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The map label for this gene is gsiB [H]

Identifier: 78779443

GI number: 78779443

Start: 971275

End: 972846

Strand: Reverse

Name: gsiB [H]

Synonym: PMT9312_1060

Alternate gene names: 78779443

Gene position: 972846-971275 (Counterclockwise)

Preceding gene: 78779444

Following gene: 78779442

Centisome position: 56.92

GC content: 28.63

Gene sequence:

>1572_bases
ATGAAAAAAAAAGTTCTTTTAACAGTATTTTTTATTTTAATTTCTTTTTTACAGAACTCTTGTGGTTCAAAAAAAATATC
CCAAAAAATCATAGTAGCGAGTTCTGGGAAAATCGAATCTTTAGATCCAGCTAGAGCAAATACTCTCAAAGCAATTCAAT
TAATCAGTTCCCTGGGAGACACATTATATGAATTAAATTCTGACGGAGAATTAATACCTGAATTAGCAGCAGGGATGCCA
ATTATTTCAAAGGATAGACTTCAAATAATAATCAATCTAAGAAGAAATGTTTTTTTTCATGATGGAACTTCATTTAACTC
AAATGCAATAAAGTTTACTTTTGATAGATTTAAAAGAATTGGAACAATTAACTATATTTTAGGAAATAAGATTAAATCTA
TAGAAACTCCAAGCGAATATTCAGTTAAAATAAATTTAAATAAACCATCAAGTTCTTTAAATGGTTTACTTACATCAGTA
AATTTAACACCAATATCTCCAACATTTTATAAAGAATATTCTGATAAGTTTCTAAATGAAAAATTCGTTGGTACTGGTAA
GTATGTCTTGACCAGTTTTTCTAATGAAGTTCAATTAATTGATCCAAATTTGAATTATTGGGGTGAAAAGCCCTTAAATA
ATGGTATTAATTTTGTGGGATACTCTAATTCATCTTCTCTTTTTGGAGCTTTAAAAAGTAAACAAATTGACGTACTCTTA
TCAAATTCAATTGATGATAGTCAGAGAAATAGTTTAAGTAATCTAAGCAAAAATAAAAAGTTTAAAGAAGGTAATAGTCC
TTTCACCGAATTAAGTTTTATAAGCCTTAGAACTAGTTCTTATCCATTAAATAATCTTAATTTAAGAATGGCTTTAGCAA
AAAGTCTTAATAGAAAATTAATTAGTGAAAAAGTAAGTTATGGTTTGAGAAAGCCATCAAGATCAATAATTCCTCCGATA
TTAAAAAATGATAATCAAGGTCTGTGGCCTAAATATAATTATTTAGAAGCGAGAAAGTTATTGCAAAAAGAAACTTATTG
CAATGGAAATATACTGAAAATTCCACTTACATATAGAACGAATGTACCAGCAGATAAACTTATTGCCCTCACTTGGCAAG
AAGAAATAAAAAATGTTTTGAATGATTGCATTGAGATAGAACTTAATGGCGTTGAATCTACAACCGTTTATAAAAATCTA
AGTTTGGGAATTTATACAGCAGTTATTCTTGATTGGACTGGTGCTTATTCCGATCCAGAAGCTTATCTCACTCCACTTTT
AAGTTGTAATGAAATAGTTGATGGCATATGTAAAAAAGGTGAATCAGTTTATAGTGGTAGTTTTTGGGGATCTAATAAAG
TGGAAAGTTTATTCCTAAAGAGTGAAAATATAAGTGGTAATAAAAGACTCGACAAACTACTGGAAATTGAAAAAATAGCA
GCAAATTCAATCCCTTACATTCCTATTTGGATATCCTCTCAAAAAGCATGGTCTCAAAATAAAATATCAAATCCTATTTT
TAATGGTGCAGGGATTATTAGATTAAGCGATCTTGAGTTAATTCATGAGTAG

Upstream 100 bases:

>100_bases
CTTGTGATTCAGAAAAAACATTTGCTCTAGAAATAAGTCAAATAACCTTACAGATTTTTTGTTCTAATAAATCCTCAGAA
TAAGTTAGTATTTAAGAAAT

Downstream 100 bases:

>100_bases
GAATTTAAATAAACTTTTTAATTATTCTTTATTAAAAATTGCATTAATACCATTAATGTTATGGATAATTTCTTCATTAG
TTTTTATTTTATTGCGAGTT

Product: ABC transporter substrate-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MKKKVLLTVFFILISFLQNSCGSKKISQKIIVASSGKIESLDPARANTLKAIQLISSLGDTLYELNSDGELIPELAAGMP
IISKDRLQIIINLRRNVFFHDGTSFNSNAIKFTFDRFKRIGTINYILGNKIKSIETPSEYSVKINLNKPSSSLNGLLTSV
NLTPISPTFYKEYSDKFLNEKFVGTGKYVLTSFSNEVQLIDPNLNYWGEKPLNNGINFVGYSNSSSLFGALKSKQIDVLL
SNSIDDSQRNSLSNLSKNKKFKEGNSPFTELSFISLRTSSYPLNNLNLRMALAKSLNRKLISEKVSYGLRKPSRSIIPPI
LKNDNQGLWPKYNYLEARKLLQKETYCNGNILKIPLTYRTNVPADKLIALTWQEEIKNVLNDCIEIELNGVESTTVYKNL
SLGIYTAVILDWTGAYSDPEAYLTPLLSCNEIVDGICKKGESVYSGSFWGSNKVESLFLKSENISGNKRLDKLLEIEKIA
ANSIPYIPIWISSQKAWSQNKISNPIFNGAGIIRLSDLELIHE

Sequences:

>Translated_523_residues
MKKKVLLTVFFILISFLQNSCGSKKISQKIIVASSGKIESLDPARANTLKAIQLISSLGDTLYELNSDGELIPELAAGMP
IISKDRLQIIINLRRNVFFHDGTSFNSNAIKFTFDRFKRIGTINYILGNKIKSIETPSEYSVKINLNKPSSSLNGLLTSV
NLTPISPTFYKEYSDKFLNEKFVGTGKYVLTSFSNEVQLIDPNLNYWGEKPLNNGINFVGYSNSSSLFGALKSKQIDVLL
SNSIDDSQRNSLSNLSKNKKFKEGNSPFTELSFISLRTSSYPLNNLNLRMALAKSLNRKLISEKVSYGLRKPSRSIIPPI
LKNDNQGLWPKYNYLEARKLLQKETYCNGNILKIPLTYRTNVPADKLIALTWQEEIKNVLNDCIEIELNGVESTTVYKNL
SLGIYTAVILDWTGAYSDPEAYLTPLLSCNEIVDGICKKGESVYSGSFWGSNKVESLFLKSENISGNKRLDKLLEIEKIA
ANSIPYIPIWISSQKAWSQNKISNPIFNGAGIIRLSDLELIHE
>Mature_523_residues
MKKKVLLTVFFILISFLQNSCGSKKISQKIIVASSGKIESLDPARANTLKAIQLISSLGDTLYELNSDGELIPELAAGMP
IISKDRLQIIINLRRNVFFHDGTSFNSNAIKFTFDRFKRIGTINYILGNKIKSIETPSEYSVKINLNKPSSSLNGLLTSV
NLTPISPTFYKEYSDKFLNEKFVGTGKYVLTSFSNEVQLIDPNLNYWGEKPLNNGINFVGYSNSSSLFGALKSKQIDVLL
SNSIDDSQRNSLSNLSKNKKFKEGNSPFTELSFISLRTSSYPLNNLNLRMALAKSLNRKLISEKVSYGLRKPSRSIIPPI
LKNDNQGLWPKYNYLEARKLLQKETYCNGNILKIPLTYRTNVPADKLIALTWQEEIKNVLNDCIEIELNGVESTTVYKNL
SLGIYTAVILDWTGAYSDPEAYLTPLLSCNEIVDGICKKGESVYSGSFWGSNKVESLFLKSENISGNKRLDKLLEIEKIA
ANSIPYIPIWISSQKAWSQNKISNPIFNGAGIIRLSDLELIHE

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=389, Percent_Identity=25.4498714652956, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787052, Length=496, Percent_Identity=22.7822580645161, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1787762, Length=354, Percent_Identity=24.8587570621469, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI87081878, Length=472, Percent_Identity=23.0932203389831, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787551, Length=491, Percent_Identity=21.9959266802444, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787495, Length=435, Percent_Identity=20.9195402298851, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58601; Mature: 58601

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKVLLTVFFILISFLQNSCGSKKISQKIIVASSGKIESLDPARANTLKAIQLISSLGD
CCCHHHHHHHHHHHHHHHCCCCCCHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
TLYELNSDGELIPELAAGMPIISKDRLQIIINLRRNVFFHDGTSFNSNAIKFTFDRFKRI
HHEEECCCCCCHHHHHCCCCEECCCHHEEEEEECCCEEEECCCCCCCCCEEEEHHHHHHC
GTINYILGNKIKSIETPSEYSVKINLNKPSSSLNGLLTSVNLTPISPTFYKEYSDKFLNE
CEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCC
KFVGTGKYVLTSFSNEVQLIDPNLNYWGEKPLNNGINFVGYSNSSSLFGALKSKQIDVLL
CCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCEEEEE
SNSIDDSQRNSLSNLSKNKKFKEGNSPFTELSFISLRTSSYPLNNLNLRMALAKSLNRKL
ECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEHHHHHHHHHHHH
ISEKVSYGLRKPSRSIIPPILKNDNQGLWPKYNYLEARKLLQKETYCNGNILKIPLTYRT
HHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEC
NVPADKLIALTWQEEIKNVLNDCIEIELNGVESTTVYKNLSLGIYTAVILDWTGAYSDPE
CCCHHHEEEEEHHHHHHHHHHHHEEEEECCCCCEEEEECCCHHHEEEEEEECCCCCCCCH
AYLTPLLSCNEIVDGICKKGESVYSGSFWGSNKVESLFLKSENISGNKRLDKLLEIEKIA
HHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
ANSIPYIPIWISSQKAWSQNKISNPIFNGAGIIRLSDLELIHE
HCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCHHHCC
>Mature Secondary Structure
MKKKVLLTVFFILISFLQNSCGSKKISQKIIVASSGKIESLDPARANTLKAIQLISSLGD
CCCHHHHHHHHHHHHHHHCCCCCCHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
TLYELNSDGELIPELAAGMPIISKDRLQIIINLRRNVFFHDGTSFNSNAIKFTFDRFKRI
HHEEECCCCCCHHHHHCCCCEECCCHHEEEEEECCCEEEECCCCCCCCCEEEEHHHHHHC
GTINYILGNKIKSIETPSEYSVKINLNKPSSSLNGLLTSVNLTPISPTFYKEYSDKFLNE
CEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCC
KFVGTGKYVLTSFSNEVQLIDPNLNYWGEKPLNNGINFVGYSNSSSLFGALKSKQIDVLL
CCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCEEEEE
SNSIDDSQRNSLSNLSKNKKFKEGNSPFTELSFISLRTSSYPLNNLNLRMALAKSLNRKL
ECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEHHHHHHHHHHHH
ISEKVSYGLRKPSRSIIPPILKNDNQGLWPKYNYLEARKLLQKETYCNGNILKIPLTYRT
HHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEC
NVPADKLIALTWQEEIKNVLNDCIEIELNGVESTTVYKNLSLGIYTAVILDWTGAYSDPE
CCCHHHEEEEEHHHHHHHHHHHHEEEEECCCCCEEEEECCCHHHEEEEEEECCCCCCCCH
AYLTPLLSCNEIVDGICKKGESVYSGSFWGSNKVESLFLKSENISGNKRLDKLLEIEKIA
HHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
ANSIPYIPIWISSQKAWSQNKISNPIFNGAGIIRLSDLELIHE
HCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA