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The map label for this gene is frdA [C]

Identifier: 78779738

GI number: 78779738

Start: 1269888

End: 1271636

Strand: Direct

Name: frdA [C]

Synonym: PMT9312_1354

Alternate gene names: 78779738

Gene position: 1269888-1271636 (Clockwise)

Preceding gene: 78779737

Following gene: 78779739

Centisome position: 74.3

GC content: 31.33

Gene sequence:

>1749_bases
ATGAAAAGTATTGTTCAAGAGAGATATGAGATACTTGTTATTGGGGCAGGTGGTGCTGGTTTAAGAGCTGCTATAGAGGC
AAAATCATTAGGTAAAGAAGTTTTAGTTGTTGGGAAAAGAAGTAAATTCGATGCTCATACTGCTTTAGCTGCAGGAGGCA
TAAATGCAGTTTTTGGTAATTTAGATCCTAATGATTCTAAAGAGCAACATTTTGCAGATACATATCTTGAGGGCTATGGA
ATAGGGGATCCTGATATTATTGAGATAATGGTAAATGAATCACCAAAAAGAGTTAAAGAAATAGATGAATGGGGAGCAAA
CTTTAAAAGATTAAAAAATGGTAAATTAGATCAGAGATTTTTTGGCGCTCATACTTATCGGAGAACCTGTTACTCAGGTG
ATTATACAGGCAAATCTATCCTTAATGCATTATTAAATAAAGCTGATTCTATGGCAATACCCATAATAGATACTACTTAC
ATAACAGAACTCATAATCTATGAAGATCGATGTATAGGTGCAATGTCTTTTGATATTAAATCTGGAGAGAAAAAAATAAT
CCTATCCAAAGCCATCATTATATGCACAGGTGGTCATACTGGGATTTGGAAAAGGAACTCATCGCGAAAAGATGAAAATA
ATGGAGATGGTCTTGCTCTAGCAATAAATGCAGGATGCGAACTAATTGATATGGAAATGGTTCAATTTCATCCAACTGGG
ATGATCTTTCCTGAAGAGATTTCTGGGAATCTAGTTACTGAAGCAGTTAGGGGAGAGGGTGGAAAACTTTACAATAATAA
AGGGCAAAGATTTATGGAGTCTTATGATAAAGAGAGGTTAGAGTTATCCACAAGAGATAAAGTTGCAATAGCAAATTACA
CTGAAATAAAAGACGGTAGGGGTACTCCTAGAGGTGGCGTATATCTAGATATCAGTCATATAGAAAAAGATAAAATACTT
GAAAAACTACCAAATATATTTAAACAGTTCAAAGATTATCAAAATATTGATATTTCAAAAGAGCCAATGGAAGTTGCGCC
TACAGCTCATTATTCTATGGGGGGCATTAAAGTTTGCGCAATTAAGCATTCAACATCAATAAGTGGACTTTTTGCAGCAG
GAGAAGTAGCTGGAGGACTTCACGGAGCTAATAGATTAGGTGGCAACTCCTTAGCAGAAATTCTAGTTTTTGGTAAAAAA
GCAGGAAAGTCTGCCTCAGAATATTCATCAACAGTAAATTTCACAAATGAAATACCTACAAATATCATCAATAAAATTGA
TCATAATATTAATCAATTAATGTTTAAAGAAATGAAAAATCCAAAATTATTTTTAAATAATTTAAAAGACATAATGTGGC
AATATTGTGGAGTTATAAAGAATAAAAAGGGCTTAGAATTTGGGTTAAATAAAATAGATGAGATGGGAGAAAAATTTAAA
AAATCTAGTATAAAAAAGACAAAAAACAAACCTGATTTTCTAGTAGATTTGTTCAACATAAAGTTTTCTATTTTAGTTAG
CAAACTAACACTAATTAGTACTATTCATAGAGAGGAAAGTAGAGGTGCCCATCAGAGATCTGATTTTAAAGATTTAAACA
AATCAAGAAACTATAATATAATTTGTAAAATTACAAATAGAAAAATATCTATTGAAAAAAAACCTACAAAAATCCTGAAA
AAAGATATTGTAAAATTTATCAAGAATGATTTAAGAAATAAAAATATAGGTATAAAGTTACTTGAATGA

Upstream 100 bases:

>100_bases
ACACTTATAAATAAAAATGATTTAATAGAATAATCATTTTTTTTTATAAAAACAAATTCTCTAAAATAAGAATTTTATTT
ATCACAAAAAATTATTACTT

Downstream 100 bases:

>100_bases
TATTTTTTTTACATTTATTGCATTACTCTAATCAAAAAATTCTTTATAAGTTATACAATTATAATGATAAAATATTCTAA
TATTCTTTATTTTTTAAAAG

Product: succinate dehydrogenase

Products: fumarate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MKSIVQERYEILVIGAGGAGLRAAIEAKSLGKEVLVVGKRSKFDAHTALAAGGINAVFGNLDPNDSKEQHFADTYLEGYG
IGDPDIIEIMVNESPKRVKEIDEWGANFKRLKNGKLDQRFFGAHTYRRTCYSGDYTGKSILNALLNKADSMAIPIIDTTY
ITELIIYEDRCIGAMSFDIKSGEKKIILSKAIIICTGGHTGIWKRNSSRKDENNGDGLALAINAGCELIDMEMVQFHPTG
MIFPEEISGNLVTEAVRGEGGKLYNNKGQRFMESYDKERLELSTRDKVAIANYTEIKDGRGTPRGGVYLDISHIEKDKIL
EKLPNIFKQFKDYQNIDISKEPMEVAPTAHYSMGGIKVCAIKHSTSISGLFAAGEVAGGLHGANRLGGNSLAEILVFGKK
AGKSASEYSSTVNFTNEIPTNIINKIDHNINQLMFKEMKNPKLFLNNLKDIMWQYCGVIKNKKGLEFGLNKIDEMGEKFK
KSSIKKTKNKPDFLVDLFNIKFSILVSKLTLISTIHREESRGAHQRSDFKDLNKSRNYNIICKITNRKISIEKKPTKILK
KDIVKFIKNDLRNKNIGIKLLE

Sequences:

>Translated_582_residues
MKSIVQERYEILVIGAGGAGLRAAIEAKSLGKEVLVVGKRSKFDAHTALAAGGINAVFGNLDPNDSKEQHFADTYLEGYG
IGDPDIIEIMVNESPKRVKEIDEWGANFKRLKNGKLDQRFFGAHTYRRTCYSGDYTGKSILNALLNKADSMAIPIIDTTY
ITELIIYEDRCIGAMSFDIKSGEKKIILSKAIIICTGGHTGIWKRNSSRKDENNGDGLALAINAGCELIDMEMVQFHPTG
MIFPEEISGNLVTEAVRGEGGKLYNNKGQRFMESYDKERLELSTRDKVAIANYTEIKDGRGTPRGGVYLDISHIEKDKIL
EKLPNIFKQFKDYQNIDISKEPMEVAPTAHYSMGGIKVCAIKHSTSISGLFAAGEVAGGLHGANRLGGNSLAEILVFGKK
AGKSASEYSSTVNFTNEIPTNIINKIDHNINQLMFKEMKNPKLFLNNLKDIMWQYCGVIKNKKGLEFGLNKIDEMGEKFK
KSSIKKTKNKPDFLVDLFNIKFSILVSKLTLISTIHREESRGAHQRSDFKDLNKSRNYNIICKITNRKISIEKKPTKILK
KDIVKFIKNDLRNKNIGIKLLE
>Mature_582_residues
MKSIVQERYEILVIGAGGAGLRAAIEAKSLGKEVLVVGKRSKFDAHTALAAGGINAVFGNLDPNDSKEQHFADTYLEGYG
IGDPDIIEIMVNESPKRVKEIDEWGANFKRLKNGKLDQRFFGAHTYRRTCYSGDYTGKSILNALLNKADSMAIPIIDTTY
ITELIIYEDRCIGAMSFDIKSGEKKIILSKAIIICTGGHTGIWKRNSSRKDENNGDGLALAINAGCELIDMEMVQFHPTG
MIFPEEISGNLVTEAVRGEGGKLYNNKGQRFMESYDKERLELSTRDKVAIANYTEIKDGRGTPRGGVYLDISHIEKDKIL
EKLPNIFKQFKDYQNIDISKEPMEVAPTAHYSMGGIKVCAIKHSTSISGLFAAGEVAGGLHGANRLGGNSLAEILVFGKK
AGKSASEYSSTVNFTNEIPTNIINKIDHNINQLMFKEMKNPKLFLNNLKDIMWQYCGVIKNKKGLEFGLNKIDEMGEKFK
KSSIKKTKNKPDFLVDLFNIKFSILVSKLTLISTIHREESRGAHQRSDFKDLNKSRNYNIICKITNRKISIEKKPTKILK
KDIVKFIKNDLRNKNIGIKLLE

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=518, Percent_Identity=32.4324324324324, Blast_Score=251, Evalue=1e-66,
Organism=Escherichia coli, GI1790597, Length=521, Percent_Identity=33.3973128598848, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1786942, Length=598, Percent_Identity=31.6053511705686, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1788928, Length=551, Percent_Identity=30.1270417422868, Blast_Score=234, Evalue=9e-63,
Organism=Caenorhabditis elegans, GI17505833, Length=532, Percent_Identity=31.5789473684211, Blast_Score=261, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI17550100, Length=532, Percent_Identity=31.390977443609, Blast_Score=251, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI71986328, Length=71, Percent_Identity=53.5211267605634, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322701, Length=553, Percent_Identity=32.368896925859, Blast_Score=250, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6322416, Length=550, Percent_Identity=32.1818181818182, Blast_Score=246, Evalue=5e-66,
Organism=Saccharomyces cerevisiae, GI6322511, Length=474, Percent_Identity=28.4810126582279, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6320788, Length=473, Percent_Identity=28.9640591966173, Blast_Score=131, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17137288, Length=555, Percent_Identity=32.6126126126126, Blast_Score=259, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24655642, Length=555, Percent_Identity=32.6126126126126, Blast_Score=259, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24655647, Length=555, Percent_Identity=32.6126126126126, Blast_Score=259, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24663005, Length=555, Percent_Identity=32.2522522522522, Blast_Score=249, Evalue=5e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1

Molecular weight: Translated: 64981; Mature: 64981

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIVQERYEILVIGAGGAGLRAAIEAKSLGKEVLVVGKRSKFDAHTALAAGGINAVFGN
CCHHHHCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCEEECC
LDPNDSKEQHFADTYLEGYGIGDPDIIEIMVNESPKRVKEIDEWGANFKRLKNGKLDQRF
CCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCHHHHHCCCCCCHHH
FGAHTYRRTCYSGDYTGKSILNALLNKADSMAIPIIDTTYITELIIYEDRCIGAMSFDIK
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEEEHHHHHHHEEECCCCCEEEEEECC
SGEKKIILSKAIIICTGGHTGIWKRNSSRKDENNGDGLALAINAGCELIDMEMVQFHPTG
CCCCEEEEEEEEEEEECCCCCEECCCCCCCCCCCCCEEEEEEECCCCEEEEHHHEECCCC
MIFPEEISGNLVTEAVRGEGGKLYNNKGQRFMESYDKERLELSTRDKVAIANYTEIKDGR
CCCHHHHCCCHHHHHHHCCCCCEECCCHHHHHHHHCHHHHEECCCCCEEEECCCCCCCCC
GTPRGGVYLDISHIEKDKILEKLPNIFKQFKDYQNIDISKEPMEVAPTAHYSMGGIKVCA
CCCCCCEEEEHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
IKHSTSISGLFAAGEVAGGLHGANRLGGNSLAEILVFGKKAGKSASEYSSTVNFTNEIPT
EECCCCCCCEEEHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCH
NIINKIDHNINQLMFKEMKNPKLFLNNLKDIMWQYCGVIKNKKGLEFGLNKIDEMGEKFK
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
KSSIKKTKNKPDFLVDLFNIKFSILVSKLTLISTIHREESRGAHQRSDFKDLNKSRNYNI
HHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEE
ICKITNRKISIEKKPTKILKKDIVKFIKNDLRNKNIGIKLLE
EEEEECCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEC
>Mature Secondary Structure
MKSIVQERYEILVIGAGGAGLRAAIEAKSLGKEVLVVGKRSKFDAHTALAAGGINAVFGN
CCHHHHCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCEEECC
LDPNDSKEQHFADTYLEGYGIGDPDIIEIMVNESPKRVKEIDEWGANFKRLKNGKLDQRF
CCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCHHHHHCCCCCCHHH
FGAHTYRRTCYSGDYTGKSILNALLNKADSMAIPIIDTTYITELIIYEDRCIGAMSFDIK
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEEEHHHHHHHEEECCCCCEEEEEECC
SGEKKIILSKAIIICTGGHTGIWKRNSSRKDENNGDGLALAINAGCELIDMEMVQFHPTG
CCCCEEEEEEEEEEEECCCCCEECCCCCCCCCCCCCEEEEEEECCCCEEEEHHHEECCCC
MIFPEEISGNLVTEAVRGEGGKLYNNKGQRFMESYDKERLELSTRDKVAIANYTEIKDGR
CCCHHHHCCCHHHHHHHCCCCCEECCCHHHHHHHHCHHHHEECCCCCEEEECCCCCCCCC
GTPRGGVYLDISHIEKDKILEKLPNIFKQFKDYQNIDISKEPMEVAPTAHYSMGGIKVCA
CCCCCCEEEEHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
IKHSTSISGLFAAGEVAGGLHGANRLGGNSLAEILVFGKKAGKSASEYSSTVNFTNEIPT
EECCCCCCCEEEHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCH
NIINKIDHNINQLMFKEMKNPKLFLNNLKDIMWQYCGVIKNKKGLEFGLNKIDEMGEKFK
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
KSSIKKTKNKPDFLVDLFNIKFSILVSKLTLISTIHREESRGAHQRSDFKDLNKSRNYNI
HHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEE
ICKITNRKISIEKKPTKILKKDIVKFIKNDLRNKNIGIKLLE
EEEEECCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.02 {succinate}} 0.3 {ferricyanide}} 0.4 {fumarate}} [C]

Substrates: succinate; acceptor

Specific reaction: succinate + acceptor = fumarate + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]