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The map label for this gene is metK [H]

Identifier: 78778702

GI number: 78778702

Start: 302296

End: 303537

Strand: Reverse

Name: metK [H]

Synonym: PMT9312_0317

Alternate gene names: 78778702

Gene position: 303537-302296 (Counterclockwise)

Preceding gene: 78778703

Following gene: 78778701

Centisome position: 17.76

GC content: 36.71

Gene sequence:

>1242_bases
ATGAGTGATTTCATTTTCACTTCTGAATCCGTTACTGAAGGTCATCCTGACAAAATATGTGATCAAATTAGTGACGCAGT
TTTAGATGCTTTATTAACAGAAGATCCAGAAAGCAGAGTTGCATGCGAAACTGTCGTTAACACTGGTCTATGTCTACTTA
CTGGAGAAATAACTTCAAAAGCGAAAGTCGATTATATAAAACTTGTTAGAAATGTAATTAAAGAAATTGGATATGAAGGC
TATAGAGCAGGTGGTTTTGACGCAAATAGTTGTGCTGTTTTAGTAGCACTTGACGAGCAATCACCAGATATTTCACAAGG
AGTAAACTACGCAGATGATGTTAACGATGATTTAGAAGATAATACCGGAGCTGGTGACCAAGGCATAATGTTCGGCTATG
CATGCGATGAGACGCCTGAATTAATGCCATTACCAATTAGCTTGGCTCATAGATTAGCCATTCAACTTGCCAAAGTGAGA
CATGAAGAAGTCCTTAATTATCTACTCCCAGATGGTAAAACGCAAGTAAGCATTGATTACGAAAAAGGGTTACCAGTCTC
TATTAATACGATTTTAATTTCAACTCAACATAATCCTGAGATTGATGGAATAACAAATGAAGAAGAAATTCGTCAAAGAA
TAAAAGAAGATTTATGGACGCATGTTGTAATTCCTGCTACTGAAGATTTAAAAATCAAACCAAACATTAGCAAAACAAGA
TTTCTAGTAAACCCGACGGGCAAATTTGTAGTAGGCGGTCCTCAAGGAGATGCTGGGCTCACTGGTAGAAAAATTATTGT
AGATACTTATGGTGGATATGCAAGGCACGGAGGTGGTGCATTTTCTGGTAAAGATCCCACAAAAGTAGATAGATCAGCCG
CTTATGCAGCACGTTATGTAGCTAAAAGCATAGTTAAGGCAAAATTGGCAAAAAAAGCAGAAGTACAATTAAGTTATGCA
ATTGGAGTAGCAAAACCAATTTCCATTCTGGTGGAAACTTTTGATACTGGTGTTATTTCACAAGCTAATTTGACTGAGCT
AATTAAAGAGCACTTTGATTTAAGACCCGCAGCAATAATAAAAGAATTTAACTTAAGAAATTTACCAAAAAAAATGGGGG
GAAATTTTTTCAGAAAGACTGCATCCTATGGACATTTTGGCAGGCGAGATCTCCAGCTCCCTTGGGAAAATGTCGAAGAA
AAAGCAGCCAAATTAGAAGAGGCATCCAAAATATTTTTATAA

Upstream 100 bases:

>100_bases
TACAAACAAGTTCTTACAGGACAAATTTTTATTTACAATCATTGATTACAAACAACAGTTTAACTTAGGATAAGCCTTAG
TCTTAAAAAATTATATTTAA

Downstream 100 bases:

>100_bases
GGTATTGCTTCTATGCCTGATAATTATTATGGAGGGTTAGATTTTGGAACTAGTGGTGCGAGAATATCTATAATTGATCT
TTATAAAGAATCAGTATATT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSDFIFTSESVTEGHPDKICDQISDAVLDALLTEDPESRVACETVVNTGLCLLTGEITSKAKVDYIKLVRNVIKEIGYEG
YRAGGFDANSCAVLVALDEQSPDISQGVNYADDVNDDLEDNTGAGDQGIMFGYACDETPELMPLPISLAHRLAIQLAKVR
HEEVLNYLLPDGKTQVSIDYEKGLPVSINTILISTQHNPEIDGITNEEEIRQRIKEDLWTHVVIPATEDLKIKPNISKTR
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKSIVKAKLAKKAEVQLSYA
IGVAKPISILVETFDTGVISQANLTELIKEHFDLRPAAIIKEFNLRNLPKKMGGNFFRKTASYGHFGRRDLQLPWENVEE
KAAKLEEASKIFL

Sequences:

>Translated_413_residues
MSDFIFTSESVTEGHPDKICDQISDAVLDALLTEDPESRVACETVVNTGLCLLTGEITSKAKVDYIKLVRNVIKEIGYEG
YRAGGFDANSCAVLVALDEQSPDISQGVNYADDVNDDLEDNTGAGDQGIMFGYACDETPELMPLPISLAHRLAIQLAKVR
HEEVLNYLLPDGKTQVSIDYEKGLPVSINTILISTQHNPEIDGITNEEEIRQRIKEDLWTHVVIPATEDLKIKPNISKTR
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKSIVKAKLAKKAEVQLSYA
IGVAKPISILVETFDTGVISQANLTELIKEHFDLRPAAIIKEFNLRNLPKKMGGNFFRKTASYGHFGRRDLQLPWENVEE
KAAKLEEASKIFL
>Mature_412_residues
SDFIFTSESVTEGHPDKICDQISDAVLDALLTEDPESRVACETVVNTGLCLLTGEITSKAKVDYIKLVRNVIKEIGYEGY
RAGGFDANSCAVLVALDEQSPDISQGVNYADDVNDDLEDNTGAGDQGIMFGYACDETPELMPLPISLAHRLAIQLAKVRH
EEVLNYLLPDGKTQVSIDYEKGLPVSINTILISTQHNPEIDGITNEEEIRQRIKEDLWTHVVIPATEDLKIKPNISKTRF
LVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKSIVKAKLAKKAEVQLSYAI
GVAKPISILVETFDTGVISQANLTELIKEHFDLRPAAIIKEFNLRNLPKKMGGNFFRKTASYGHFGRRDLQLPWENVEEK
AAKLEEASKIFL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=397, Percent_Identity=54.4080604534005, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI5174529, Length=397, Percent_Identity=54.1561712846348, Blast_Score=388, Evalue=1e-108,
Organism=Escherichia coli, GI1789311, Length=411, Percent_Identity=54.5012165450122, Blast_Score=424, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25145633, Length=403, Percent_Identity=54.3424317617866, Blast_Score=393, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17551082, Length=411, Percent_Identity=53.2846715328467, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17538498, Length=400, Percent_Identity=54, Blast_Score=388, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17538494, Length=414, Percent_Identity=52.4154589371981, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32565905, Length=414, Percent_Identity=45.8937198067633, Blast_Score=321, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI25145635, Length=357, Percent_Identity=51.5406162464986, Blast_Score=316, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI32565911, Length=354, Percent_Identity=51.1299435028249, Blast_Score=311, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI32565903, Length=354, Percent_Identity=46.6101694915254, Blast_Score=277, Evalue=7e-75,
Organism=Caenorhabditis elegans, GI32565909, Length=81, Percent_Identity=70.3703703703704, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32565907, Length=39, Percent_Identity=76.9230769230769, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=398, Percent_Identity=51.2562814070352, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6320710, Length=407, Percent_Identity=50.8599508599509, Blast_Score=382, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45552159, Length=397, Percent_Identity=52.896725440806, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580529, Length=397, Percent_Identity=52.896725440806, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580537, Length=397, Percent_Identity=52.896725440806, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580533, Length=397, Percent_Identity=52.896725440806, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580535, Length=397, Percent_Identity=52.896725440806, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580531, Length=397, Percent_Identity=52.896725440806, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580527, Length=397, Percent_Identity=52.6448362720403, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24580525, Length=397, Percent_Identity=52.6448362720403, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24580541, Length=384, Percent_Identity=51.5625, Blast_Score=335, Evalue=3e-92,
Organism=Drosophila melanogaster, GI24580539, Length=124, Percent_Identity=58.0645161290323, Blast_Score=139, Evalue=3e-33,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 45271; Mature: 45140

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFIFTSESVTEGHPDKICDQISDAVLDALLTEDPESRVACETVVNTGLCLLTGEITSK
CCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCCCC
AKVDYIKLVRNVIKEIGYEGYRAGGFDANSCAVLVALDEQSPDISQGVNYADDVNDDLED
HHHHHHHHHHHHHHHCCCCCEECCCCCCCCCEEEEEECCCCCCHHHCCCCCCCCCCCCCC
NTGAGDQGIMFGYACDETPELMPLPISLAHRLAIQLAKVRHEEVLNYLLPDGKTQVSIDY
CCCCCCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
EKGLPVSINTILISTQHNPEIDGITNEEEIRQRIKEDLWTHVVIPATEDLKIKPNISKTR
CCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCEEEEECCCCCEECCCCCCEE
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYV
EEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AKSIVKAKLAKKAEVQLSYAIGVAKPISILVETFDTGVISQANLTELIKEHFDLRPAAII
HHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHH
KEFNLRNLPKKMGGNFFRKTASYGHFGRRDLQLPWENVEEKAAKLEEASKIFL
HHCCHHCCCHHHCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDFIFTSESVTEGHPDKICDQISDAVLDALLTEDPESRVACETVVNTGLCLLTGEITSK
CCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCCCC
AKVDYIKLVRNVIKEIGYEGYRAGGFDANSCAVLVALDEQSPDISQGVNYADDVNDDLED
HHHHHHHHHHHHHHHCCCCCEECCCCCCCCCEEEEEECCCCCCHHHCCCCCCCCCCCCCC
NTGAGDQGIMFGYACDETPELMPLPISLAHRLAIQLAKVRHEEVLNYLLPDGKTQVSIDY
CCCCCCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
EKGLPVSINTILISTQHNPEIDGITNEEEIRQRIKEDLWTHVVIPATEDLKIKPNISKTR
CCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCEEEEECCCCCEECCCCCCEE
FLVNPTGKFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYV
EEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AKSIVKAKLAKKAEVQLSYAIGVAKPISILVETFDTGVISQANLTELIKEHFDLRPAAII
HHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHH
KEFNLRNLPKKMGGNFFRKTASYGHFGRRDLQLPWENVEEKAAKLEEASKIFL
HHCCHHCCCHHHCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA