Click here to switch to the map view.

The map label for this gene is gldA [C]

Identifier: 78779481

GI number: 78779481

Start: 1010190

End: 1011281

Strand: Reverse

Name: gldA [C]

Synonym: PMT9312_1098

Alternate gene names: 78779481

Gene position: 1011281-1010190 (Counterclockwise)

Preceding gene: 78779482

Following gene: 78779480

Centisome position: 59.17

GC content: 31.23

Gene sequence:

>1092_bases
ATGCAGTTAATTTCTCCAGAAATTGTATTTAGGGGGAAAGATGCTTGGGAAAAAGCTTTACCTCAAATTACTAAATTAAC
CAAAAGTCCATTAATTTTAGGTAGGGGGATTCATACAAATAATCTGAGAAATAAAATATTTAATGATTTAAAAAATCAAA
ACCTTATTGTTAATTCTGCTAATTTACAATTTGATTGTTGTTATGAAGACATTTCAAGAGTAAAGAATATCATTTTAAAG
AATAATAATGATTCTGTTATCGCTGCTGGAGGTGGCAAGGTTCTAGATTCTGGGAAATATATAGCAGAGTGTCTTAATAT
CCCTTGTATTACAGTGCCTCTTAGCGCCTCTACATGTGCAGGTTGGACAGCCTTATCGAATATTTATACAAAAGATGGCC
AATTCATAAAGGATGTTGCATTAGGATCTTGTCCAAGAATCCTAGTATATGATCATAAATTTATTCAAAAAGCCCCATCT
AGAACACTTGCTAGTGGCATAGCTGATGCTTTGGCAAAATGGTATGAATCCTCAATAACAAGTTCAACAATAGATGATGG
ACTTGTTCAACAAGCGATTCAGATATCAAGAGTTTTAAGAGATCAACTATTAATAGATGGAGAAAAAGCTTTTAAAGGAC
AATTCGAAAATAATCCTTCTTGGCGAAATACTGTAGAAGCTTGTGGACTTACAGCAGGATTAATCGGAGGTATTGGTGGA
GAAAAGTGTAGGACTGCCGCAGCACATGCTATTCATAATGCAATTACTCAGATAATTACCCCTAATAAATTCTTACATGG
TGAGATTGTTGGAGTAGGATTATTATTGCAATTAAGACTAGAAGAAATTAAAAATAATAATAAATTAGCTGATCAATCGA
TTAAACAATTATTGGTACTCATGAAAGAATTGAAATTACCAACTACTATTGCACAACTTGGAATAAATGTTTTTGAAAAT
AATAATTTAGAGAAAATTGCTGATTTTACTTGTCGAGATAAATCTGAGATTCATTTTTTGCCTTTTGAAATTTTTAAACA
GGACATAATAGAGGTCATTACAAATTTTGAACAACAAAAAATTAAAATATAA

Upstream 100 bases:

>100_bases
TAGTTGATATAGACGATAATAAAAAAGTTACAATCAATATTTCTTCTGAAGAGTCTTCTCAAGAGTTAGCAAGTGCTAAC
TTTTAGGCATTAAAACAAAT

Downstream 100 bases:

>100_bases
TCATTTTTTTGACTAAAAACCATTTTGAACAATTAAATAATTTAAATGTGGAATTTTTCGAAAGTGCCAAATTGTCACTA
TTAGACCCATTAGGTCTTTA

Product: putative glycerol dehydrogenase

Products: glycerone; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MQLISPEIVFRGKDAWEKALPQITKLTKSPLILGRGIHTNNLRNKIFNDLKNQNLIVNSANLQFDCCYEDISRVKNIILK
NNNDSVIAAGGGKVLDSGKYIAECLNIPCITVPLSASTCAGWTALSNIYTKDGQFIKDVALGSCPRILVYDHKFIQKAPS
RTLASGIADALAKWYESSITSSTIDDGLVQQAIQISRVLRDQLLIDGEKAFKGQFENNPSWRNTVEACGLTAGLIGGIGG
EKCRTAAAHAIHNAITQIITPNKFLHGEIVGVGLLLQLRLEEIKNNNKLADQSIKQLLVLMKELKLPTTIAQLGINVFEN
NNLEKIADFTCRDKSEIHFLPFEIFKQDIIEVITNFEQQKIKI

Sequences:

>Translated_363_residues
MQLISPEIVFRGKDAWEKALPQITKLTKSPLILGRGIHTNNLRNKIFNDLKNQNLIVNSANLQFDCCYEDISRVKNIILK
NNNDSVIAAGGGKVLDSGKYIAECLNIPCITVPLSASTCAGWTALSNIYTKDGQFIKDVALGSCPRILVYDHKFIQKAPS
RTLASGIADALAKWYESSITSSTIDDGLVQQAIQISRVLRDQLLIDGEKAFKGQFENNPSWRNTVEACGLTAGLIGGIGG
EKCRTAAAHAIHNAITQIITPNKFLHGEIVGVGLLLQLRLEEIKNNNKLADQSIKQLLVLMKELKLPTTIAQLGINVFEN
NNLEKIADFTCRDKSEIHFLPFEIFKQDIIEVITNFEQQKIKI
>Mature_363_residues
MQLISPEIVFRGKDAWEKALPQITKLTKSPLILGRGIHTNNLRNKIFNDLKNQNLIVNSANLQFDCCYEDISRVKNIILK
NNNDSVIAAGGGKVLDSGKYIAECLNIPCITVPLSASTCAGWTALSNIYTKDGQFIKDVALGSCPRILVYDHKFIQKAPS
RTLASGIADALAKWYESSITSSTIDDGLVQQAIQISRVLRDQLLIDGEKAFKGQFENNPSWRNTVEACGLTAGLIGGIGG
EKCRTAAAHAIHNAITQIITPNKFLHGEIVGVGLLLQLRLEEIKNNNKLADQSIKQLLVLMKELKLPTTIAQLGINVFEN
NNLEKIADFTCRDKSEIHFLPFEIFKQDIIEVITNFEQQKIKI

Specific function: Glycerol utilization. [C]

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=344, Percent_Identity=30.5232558139535, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1786815, Length=281, Percent_Identity=33.4519572953737, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.6

Molecular weight: Translated: 40169; Mature: 40169

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLISPEIVFRGKDAWEKALPQITKLTKSPLILGRGIHTNNLRNKIFNDLKNQNLIVNSA
CCCCCCCEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCEEEECC
NLQFDCCYEDISRVKNIILKNNNDSVIAAGGGKVLDSGKYIAECLNIPCITVPLSASTCA
CCEEEHHHHHHHHHHHHEEECCCCCEEEECCCCEECCCHHHHHHHCCCEEEEECCCCHHH
GWTALSNIYTKDGQFIKDVALGSCPRILVYDHKFIQKAPSRTLASGIADALAKWYESSIT
HHHHHHHHHCCCCCHHHHHHHCCCCEEEEECHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
SSTIDDGLVQQAIQISRVLRDQLLIDGEKAFKGQFENNPSWRNTVEACGLTAGLIGGIGG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
EKCRTAAAHAIHNAITQIITPNKFLHGEIVGVGLLLQLRLEEIKNNNKLADQSIKQLLVL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MKELKLPTTIAQLGINVFENNNLEKIADFTCRDKSEIHFLPFEIFKQDIIEVITNFEQQK
HHHHCCCHHHHHHCCHHHCCCCHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCHHHH
IKI
CCC
>Mature Secondary Structure
MQLISPEIVFRGKDAWEKALPQITKLTKSPLILGRGIHTNNLRNKIFNDLKNQNLIVNSA
CCCCCCCEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCEEEECC
NLQFDCCYEDISRVKNIILKNNNDSVIAAGGGKVLDSGKYIAECLNIPCITVPLSASTCA
CCEEEHHHHHHHHHHHHEEECCCCCEEEECCCCEECCCHHHHHHHCCCEEEEECCCCHHH
GWTALSNIYTKDGQFIKDVALGSCPRILVYDHKFIQKAPSRTLASGIADALAKWYESSIT
HHHHHHHHHCCCCCHHHHHHHCCCCEEEEECHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
SSTIDDGLVQQAIQISRVLRDQLLIDGEKAFKGQFENNPSWRNTVEACGLTAGLIGGIGG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
EKCRTAAAHAIHNAITQIITPNKFLHGEIVGVGLLLQLRLEEIKNNNKLADQSIKQLLVL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MKELKLPTTIAQLGINVFENNNLEKIADFTCRDKSEIHFLPFEIFKQDIIEVITNFEQQK
HHHHCCCHHHHHHCCHHHCCCCHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCHHHH
IKI
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+; NADH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.13 {NAD+}} 0.12 {NADH}} 0.06 {dihydroxyacetone}} 0.5 {glycerol}} [C]

Substrates: glycerol; NAD+

Specific reaction: glycerol + NAD+ = glycerone + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Chelatingagents; Li+; Na+; Thiolcompounds [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]