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The map label for this gene is rne [H]

Identifier: 78779977

GI number: 78779977

Start: 1491074

End: 1492891

Strand: Direct

Name: rne [H]

Synonym: PMT9312_1593

Alternate gene names: 78779977

Gene position: 1491074-1492891 (Clockwise)

Preceding gene: 78779974

Following gene: 78779978

Centisome position: 87.24

GC content: 29.92

Gene sequence:

>1818_bases
ATGTCTCAGCAAATTATCATCGCTGAGCAGGCTCGAATTGCAGCACTACTCAAAGATGATCGAGTTGATGAATTAATCGT
CGCACAAGGTCAATATCAAATTGGCGATATTTTTTTAGGAACAGTTGAAAATGTTCTTCCTGGTATTGATGCCGCTTTCA
TAAATATTGGTGAAAGTGATAAAAATGGATTCATCCATGTTTCAGATCTAGGTCCATTGAGACTCAAAAAAGGGACATTT
GGAATAACTGAATTACTAGAACCAAAACAAAAAGTTCTAGTACAAGTAATAAAGGAACCCACAGGATCTAAAGGGCCTAG
ATTAACCGGGAATATTTCAATCCCTGGGAAATACATAATATTACAGCCATATGGCCAAGGGGTAAATATTTCAAGAAAAA
TAAATACAGAAACAGAACGAAGCCGTTTAAAAGCTCTTGGAGTTTTAATAAAACCACCTAGCACAGGTTTAGTATTCAGA
ACAGAGGCTGAAAAGATAAAAGAAGAACTACTAATTGATGATTTAGAAAAATTAATTCAACAATGGGAAAACATATTGAA
AATTTCTGAGGCGTCTAATCCACCAAATTTAGTAAAAAGAGATGATGATTTCTCTCTCAAAATCTTAAGAGATCATATTA
AATCATCAACTAAAAGCATAATTATCGATAGTAAATTTTCAGTTGAAAGGGCAAAAGATTTTTTAATAAATTATGAATCT
AATATAGGTATTGAATTTCACGATAACAATTTAAACCAAAATATTTTAGAGAAATATGAAATCAAGAAAACAATTCAAAA
AGCTCTTCAGCCAAGAGTAGATCTACCGTCAGGGGGTTATATAATTATTGAACCTACTGAAGCCTTAACAGTAATTGACG
TAAACTCTGGATCATTTACAAGATCCGCTAACTCAAGACAAACCGTTTTGTGGACGAATTGCGAAGCAGCAGTTGAAATC
TCAAGACAAATGAAATTAAGAAATATAGGTGGAGTTATTGTTGTAGATTTTATTGATATGGAATCTAGAAGAGATCAATT
CCAATTACTTGAGCATTTTACTTCAGCGATAAAAGATGATTCTGCTAGACCTCAAATAGCTCAGCTTACTGAATTAGGTT
TAGTTGAGTTAACGAGAAAAAGACAGGGTCAAAATATATATGAATTATTCGGTAAAAAATGTTCCATATGCAATGGAACA
GGACATGTAGAAAATATATTGAATAATGAAAATCCTAACCTAAAAATTAAAAATATTGACGAAAAATCTAATCAATCAAA
CATTATAAAACCGTTGGATATAGATACTTCTAAATCAGCTGAAAATCAGGAGAAAATAATTGAAAAGGAATTACTTAACC
AACAGAACTTAAGTAAGGAGAATTCTTCTAATAAAAAAGAAAATGATAATGATGATTTGAACAGATCAAATTCAAAAGAA
AAAAACATAATAACTGTTGATCTTACTAATGAAGAAAAAATTGTTTTCAGCCAATTAGGTATTAATCCACTAATCAAGTT
AGGTAAAGAATACCTAACCAGTAATAATTTTGTGCGTTTAAAAGAGAATAGTAAGGAACAAGATAAAACTTTAGATAATA
AAAAAACAAAATCAAAACAAATTAAAAAAATCTCAAACTCAAAAGAAGGAATGATTGAGATTAATATTGAAACAAATGCA
AATCCCAAAGATAAATCAAAAAAAACTAATAAAAAAAATGAAGTTATATTTATAGATAAGAAGGATGAAGTTGAACTTAC
CGATGAAATAAGCAATGCGAGAAAAAAAAGAAGAAGATCTTCAGCAAGTATTGAATAA

Upstream 100 bases:

>100_bases
AGTAAATAATACTAGCTATAAAAATTGCTTTCTTAAAAGATAAAATCTAATTCTTTTCTTTAGATGATTTTCAAAATTCA
AACCATTCAGAATCTACTAT

Downstream 100 bases:

>100_bases
AGTATCCGAAGTTGGAATAGATGAAGTTGGAAGGGGAGCAGTTTTTGGTCCAGTTTTCTCCGCTGTTGTAGTTTTAACTG
AAAAAAATAAATTTATCTTG

Product: ribonuclease

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 605; Mature: 604

Protein sequence:

>605_residues
MSQQIIIAEQARIAALLKDDRVDELIVAQGQYQIGDIFLGTVENVLPGIDAAFINIGESDKNGFIHVSDLGPLRLKKGTF
GITELLEPKQKVLVQVIKEPTGSKGPRLTGNISIPGKYIILQPYGQGVNISRKINTETERSRLKALGVLIKPPSTGLVFR
TEAEKIKEELLIDDLEKLIQQWENILKISEASNPPNLVKRDDDFSLKILRDHIKSSTKSIIIDSKFSVERAKDFLINYES
NIGIEFHDNNLNQNILEKYEIKKTIQKALQPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRSANSRQTVLWTNCEAAVEI
SRQMKLRNIGGVIVVDFIDMESRRDQFQLLEHFTSAIKDDSARPQIAQLTELGLVELTRKRQGQNIYELFGKKCSICNGT
GHVENILNNENPNLKIKNIDEKSNQSNIIKPLDIDTSKSAENQEKIIEKELLNQQNLSKENSSNKKENDNDDLNRSNSKE
KNIITVDLTNEEKIVFSQLGINPLIKLGKEYLTSNNFVRLKENSKEQDKTLDNKKTKSKQIKKISNSKEGMIEINIETNA
NPKDKSKKTNKKNEVIFIDKKDEVELTDEISNARKKRRRSSASIE

Sequences:

>Translated_605_residues
MSQQIIIAEQARIAALLKDDRVDELIVAQGQYQIGDIFLGTVENVLPGIDAAFINIGESDKNGFIHVSDLGPLRLKKGTF
GITELLEPKQKVLVQVIKEPTGSKGPRLTGNISIPGKYIILQPYGQGVNISRKINTETERSRLKALGVLIKPPSTGLVFR
TEAEKIKEELLIDDLEKLIQQWENILKISEASNPPNLVKRDDDFSLKILRDHIKSSTKSIIIDSKFSVERAKDFLINYES
NIGIEFHDNNLNQNILEKYEIKKTIQKALQPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRSANSRQTVLWTNCEAAVEI
SRQMKLRNIGGVIVVDFIDMESRRDQFQLLEHFTSAIKDDSARPQIAQLTELGLVELTRKRQGQNIYELFGKKCSICNGT
GHVENILNNENPNLKIKNIDEKSNQSNIIKPLDIDTSKSAENQEKIIEKELLNQQNLSKENSSNKKENDNDDLNRSNSKE
KNIITVDLTNEEKIVFSQLGINPLIKLGKEYLTSNNFVRLKENSKEQDKTLDNKKTKSKQIKKISNSKEGMIEINIETNA
NPKDKSKKTNKKNEVIFIDKKDEVELTDEISNARKKRRRSSASIE
>Mature_604_residues
SQQIIIAEQARIAALLKDDRVDELIVAQGQYQIGDIFLGTVENVLPGIDAAFINIGESDKNGFIHVSDLGPLRLKKGTFG
ITELLEPKQKVLVQVIKEPTGSKGPRLTGNISIPGKYIILQPYGQGVNISRKINTETERSRLKALGVLIKPPSTGLVFRT
EAEKIKEELLIDDLEKLIQQWENILKISEASNPPNLVKRDDDFSLKILRDHIKSSTKSIIIDSKFSVERAKDFLINYESN
IGIEFHDNNLNQNILEKYEIKKTIQKALQPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRSANSRQTVLWTNCEAAVEIS
RQMKLRNIGGVIVVDFIDMESRRDQFQLLEHFTSAIKDDSARPQIAQLTELGLVELTRKRQGQNIYELFGKKCSICNGTG
HVENILNNENPNLKIKNIDEKSNQSNIIKPLDIDTSKSAENQEKIIEKELLNQQNLSKENSSNKKENDNDDLNRSNSKEK
NIITVDLTNEEKIVFSQLGINPLIKLGKEYLTSNNFVRLKENSKEQDKTLDNKKTKSKQIKKISNSKEGMIEINIETNAN
PKDKSKKTNKKNEVIFIDKKDEVELTDEISNARKKRRRSSASIE

Specific function: Involved In The Processing Of The 5'End Of 16s Rrna. Could Be Involved In Chromosome Segregation And Cell Division. It May Be One Of The Components Of The Cytoplasmic Axial Filaments Bundles Or Merely Regulate The Formation Of This Structure. [C]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=381, Percent_Identity=33.8582677165354, Blast_Score=218, Evalue=1e-57,
Organism=Escherichia coli, GI1787325, Length=418, Percent_Identity=33.0143540669856, Blast_Score=209, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 68353; Mature: 68222

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQIIIAEQARIAALLKDDRVDELIVAQGQYQIGDIFLGTVENVLPGIDAAFINIGESD
CCCCEEEECHHHHHHHHHCCCCCCEEEECCCEEECCEEHHHHHHHHCCCCEEEEECCCCC
KNGFIHVSDLGPLRLKKGTFGITELLEPKQKVLVQVIKEPTGSKGPRLTGNISIPGKYII
CCCEEEEECCCCEEEECCCCCHHHHHCHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEE
LQPYGQGVNISRKINTETERSRLKALGVLIKPPSTGLVFRTEAEKIKEELLIDDLEKLIQ
EEECCCCCEEEEECCCHHHHHHHHHHEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHHH
QWENILKISEASNPPNLVKRDDDFSLKILRDHIKSSTKSIIIDSKFSVERAKDFLINYES
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHEEEECC
NIGIEFHDNNLNQNILEKYEIKKTIQKALQPRVDLPSGGYIIIEPTEALTVIDVNSGSFT
CCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEECCCCEE
RSANSRQTVLWTNCEAAVEISRQMKLRNIGGVIVVDFIDMESRRDQFQLLEHFTSAIKDD
ECCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCC
SARPQIAQLTELGLVELTRKRQGQNIYELFGKKCSICNGTGHVENILNNENPNLKIKNID
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCCCEEEEECC
EKSNQSNIIKPLDIDTSKSAENQEKIIEKELLNQQNLSKENSSNKKENDNDDLNRSNSKE
CCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCHHCCCCCCCCCCCCCCCCCCCCCCCCC
KNIITVDLTNEEKIVFSQLGINPLIKLGKEYLTSNNFVRLKENSKEQDKTLDNKKTKSKQ
CCEEEEEECCCCEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHH
IKKISNSKEGMIEINIETNANPKDKSKKTNKKNEVIFIDKKDEVELTDEISNARKKRRRS
HHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
SASIE
CCCCC
>Mature Secondary Structure 
SQQIIIAEQARIAALLKDDRVDELIVAQGQYQIGDIFLGTVENVLPGIDAAFINIGESD
CCCEEEECHHHHHHHHHCCCCCCEEEECCCEEECCEEHHHHHHHHCCCCEEEEECCCCC
KNGFIHVSDLGPLRLKKGTFGITELLEPKQKVLVQVIKEPTGSKGPRLTGNISIPGKYII
CCCEEEEECCCCEEEECCCCCHHHHHCHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEE
LQPYGQGVNISRKINTETERSRLKALGVLIKPPSTGLVFRTEAEKIKEELLIDDLEKLIQ
EEECCCCCEEEEECCCHHHHHHHHHHEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHHH
QWENILKISEASNPPNLVKRDDDFSLKILRDHIKSSTKSIIIDSKFSVERAKDFLINYES
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHEEEECC
NIGIEFHDNNLNQNILEKYEIKKTIQKALQPRVDLPSGGYIIIEPTEALTVIDVNSGSFT
CCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEECCCCEE
RSANSRQTVLWTNCEAAVEISRQMKLRNIGGVIVVDFIDMESRRDQFQLLEHFTSAIKDD
ECCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCC
SARPQIAQLTELGLVELTRKRQGQNIYELFGKKCSICNGTGHVENILNNENPNLKIKNID
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCCCEEEEECC
EKSNQSNIIKPLDIDTSKSAENQEKIIEKELLNQQNLSKENSSNKKENDNDDLNRSNSKE
CCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCHHCCCCCCCCCCCCCCCCCCCCCCCCC
KNIITVDLTNEEKIVFSQLGINPLIKLGKEYLTSNNFVRLKENSKEQDKTLDNKKTKSKQ
CCEEEEEECCCCEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHH
IKKISNSKEGMIEINIETNANPKDKSKKTNKKNEVIFIDKKDEVELTDEISNARKKRRRS
HHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
SASIE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]