Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 78484802

GI number: 78484802

Start: 514339

End: 515388

Strand: Reverse

Name: pleD [H]

Synonym: Tcr_0457

Alternate gene names: 78484802

Gene position: 515388-514339 (Counterclockwise)

Preceding gene: 78484804

Following gene: 78484801

Centisome position: 21.23

GC content: 40.67

Gene sequence:

>1050_bases
ATGAATTCATCGCAATTAATCCAAAGCCCTTGTCGTTATAGCTTCTCGAAGGAAGAAGCTCGCGAATTAGCCGAACAAAC
GCTAGAAAAACTCAATCATCATGACATCAGTGCGAATCCTATCCATTTCACACTTTTCTTTGAACTCTTAACGTCAATCA
ATCCCAGTATCGCAGAACAAGTTGAGCACGCATTACAATTTAAAACGTATAACGACGACTCAGCTCGCGTCATGTTCAAT
GCACTTTGGAGTGAAATCATCAGTCATAGTTTACACTCTGAAGAGTTTTCAGAGATTGTGGATAATTTAATTTTATATAT
CGACAATTGGATTAAAGCCGCCCAAAACAAGTCAGACACCATCAGCGCCGAAGTCGAGAAAGTATCACAACTCAGCACAG
CGCAAGAGATGATTGATCGCCTGAAATCCAATGTATTGCCTGCCATAGCATCTTACCAGTCTGAAACGAATGCTTTACAA
GAGCAGATTCAAGATTCAAGCGCCGAAATTATGCGACTCAAAAAAGAGCTCGACAAGGCCACGGCCATTGCTAAAACCGA
TGAATTGACTAACATTCCTAATCGACGTGGCTTTAATGAAATCATTGAAAACATGATGAAAGAAGCCAACGAAAAACAAA
GCAGTTTTGCACTGTTAGTATTGGACATTGATTTCTTTAAAAAAGTTAATGATACCTATGGGCATTTGGTTGGGGACAGC
ATTTTAAGATACCTCGCCAAAAGCCTGCATAACGAAACCAAAGGAAAAGATTCAATTGCACGTATCGGGGGCGAAGAGTT
TGTGGCCATTTTACCGAGCACCAGCTACGACTCGGCTTTAAAATTAGCCAACACCATTCGTGAAAAAATTGCTTCACGCC
CGCTTCATGTCAAAAATAATGCAAACCCGATTACCCTGACTATTTCAATAGGTGTAGCCATGTTCCAGCTCAATGAAACA
TTAGACTCACTCTTTACGCGAGCAGATAAATGCCTTTACATGGCTAAATCAAATGGCCGAAATCGAGTGTATGGTGAAGC
CGAATTATAA

Upstream 100 bases:

>100_bases
CTTATAAAATCACGCTTTTAGGTGTTAATCCACTTAAAAAGTCTATAATATTATCTATATATAAATAACAAAACGCACTC
ACTAAAGATTTAGGTTATCA

Downstream 100 bases:

>100_bases
CGCCTAGCTGACAGCTTTCAAATGACGTCAAAGACCGCACTCAATATTATTTCTGGTCAACTAGGGGCCGGGAAAACCAC
CCTGCTCCAACAGTTACTCC

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MNSSQLIQSPCRYSFSKEEARELAEQTLEKLNHHDISANPIHFTLFFELLTSINPSIAEQVEHALQFKTYNDDSARVMFN
ALWSEIISHSLHSEEFSEIVDNLILYIDNWIKAAQNKSDTISAEVEKVSQLSTAQEMIDRLKSNVLPAIASYQSETNALQ
EQIQDSSAEIMRLKKELDKATAIAKTDELTNIPNRRGFNEIIENMMKEANEKQSSFALLVLDIDFFKKVNDTYGHLVGDS
ILRYLAKSLHNETKGKDSIARIGGEEFVAILPSTSYDSALKLANTIREKIASRPLHVKNNANPITLTISIGVAMFQLNET
LDSLFTRADKCLYMAKSNGRNRVYGEAEL

Sequences:

>Translated_349_residues
MNSSQLIQSPCRYSFSKEEARELAEQTLEKLNHHDISANPIHFTLFFELLTSINPSIAEQVEHALQFKTYNDDSARVMFN
ALWSEIISHSLHSEEFSEIVDNLILYIDNWIKAAQNKSDTISAEVEKVSQLSTAQEMIDRLKSNVLPAIASYQSETNALQ
EQIQDSSAEIMRLKKELDKATAIAKTDELTNIPNRRGFNEIIENMMKEANEKQSSFALLVLDIDFFKKVNDTYGHLVGDS
ILRYLAKSLHNETKGKDSIARIGGEEFVAILPSTSYDSALKLANTIREKIASRPLHVKNNANPITLTISIGVAMFQLNET
LDSLFTRADKCLYMAKSNGRNRVYGEAEL
>Mature_349_residues
MNSSQLIQSPCRYSFSKEEARELAEQTLEKLNHHDISANPIHFTLFFELLTSINPSIAEQVEHALQFKTYNDDSARVMFN
ALWSEIISHSLHSEEFSEIVDNLILYIDNWIKAAQNKSDTISAEVEKVSQLSTAQEMIDRLKSNVLPAIASYQSETNALQ
EQIQDSSAEIMRLKKELDKATAIAKTDELTNIPNRRGFNEIIENMMKEANEKQSSFALLVLDIDFFKKVNDTYGHLVGDS
ILRYLAKSLHNETKGKDSIARIGGEEFVAILPSTSYDSALKLANTIREKIASRPLHVKNNANPITLTISIGVAMFQLNET
LDSLFTRADKCLYMAKSNGRNRVYGEAEL

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=43.0232558139535, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI145693134, Length=184, Percent_Identity=34.7826086956522, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787816, Length=223, Percent_Identity=30.9417040358744, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787802, Length=173, Percent_Identity=35.2601156069364, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=38.0368098159509, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=34.5454545454545, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786584, Length=202, Percent_Identity=33.1683168316832, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788381, Length=171, Percent_Identity=30.4093567251462, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787541, Length=178, Percent_Identity=28.0898876404494, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788956, Length=182, Percent_Identity=31.3186813186813, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788085, Length=179, Percent_Identity=31.2849162011173, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87081974, Length=157, Percent_Identity=29.2993630573248, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787056, Length=163, Percent_Identity=33.7423312883436, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081921, Length=228, Percent_Identity=22.8070175438596, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081977, Length=151, Percent_Identity=28.476821192053, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 39384; Mature: 39384

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSQLIQSPCRYSFSKEEARELAEQTLEKLNHHDISANPIHFTLFFELLTSINPSIAEQ
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHH
VEHALQFKTYNDDSARVMFNALWSEIISHSLHSEEFSEIVDNLILYIDNWIKAAQNKSDT
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
ISAEVEKVSQLSTAQEMIDRLKSNVLPAIASYQSETNALQEQIQDSSAEIMRLKKELDKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAIAKTDELTNIPNRRGFNEIIENMMKEANEKQSSFALLVLDIDFFKKVNDTYGHLVGDS
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCEEEEEEEHHHHHHHHHHHHHHHHHH
ILRYLAKSLHNETKGKDSIARIGGEEFVAILPSTSYDSALKLANTIREKIASRPLHVKNN
HHHHHHHHHHCCCCCHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEECCC
ANPITLTISIGVAMFQLNETLDSLFTRADKCLYMAKSNGRNRVYGEAEL
CCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MNSSQLIQSPCRYSFSKEEARELAEQTLEKLNHHDISANPIHFTLFFELLTSINPSIAEQ
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHH
VEHALQFKTYNDDSARVMFNALWSEIISHSLHSEEFSEIVDNLILYIDNWIKAAQNKSDT
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
ISAEVEKVSQLSTAQEMIDRLKSNVLPAIASYQSETNALQEQIQDSSAEIMRLKKELDKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAIAKTDELTNIPNRRGFNEIIENMMKEANEKQSSFALLVLDIDFFKKVNDTYGHLVGDS
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCEEEEEEEHHHHHHHHHHHHHHHHHH
ILRYLAKSLHNETKGKDSIARIGGEEFVAILPSTSYDSALKLANTIREKIASRPLHVKNN
HHHHHHHHHHCCCCCHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEECCC
ANPITLTISIGVAMFQLNETLDSLFTRADKCLYMAKSNGRNRVYGEAEL
CCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA