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The map label for this gene is bioB [H]

Identifier: 78779487

GI number: 78779487

Start: 1017654

End: 1018661

Strand: Direct

Name: bioB [H]

Synonym: PMT9312_1104

Alternate gene names: 78779487

Gene position: 1017654-1018661 (Clockwise)

Preceding gene: 78779486

Following gene: 78779488

Centisome position: 59.54

GC content: 35.12

Gene sequence:

>1008_bases
ATGCCTAATTCGAATAATCAGTTATTAAAAGAAATTAGGTTCGATTGGAATAAAGAGGAGATATTGGAAATACTTGATAT
GCCTCTTATTGATTTAATGTGGGAATCACAAACTGTTCACAGGAAATTTAACAAATACAACATTCAATTAGCATCTTTGT
TCAGCGTAAAAACTGGTGGATGTGAGGAAAATTGTTCGTACTGTAGCCAATCAATTTATAGTGCTAGCGAAATCAAAAGT
CATCCACAATTTCAAGTTGAGGAGGTTATGGCAAGAGCTAAAATAGCAAAAAATGAGGGCGCAGATAGATTTTGTATGGG
TTGGGCGTGGAGAGAAATTAGAGATGGGAAATCTTTTAATGCAATGTTAGAGATGGTTAGCGGTGTAAGAGATTTAGGGA
TGGAAGCATGCGTTACTGCTGGGATGCTTACAGAAGAACAAGCTTCCAGGCTGGCTGATGCAGGTTTGACCGCTTATAAC
CACAATCTTGATACTAGTCCTGAGCATTATAAAAATATTATTACTACTAGAACTTATCAAGATAGACTAGATACTATAAA
AAGAGTAAGGAATGCAGGAATAAATGTTTGTTGTGGAGGGATAATAGGCTTGGGTGAAACTAATGGCGATAGAGCATCCC
TTTTGGAAGTACTTTCAAACATGAATCCACACCCTGAAAGTGTTCCTATAAATTCATTAGTAGCTATTGAAGGTACTGGT
TTAGAAGATAATCAAGAAATTGATTCGATTGAAATGATAAGGATGATAGCTACGGCAAGAATTCTTATGCCTAAAAGTAA
AATAAGATTAAGTGCAGGGCGAGAGAAGCTTTCAAAAGAAGCCCAAATTTTATGTTTTCAATGTGGGGCAAATTCAATTT
TCTATGGAGATGAGTTACTCACAACTTCAAATCCATCATTTCAATCAGACAGAAAACTTCTTAAAGAGGTAGGAGTATCA
TTTAACAAAGATTTTGAAACTCGTGAAAAAACATTATTTTCTTTATGA

Upstream 100 bases:

>100_bases
AGCAATAATTGATTACCAATCAAGAAATAGACGTTTCGGCGGTATAGAATCATTACCAAATGAATCTTTTGAAGATTCTC
AATATTCTATCTAATAAAAA

Downstream 100 bases:

>100_bases
AAGGCAAAAATTATAAAATAGTTTCTCTTTACTCTTTCTTCCCATTTCAAGAAAACTTAATTATTGATCTAAAAAGTAAA
TTATTAGAAATCGGATATGA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MPNSNNQLLKEIRFDWNKEEILEILDMPLIDLMWESQTVHRKFNKYNIQLASLFSVKTGGCEENCSYCSQSIYSASEIKS
HPQFQVEEVMARAKIAKNEGADRFCMGWAWREIRDGKSFNAMLEMVSGVRDLGMEACVTAGMLTEEQASRLADAGLTAYN
HNLDTSPEHYKNIITTRTYQDRLDTIKRVRNAGINVCCGGIIGLGETNGDRASLLEVLSNMNPHPESVPINSLVAIEGTG
LEDNQEIDSIEMIRMIATARILMPKSKIRLSAGREKLSKEAQILCFQCGANSIFYGDELLTTSNPSFQSDRKLLKEVGVS
FNKDFETREKTLFSL

Sequences:

>Translated_335_residues
MPNSNNQLLKEIRFDWNKEEILEILDMPLIDLMWESQTVHRKFNKYNIQLASLFSVKTGGCEENCSYCSQSIYSASEIKS
HPQFQVEEVMARAKIAKNEGADRFCMGWAWREIRDGKSFNAMLEMVSGVRDLGMEACVTAGMLTEEQASRLADAGLTAYN
HNLDTSPEHYKNIITTRTYQDRLDTIKRVRNAGINVCCGGIIGLGETNGDRASLLEVLSNMNPHPESVPINSLVAIEGTG
LEDNQEIDSIEMIRMIATARILMPKSKIRLSAGREKLSKEAQILCFQCGANSIFYGDELLTTSNPSFQSDRKLLKEVGVS
FNKDFETREKTLFSL
>Mature_334_residues
PNSNNQLLKEIRFDWNKEEILEILDMPLIDLMWESQTVHRKFNKYNIQLASLFSVKTGGCEENCSYCSQSIYSASEIKSH
PQFQVEEVMARAKIAKNEGADRFCMGWAWREIRDGKSFNAMLEMVSGVRDLGMEACVTAGMLTEEQASRLADAGLTAYNH
NLDTSPEHYKNIITTRTYQDRLDTIKRVRNAGINVCCGGIIGLGETNGDRASLLEVLSNMNPHPESVPINSLVAIEGTGL
EDNQEIDSIEMIRMIATARILMPKSKIRLSAGREKLSKEAQILCFQCGANSIFYGDELLTTSNPSFQSDRKLLKEVGVSF
NKDFETREKTLFSL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=309, Percent_Identity=51.1326860841424, Blast_Score=339, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6321725, Length=326, Percent_Identity=45.7055214723926, Blast_Score=305, Evalue=6e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 37674; Mature: 37543

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNSNNQLLKEIRFDWNKEEILEILDMPLIDLMWESQTVHRKFNKYNIQLASLFSVKTGG
CCCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHCCHHHHHHHHHHEEEEEHEEEECCCC
CEENCSYCSQSIYSASEIKSHPQFQVEEVMARAKIAKNEGADRFCMGWAWREIRDGKSFN
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHH
AMLEMVSGVRDLGMEACVTAGMLTEEQASRLADAGLTAYNHNLDTSPEHYKNIITTRTYQ
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHCCHHH
DRLDTIKRVRNAGINVCCGGIIGLGETNGDRASLLEVLSNMNPHPESVPINSLVAIEGTG
HHHHHHHHHHHCCCEEEECCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC
LEDNQEIDSIEMIRMIATARILMPKSKIRLSAGREKLSKEAQILCFQCGANSIFYGDELL
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHEEEEECCCCCEEECCEEE
TTSNPSFQSDRKLLKEVGVSFNKDFETREKTLFSL
ECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
PNSNNQLLKEIRFDWNKEEILEILDMPLIDLMWESQTVHRKFNKYNIQLASLFSVKTGG
CCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHCCHHHHHHHHHHEEEEEHEEEECCCC
CEENCSYCSQSIYSASEIKSHPQFQVEEVMARAKIAKNEGADRFCMGWAWREIRDGKSFN
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHH
AMLEMVSGVRDLGMEACVTAGMLTEEQASRLADAGLTAYNHNLDTSPEHYKNIITTRTYQ
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHCCHHH
DRLDTIKRVRNAGINVCCGGIIGLGETNGDRASLLEVLSNMNPHPESVPINSLVAIEGTG
HHHHHHHHHHHCCCEEEECCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC
LEDNQEIDSIEMIRMIATARILMPKSKIRLSAGREKLSKEAQILCFQCGANSIFYGDELL
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHEEEEECCCCCEEECCEEE
TTSNPSFQSDRKLLKEVGVSFNKDFETREKTLFSL
ECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA