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The map label for this gene is lipB [H]

Identifier: 78778782

GI number: 78778782

Start: 376248

End: 376898

Strand: Direct

Name: lipB [H]

Synonym: PMT9312_0397

Alternate gene names: 78778782

Gene position: 376248-376898 (Clockwise)

Preceding gene: 78778777

Following gene: 78778783

Centisome position: 22.01

GC content: 25.5

Gene sequence:

>651_bases
ATGGTTAATAGAACAGCAATAATTAAACAACCTGATAATATTTCTTGTTTCAATGATGTTTATAAATTGCAAAAAGAATA
TCAGGAGGCATTAATTTTAGATAACTCAAACCCTGATTTTATTTGGATAGGTGAGCACCAACTCTGTTATACCTTGGGTA
GGGGATCTAATTACGATAATTTACTATTTTCGTTGAATGATGAAAAATATGATGTTTTTAAAATTGATAGAGGCGGTGAG
GTTACTTGTCATATGCCTGGCCAATTAGTAACTTACTTAATTTTAGATTTGAAAAATTTTAATAAAGATTTAAATTGGTA
TTTAAGAAAAATTGAAAAAATCATTATAAAAATTCTTGGAAATTTTAATATAGATTGTCACTTAAAAAAGGGTTTTACCG
GTGTTTGGATAGAAAATAAGAAAATTGCTTCAATTGGAATTGGGTGTAGAAGATGGATTACGATAAATGGATTTTCAATT
AATTTTAACTGCGAATTAGAAAATTTTAATAAAATTGTTCCTTGCGGAATTGAGAATTGTTTAATGGCAAATATGATAGA
TTACAATAAAAATTTAAATATTAAAGAAGTCAAGAAAATTGTTAAAAAAATCATTCAGGAAGAATTTAATTTTGATTTTG
TATCAAAATAG

Upstream 100 bases:

>100_bases
TATTCTTTTAATGCTCCAGTGAGCTCAAGATTCTTTCCATGGATTAAAATTTTCATAACAAATCTAAAAATATTTACTTA
CTTTCTAAATCTACTTAAAT

Downstream 100 bases:

>100_bases
AAATCAAAATTCCTAAATAAATTTAATATGGGTAATTTTGCGTATTGGCCTTCAGCTAAATCTTTTTATGAAAAAGATAA
ATTTGCTAAAAATAGAGATT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MVNRTAIIKQPDNISCFNDVYKLQKEYQEALILDNSNPDFIWIGEHQLCYTLGRGSNYDNLLFSLNDEKYDVFKIDRGGE
VTCHMPGQLVTYLILDLKNFNKDLNWYLRKIEKIIIKILGNFNIDCHLKKGFTGVWIENKKIASIGIGCRRWITINGFSI
NFNCELENFNKIVPCGIENCLMANMIDYNKNLNIKEVKKIVKKIIQEEFNFDFVSK

Sequences:

>Translated_216_residues
MVNRTAIIKQPDNISCFNDVYKLQKEYQEALILDNSNPDFIWIGEHQLCYTLGRGSNYDNLLFSLNDEKYDVFKIDRGGE
VTCHMPGQLVTYLILDLKNFNKDLNWYLRKIEKIIIKILGNFNIDCHLKKGFTGVWIENKKIASIGIGCRRWITINGFSI
NFNCELENFNKIVPCGIENCLMANMIDYNKNLNIKEVKKIVKKIIQEEFNFDFVSK
>Mature_216_residues
MVNRTAIIKQPDNISCFNDVYKLQKEYQEALILDNSNPDFIWIGEHQLCYTLGRGSNYDNLLFSLNDEKYDVFKIDRGGE
VTCHMPGQLVTYLILDLKNFNKDLNWYLRKIEKIIIKILGNFNIDCHLKKGFTGVWIENKKIASIGIGCRRWITINGFSI
NFNCELENFNKIVPCGIENCLMANMIDYNKNLNIKEVKKIVKKIIQEEFNFDFVSK

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=171, Percent_Identity=27.4853801169591, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI87081767, Length=181, Percent_Identity=29.8342541436464, Blast_Score=100, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323268, Length=139, Percent_Identity=38.1294964028777, Blast_Score=89, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21355483, Length=173, Percent_Identity=32.9479768786127, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 25237; Mature: 25237

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNRTAIIKQPDNISCFNDVYKLQKEYQEALILDNSNPDFIWIGEHQLCYTLGRGSNYDN
CCCCEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCEEEECCCEEEEEECCCCCCCC
LLFSLNDEKYDVFKIDRGGEVTCHMPGQLVTYLILDLKNFNKDLNWYLRKIEKIIIKILG
EEEEECCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
NFNIDCHLKKGFTGVWIENKKIASIGIGCRRWITINGFSINFNCELENFNKIVPCGIENC
CCCEEEEEECCCEEEEEECCEEEEECCCEEEEEEEECEEEEEEEEECCCCEEECCCCHHH
LMANMIDYNKNLNIKEVKKIVKKIIQEEFNFDFVSK
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVNRTAIIKQPDNISCFNDVYKLQKEYQEALILDNSNPDFIWIGEHQLCYTLGRGSNYDN
CCCCEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCEEEECCCEEEEEECCCCCCCC
LLFSLNDEKYDVFKIDRGGEVTCHMPGQLVTYLILDLKNFNKDLNWYLRKIEKIIIKILG
EEEEECCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
NFNIDCHLKKGFTGVWIENKKIASIGIGCRRWITINGFSINFNCELENFNKIVPCGIENC
CCCEEEEEECCCEEEEEECCEEEEECCCEEEEEEEECEEEEEEEEECCCCEEECCCCHHH
LMANMIDYNKNLNIKEVKKIVKKIIQEEFNFDFVSK
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA