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The map label for this gene is selU [H]

Identifier: 78779259

GI number: 78779259

Start: 808021

End: 809064

Strand: Reverse

Name: selU [H]

Synonym: PMT9312_0875

Alternate gene names: 78779259

Gene position: 809064-808021 (Counterclockwise)

Preceding gene: 78779262

Following gene: 78779258

Centisome position: 47.34

GC content: 27.01

Gene sequence:

>1044_bases
ATGTATTTCAAAAGAAAAGAACTAGAGAAATTTAGAAGTTTTAAAGGACCTCTTATAGATGTTAGGAGCCCGAGTGAATA
TTATAAAGGACATATGCCTAATTCTATTAACATTCCATTATTTAATAATGATGAGAGATCAATTATTGGTACAATTTATA
AAAAAGAGGGTAGAAAAAAAGCAGTAATAGAGGGATTAAAATTTTTTGAAAAAAAAATGGAATTTCTTCTTGATAATTTA
TTTTTGAATATTGATTCATATAAAACTATTCCTGATATCAATAATAATGAATTATTTATCAGAATATATTGCTCTAGAGG
AGGAATGCGGTCACAAAGTATTTCTTGGTTACTAGAAAAATATAAATTAAATCCAATAACACTTAAGGGAGGATACAAAA
CATACAGGAAATGGATATTAGATTGTTTCTCAAAAAAGTGGAATATTATAATTATTGGTGGGAAAACAGGTTCTGGGAAA
ACAAGGTTATTGTCATTACTAGAGAAATATAAATATCAAACTATAGATCTTGAAGGATTTGCTTGTCATAGAGGAAGCAC
ATTTGGTGGTTTAGGAATGAGAGAACAACCTACAAACGAACAATTTGAAAATAAAATAGCAGAAAAATTATATTCCTTTC
AAACTATTAATAATATTTTTGTAGAAGCAGAAAGTGCGAATATTGGTAAATGCAAAATACCTCATGAATTCTTTAATCAG
ATGAAAAAATCAAGGAGGATTGAGATATTAAGGAGCGAATCAAACAGATTAGATGAGTTAATTAATACTTATAGTGTATT
TAAAAAAGAGGAACTCAAGGACTCTGTACAAAGGATTAAAAAAAGATTAGGTCCTCAAAGAACAAAAATAGCTCTTGAAT
CAATAGATGAGGAGAAATGGGATTTAGTATGTAGATCAGTTTTAGATTATTACGATAGGTGTTATGAATATGAAAAGGTT
GGCAAAGAAAATATAACGTTATTAGATTTAACTGATAAGAATTATGATGAAAAAATCATAGAGTTATTAAATAATATTTT
ATAA

Upstream 100 bases:

>100_bases
ATCTACAAATGTTTTAATAAAATCTAATGTAGCATTACTATAGTTATCTTGTTCTTTGTTAGTCATTTTAAATAAATTAA
CCTAAAAATAAAAACTATCC

Downstream 100 bases:

>100_bases
AATATTTGCAAACGAAAGTAAAAAATATTTCTTAAAATAAAAATTATTAATGAAAACTTATGACAGCAAATAAAACTGCA
AAAATACAATTTTATGAAGG

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MYFKRKELEKFRSFKGPLIDVRSPSEYYKGHMPNSINIPLFNNDERSIIGTIYKKEGRKKAVIEGLKFFEKKMEFLLDNL
FLNIDSYKTIPDINNNELFIRIYCSRGGMRSQSISWLLEKYKLNPITLKGGYKTYRKWILDCFSKKWNIIIIGGKTGSGK
TRLLSLLEKYKYQTIDLEGFACHRGSTFGGLGMREQPTNEQFENKIAEKLYSFQTINNIFVEAESANIGKCKIPHEFFNQ
MKKSRRIEILRSESNRLDELINTYSVFKKEELKDSVQRIKKRLGPQRTKIALESIDEEKWDLVCRSVLDYYDRCYEYEKV
GKENITLLDLTDKNYDEKIIELLNNIL

Sequences:

>Translated_347_residues
MYFKRKELEKFRSFKGPLIDVRSPSEYYKGHMPNSINIPLFNNDERSIIGTIYKKEGRKKAVIEGLKFFEKKMEFLLDNL
FLNIDSYKTIPDINNNELFIRIYCSRGGMRSQSISWLLEKYKLNPITLKGGYKTYRKWILDCFSKKWNIIIIGGKTGSGK
TRLLSLLEKYKYQTIDLEGFACHRGSTFGGLGMREQPTNEQFENKIAEKLYSFQTINNIFVEAESANIGKCKIPHEFFNQ
MKKSRRIEILRSESNRLDELINTYSVFKKEELKDSVQRIKKRLGPQRTKIALESIDEEKWDLVCRSVLDYYDRCYEYEKV
GKENITLLDLTDKNYDEKIIELLNNIL
>Mature_347_residues
MYFKRKELEKFRSFKGPLIDVRSPSEYYKGHMPNSINIPLFNNDERSIIGTIYKKEGRKKAVIEGLKFFEKKMEFLLDNL
FLNIDSYKTIPDINNNELFIRIYCSRGGMRSQSISWLLEKYKLNPITLKGGYKTYRKWILDCFSKKWNIIIIGGKTGSGK
TRLLSLLEKYKYQTIDLEGFACHRGSTFGGLGMREQPTNEQFENKIAEKLYSFQTINNIFVEAESANIGKCKIPHEFFNQ
MKKSRRIEILRSESNRLDELINTYSVFKKEELKDSVQRIKKRLGPQRTKIALESIDEEKWDLVCRSVLDYYDRCYEYEKV
GKENITLLDLTDKNYDEKIIELLNNIL

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=338, Percent_Identity=29.8816568047337, Blast_Score=126, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 40956; Mature: 40956

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFKRKELEKFRSFKGPLIDVRSPSEYYKGHMPNSINIPLFNNDERSIIGTIYKKEGRKK
CCCCHHHHHHHHHCCCCEEECCCCHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHCCCHH
AVIEGLKFFEKKMEFLLDNLFLNIDSYKTIPDINNNELFIRIYCSRGGMRSQSISWLLEK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
YKLNPITLKGGYKTYRKWILDCFSKKWNIIIIGGKTGSGKTRLLSLLEKYKYQTIDLEGF
HCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCEEECCCCE
ACHRGSTFGGLGMREQPTNEQFENKIAEKLYSFQTINNIFVEAESANIGKCKIPHEFFNQ
EECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEECCHHHHHH
MKKSRRIEILRSESNRLDELINTYSVFKKEELKDSVQRIKKRLGPQRTKIALESIDEEKW
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHH
DLVCRSVLDYYDRCYEYEKVGKENITLLDLTDKNYDEKIIELLNNIL
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MYFKRKELEKFRSFKGPLIDVRSPSEYYKGHMPNSINIPLFNNDERSIIGTIYKKEGRKK
CCCCHHHHHHHHHCCCCEEECCCCHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHCCCHH
AVIEGLKFFEKKMEFLLDNLFLNIDSYKTIPDINNNELFIRIYCSRGGMRSQSISWLLEK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
YKLNPITLKGGYKTYRKWILDCFSKKWNIIIIGGKTGSGKTRLLSLLEKYKYQTIDLEGF
HCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCEEECCCCE
ACHRGSTFGGLGMREQPTNEQFENKIAEKLYSFQTINNIFVEAESANIGKCKIPHEFFNQ
EECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEECCHHHHHH
MKKSRRIEILRSESNRLDELINTYSVFKKEELKDSVQRIKKRLGPQRTKIALESIDEEKW
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHH
DLVCRSVLDYYDRCYEYEKVGKENITLLDLTDKNYDEKIIELLNNIL
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA