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The map label for this gene is purD [H]

Identifier: 78779822

GI number: 78779822

Start: 1350717

End: 1352048

Strand: Direct

Name: purD [H]

Synonym: PMT9312_1438

Alternate gene names: 78779822

Gene position: 1350717-1352048 (Clockwise)

Preceding gene: 78779814

Following gene: 78779823

Centisome position: 79.03

GC content: 31.68

Gene sequence:

>1332_bases
ATGAGTATAAATTCAACAAGTTCTAAAGACTACGGTAGATTAGAAAATGTTTTAATAATTGGCAATGGCGGGAGAGAAAA
TTCTTTGGCTTGGGCTATTCAAAAAAATGAATTAGTTAAAAAAGTTTATTTAATCCCTGGTAACGCCGGCTCAGAAAGAA
TAAATAAATGTGAAAGAATAAGAATTGACATAAACAATAAAAATAAATTAGTCGAAAAACTTGATTTTTTAAAAATAAAT
TTAATTGTAATAGGACCTGAAGTACCTTTAGCAAATGGATTAGCCGATTTTCTTCGCAAAAAAGACTATAAAGTATTTGG
CCCTGGTAAAGATGGAGCAAGATTGGAATATAGCAAATCTTGGGCAAAGGAATTTATGCGAGACGCAAATATTCCAACTG
CAAAATTTTGGACCGTCAATTCTCTTGAAGAAGCCACAAAAATTATCAATTCATCATCAATACCACTGGTAGTCAAAGCA
GATGGTCTAGCGTCAGGAAAAGGTGTTTTTATTCCCGCGTCAAAAGATGAATGTTATAGAGCGGCAGAATCCATATTTAA
TGGTAAATTTGGAAAATCTGGGGATATTGTAGTTCTAGAAGAAAAAATTCAGGGTCCAGAAGTTTCAGTTTTTGCTCTAT
GTGATGGGAAGAGATACATATTACTTCCAACTGCACAAGATCACAAACGCCTAAATGAGCAAGATAAAGGTCCAAATACA
GGTGGAATGGGAGCATATTCTCCTGCTCCACTTTTAACAAAAGATTACCTTGATAGGATAATCAAAGAGATAATTGAACC
GACAATAGATGAACTAAATAAAAAAAATATTGATTATAAAGGCGTAATTTATTTTGGATTAATGATCACAAAATATGGGC
CGAAAGTTATAGAATACAATTGCAGATTTGGAGATCCAGAATGTCAAACAATAATGCCTTTAATGGATCAAGATTTTGTA
TTTCTTTTAGAAAAATGCTCAATGGGAAATTTAACAGGTGATGAAAAAATTGATAATCCTGATAAGGTTAGTGGTTGCGT
AATAGCGACCTCAAAAGGATACCCACAGGAATATTTAACTGGATTTCCAATAAAAATAGGAAAGATTGACTCAAGTGATT
GTCAAATTTTTGACTCAGGTACTTGTTTAAATAAAAATGGAGAATTATTAACTGATGGAGGAAGAGTACTAAGTATTGTA
TGTCAAGAAAAAGATTTTGATATGGTTTTTGAAAAAGCATATAAAAATCTTAAAGAAATAAATTTTGAGGGAATTTACTT
TAGAAATGATATAGGGCATCAAGTTAGAAAAAAATTTTCTAGGGAGAATTAG

Upstream 100 bases:

>100_bases
AGATTTGTAATTAACATATTTAGTAGAGATCAACTTTTTATAGTTTATTTCATTTTAACTGATTATTCATCGAAACCTAA
TAGCCTTCAAAAACAAATTA

Downstream 100 bases:

>100_bases
CTTATGGTAGATACCAAGAACCAAGATAGTAGAGATTATAATAGTACCAGTGATAATGAAGATTTTCATAACAACTATTG
GACTAATAGGATAATTGCTT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSINSTSSKDYGRLENVLIIGNGGRENSLAWAIQKNELVKKVYLIPGNAGSERINKCERIRIDINNKNKLVEKLDFLKIN
LIVIGPEVPLANGLADFLRKKDYKVFGPGKDGARLEYSKSWAKEFMRDANIPTAKFWTVNSLEEATKIINSSSIPLVVKA
DGLASGKGVFIPASKDECYRAAESIFNGKFGKSGDIVVLEEKIQGPEVSVFALCDGKRYILLPTAQDHKRLNEQDKGPNT
GGMGAYSPAPLLTKDYLDRIIKEIIEPTIDELNKKNIDYKGVIYFGLMITKYGPKVIEYNCRFGDPECQTIMPLMDQDFV
FLLEKCSMGNLTGDEKIDNPDKVSGCVIATSKGYPQEYLTGFPIKIGKIDSSDCQIFDSGTCLNKNGELLTDGGRVLSIV
CQEKDFDMVFEKAYKNLKEINFEGIYFRNDIGHQVRKKFSREN

Sequences:

>Translated_443_residues
MSINSTSSKDYGRLENVLIIGNGGRENSLAWAIQKNELVKKVYLIPGNAGSERINKCERIRIDINNKNKLVEKLDFLKIN
LIVIGPEVPLANGLADFLRKKDYKVFGPGKDGARLEYSKSWAKEFMRDANIPTAKFWTVNSLEEATKIINSSSIPLVVKA
DGLASGKGVFIPASKDECYRAAESIFNGKFGKSGDIVVLEEKIQGPEVSVFALCDGKRYILLPTAQDHKRLNEQDKGPNT
GGMGAYSPAPLLTKDYLDRIIKEIIEPTIDELNKKNIDYKGVIYFGLMITKYGPKVIEYNCRFGDPECQTIMPLMDQDFV
FLLEKCSMGNLTGDEKIDNPDKVSGCVIATSKGYPQEYLTGFPIKIGKIDSSDCQIFDSGTCLNKNGELLTDGGRVLSIV
CQEKDFDMVFEKAYKNLKEINFEGIYFRNDIGHQVRKKFSREN
>Mature_442_residues
SINSTSSKDYGRLENVLIIGNGGRENSLAWAIQKNELVKKVYLIPGNAGSERINKCERIRIDINNKNKLVEKLDFLKINL
IVIGPEVPLANGLADFLRKKDYKVFGPGKDGARLEYSKSWAKEFMRDANIPTAKFWTVNSLEEATKIINSSSIPLVVKAD
GLASGKGVFIPASKDECYRAAESIFNGKFGKSGDIVVLEEKIQGPEVSVFALCDGKRYILLPTAQDHKRLNEQDKGPNTG
GMGAYSPAPLLTKDYLDRIIKEIIEPTIDELNKKNIDYKGVIYFGLMITKYGPKVIEYNCRFGDPECQTIMPLMDQDFVF
LLEKCSMGNLTGDEKIDNPDKVSGCVIATSKGYPQEYLTGFPIKIGKIDSSDCQIFDSGTCLNKNGELLTDGGRVLSIVC
QEKDFDMVFEKAYKNLKEINFEGIYFRNDIGHQVRKKFSREN

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=42.688679245283, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI4503915, Length=423, Percent_Identity=42.789598108747, Blast_Score=343, Evalue=1e-94,
Organism=Homo sapiens, GI209869995, Length=423, Percent_Identity=42.789598108747, Blast_Score=343, Evalue=1e-94,
Organism=Homo sapiens, GI209869993, Length=423, Percent_Identity=42.789598108747, Blast_Score=343, Evalue=1e-94,
Organism=Escherichia coli, GI1790438, Length=424, Percent_Identity=38.6792452830189, Blast_Score=305, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI17567511, Length=427, Percent_Identity=41.4519906323185, Blast_Score=289, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6321203, Length=444, Percent_Identity=37.1621621621622, Blast_Score=281, Evalue=2e-76,
Organism=Drosophila melanogaster, GI62471619, Length=435, Percent_Identity=40, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24582400, Length=433, Percent_Identity=40.1847575057737, Blast_Score=295, Evalue=6e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 49519; Mature: 49388

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSINSTSSKDYGRLENVLIIGNGGRENSLAWAIQKNELVKKVYLIPGNAGSERINKCERI
CCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCEEEEEEEECCCCCHHHHCCCEEE
RIDINNKNKLVEKLDFLKINLIVIGPEVPLANGLADFLRKKDYKVFGPGKDGARLEYSKS
EEEECCCHHHHHHHCEEEEEEEEECCCCCCHHHHHHHHHHCCCEEECCCCCCCEEEHHHH
WAKEFMRDANIPTAKFWTVNSLEEATKIINSSSIPLVVKADGLASGKGVFIPASKDECYR
HHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCEEEEECCCCCCCEEEEECCHHHHHH
AAESIFNGKFGKSGDIVVLEEKIQGPEVSVFALCDGKRYILLPTAQDHKRLNEQDKGPNT
HHHHHHCCCCCCCCCEEEEECCCCCCCEEEEEEECCCEEEEECCCHHHHHCCCCCCCCCC
GGMGAYSPAPLLTKDYLDRIIKEIIEPTIDELNKKNIDYKGVIYFGLMITKYGPKVIEYN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEHCCCCCEEEEEE
CRFGDPECQTIMPLMDQDFVFLLEKCSMGNLTGDEKIDNPDKVSGCVIATSKGYPQEYLT
CCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHC
GFPIKIGKIDSSDCQIFDSGTCLNKNGELLTDGGRVLSIVCQEKDFDMVFEKAYKNLKEI
CCCEEEEECCCCCCEEEECCCEECCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHC
NFEGIYFRNDIGHQVRKKFSREN
CCCEEEEECCHHHHHHHHHCCCC
>Mature Secondary Structure 
SINSTSSKDYGRLENVLIIGNGGRENSLAWAIQKNELVKKVYLIPGNAGSERINKCERI
CCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCEEEEEEEECCCCCHHHHCCCEEE
RIDINNKNKLVEKLDFLKINLIVIGPEVPLANGLADFLRKKDYKVFGPGKDGARLEYSKS
EEEECCCHHHHHHHCEEEEEEEEECCCCCCHHHHHHHHHHCCCEEECCCCCCCEEEHHHH
WAKEFMRDANIPTAKFWTVNSLEEATKIINSSSIPLVVKADGLASGKGVFIPASKDECYR
HHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCEEEEECCCCCCCEEEEECCHHHHHH
AAESIFNGKFGKSGDIVVLEEKIQGPEVSVFALCDGKRYILLPTAQDHKRLNEQDKGPNT
HHHHHHCCCCCCCCCEEEEECCCCCCCEEEEEEECCCEEEEECCCHHHHHCCCCCCCCCC
GGMGAYSPAPLLTKDYLDRIIKEIIEPTIDELNKKNIDYKGVIYFGLMITKYGPKVIEYN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEHCCCCCEEEEEE
CRFGDPECQTIMPLMDQDFVFLLEKCSMGNLTGDEKIDNPDKVSGCVIATSKGYPQEYLT
CCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHC
GFPIKIGKIDSSDCQIFDSGTCLNKNGELLTDGGRVLSIVCQEKDFDMVFEKAYKNLKEI
CCCEEEEECCCCCCEEEECCCEECCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHC
NFEGIYFRNDIGHQVRKKFSREN
CCCEEEEECCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]