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The map label for this gene is rlmN [H]

Identifier: 78779958

GI number: 78779958

Start: 1464783

End: 1465829

Strand: Reverse

Name: rlmN [H]

Synonym: PMT9312_1574

Alternate gene names: 78779958

Gene position: 1465829-1464783 (Counterclockwise)

Preceding gene: 78779959

Following gene: 78779957

Centisome position: 85.76

GC content: 31.81

Gene sequence:

>1047_bases
TTGAAAAATCTTCTTGGAAGTAGCATTAAAGATTTAGAAAATATAGCTTTAGATTATGGCCAGGCTGCTTTTAGAGGTCG
CCAAATCTACAGTTGGATATATAACTATAGAAATAAGAATAAAAATATTGATCAAATAGAAGTTTTACCTTTAGATTTCA
GAAAAAAGTTAAAGGATGATGGTTTTAAAGTAAGTGAACTTAGTTTTCAAGAAAAAAAGTTGGCCAATGATGGCACTTTA
AAGTTGTTACTGTCTACAAATGATAATGAAAGCATTGAGTGTGTCGGCATACCCACTGAAAAAAGGCTAACTGCATGTCT
GTCGAGTCAAGTTGGATGTCCTATGGACTGCAAATTTTGCGCAACGGGGAAAGAGGGTTTGAAGAGATCTTTAAAAGCAA
GTGAAATCTTAGATCAAATTTTATTTATTGAAAATGAAATGAATAGAAAAGTGACTAATATTGTTTTCATGGGAATGGGC
GAGCCATTGTTGAATATTGATGAATTGCTTTTGTCAATAAGATCAATAAATGAGGATTTTCAGATTAGTCAGAGAAAGAT
AACTGTAAGCACAGTTGCTGTTCCAAAAATGATGAGTAAATTATCAGCAAGGTCTTTTCAAATTTTAGGTAATTGTCAAT
TTACATTAGCAATTAGCCTACATGCTTCAAATCAGAAAACAAGGGAAACCATAATACCAAGCGCAAAAAACTACGAGATC
AAAAATATAATTGAAGACTCTAAGCAATTTGTAAAAGATACTGGTCGGAGGGTAAGTTTTGAATATTTAATGCTAAGTGG
GGTTAATGACAAATTAGAACATGCCAACGAATTGAGTAATTTGCTAAGAGGTTTTCAATGCCACGTCAATTTAATACAGT
ACAACCAAATTGATGAGGTTGAATTTAAACGAGCATCTTTAAAAAATCTTCAATTATTTCAATCTAGACTTTCTAATAAT
GGTATCACTGTTAGCTTTCGAAAAAGTAGAGGTTTAGATAAAAATGCAGCATGTGGTCAGTTAAGACAAAATGCAAGAAA
TAAATAA

Upstream 100 bases:

>100_bases
CGGCCATGATTGGATTTATTGCGCTTATTCTTACAGAATTCTTTTTAAAACATGGTTTGCTGTTATGGTGAAAATAGTTT
TTAACTAAAGAATTAATAAT

Downstream 100 bases:

>100_bases
TATTATCTAAGAATAATTCTTTAAAAGTATATTAAACAGGTTATTATTGACTTAAATAATTTTGAATCTTTGGGTTTTAT
TAAGACAAAAATTTTACCTT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKNLLGSSIKDLENIALDYGQAAFRGRQIYSWIYNYRNKNKNIDQIEVLPLDFRKKLKDDGFKVSELSFQEKKLANDGTL
KLLLSTNDNESIECVGIPTEKRLTACLSSQVGCPMDCKFCATGKEGLKRSLKASEILDQILFIENEMNRKVTNIVFMGMG
EPLLNIDELLLSIRSINEDFQISQRKITVSTVAVPKMMSKLSARSFQILGNCQFTLAISLHASNQKTRETIIPSAKNYEI
KNIIEDSKQFVKDTGRRVSFEYLMLSGVNDKLEHANELSNLLRGFQCHVNLIQYNQIDEVEFKRASLKNLQLFQSRLSNN
GITVSFRKSRGLDKNAACGQLRQNARNK

Sequences:

>Translated_348_residues
MKNLLGSSIKDLENIALDYGQAAFRGRQIYSWIYNYRNKNKNIDQIEVLPLDFRKKLKDDGFKVSELSFQEKKLANDGTL
KLLLSTNDNESIECVGIPTEKRLTACLSSQVGCPMDCKFCATGKEGLKRSLKASEILDQILFIENEMNRKVTNIVFMGMG
EPLLNIDELLLSIRSINEDFQISQRKITVSTVAVPKMMSKLSARSFQILGNCQFTLAISLHASNQKTRETIIPSAKNYEI
KNIIEDSKQFVKDTGRRVSFEYLMLSGVNDKLEHANELSNLLRGFQCHVNLIQYNQIDEVEFKRASLKNLQLFQSRLSNN
GITVSFRKSRGLDKNAACGQLRQNARNK
>Mature_348_residues
MKNLLGSSIKDLENIALDYGQAAFRGRQIYSWIYNYRNKNKNIDQIEVLPLDFRKKLKDDGFKVSELSFQEKKLANDGTL
KLLLSTNDNESIECVGIPTEKRLTACLSSQVGCPMDCKFCATGKEGLKRSLKASEILDQILFIENEMNRKVTNIVFMGMG
EPLLNIDELLLSIRSINEDFQISQRKITVSTVAVPKMMSKLSARSFQILGNCQFTLAISLHASNQKTRETIIPSAKNYEI
KNIIEDSKQFVKDTGRRVSFEYLMLSGVNDKLEHANELSNLLRGFQCHVNLIQYNQIDEVEFKRASLKNLQLFQSRLSNN
GITVSFRKSRGLDKNAACGQLRQNARNK

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=351, Percent_Identity=34.7578347578348, Blast_Score=213, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 39552; Mature: 39552

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLLGSSIKDLENIALDYGQAAFRGRQIYSWIYNYRNKNKNIDQIEVLPLDFRKKLKDD
CCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHCCC
GFKVSELSFQEKKLANDGTLKLLLSTNDNESIECVGIPTEKRLTACLSSQVGCPMDCKFC
CCEEHHCCHHHHHCCCCCEEEEEEECCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEE
ATGKEGLKRSLKASEILDQILFIENEMNRKVTNIVFMGMGEPLLNIDELLLSIRSINEDF
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCC
QISQRKITVSTVAVPKMMSKLSARSFQILGNCQFTLAISLHASNQKTRETIIPSAKNYEI
EECCEEEEEHHHHHHHHHHHHHHCCEEEEECEEEEEEEEEECCCCHHHHHHCCCCCCCHH
KNIIEDSKQFVKDTGRRVSFEYLMLSGVNDKLEHANELSNLLRGFQCHVNLIQYNQIDEV
HHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCHH
EFKRASLKNLQLFQSRLSNNGITVSFRKSRGLDKNAACGQLRQNARNK
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MKNLLGSSIKDLENIALDYGQAAFRGRQIYSWIYNYRNKNKNIDQIEVLPLDFRKKLKDD
CCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHCCC
GFKVSELSFQEKKLANDGTLKLLLSTNDNESIECVGIPTEKRLTACLSSQVGCPMDCKFC
CCEEHHCCHHHHHCCCCCEEEEEEECCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEE
ATGKEGLKRSLKASEILDQILFIENEMNRKVTNIVFMGMGEPLLNIDELLLSIRSINEDF
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCC
QISQRKITVSTVAVPKMMSKLSARSFQILGNCQFTLAISLHASNQKTRETIIPSAKNYEI
EECCEEEEEHHHHHHHHHHHHHHCCEEEEECEEEEEEEEEECCCCHHHHHHCCCCCCCHH
KNIIEDSKQFVKDTGRRVSFEYLMLSGVNDKLEHANELSNLLRGFQCHVNLIQYNQIDEV
HHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCHH
EFKRASLKNLQLFQSRLSNNGITVSFRKSRGLDKNAACGQLRQNARNK
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA