCATCAACTCTTACTGATGAAATTCAAGCTGGCGGAAGAATTAA
TCTTATGATTGGAAGATCTCTTACGGACAAAATTAGAACCAAATTAAATTATCAACCAAGTGAAATATTTACCAGACCAC
AAAATCCAACCGAAAGTACTGCCGGCTTTACTCAAGCTCAAAAAATAGTAGGCAAAGCATGCGGTTTAGATGGAGTTAGG
CCAGGAATGACCTGTGAGCCAATTATGACCACGGTTGGTAGTCAAGATACTACTGGGCCAATGACTAGAGATGAACTAAA
AGAACTAGCTTGTTTAGGATTTACTGCAGATTTAGTGATGCAAAGTTTTTGTCATACAGCTGCATATCCTAAACCAGTAG
ATCTAGTTACCCATAAAGAATTACCTGATTTTATATCTCAAAGAGGTGGAGTAGCTCTTAAGCCTGGAGACGGCATAATT
CATAGTTGGCTTAACAGAATGCTGCTCCCCGATACTGTTGGCACAGGTGGAGATAGTCATACAAGATTTCCTCTTGGCAT
TTCATTTCCTGGAGGCTCGGGCATTGTTGCCTTTGCCGCTGCAATAGGATCAATGCCATTAAATATGCCGGAATCTGTAC
TGGTTAAATTTAAGGGGGAATTATTACCAGGAATTACTCTGAGAGATTTAGTAAATGCAATCCCTCTATTCGCAATTAAA
AAAGGACTCTTAACTGTTGAGAAAGAAAATAAGAAAAATATATTTAACGGTAAAATTATGGAAATTGAGGGATTACCAAA
CCTAAAACTTGAACAAGCTTTTGAACTTACTGATGCTACTGCAGAACGCTCATGCGCTGGTAGCACCATACTTTTATCCC
AAGAAACTGTTCAAGAGTACTTAAGAAGCAATATTTGCCTACTAGAAAAAATGATTGAGAGCAATTATGAAGATTCAAAA
TCGATTTCAAGAAGAATCAATGATATGAAAAATTGGTTAAAAAAACCTAAATTAATTCAGCCAGATAAGAACGCCAAGTA
TGAAGAAATCATTGAAATTGAATTAGCAAAAGTTACACAACCTATAGTTGCTTGCCCTAACGATCCAGATAATGTAAAAG
AAATCACTGATGTTGCAAATACAAATATTGACGAGGTTTTTATAGGTTCTTGCATGACAAATATTGGTCATTACAGGGCA
GCTGCGAAAGTTCTTGAAGGTGTACAAAATTTAAAATCTAAATTATGGATTTGTCCACCAACAAAAATGGATGAAGAAAC
TCTAAAAGCTGAAGGCTACTATAAAATATTCGAAGATTGTGGTGCAAGGTTAGAGTTACCAGGCTGTTCTTTATGTATGG
GAAATCAAGCCAGAGTAGATGAAGGTTCTGTAGTATTTTCTACAAGTACAAGAAATTTTGACAATAGACTTGGCAAGAAT
GCACAAGTCTTTTTAGGCAGCGCTGAATTAGCAGCAGTTTGCGCACTACTTGGAAAAATACCTGAAGTTGAAGAATATCA
GGATATTACTAAAAATAAAATTAATCCATATTCAGATGAACTTTATCGCTATCTTCAATTTGATGAAATACACGATTTCA
GCTTGACAAAGTAA

Upstream 100 bases:

>100_bases
CATTGATTAATTAAGAAGTTTGAATATTTAAAAAAGCATTTATGAATACTCTCCTTTTCAATATTAAAAATAACAATAGA
TTAGGCATTAAACCTTACTA

Downstream 100 bases:

>100_bases
TCATGGACTATGCCAAACTTTATAAAAGATAATATTCAAAAAACAAGTAATAATTCTTCTCGTAGCATTAAAAAATTATT
AAGACAAAGATCTCTAGTCG

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 857; Mature: 857

Protein sequence:

>857_residues
MKNLETLLKDYADHVAERATKGIPPLPLNAEQTNCITKLLEQDNTYDSSYLLDLLINRVPPGVDEAAYVKASWLTAIVNS
EKYCKSINPEKAIEILGTMIGGYNVNSLVEILKGENSLLAKKAAEVLKNIILVYDSANEIYELSQNNIYAKEVVNSWANA
EWFKNKKVLEKEITCLVFKVDGETNTDDLSPAVHATTRPDIPMHALAMLEFKIPDGLKILDNLKKQNLPIAYVGDVVGTG
SSRKSAINSLIWHIGEDIAYVPNKKTGGIIIGNKIAPIFFNTAQDSGALPIEADVSHMKTGDVIKIYPYKGIIKKIEQDS
NTEELISKFDLYPSTLTDEIQAGGRINLMIGRSLTDKIRTKLNYQPSEIFTRPQNPTESTAGFTQAQKIVGKACGLDGVR
PGMTCEPIMTTVGSQDTTGPMTRDELKELACLGFTADLVMQSFCHTAAYPKPVDLVTHKELPDFISQRGGVALKPGDGII
HSWLNRMLLPDTVGTGGDSHTRFPLGISFPGGSGIVAFAAAIGSMPLNMPESVLVKFKGELLPGITLRDLVNAIPLFAIK
KGLLTVEKENKKNIFNGKIMEIEGLPNLKLEQAFELTDATAERSCAGSTILLSQETVQEYLRSNICLLEKMIESNYEDSK
SISRRINDMKNWLKKPKLIQPDKNAKYEEIIEIELAKVTQPIVACPNDPDNVKEITDVANTNIDEVFIGSCMTNIGHYRA
AAKVLEGVQNLKSKLWICPPTKMDEETLKAEGYYKIFEDCGARLELPGCSLCMGNQARVDEGSVVFSTSTRNFDNRLGKN
AQVFLGSAELAAVCALLGKIPEVEEYQDITKNKINPYSDELYRYLQFDEIHDFSLTK

Sequences:

>Translated_857_residues
MKNLETLLKDYADHVAERATKGIPPLPLNAEQTNCITKLLEQDNTYDSSYLLDLLINRVPPGVDEAAYVKASWLTAIVNS
EKYCKSINPEKAIEILGTMIGGYNVNSLVEILKGENSLLAKKAAEVLKNIILVYDSANEIYELSQNNIYAKEVVNSWANA
EWFKNKKVLEKEITCLVFKVDGETNTDDLSPAVHATTRPDIPMHALAMLEFKIPDGLKILDNLKKQNLPIAYVGDVVGTG
SSRKSAINSLIWHIGEDIAYVPNKKTGGIIIGNKIAPIFFNTAQDSGALPIEADVSHMKTGDVIKIYPYKGIIKKIEQDS
NTEELISKFDLYPSTLTDEIQAGGRINLMIGRSLTDKIRTKLNYQPSEIFTRPQNPTESTAGFTQAQKIVGKACGLDGVR
PGMTCEPIMTTVGSQDTTGPMTRDELKELACLGFTADLVMQSFCHTAAYPKPVDLVTHKELPDFISQRGGVALKPGDGII
HSWLNRMLLPDTVGTGGDSHTRFPLGISFPGGSGIVAFAAAIGSMPLNMPESVLVKFKGELLPGITLRDLVNAIPLFAIK
KGLLTVEKENKKNIFNGKIMEIEGLPNLKLEQAFELTDATAERSCAGSTILLSQETVQEYLRSNICLLEKMIESNYEDSK
SISRRINDMKNWLKKPKLIQPDKNAKYEEIIEIELAKVTQPIVACPNDPDNVKEITDVANTNIDEVFIGSCMTNIGHYRA
AAKVLEGVQNLKSKLWICPPTKMDEETLKAEGYYKIFEDCGARLELPGCSLCMGNQARVDEGSVVFSTSTRNFDNRLGKN
AQVFLGSAELAAVCALLGKIPEVEEYQDITKNKINPYSDELYRYLQFDEIHDFSLTK
>Mature_857_residues
MKNLETLLKDYADHVAERATKGIPPLPLNAEQTNCITKLLEQDNTYDSSYLLDLLINRVPPGVDEAAYVKASWLTAIVNS
EKYCKSINPEKAIEILGTMIGGYNVNSLVEILKGENSLLAKKAAEVLKNIILVYDSANEIYELSQNNIYAKEVVNSWANA
EWFKNKKVLEKEITCLVFKVDGETNTDDLSPAVHATTRPDIPMHALAMLEFKIPDGLKILDNLKKQNLPIAYVGDVVGTG
SSRKSAINSLIWHIGEDIAYVPNKKTGGIIIGNKIAPIFFNTAQDSGALPIEADVSHMKTGDVIKIYPYKGIIKKIEQDS
NTEELISKFDLYPSTLTDEIQAGGRINLMIGRSLTDKIRTKLNYQPSEIFTRPQNPTESTAGFTQAQKIVGKACGLDGVR
PGMTCEPIMTTVGSQDTTGPMTRDELKELACLGFTADLVMQSFCHTAAYPKPVDLVTHKELPDFISQRGGVALKPGDGII
HSWLNRMLLPDTVGTGGDSHTRFPLGISFPGGSGIVAFAAAIGSMPLNMPESVLVKFKGELLPGITLRDLVNAIPLFAIK
KGLLTVEKENKKNIFNGKIMEIEGLPNLKLEQAFELTDATAERSCAGSTILLSQETVQEYLRSNICLLEKMIESNYEDSK
SISRRINDMKNWLKKPKLIQPDKNAKYEEIIEIELAKVTQPIVACPNDPDNVKEITDVANTNIDEVFIGSCMTNIGHYRA
AAKVLEGVQNLKSKLWICPPTKMDEETLKAEGYYKIFEDCGARLELPGCSLCMGNQARVDEGSVVFSTSTRNFDNRLGKN
AQVFLGSAELAAVCALLGKIPEVEEYQDITKNKINPYSDELYRYLQFDEIHDFSLTK

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=414, Percent_Identity=24.6376811594203, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI2367097, Length=860, Percent_Identity=58.953488372093, Blast_Score=1014, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=396, Percent_Identity=25, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6322261, Length=408, Percent_Identity=25.4901960784314, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=407, Percent_Identity=24.5700245700246, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 94594; Mature: 94594

