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The map label for this gene is pnp [H]

Identifier: 78779675

GI number: 78779675

Start: 1198352

End: 1200517

Strand: Direct

Name: pnp [H]

Synonym: PMT9312_1292

Alternate gene names: 78779675

Gene position: 1198352-1200517 (Clockwise)

Preceding gene: 78779674

Following gene: 78779677

Centisome position: 70.11

GC content: 40.03

Gene sequence:

>2166_bases
GTGGAAGGACAAAATAAGTCGATCACGTTTGACGGACGAGAGATACGACTAACTACAGGACTATATGCTCCTCAAGCAAA
TGGATCAGTAATGATTGAGTGTGGGGACACCTCGTTATTAGTTACAGCAACAAAAACGACTAAGAAAGAGGTTGCAGACT
TTCTACCTCTTATATGCGACTATGAGGAAAAACTTTATGCTGCAGGGAGGATTCCAGGTGGTTTTATGAGGAGAGAGGGT
CGCCCTCCAGAAAGAGCGACTTTAATTTCAAGGTTGATTGATAGGCCAATGAGGCCGCTTTTCCCTTCATGGATGCGAGA
CGAGATACAAATAGTTGCTTCATGCCTTTCTTTAGATGAAAGGGTTCCAGCAGATGTTTTAGCTGTTACTGGAGCTTCAA
TAGCAACTTTACTTGGAGAGATACCATTTCATGGGCCAATGGCAGCTGTTAGAGTTGGGCTCATAGGAGATGATTTCATC
TTAAATCCAAGCTATAGAGAGATAGAGAAAGGAGATTTAGACATTGTTGTTGCAGGATCACCTGACGGCATTGTTATGAT
TGAAGCAGGTGCTAACCAATTATCAGAGCAAGATACTATTGAAGCTATAGATTTTGGTTATGAGTCAGTAACTGAACTAA
TTAAATCTCAAGTAGATTTACTAAAAGATTTAGGTATAAAACAGGTTAAGTCATCGGCTCCCGAAGAAGATAATACATTG
CCCTCTTATTTAGAGAAGAATTGTACAAAGGGAATTGAGTTGGTTTTAAAGAAATTTGACCAGTCTAAAGATGAGAGAGA
TCTTGAACTCGAAAAAATAAAAGTTGATACTCAAGCTAAAATTGAATCTTTGAAAGATGATAACGAATTAAAAGTTCTTC
TTTCAGAGAATGATAAGTTGTTAAGTTCCGACTTCAAAAAACTTACAAAGAAATTAATGAGGTCCCAAATCATTAATGAT
GGCAAACGAGTTGATGGCCGTGAATTAGATGAAGTGAGAAAAATTTCAGCATCTGCTGGGATACTTCCAAAAAGGGTTCA
TGGTTCAGCCTTATTTCAAAGAGGGTTGACTCAAGTCTTATCAACGACAACTTTGGGAACGCCAAGTGACGCACAGGAAA
TGGACGACCTTAATCCAAGCACTGAAAAAACTTATCTGCATCACTATAATTTCCCACCTTTTTCAGTTGGGGAAACCAGA
CCCATGAGAACCCCTGGCAGAAGAGAGATTGGCCATGGAGCTTTAGCAGAGAGAGCGATAATTCCTGTTCTTCCGGGGAA
AGAAACATTCCCATATGTATTAAGGGTTGTTAGCGAAGTTTTAAGTTCGAACGGATCAACTTCCATGGGTTCTGTATGTG
GAAGTACACTTTCGTTATTAGATGCAGGAGTTCCTTTAAAAGCCCTTGTCAGTGGTACTGCTATGGGTTTAATCAAAGAA
GGGAAGGAGATCAGAATTCTCACGGATATTCAAGGGATCGAGGACTTTCTTGGGGACATGGACTTTAAAGTTGCTGGTAC
AGAAAAGGGGATAACAGCATTACAAATGGATATGAAAATTACAGGTTTGTCCGTTTCCGTTATTTCTGATGCAATCAAAA
AAGCTCGTCCTGCAAGATTACACATCTTAGAAAAAATGCAAGAGGCAATAGATAAACCTCAAGAATCCTTATCTCCCCAC
GCACCAAGACTTTTAAGCTTTAGGATTGATCCAGAGCTCATTGGGACAGTCATTGGACCTGGTGGAAGAACCATTAAAGG
AATTACCGAAAGAACAAATACAAAAATCGATATTGAAGATGGGGGAATTGTAACCATCGCATCACATGATGGCGCTGCGG
CTGAAGAAGCGCAGAAGATAATAGAAGGATTAACTAGAAAAGTCCACGAAGGTGAAATTTTCACTGGAGTTGTCACTCGA
ATAATTCCGATTGGAGCATTTGTTGAAATTCTCCCTGGTAAGGAGGGAATGGTTCATATATCCCAATTATCTGAAGCGAG
AGTTGAAAGGGTGGAAGATGTTGTAAGACAAGGTGATGAAGTAACTGTAAGGGTTCGTGAAATTGATAGCAGAGGTAGGA
TCAATCTTACTTTGAGGGGAGTTTCACAAAATAGTGGGATGTCTTATCCTGAGCCAACACCTACGCCAGTAGCACCTTTA
AACTAA

Upstream 100 bases:

>100_bases
GGACAATTTTTTAAAATTTTTATAGCTTATCTAAATAATTTACTCAATATGTCCCTATAAAATGTAAGAATAAATTATGT
ATAGATTTATAATTTAAAAA

Downstream 100 bases:

>100_bases
TTTCCTAAGGATAAATTTTATATTCCTCAATAATTTCTTTTACTTGCGAGCAGATTCTTTTATGATTCTTCTTGTTATTA
GAGGCAATTATAATACCTGA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 721; Mature: 721

Protein sequence:

>721_residues
MEGQNKSITFDGREIRLTTGLYAPQANGSVMIECGDTSLLVTATKTTKKEVADFLPLICDYEEKLYAAGRIPGGFMRREG
RPPERATLISRLIDRPMRPLFPSWMRDEIQIVASCLSLDERVPADVLAVTGASIATLLGEIPFHGPMAAVRVGLIGDDFI
LNPSYREIEKGDLDIVVAGSPDGIVMIEAGANQLSEQDTIEAIDFGYESVTELIKSQVDLLKDLGIKQVKSSAPEEDNTL
PSYLEKNCTKGIELVLKKFDQSKDERDLELEKIKVDTQAKIESLKDDNELKVLLSENDKLLSSDFKKLTKKLMRSQIIND
GKRVDGRELDEVRKISASAGILPKRVHGSALFQRGLTQVLSTTTLGTPSDAQEMDDLNPSTEKTYLHHYNFPPFSVGETR
PMRTPGRREIGHGALAERAIIPVLPGKETFPYVLRVVSEVLSSNGSTSMGSVCGSTLSLLDAGVPLKALVSGTAMGLIKE
GKEIRILTDIQGIEDFLGDMDFKVAGTEKGITALQMDMKITGLSVSVISDAIKKARPARLHILEKMQEAIDKPQESLSPH
APRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIEDGGIVTIASHDGAAAEEAQKIIEGLTRKVHEGEIFTGVVTR
IIPIGAFVEILPGKEGMVHISQLSEARVERVEDVVRQGDEVTVRVREIDSRGRINLTLRGVSQNSGMSYPEPTPTPVAPL
N

Sequences:

>Translated_721_residues
MEGQNKSITFDGREIRLTTGLYAPQANGSVMIECGDTSLLVTATKTTKKEVADFLPLICDYEEKLYAAGRIPGGFMRREG
RPPERATLISRLIDRPMRPLFPSWMRDEIQIVASCLSLDERVPADVLAVTGASIATLLGEIPFHGPMAAVRVGLIGDDFI
LNPSYREIEKGDLDIVVAGSPDGIVMIEAGANQLSEQDTIEAIDFGYESVTELIKSQVDLLKDLGIKQVKSSAPEEDNTL
PSYLEKNCTKGIELVLKKFDQSKDERDLELEKIKVDTQAKIESLKDDNELKVLLSENDKLLSSDFKKLTKKLMRSQIIND
GKRVDGRELDEVRKISASAGILPKRVHGSALFQRGLTQVLSTTTLGTPSDAQEMDDLNPSTEKTYLHHYNFPPFSVGETR
PMRTPGRREIGHGALAERAIIPVLPGKETFPYVLRVVSEVLSSNGSTSMGSVCGSTLSLLDAGVPLKALVSGTAMGLIKE
GKEIRILTDIQGIEDFLGDMDFKVAGTEKGITALQMDMKITGLSVSVISDAIKKARPARLHILEKMQEAIDKPQESLSPH
APRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIEDGGIVTIASHDGAAAEEAQKIIEGLTRKVHEGEIFTGVVTR
IIPIGAFVEILPGKEGMVHISQLSEARVERVEDVVRQGDEVTVRVREIDSRGRINLTLRGVSQNSGMSYPEPTPTPVAPL
N
>Mature_721_residues
MEGQNKSITFDGREIRLTTGLYAPQANGSVMIECGDTSLLVTATKTTKKEVADFLPLICDYEEKLYAAGRIPGGFMRREG
RPPERATLISRLIDRPMRPLFPSWMRDEIQIVASCLSLDERVPADVLAVTGASIATLLGEIPFHGPMAAVRVGLIGDDFI
LNPSYREIEKGDLDIVVAGSPDGIVMIEAGANQLSEQDTIEAIDFGYESVTELIKSQVDLLKDLGIKQVKSSAPEEDNTL
PSYLEKNCTKGIELVLKKFDQSKDERDLELEKIKVDTQAKIESLKDDNELKVLLSENDKLLSSDFKKLTKKLMRSQIIND
GKRVDGRELDEVRKISASAGILPKRVHGSALFQRGLTQVLSTTTLGTPSDAQEMDDLNPSTEKTYLHHYNFPPFSVGETR
PMRTPGRREIGHGALAERAIIPVLPGKETFPYVLRVVSEVLSSNGSTSMGSVCGSTLSLLDAGVPLKALVSGTAMGLIKE
GKEIRILTDIQGIEDFLGDMDFKVAGTEKGITALQMDMKITGLSVSVISDAIKKARPARLHILEKMQEAIDKPQESLSPH
APRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIEDGGIVTIASHDGAAAEEAQKIIEGLTRKVHEGEIFTGVVTR
IIPIGAFVEILPGKEGMVHISQLSEARVERVEDVVRQGDEVTVRVREIDSRGRINLTLRGVSQNSGMSYPEPTPTPVAPL
N

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=742, Percent_Identity=35.44474393531, Blast_Score=437, Evalue=1e-122,
Organism=Escherichia coli, GI145693187, Length=701, Percent_Identity=45.2211126961484, Blast_Score=598, Evalue=1e-172,
Organism=Caenorhabditis elegans, GI115534063, Length=676, Percent_Identity=34.1715976331361, Blast_Score=352, Evalue=5e-97,
Organism=Drosophila melanogaster, GI281362905, Length=680, Percent_Identity=37.0588235294118, Blast_Score=422, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24651641, Length=680, Percent_Identity=37.0588235294118, Blast_Score=422, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24651643, Length=680, Percent_Identity=37.0588235294118, Blast_Score=422, Evalue=1e-118,
Organism=Drosophila melanogaster, GI161079377, Length=624, Percent_Identity=37.0192307692308, Blast_Score=387, Evalue=1e-107,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 78657; Mature: 78657

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGQNKSITFDGREIRLTTGLYAPQANGSVMIECGDTSLLVTATKTTKKEVADFLPLICD
CCCCCCCEEECCCEEEEEECEECCCCCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHC
YEEKLYAAGRIPGGFMRREGRPPERATLISRLIDRPMRPLFPSWMRDEIQIVASCLSLDE
CHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
RVPADVLAVTGASIATLLGEIPFHGPMAAVRVGLIGDDFILNPSYREIEKGDLDIVVAGS
CCCHHHHHHCCHHHHHHHHCCCCCCCHHHHEEEEECCCEEECCCHHHHCCCCEEEEEECC
PDGIVMIEAGANQLSEQDTIEAIDFGYESVTELIKSQVDLLKDLGIKQVKSSAPEEDNTL
CCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCC
PSYLEKNCTKGIELVLKKFDQSKDERDLELEKIKVDTQAKIESLKDDNELKVLLSENDKL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHCCCCCCEEEEECCCCHH
LSSDFKKLTKKLMRSQIINDGKRVDGRELDEVRKISASAGILPKRVHGSALFQRGLTQVL
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
STTTLGTPSDAQEMDDLNPSTEKTYLHHYNFPPFSVGETRPMRTPGRREIGHGALAERAI
HHCCCCCCCCHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHCCCCHHHHCCE
IPVLPGKETFPYVLRVVSEVLSSNGSTSMGSVCGSTLSLLDAGVPLKALVSGTAMGLIKE
EECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
GKEIRILTDIQGIEDFLGDMDFKVAGTEKGITALQMDMKITGLSVSVISDAIKKARPARL
CCEEEEEECHHHHHHHHCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHCCCHHH
HILEKMQEAIDKPQESLSPHAPRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIED
HHHHHHHHHHCCCHHHCCCCCCCEEEEEECHHHHHEEECCCCCEEECHHHCCCCEEEECC
GGIVTIASHDGAAAEEAQKIIEGLTRKVHEGEIFTGVVTRIIPIGAFVEILPGKEGMVHI
CCEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCCCCEEH
SQLSEARVERVEDVVRQGDEVTVRVREIDSRGRINLTLRGVSQNSGMSYPEPTPTPVAPL
HHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCC
N
C
>Mature Secondary Structure
MEGQNKSITFDGREIRLTTGLYAPQANGSVMIECGDTSLLVTATKTTKKEVADFLPLICD
CCCCCCCEEECCCEEEEEECEECCCCCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHC
YEEKLYAAGRIPGGFMRREGRPPERATLISRLIDRPMRPLFPSWMRDEIQIVASCLSLDE
CHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
RVPADVLAVTGASIATLLGEIPFHGPMAAVRVGLIGDDFILNPSYREIEKGDLDIVVAGS
CCCHHHHHHCCHHHHHHHHCCCCCCCHHHHEEEEECCCEEECCCHHHHCCCCEEEEEECC
PDGIVMIEAGANQLSEQDTIEAIDFGYESVTELIKSQVDLLKDLGIKQVKSSAPEEDNTL
CCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCC
PSYLEKNCTKGIELVLKKFDQSKDERDLELEKIKVDTQAKIESLKDDNELKVLLSENDKL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHCCCCCCEEEEECCCCHH
LSSDFKKLTKKLMRSQIINDGKRVDGRELDEVRKISASAGILPKRVHGSALFQRGLTQVL
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
STTTLGTPSDAQEMDDLNPSTEKTYLHHYNFPPFSVGETRPMRTPGRREIGHGALAERAI
HHCCCCCCCCHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHCCCCHHHHCCE
IPVLPGKETFPYVLRVVSEVLSSNGSTSMGSVCGSTLSLLDAGVPLKALVSGTAMGLIKE
EECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
GKEIRILTDIQGIEDFLGDMDFKVAGTEKGITALQMDMKITGLSVSVISDAIKKARPARL
CCEEEEEECHHHHHHHHCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHCCCHHH
HILEKMQEAIDKPQESLSPHAPRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIED
HHHHHHHHHHCCCHHHCCCCCCCEEEEEECHHHHHEEECCCCCEEECHHHCCCCEEEECC
GGIVTIASHDGAAAEEAQKIIEGLTRKVHEGEIFTGVVTRIIPIGAFVEILPGKEGMVHI
CCEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCCCCEEH
SQLSEARVERVEDVVRQGDEVTVRVREIDSRGRINLTLRGVSQNSGMSYPEPTPTPVAPL
HHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA