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The map label for this gene is pdhB [H]

Identifier: 78779253

GI number: 78779253

Start: 802953

End: 803936

Strand: Direct

Name: pdhB [H]

Synonym: PMT9312_0869

Alternate gene names: 78779253

Gene position: 802953-803936 (Clockwise)

Preceding gene: 78779252

Following gene: 78779254

Centisome position: 46.98

GC content: 34.15

Gene sequence:

>984_bases
GTGGCTGGAACATTATTATTTAATGCTTTGAAAGAGGCAATTGATGAAGAAATGGCAAATGATGTAAATGTTTGCGTTAT
GGGGGAAGATGTTGGTCAATATGGAGGATCTTATAAGGTAACTAAAGATTTATATGAAAAATATGGAGAGTTAAGAGTCT
TAGATACTCCAATTGCAGAGAATAGTTTTACAGGCATGGCTGTGGGTGCAGCAATGACTGGCTTAAGACCAATAGTAGAA
GGAATGAATATGGGTTTTTTGCTTTTAGCTTTTAATCAGATATCAAATAATATGGGTATGCTTAGATATACTAGTGGCGG
AAATTATAAAATACCAGCAGTAGTTCGAGGACCTGGAGGAGTTGGTCGTCAACTTGGTGCTGAGCACAGTCAAAGACTTG
AAGCATATTTTCATGCAGTTCCTGGCATAAAGATTGTGGCATGTAGTACACCCACAAATGCTAAAGGTTTAATGAAAGCA
GCTATAAGAGATGATAATCCAGTTCTATTTTTCGAACATGTTCTTCTATACAATTTGTCTGAAGAATTACCTGAGGGTGA
TTATACTTGCGCTTTAGATCAGGCTGACGTTGTAAAAGAAGGGAAAGATATTACTTTATTGACTTATTCAAGAATGAGAC
ATCACTGTCTTAAAGCTGTTGAAGAATTAGAAAAAAAAGGAATAGATGTTGAGTTAATAGATTTAATAAGTTTAAAACCA
TTTGATATCGAAACCATCTCAAAATCAATAAGAAAAACAAATAAAGTAATTATTGTTGAAGAATGTATGAAGACTGGAGG
TATTGGTGCAGAATTAATTGCCTTGATAACAGAAGAGTGTTTTGATGATCTTGATGCCCGACCAATTAGATTATCTAGTC
AGGATATTCCAACTCCTTATAATGGAAATCTTGAGAATTTGACAATAATCCAACCACATCAAATAGTTGAAAAAGTTGAA
CAGTTAATTAGTGGGAGTATATAG

Upstream 100 bases:

>100_bases
GGAATGATTTTATCAATATTACTACTCATAACCTTCGCTGAAGGAGGTTGAATTATTCAAAGTGGTAGGATTATATGTGT
GATTAATTAGGTAATTAATT

Downstream 100 bases:

>100_bases
ACAATGAAAAGAAGGCAAGGTTGGCTTTTTTTTATTTTATTTCTACTTACTTTATCTGTTTATCTATTAATAAATTATCC
CTTACAGTTGGGATTGGATT

Product: pyruvate dehydrogenase E1 beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MAGTLLFNALKEAIDEEMANDVNVCVMGEDVGQYGGSYKVTKDLYEKYGELRVLDTPIAENSFTGMAVGAAMTGLRPIVE
GMNMGFLLLAFNQISNNMGMLRYTSGGNYKIPAVVRGPGGVGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKA
AIRDDNPVLFFEHVLLYNLSEELPEGDYTCALDQADVVKEGKDITLLTYSRMRHHCLKAVEELEKKGIDVELIDLISLKP
FDIETISKSIRKTNKVIIVEECMKTGGIGAELIALITEECFDDLDARPIRLSSQDIPTPYNGNLENLTIIQPHQIVEKVE
QLISGSI

Sequences:

>Translated_327_residues
MAGTLLFNALKEAIDEEMANDVNVCVMGEDVGQYGGSYKVTKDLYEKYGELRVLDTPIAENSFTGMAVGAAMTGLRPIVE
GMNMGFLLLAFNQISNNMGMLRYTSGGNYKIPAVVRGPGGVGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKA
AIRDDNPVLFFEHVLLYNLSEELPEGDYTCALDQADVVKEGKDITLLTYSRMRHHCLKAVEELEKKGIDVELIDLISLKP
FDIETISKSIRKTNKVIIVEECMKTGGIGAELIALITEECFDDLDARPIRLSSQDIPTPYNGNLENLTIIQPHQIVEKVE
QLISGSI
>Mature_326_residues
AGTLLFNALKEAIDEEMANDVNVCVMGEDVGQYGGSYKVTKDLYEKYGELRVLDTPIAENSFTGMAVGAAMTGLRPIVEG
MNMGFLLLAFNQISNNMGMLRYTSGGNYKIPAVVRGPGGVGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKAA
IRDDNPVLFFEHVLLYNLSEELPEGDYTCALDQADVVKEGKDITLLTYSRMRHHCLKAVEELEKKGIDVELIDLISLKPF
DIETISKSIRKTNKVIIVEECMKTGGIGAELIALITEECFDDLDARPIRLSSQDIPTPYNGNLENLTIIQPHQIVEKVEQ
LISGSI

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=322, Percent_Identity=39.7515527950311, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI291084858, Length=322, Percent_Identity=37.5776397515528, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI4557353, Length=317, Percent_Identity=36.5930599369085, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI34101272, Length=317, Percent_Identity=36.5930599369085, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17538422, Length=319, Percent_Identity=41.3793103448276, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17506935, Length=298, Percent_Identity=35.9060402684564, Blast_Score=179, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6319698, Length=322, Percent_Identity=42.8571428571429, Blast_Score=268, Evalue=9e-73,
Organism=Drosophila melanogaster, GI21358145, Length=325, Percent_Identity=40, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24650940, Length=325, Percent_Identity=40, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI160714832, Length=323, Percent_Identity=36.8421052631579, Blast_Score=204, Evalue=5e-53,
Organism=Drosophila melanogaster, GI160714828, Length=323, Percent_Identity=36.8421052631579, Blast_Score=204, Evalue=6e-53,
Organism=Drosophila melanogaster, GI24650943, Length=86, Percent_Identity=51.1627906976744, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24650945, Length=86, Percent_Identity=51.1627906976744, Blast_Score=101, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 35889; Mature: 35758

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTLLFNALKEAIDEEMANDVNVCVMGEDVGQYGGSYKVTKDLYEKYGELRVLDTPIAE
CCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCEEEHHHHHHHHCCEEEEECCCCC
NSFTGMAVGAAMTGLRPIVEGMNMGFLLLAFNQISNNMGMLRYTSGGNYKIPAVVRGPGG
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCEEEEECCCCEEEEEEEECCCC
VGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKAAIRDDNPVLFFEHVLLYNLS
CCHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHH
EELPEGDYTCALDQADVVKEGKDITLLTYSRMRHHCLKAVEELEKKGIDVELIDLISLKP
HHCCCCCCEEEECCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
FDIETISKSIRKTNKVIIVEECMKTGGIGAELIALITEECFDDLDARPIRLSSQDIPTPY
CCHHHHHHHHHHHCCEEEEHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
NGNLENLTIIQPHQIVEKVEQLISGSI
CCCCCEEEEECHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AGTLLFNALKEAIDEEMANDVNVCVMGEDVGQYGGSYKVTKDLYEKYGELRVLDTPIAE
CHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCEEEHHHHHHHHCCEEEEECCCCC
NSFTGMAVGAAMTGLRPIVEGMNMGFLLLAFNQISNNMGMLRYTSGGNYKIPAVVRGPGG
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCEEEEECCCCEEEEEEEECCCC
VGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKAAIRDDNPVLFFEHVLLYNLS
CCHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHH
EELPEGDYTCALDQADVVKEGKDITLLTYSRMRHHCLKAVEELEKKGIDVELIDLISLKP
HHCCCCCCEEEECCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
FDIETISKSIRKTNKVIIVEECMKTGGIGAELIALITEECFDDLDARPIRLSSQDIPTPY
CCHHHHHHHHHHHCCEEEEHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
NGNLENLTIIQPHQIVEKVEQLISGSI
CCCCCEEEEECHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]