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The map label for this gene is folD [H]

Identifier: 78779463

GI number: 78779463

Start: 990982

End: 991878

Strand: Direct

Name: folD [H]

Synonym: PMT9312_1080

Alternate gene names: 78779463

Gene position: 990982-991878 (Clockwise)

Preceding gene: 78779461

Following gene: 78779464

Centisome position: 57.98

GC content: 33.11

Gene sequence:

>897_bases
ATGTCATTAAAATTAGACGGTAAAAAATTATCTCTTGAAATTGAAGAAAGATTAAATGACTATATTTTTATTAATAAAAA
AATCGCAAAAAGGGCTCCTGGTCTAGCCGTAATAAGAATAGGTGAAGACCCAGCGAGTGGTGTTTACGTTAATAACAAGG
AAAAAGCATGTTCAAGGGTTGGGATAAAGAGCTTTATCTTTCATCTAAAAGATAATGTAGAGCAAAAAGAAGTTGAACAA
TTAATAAACAAACTAAACTTTGATAAGAATATTGATGGAATGTTGCTACAACTCCCCATTCCAAAGAAGTTTGATGAGCA
ACGACTGATAAGTTATATCAATCCAAGCAAAGATGTAGATGGATTAAATGAGATAAACATCGGCAAATTAGTGAAAAATG
AGCCTGCGATGAGATCATGTACACCAGCAGGAATTATTAATTTATTAAGGTCCCAAAATATTACAATTGAAGGAAAGAAA
ATTGTTGTTATCGGAAGAAGTTTACTAGTTGGAAAACCTTTATCGCTTATGTTGTTAAACCTTAATGGGACGGTAACAAT
GACTCATTCAAAAACATTAGATTTAAACAAAGTCTGTAAGGAAGCTGACATTCTAATTGCTGCTGCAGGGAAACCAAATC
TTATAGATTCTAGTTTTGTAAAAGAAGGTGCAGTGATTATTGATGTTGGAATACATAGGTTAAAAAGTTCTGATAAAAAT
AAAACCAGATTATGTGGCGATGTATTATTAGAAGATGTCATTCCTAAAGTATTGGCTTACACGCCTGTTCCCGGAGGTGT
TGGACCAATGACCGTAACAATGTTACTGGTAAATACTATTTTTAGCTGGCAAAAACAATTTGGCTTATCGTCAACCCTAA
ATGACCTTTTGCCATAA

Upstream 100 bases:

>100_bases
TTTAGAATAGTTGTAATGTTTTGCACAGAATATAATTAAATCTATTTGAATAATTTAAAATTATAGGCATGCTAGGTGTA
AAAAGGAGATCTTTTTCGAA

Downstream 100 bases:

>100_bases
ATCCTAAGATGAAAAAAATTATTTTAAAAGGTATAAAATGACTGAAGCTATAAGCAACATTTCAGATTTCGAAGAATATC
TTAAAAACGCAAAAAAAGTT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSLKLDGKKLSLEIEERLNDYIFINKKIAKRAPGLAVIRIGEDPASGVYVNNKEKACSRVGIKSFIFHLKDNVEQKEVEQ
LINKLNFDKNIDGMLLQLPIPKKFDEQRLISYINPSKDVDGLNEINIGKLVKNEPAMRSCTPAGIINLLRSQNITIEGKK
IVVIGRSLLVGKPLSLMLLNLNGTVTMTHSKTLDLNKVCKEADILIAAAGKPNLIDSSFVKEGAVIIDVGIHRLKSSDKN
KTRLCGDVLLEDVIPKVLAYTPVPGGVGPMTVTMLLVNTIFSWQKQFGLSSTLNDLLP

Sequences:

>Translated_298_residues
MSLKLDGKKLSLEIEERLNDYIFINKKIAKRAPGLAVIRIGEDPASGVYVNNKEKACSRVGIKSFIFHLKDNVEQKEVEQ
LINKLNFDKNIDGMLLQLPIPKKFDEQRLISYINPSKDVDGLNEINIGKLVKNEPAMRSCTPAGIINLLRSQNITIEGKK
IVVIGRSLLVGKPLSLMLLNLNGTVTMTHSKTLDLNKVCKEADILIAAAGKPNLIDSSFVKEGAVIIDVGIHRLKSSDKN
KTRLCGDVLLEDVIPKVLAYTPVPGGVGPMTVTMLLVNTIFSWQKQFGLSSTLNDLLP
>Mature_297_residues
SLKLDGKKLSLEIEERLNDYIFINKKIAKRAPGLAVIRIGEDPASGVYVNNKEKACSRVGIKSFIFHLKDNVEQKEVEQL
INKLNFDKNIDGMLLQLPIPKKFDEQRLISYINPSKDVDGLNEINIGKLVKNEPAMRSCTPAGIINLLRSQNITIEGKKI
VVIGRSLLVGKPLSLMLLNLNGTVTMTHSKTLDLNKVCKEADILIAAAGKPNLIDSSFVKEGAVIIDVGIHRLKSSDKNK
TRLCGDVLLEDVIPKVLAYTPVPGGVGPMTVTMLLVNTIFSWQKQFGLSSTLNDLLP

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=41.8918918918919, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI222418558, Length=292, Percent_Identity=39.7260273972603, Blast_Score=193, Evalue=1e-49,
Organism=Homo sapiens, GI222136639, Length=287, Percent_Identity=37.2822299651568, Blast_Score=192, Evalue=2e-49,
Organism=Homo sapiens, GI36796743, Length=199, Percent_Identity=27.6381909547739, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786741, Length=276, Percent_Identity=42.7536231884058, Blast_Score=231, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI17568735, Length=268, Percent_Identity=39.1791044776119, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6321643, Length=299, Percent_Identity=40.133779264214, Blast_Score=212, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6319558, Length=298, Percent_Identity=40.2684563758389, Blast_Score=210, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6322933, Length=294, Percent_Identity=26.1904761904762, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI17136818, Length=297, Percent_Identity=39.7306397306397, Blast_Score=207, Evalue=6e-54,
Organism=Drosophila melanogaster, GI17136816, Length=297, Percent_Identity=39.7306397306397, Blast_Score=207, Evalue=9e-54,
Organism=Drosophila melanogaster, GI62472483, Length=283, Percent_Identity=36.3957597173145, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI45551871, Length=283, Percent_Identity=36.3957597173145, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24645718, Length=283, Percent_Identity=36.3957597173145, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI17137370, Length=283, Percent_Identity=36.3957597173145, Blast_Score=185, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 32823; Mature: 32692

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKLDGKKLSLEIEERLNDYIFINKKIAKRAPGLAVIRIGEDPASGVYVNNKEKACSRV
CCEECCCCEEEEEHHHCCCCEEEEEHHHHHCCCCEEEEEECCCCCCCEEECCHHHHHHHH
GIKSFIFHLKDNVEQKEVEQLINKLNFDKNIDGMLLQLPIPKKFDEQRLISYINPSKDVD
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCC
GLNEINIGKLVKNEPAMRSCTPAGIINLLRSQNITIEGKKIVVIGRSLLVGKPLSLMLLN
CCCCCCHHHHHCCCCCHHCCCHHHHHHHHHCCCEEECCCEEEEEECHHHHCCCCEEEEEE
LNGTVTMTHSKTLDLNKVCKEADILIAAAGKPNLIDSSFVKEGAVIIDVGIHRLKSSDKN
CCCEEEEEECCCCCHHHHHCCCCEEEEECCCCCCCCHHHHHCCCEEEEECHHHHHCCCCC
KTRLCGDVLLEDVIPKVLAYTPVPGGVGPMTVTMLLVNTIFSWQKQFGLSSTLNDLLP
HHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure 
SLKLDGKKLSLEIEERLNDYIFINKKIAKRAPGLAVIRIGEDPASGVYVNNKEKACSRV
CEECCCCEEEEEHHHCCCCEEEEEHHHHHCCCCEEEEEECCCCCCCEEECCHHHHHHHH
GIKSFIFHLKDNVEQKEVEQLINKLNFDKNIDGMLLQLPIPKKFDEQRLISYINPSKDVD
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCC
GLNEINIGKLVKNEPAMRSCTPAGIINLLRSQNITIEGKKIVVIGRSLLVGKPLSLMLLN
CCCCCCHHHHHCCCCCHHCCCHHHHHHHHHCCCEEECCCEEEEEECHHHHCCCCEEEEEE
LNGTVTMTHSKTLDLNKVCKEADILIAAAGKPNLIDSSFVKEGAVIIDVGIHRLKSSDKN
CCCEEEEEECCCCCHHHHHCCCCEEEEECCCCCCCCHHHHHCCCEEEEECHHHHHCCCCC
KTRLCGDVLLEDVIPKVLAYTPVPGGVGPMTVTMLLVNTIFSWQKQFGLSSTLNDLLP
HHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA