Click here to switch to the map view.

The map label for this gene is prfA

Identifier: 78780006

GI number: 78780006

Start: 1522674

End: 1523768

Strand: Reverse

Name: prfA

Synonym: PMT9312_1622

Alternate gene names: 78780006

Gene position: 1523768-1522674 (Counterclockwise)

Preceding gene: 161350028

Following gene: 78780004

Centisome position: 89.15

GC content: 36.89

Gene sequence:

>1095_bases
TTGGAATACTCAACACTAATTGCAAGGTTGAAAACTGCTTCAGAAAGTTTTGAAAATTTGGAAGTGCAGCTTGCAGATCC
TGATATAGCTAATGATCCTAAAAAATTAGAATCAATAGCAAGAGAAAGGTCAAAGTTGGAACCTTTGGTGATTGATTTTA
ATAAGTTGCTTGATACCGATAAAGAAATTGAAGATTCAAAAAATCTACTGAAAGAGAATCGAAATGATAAAGAGATGGAA
TCTTTAATAAATGAAGAGTTAATTAACCTTGAGGAATGTAAGAATCAACTTATTCAAAAAACTACAATCGCCTTATTACC
TAAAGATCCAAGAGATGAGAGAAGTGTAATGTTAGAGATCAGAGCTGGTGCTGGAGGTAGCGAAGCTTGTATATGGGCGG
GTGATTTAGCAAGAATGTATGAGAGATACGGACAAAAAATCGGATGGTCTGTAAAACCTGTTAGTGCTTCCGAGTCAGAT
ATGGGAGGATTTAAGGAGCTAGTTATCTCAGTTAAAGGAGATTCTGTATATAGTCAACTTAAATTCGAGGCTGGCGTACA
TAGAGTTCAAAGAGTTCCAGCTACTGAGTCTCAAGGTAGAGTTCATACCTCTACAGCTACAGTTGCTGTCATGCCTGAAG
CAGATCCTGTTGAGGTTAAGATTGATCCTACCGATTTAGAGGTTGGCACAGCAAGATCAGGAGGTGCAGGGGGCCAAAAT
GTGAATAAGGTTGAGACTGCTATTGATCTTCTCCATAAGCCCACAGGAATAAGAGTTTTTTGTACTCAAGAGAGATCACA
ATTGCAAAATCGTGAGCGAGCAATGGAAATTTTAAGAGCTAAATTGTATGAAATTCAATTAAAAGAAGCTAATGCAAAAG
AGAGATCACAAAGGCTTTCACAGGTTGGAACTGGTGATAGGAGTGAAAAAATTAGAACTTATAATTTTAAAGATAACAGA
ACAACTGATCATAGATTAGGTTCCAATTTTTCGCTAGAACCAATTCTCGCTGGTCAGTTGGATGAAGTGATTGATGCATG
TATAGCGCAAGAGCAAAAGAGAATGATGGAAGATTTTAATAACGATGTGAATTAA

Upstream 100 bases:

>100_bases
AAATATGGAATGGGTTCAGCTAATTCAGCTACATCAAAAGAGCAAAAAGAAGAAAAAGATTCTAATAAATAGAATTTTCA
AAAATTAAGCATAATTTCAA

Downstream 100 bases:

>100_bases
GATTTTATTTTCTTAGATTGCAACCCCTATTACATATTTACTCCATTCTTTATGTCTGCCAGAGTCAATTTGTCTTGTAG
CCTCAAAATTGAGATTACTT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MEYSTLIARLKTASESFENLEVQLADPDIANDPKKLESIARERSKLEPLVIDFNKLLDTDKEIEDSKNLLKENRNDKEME
SLINEELINLEECKNQLIQKTTIALLPKDPRDERSVMLEIRAGAGGSEACIWAGDLARMYERYGQKIGWSVKPVSASESD
MGGFKELVISVKGDSVYSQLKFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVEVKIDPTDLEVGTARSGGAGGQN
VNKVETAIDLLHKPTGIRVFCTQERSQLQNRERAMEILRAKLYEIQLKEANAKERSQRLSQVGTGDRSEKIRTYNFKDNR
TTDHRLGSNFSLEPILAGQLDEVIDACIAQEQKRMMEDFNNDVN

Sequences:

>Translated_364_residues
MEYSTLIARLKTASESFENLEVQLADPDIANDPKKLESIARERSKLEPLVIDFNKLLDTDKEIEDSKNLLKENRNDKEME
SLINEELINLEECKNQLIQKTTIALLPKDPRDERSVMLEIRAGAGGSEACIWAGDLARMYERYGQKIGWSVKPVSASESD
MGGFKELVISVKGDSVYSQLKFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVEVKIDPTDLEVGTARSGGAGGQN
VNKVETAIDLLHKPTGIRVFCTQERSQLQNRERAMEILRAKLYEIQLKEANAKERSQRLSQVGTGDRSEKIRTYNFKDNR
TTDHRLGSNFSLEPILAGQLDEVIDACIAQEQKRMMEDFNNDVN
>Mature_364_residues
MEYSTLIARLKTASESFENLEVQLADPDIANDPKKLESIARERSKLEPLVIDFNKLLDTDKEIEDSKNLLKENRNDKEME
SLINEELINLEECKNQLIQKTTIALLPKDPRDERSVMLEIRAGAGGSEACIWAGDLARMYERYGQKIGWSVKPVSASESD
MGGFKELVISVKGDSVYSQLKFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVEVKIDPTDLEVGTARSGGAGGQN
VNKVETAIDLLHKPTGIRVFCTQERSQLQNRERAMEILRAKLYEIQLKEANAKERSQRLSQVGTGDRSEKIRTYNFKDNR
TTDHRLGSNFSLEPILAGQLDEVIDACIAQEQKRMMEDFNNDVN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=304, Percent_Identity=40.4605263157895, Blast_Score=238, Evalue=9e-63,
Organism=Homo sapiens, GI34577120, Length=353, Percent_Identity=34.8441926345609, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI166795305, Length=209, Percent_Identity=36.8421052631579, Blast_Score=155, Evalue=4e-38,
Organism=Escherichia coli, GI1787462, Length=350, Percent_Identity=42.8571428571429, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=34.3195266272189, Blast_Score=201, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI17542784, Length=307, Percent_Identity=36.1563517915309, Blast_Score=184, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=267, Percent_Identity=42.6966292134831, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI19921226, Length=311, Percent_Identity=38.2636655948553, Blast_Score=209, Evalue=2e-54,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40854; Mature: 40854

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYSTLIARLKTASESFENLEVQLADPDIANDPKKLESIARERSKLEPLVIDFNKLLDTD
CCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHCCC
KEIEDSKNLLKENRNDKEMESLINEELINLEECKNQLIQKTTIALLPKDPRDERSVMLEI
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHEECCCCCCCCCEEEEEE
RAGAGGSEACIWAGDLARMYERYGQKIGWSVKPVSASESDMGGFKELVISVKGDSVYSQL
ECCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHEEEECCCHHHHHH
KFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVEVKIDPTDLEVGTARSGGAGGQN
HHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCEEEEECCCCCEECCCCCCCCCCCC
VNKVETAIDLLHKPTGIRVFCTQERSQLQNRERAMEILRAKLYEIQLKEANAKERSQRLS
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QVGTGDRSEKIRTYNFKDNRTTDHRLGSNFSLEPILAGQLDEVIDACIAQEQKRMMEDFN
HHCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
NDVN
CCCC
>Mature Secondary Structure
MEYSTLIARLKTASESFENLEVQLADPDIANDPKKLESIARERSKLEPLVIDFNKLLDTD
CCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHCCC
KEIEDSKNLLKENRNDKEMESLINEELINLEECKNQLIQKTTIALLPKDPRDERSVMLEI
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHEECCCCCCCCCEEEEEE
RAGAGGSEACIWAGDLARMYERYGQKIGWSVKPVSASESDMGGFKELVISVKGDSVYSQL
ECCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHEEEECCCHHHHHH
KFEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVEVKIDPTDLEVGTARSGGAGGQN
HHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCEEEEECCCCCEECCCCCCCCCCCC
VNKVETAIDLLHKPTGIRVFCTQERSQLQNRERAMEILRAKLYEIQLKEANAKERSQRLS
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QVGTGDRSEKIRTYNFKDNRTTDHRLGSNFSLEPILAGQLDEVIDACIAQEQKRMMEDFN
HHCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
NDVN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA