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The map label for this gene is purB [H]

Identifier: 78779942

GI number: 78779942

Start: 1449788

End: 1451083

Strand: Direct

Name: purB [H]

Synonym: PMT9312_1558

Alternate gene names: 78779942

Gene position: 1449788-1451083 (Clockwise)

Preceding gene: 78779940

Following gene: 78779943

Centisome position: 84.82

GC content: 34.57

Gene sequence:

>1296_bases
GTGATCGAGCGTTACACATTACCCGAAATGGGGGAAATCTGGACTGAAAGCGCAAAATTCCAGAGTTGGCTTAAGGTTGA
AATAGCTGCATGTGAAGCAAATTTTTCCCTCGGGAAAATTCCTGAGAATGCAATGAAAGAGATACGTTCAAATGCAAAGT
TTGATGAATCTAGAATTACAGAAATTGAGAAAGAAGTTAAACATGATGTCATAGCATTTCTTACAAGCGTTAATGAATTT
GTAGGAGATTCTGGAAGATACATACATGTTGGTATGACCAGTAGTGATGTACTTGATTCTGGCTTATCTCTTCAGTTAAA
AGATTCTTGCGAATTGTTATTAGAAGAAATTGAGAAATTAGAAAATGAAGTCAGATTATTAGCAAGGAAGCATAAAAATA
CATTAATGATTGGCAGATCTCATGCAATTCATGGGGAGCCAATTTCCTTCGGCTTTAAACTTGCTGGATGGTTAGCAGAA
ATAATAAGGAACAAAAAAAGATTGTTAACACTTAAAGAATCTGTAGCAATTGGACAAATAAGTGGTGCAATGGGAACTTA
TGCAAATACGAATCCCAAAGTAGAACAAATAACTTGTGATTTACTCGACTTAAAACCAGATACAGCAAGTACGCAGGTTA
TATCGAGAGACAGACATGCAGAATATGTGCAAACTATTGCACTAGTTGGCGCTTCTCTAGATAGATTCGCAACTGAAATC
AGAAATTTACAAAGAACTGATGTTTTAGAAGTTGAGGAGGGTTTTACAAAAGGGCAAAAAGGAAGTTCTGCCATGCCTCA
TAAAAGAAATCCTATTAGAAGTGAAAGAGTAAGTGGTTTAGCAAGAATTTTGAGGAGTTACGTCTTAACAGCACTGGAAA
ATGTTCCACTTTGGCACGAGAGAGATATAAGCCATAGTTCAAATGAACGTATCATGTTACCTGACGTATCAATCTGTTTG
CATTTTATGCTCAGGGAAATGAAAGATATAGTAAGCAATTTGGAAGTTTATCCAAAAAATATGCTCAAAAATTTAAATAT
ATATGGTGGTGTAATCTTTAGTCAGAAAGTTTTACTTTTGCTTGTAGAGAAGGGCTTGTCTAGAGAAAAAGCTTATAGCT
TAGTACAAAAAAATGCGCATCAGGCCTGGAATACTCAGGATGGGAATTTCAAACAAAATATAGAAAGAGATAATGAAATA
ATGGATTTTATTGATAAAAGTGACTTAGAAGAATGTTTTAATCCTTCAATTCATCTCAATAATTTAAGTGTAATATGGGA
GAAGTTAGGTATCTAG

Upstream 100 bases:

>100_bases
AATAAGCAGGCAAATATGTTCAATGGCAAGTACATCTTTAAGGTATAGGGCAATAATTCGATTTTGGTTATAAAGTTTAA
ATTGATAATTAATAAAAATT

Downstream 100 bases:

>100_bases
GATTACTGAAAACCTTATCTAAGTTAAACCAGTGTTTTCAGAGGAATAATGACAAAAAACTTTAGGATTGAAAAAGATAG
TATGGGAACTATAAAGGTTC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYTLPEMGEIWTESAKFQSWLKVEIAACEANFSLGKIPENAMKEIRSNAKFDESRITEIEKEVKHDVIAFLTSVNEF
VGDSGRYIHVGMTSSDVLDSGLSLQLKDSCELLLEEIEKLENEVRLLARKHKNTLMIGRSHAIHGEPISFGFKLAGWLAE
IIRNKKRLLTLKESVAIGQISGAMGTYANTNPKVEQITCDLLDLKPDTASTQVISRDRHAEYVQTIALVGASLDRFATEI
RNLQRTDVLEVEEGFTKGQKGSSAMPHKRNPIRSERVSGLARILRSYVLTALENVPLWHERDISHSSNERIMLPDVSICL
HFMLREMKDIVSNLEVYPKNMLKNLNIYGGVIFSQKVLLLLVEKGLSREKAYSLVQKNAHQAWNTQDGNFKQNIERDNEI
MDFIDKSDLEECFNPSIHLNNLSVIWEKLGI

Sequences:

>Translated_431_residues
MIERYTLPEMGEIWTESAKFQSWLKVEIAACEANFSLGKIPENAMKEIRSNAKFDESRITEIEKEVKHDVIAFLTSVNEF
VGDSGRYIHVGMTSSDVLDSGLSLQLKDSCELLLEEIEKLENEVRLLARKHKNTLMIGRSHAIHGEPISFGFKLAGWLAE
IIRNKKRLLTLKESVAIGQISGAMGTYANTNPKVEQITCDLLDLKPDTASTQVISRDRHAEYVQTIALVGASLDRFATEI
RNLQRTDVLEVEEGFTKGQKGSSAMPHKRNPIRSERVSGLARILRSYVLTALENVPLWHERDISHSSNERIMLPDVSICL
HFMLREMKDIVSNLEVYPKNMLKNLNIYGGVIFSQKVLLLLVEKGLSREKAYSLVQKNAHQAWNTQDGNFKQNIERDNEI
MDFIDKSDLEECFNPSIHLNNLSVIWEKLGI
>Mature_431_residues
MIERYTLPEMGEIWTESAKFQSWLKVEIAACEANFSLGKIPENAMKEIRSNAKFDESRITEIEKEVKHDVIAFLTSVNEF
VGDSGRYIHVGMTSSDVLDSGLSLQLKDSCELLLEEIEKLENEVRLLARKHKNTLMIGRSHAIHGEPISFGFKLAGWLAE
IIRNKKRLLTLKESVAIGQISGAMGTYANTNPKVEQITCDLLDLKPDTASTQVISRDRHAEYVQTIALVGASLDRFATEI
RNLQRTDVLEVEEGFTKGQKGSSAMPHKRNPIRSERVSGLARILRSYVLTALENVPLWHERDISHSSNERIMLPDVSICL
HFMLREMKDIVSNLEVYPKNMLKNLNIYGGVIFSQKVLLLLVEKGLSREKAYSLVQKNAHQAWNTQDGNFKQNIERDNEI
MDFIDKSDLEECFNPSIHLNNLSVIWEKLGI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=434, Percent_Identity=26.9585253456221, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI183227688, Length=383, Percent_Identity=27.4151436031332, Blast_Score=151, Evalue=1e-36,
Organism=Escherichia coli, GI1787376, Length=323, Percent_Identity=26.3157894736842, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17508577, Length=392, Percent_Identity=28.3163265306122, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI32564234, Length=334, Percent_Identity=28.7425149700599, Blast_Score=122, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6323391, Length=443, Percent_Identity=25.5079006772009, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24647570, Length=382, Percent_Identity=26.1780104712042, Blast_Score=125, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48940; Mature: 48940

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYTLPEMGEIWTESAKFQSWLKVEIAACEANFSLGKIPENAMKEIRSNAKFDESRIT
CCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHH
EIEKEVKHDVIAFLTSVNEFVGDSGRYIHVGMTSSDVLDSGLSLQLKDSCELLLEEIEKL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCEEEHHHHHHHHHHHHHH
ENEVRLLARKHKNTLMIGRSHAIHGEPISFGFKLAGWLAEIIRNKKRLLTLKESVAIGQI
HHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
SGAMGTYANTNPKVEQITCDLLDLKPDTASTQVISRDRHAEYVQTIALVGASLDRFATEI
CCCCCCCCCCCCCHHHEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQRTDVLEVEEGFTKGQKGSSAMPHKRNPIRSERVSGLARILRSYVLTALENVPLWHE
HHCHHCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RDISHSSNERIMLPDVSICLHFMLREMKDIVSNLEVYPKNMLKNLNIYGGVIFSQKVLLL
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHH
LVEKGLSREKAYSLVQKNAHQAWNTQDGNFKQNIERDNEIMDFIDKSDLEECFNPSIHLN
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
NLSVIWEKLGI
HHHHHHHHHCC
>Mature Secondary Structure
MIERYTLPEMGEIWTESAKFQSWLKVEIAACEANFSLGKIPENAMKEIRSNAKFDESRIT
CCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHH
EIEKEVKHDVIAFLTSVNEFVGDSGRYIHVGMTSSDVLDSGLSLQLKDSCELLLEEIEKL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCEEEHHHHHHHHHHHHHH
ENEVRLLARKHKNTLMIGRSHAIHGEPISFGFKLAGWLAEIIRNKKRLLTLKESVAIGQI
HHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
SGAMGTYANTNPKVEQITCDLLDLKPDTASTQVISRDRHAEYVQTIALVGASLDRFATEI
CCCCCCCCCCCCCHHHEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQRTDVLEVEEGFTKGQKGSSAMPHKRNPIRSERVSGLARILRSYVLTALENVPLWHE
HHCHHCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RDISHSSNERIMLPDVSICLHFMLREMKDIVSNLEVYPKNMLKNLNIYGGVIFSQKVLLL
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHH
LVEKGLSREKAYSLVQKNAHQAWNTQDGNFKQNIERDNEIMDFIDKSDLEECFNPSIHLN
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
NLSVIWEKLGI
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]