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The map label for this gene is nusA

Identifier: 78779969

GI number: 78779969

Start: 1480736

End: 1482142

Strand: Direct

Name: nusA

Synonym: PMT9312_1585

Alternate gene names: 78779969

Gene position: 1480736-1482142 (Clockwise)

Preceding gene: 78779968

Following gene: 78779970

Centisome position: 86.63

GC content: 37.88

Gene sequence:

>1407_bases
ATGGCATTAGTTATTCTACCAGGTTTAAACAATCTCATTGAGGACATTAGTGAGGAAAAAAAGTTACCTCCTAATATTGT
GGAACTAGCCTTGCGCGAAGCTTTATTAAAAGGATACGAAAAATATAGAAAAACTTTTTACATTGGAGTTGTTGAAGATC
CATTTGATGAAGAATATTTTAATAATTTTGATGTTGGACTAGATCTAGATGAAGAAGGTTATAGGGTATTATCGAGTAAA
ATAATTGTTGAAGAGGTTGAGAGCGAAGATCATCAAATATCTCTAGCTGAAGTTAAACAGGTAGCTGAAGATGCTCAAAT
AGGTGACACAGTTGTTTTAGACGTTACTCCAGAAAAAGAGGATTTTGGACGAATGGCTGCTTCAACAACAAAGCAAGTGT
TAGCCCAAAAATTAAGAGATCAACAAAGAAAAATGATCCAGGAAGAATTTGCGGATTTGGAAGACCCTGTTTTAACTGCA
AGAGTTATAAGATTTGAAAGGCAATCAGTCATTATGGGAGTAAGTTCTGGTATTGGTAGACCTGAAGTTGAGGCCGAACT
TCCCAAGAGAGATCAATTACCTAATGATAATTACAGAGCAAATGCAACTTTTAAAGTATTTTTGAAAGAAGTTAGCGAAA
TAGCCAGAAAAGGACCACAACTTTTTGTCAGTAGAGCAAATGCTGGTTTAGTGGTTTATTTATTTGAAAATGAAGTCCCG
GAAATTCAAGAAGGTACAGTAAAAATTGTTGCTGTTTCAAGAGAAGCAAACCCTCCTTCAAGAGGTGTTGGCCCAAGAAC
GAAAGTAGCAGTAGACAGTGTAGAACAAGAAGTGGACCCCGTAGGAGCGTGTATTGGGGCTAGAGGAGCGAGAATTCAAC
AAGTAGTTAATGAACTAAGAGGAGAAAAAATTGATGTTATAAAATGGTCATCTGATCCAATACAGTATATTTTAAACTCT
TTAAGTCCTGCGAAAGTTGATCAAGTAAGACTAGTTGACCCAGCAGGGCAACATGCCCATGTACTAGTTCCTCCTGATCA
ATTGAGTCTCGCAATTGGTAGAGAAGGCCAAAATGTAAGACTTGCCGCAAGATTAACTGGTTGGAAGATTGATGTTAAAA
ACTCACATGAATACGATCAGAAAGCTGAAGATGCGGCAGTATCTGAATTAATCATTCAAAGGGAAGATGAAGAGAATCTC
CAGCGAGAAGCTGAGCTAAGATTAGAAGCAGAACAAGCTGAGCGCGCTGCAGAAGATGCAAGATTAAGAGAGCTTTATCC
CCTTCCAGAAGATGATCAAGAATATGAAGAAGAAGAACAATACGGAGAAGGAGACTTATCAGATAATGACCAATTAGAGA
CTCTTCAAGATAGTGAAATTGCCGCAAAAGAGGAGAGAAAGCGGTGA

Upstream 100 bases:

>100_bases
GTAAGATTAAAAAAATCCCTTTTAATGAAGTACTCAAGATTAGTCTTTGCACATTAAAAGATTAATTAATTTTCAACCAT
TATTCAATTTCCCATCATAG

Downstream 100 bases:

>100_bases
TACAACAAAGACCTGTTTTGCGTATATGCATTTCATGTAGAAAAACATATGACAGGAAAAATCTTTTAAAGATTACCAAA
GATCATAAGCAAGGCATTAT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MALVILPGLNNLIEDISEEKKLPPNIVELALREALLKGYEKYRKTFYIGVVEDPFDEEYFNNFDVGLDLDEEGYRVLSSK
IIVEEVESEDHQISLAEVKQVAEDAQIGDTVVLDVTPEKEDFGRMAASTTKQVLAQKLRDQQRKMIQEEFADLEDPVLTA
RVIRFERQSVIMGVSSGIGRPEVEAELPKRDQLPNDNYRANATFKVFLKEVSEIARKGPQLFVSRANAGLVVYLFENEVP
EIQEGTVKIVAVSREANPPSRGVGPRTKVAVDSVEQEVDPVGACIGARGARIQQVVNELRGEKIDVIKWSSDPIQYILNS
LSPAKVDQVRLVDPAGQHAHVLVPPDQLSLAIGREGQNVRLAARLTGWKIDVKNSHEYDQKAEDAAVSELIIQREDEENL
QREAELRLEAEQAERAAEDARLRELYPLPEDDQEYEEEEQYGEGDLSDNDQLETLQDSEIAAKEERKR

Sequences:

>Translated_468_residues
MALVILPGLNNLIEDISEEKKLPPNIVELALREALLKGYEKYRKTFYIGVVEDPFDEEYFNNFDVGLDLDEEGYRVLSSK
IIVEEVESEDHQISLAEVKQVAEDAQIGDTVVLDVTPEKEDFGRMAASTTKQVLAQKLRDQQRKMIQEEFADLEDPVLTA
RVIRFERQSVIMGVSSGIGRPEVEAELPKRDQLPNDNYRANATFKVFLKEVSEIARKGPQLFVSRANAGLVVYLFENEVP
EIQEGTVKIVAVSREANPPSRGVGPRTKVAVDSVEQEVDPVGACIGARGARIQQVVNELRGEKIDVIKWSSDPIQYILNS
LSPAKVDQVRLVDPAGQHAHVLVPPDQLSLAIGREGQNVRLAARLTGWKIDVKNSHEYDQKAEDAAVSELIIQREDEENL
QREAELRLEAEQAERAAEDARLRELYPLPEDDQEYEEEEQYGEGDLSDNDQLETLQDSEIAAKEERKR
>Mature_467_residues
ALVILPGLNNLIEDISEEKKLPPNIVELALREALLKGYEKYRKTFYIGVVEDPFDEEYFNNFDVGLDLDEEGYRVLSSKI
IVEEVESEDHQISLAEVKQVAEDAQIGDTVVLDVTPEKEDFGRMAASTTKQVLAQKLRDQQRKMIQEEFADLEDPVLTAR
VIRFERQSVIMGVSSGIGRPEVEAELPKRDQLPNDNYRANATFKVFLKEVSEIARKGPQLFVSRANAGLVVYLFENEVPE
IQEGTVKIVAVSREANPPSRGVGPRTKVAVDSVEQEVDPVGACIGARGARIQQVVNELRGEKIDVIKWSSDPIQYILNSL
SPAKVDQVRLVDPAGQHAHVLVPPDQLSLAIGREGQNVRLAARLTGWKIDVKNSHEYDQKAEDAAVSELIIQREDEENLQ
REAELRLEAEQAERAAEDARLRELYPLPEDDQEYEEEEQYGEGDLSDNDQLETLQDSEIAAKEERKR

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=372, Percent_Identity=32.258064516129, Blast_Score=187, Evalue=1e-48,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 52696; Mature: 52564

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVILPGLNNLIEDISEEKKLPPNIVELALREALLKGYEKYRKTFYIGVVEDPFDEEYF
CEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHH
NNFDVGLDLDEEGYRVLSSKIIVEEVESEDHQISLAEVKQVAEDAQIGDTVVLDVTPEKE
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCHH
DFGRMAASTTKQVLAQKLRDQQRKMIQEEFADLEDPVLTARVIRFERQSVIMGVSSGIGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEHHCCCCC
PEVEAELPKRDQLPNDNYRANATFKVFLKEVSEIARKGPQLFVSRANAGLVVYLFENEVP
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCC
EIQEGTVKIVAVSREANPPSRGVGPRTKVAVDSVEQEVDPVGACIGARGARIQQVVNELR
CCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GEKIDVIKWSSDPIQYILNSLSPAKVDQVRLVDPAGQHAHVLVPPDQLSLAIGREGQNVR
CCEEEEEEECCCHHHHHHHCCCCCCCCEEEEECCCCCCCEEEECCHHEEEEECCCCCCEE
LAARLTGWKIDVKNSHEYDQKAEDAAVSELIIQREDEENLQREAELRLEAEQAERAAEDA
EEEEEECEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHH
RLRELYPLPEDDQEYEEEEQYGEGDLSDNDQLETLQDSEIAAKEERKR
HHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
ALVILPGLNNLIEDISEEKKLPPNIVELALREALLKGYEKYRKTFYIGVVEDPFDEEYF
EEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHH
NNFDVGLDLDEEGYRVLSSKIIVEEVESEDHQISLAEVKQVAEDAQIGDTVVLDVTPEKE
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCHH
DFGRMAASTTKQVLAQKLRDQQRKMIQEEFADLEDPVLTARVIRFERQSVIMGVSSGIGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEHHCCCCC
PEVEAELPKRDQLPNDNYRANATFKVFLKEVSEIARKGPQLFVSRANAGLVVYLFENEVP
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCC
EIQEGTVKIVAVSREANPPSRGVGPRTKVAVDSVEQEVDPVGACIGARGARIQQVVNELR
CCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GEKIDVIKWSSDPIQYILNSLSPAKVDQVRLVDPAGQHAHVLVPPDQLSLAIGREGQNVR
CCEEEEEEECCCHHHHHHHCCCCCCCCEEEEECCCCCCCEEEECCHHEEEEECCCCCCEE
LAARLTGWKIDVKNSHEYDQKAEDAAVSELIIQREDEENLQREAELRLEAEQAERAAEDA
EEEEEECEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHH
RLRELYPLPEDDQEYEEEEQYGEGDLSDNDQLETLQDSEIAAKEERKR
HHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]