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLETLLKDYADHVAERATKGIPPLPLNAEQTNCITKLLEQDNTYDSSYLLDLLINRVP
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
PGVDEAAYVKASWLTAIVNSEKYCKSINPEKAIEILGTMIGGYNVNSLVEILKGENSLLA
CCCCCHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHH
KKAAEVLKNIILVYDSANEIYELSQNNIYAKEVVNSWANAEWFKNKKVLEKEITCLVFKV
HHHHHHHHHHEEEECCCHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEE
DGETNTDDLSPAVHATTRPDIPMHALAMLEFKIPDGLKILDNLKKQNLPIAYVGDVVGTG
CCCCCCCCCCCCEECCCCCCCCHHHHHEEEEECCCCHHHHHHHHHCCCCEEEECCHHCCC
SSRKSAINSLIWHIGEDIAYVPNKKTGGIIIGNKIAPIFFNTAQDSGALPIEADVSHMKT
CHHHHHHHHHHHHHCCCCEECCCCCCCCEEECCCCCEEEEECCCCCCCCCEECCHHHCCC
GDVIKIYPYKGIIKKIEQDSNTEELISKFDLYPSTLTDEIQAGGRINLMIGRSLTDKIRT
CCEEEECCCHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHCCCEEEEEECCCHHHHHHH
KLNYQPSEIFTRPQNPTESTAGFTQAQKIVGKACGLDGVRPGMTCEPIMTTVGSQDTTGP
HCCCCHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCC
MTRDELKELACLGFTADLVMQSFCHTAAYPKPVDLVTHKELPDFISQRGGVALKPGDGII
CCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCHH
HSWLNRMLLPDTVGTGGDSHTRFPLGISFPGGSGIVAFAAAIGSMPLNMPESVLVKFKGE
HHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCC
LLPGITLRDLVNAIPLFAIKKGLLTVEKENKKNIFNGKIMEIEGLPNLKLEQAFELTDAT
CCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
AERSCAGSTILLSQETVQEYLRSNICLLEKMIESNYEDSKSISRRINDMKNWLKKPKLIQ
HHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
PDKNAKYEEIIEIELAKVTQPIVACPNDPDNVKEITDVANTNIDEVFIGSCMTNIGHYRA
CCCCCCHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AAKVLEGVQNLKSKLWICPPTKMDEETLKAEGYYKIFEDCGARLELPGCSLCMGNQARVD
HHHHHHHHHHHHHCEEECCCCCCCHHHHHCCCHHHHHHHCCCEEECCCCCEECCCCCCCC
EGSVVFSTSTRNFDNRLGKNAQVFLGSAELAAVCALLGKIPEVEEYQDITKNKINPYSDE
CCCEEEEECCCCHHHHHCCCCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHH
LYRYLQFDEIHDFSLTK
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKNLETLLKDYADHVAERATKGIPPLPLNAEQTNCITKLLEQDNTYDSSYLLDLLINRVP
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
PGVDEAAYVKASWLTAIVNSEKYCKSINPEKAIEILGTMIGGYNVNSLVEILKGENSLLA
CCCCCHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHH
KKAAEVLKNIILVYDSANEIYELSQNNIYAKEVVNSWANAEWFKNKKVLEKEITCLVFKV
HHHHHHHHHHEEEECCCHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEE
DGETNTDDLSPAVHATTRPDIPMHALAMLEFKIPDGLKILDNLKKQNLPIAYVGDVVGTG
CCCCCCCCCCCCEECCCCCCCCHHHHHEEEEECCCCHHHHHHHHHCCCCEEEECCHHCCC
SSRKSAINSLIWHIGEDIAYVPNKKTGGIIIGNKIAPIFFNTAQDSGALPIEADVSHMKT
CHHHHHHHHHHHHHCCCCEECCCCCCCCEEECCCCCEEEEECCCCCCCCCEECCHHHCCC
GDVIKIYPYKGIIKKIEQDSNTEELISKFDLYPSTLTDEIQAGGRINLMIGRSLTDKIRT
CCEEEECCCHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHCCCEEEEEECCCHHHHHHH
KLNYQPSEIFTRPQNPTESTAGFTQAQKIVGKACGLDGVRPGMTCEPIMTTVGSQDTTGP
HCCCCHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCC
MTRDELKELACLGFTADLVMQSFCHTAAYPKPVDLVTHKELPDFISQRGGVALKPGDGII
CCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCHH
HSWLNRMLLPDTVGTGGDSHTRFPLGISFPGGSGIVAFAAAIGSMPLNMPESVLVKFKGE
HHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCC
LLPGITLRDLVNAIPLFAIKKGLLTVEKENKKNIFNGKIMEIEGLPNLKLEQAFELTDAT
CCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
AERSCAGSTILLSQETVQEYLRSNICLLEKMIESNYEDSKSISRRINDMKNWLKKPKLIQ
HHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
PDKNAKYEEIIEIELAKVTQPIVACPNDPDNVKEITDVANTNIDEVFIGSCMTNIGHYRA
CCCCCCHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AAKVLEGVQNLKSKLWICPPTKMDEETLKAEGYYKIFEDCGARLELPGCSLCMGNQARVD
HHHHHHHHHHHHHCEEECCCCCCCHHHHHCCCHHHHHHHCCCEEECCCCCEECCCCCCCC
EGSVVFSTSTRNFDNRLGKNAQVFLGSAELAAVCALLGKIPEVEEYQDITKNKINPYSDE
CCCEEEEECCCCHHHHHCCCCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHH
LYRYLQFDEIHDFSLTK
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]