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The map label for this gene is purD [H]

Identifier: 78484783

GI number: 78484783

Start: 490668

End: 491960

Strand: Reverse

Name: purD [H]

Synonym: Tcr_0438

Alternate gene names: 78484783

Gene position: 491960-490668 (Counterclockwise)

Preceding gene: 78484784

Following gene: 78484782

Centisome position: 20.26

GC content: 47.72

Gene sequence:

>1293_bases
ATGAACGTACTCATTATTGGAAGCGGTGGTCGTGAACATGCCTTGGCATGGAAAACCATTCAATCAGATAAAGTTTCAAA
GGTATTTGTCGCACCGGGCAATGCTGGTACAGCACTGGAAGACAATATCGAAAATGTTGATATTGCTGTCGAAGATCTGC
CAGGCCTGGTCGATTTTGCTCAAAAAAATGCCATCGAACTCACCATTGTTGGCCCGGAAGTGCCTCTTGTTTTAGGCGTG
GTTGACGCCTTTCAAGACGCCGGCCTAAAATGTTTTGGCCCAAGCAAAGGAGCGGCGCAGTTGGAAGGCTCCAAAGCGTT
CTCTAAAGATTTTCTAGCCAAGCATAACATTCCAACAGCAGCCTATTCTGTTTTCACTGAAATCCCACCCGCGGTGGCAT
ATATTGAAAAAATGGGCGCCCCAATCGTCATCAAAGCGGATGGTTTAGCGGCTGGAAAAGGCGTTATTCTGGCGGAAACC
AATGCCGAAGCGATTGCGGCCGTTGAAGACATGTTGGCCGGTAATAAGTTTGGCGACGCTGGAGCACGGGTTGTTATTGA
AGAATTTCTAGTCGGTGAAGAAGCCAGCTTTATCGTCATGGCCGATGGTAAAAACATCTTACCAATGGCAACCTCTCAAG
ACCATAAAGCCAGAAATAATGGGGATACTGGACCTAACACAGGTGGCATGGGCGCTTATACACCGGCACCAGTGGTAACA
CGCAAAATCCATGACCGCATTATGCAGGAAGTGATTCGCCCAACCATTGACGGAATGGCTAAAGACGGCTTACCGTACAC
GGGTTTTTTATATGCTGGAATCATGATTGCAGAGGATGGAACCCCTAAGGTACTTGAGTTTAACTGTCGCTTTGGTGATC
CGGAAACACAACCTATTATGATGCGCTTACAATCCGACTTATCTGAACTGTGTTTAGCGGCACTGGATAAAAAATTGGAT
ACAGTGACAGCCAAATGGGATTCTCGTCCTGCATTAGGTGTTGTTTTGGCTGCGGGAGGGTACCCTGATGAATATCGCAA
AGGCGACATTATTGAAGGCATTGAAGACGCCAACAATACGGACGTGAAAGTTTTTCACGCGGGCACTCGCTTAGATGAAC
AGGGGCAATTGGTGACCTCAGGTGGACGTGTTTTATGCGTCACTGCCTTGGGCGAAAACGTAACAGACGCTCAAAAACGA
GCCTATGAAGGGGTTGCGAAAATCCACTGGAATGAAGTCTATTACCGTACCGACATTGGTCACCGTGCCGTGAAGCGCGA
AGCCATCAGTTAA

Upstream 100 bases:

>100_bases
CGGATGAGCACGGCATCGCGATGGTCTTCACTGGCATGCGTCACTTTAAACACTAAAGACACTGATTGAAATACGATAGA
TTTATAAGAGAGAAAAACAC

Downstream 100 bases:

>100_bases
CAAAGCGATTGTTTCTTTCCTAAAAAGCACTCATCGAGTGCTTTTTTTATGCCTGCATTTTCGGTATAGTTAAATAATGG
TTATTCATTAAGGAATTTTA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNVLIIGSGGREHALAWKTIQSDKVSKVFVAPGNAGTALEDNIENVDIAVEDLPGLVDFAQKNAIELTIVGPEVPLVLGV
VDAFQDAGLKCFGPSKGAAQLEGSKAFSKDFLAKHNIPTAAYSVFTEIPPAVAYIEKMGAPIVIKADGLAAGKGVILAET
NAEAIAAVEDMLAGNKFGDAGARVVIEEFLVGEEASFIVMADGKNILPMATSQDHKARNNGDTGPNTGGMGAYTPAPVVT
RKIHDRIMQEVIRPTIDGMAKDGLPYTGFLYAGIMIAEDGTPKVLEFNCRFGDPETQPIMMRLQSDLSELCLAALDKKLD
TVTAKWDSRPALGVVLAAGGYPDEYRKGDIIEGIEDANNTDVKVFHAGTRLDEQGQLVTSGGRVLCVTALGENVTDAQKR
AYEGVAKIHWNEVYYRTDIGHRAVKREAIS

Sequences:

>Translated_430_residues
MNVLIIGSGGREHALAWKTIQSDKVSKVFVAPGNAGTALEDNIENVDIAVEDLPGLVDFAQKNAIELTIVGPEVPLVLGV
VDAFQDAGLKCFGPSKGAAQLEGSKAFSKDFLAKHNIPTAAYSVFTEIPPAVAYIEKMGAPIVIKADGLAAGKGVILAET
NAEAIAAVEDMLAGNKFGDAGARVVIEEFLVGEEASFIVMADGKNILPMATSQDHKARNNGDTGPNTGGMGAYTPAPVVT
RKIHDRIMQEVIRPTIDGMAKDGLPYTGFLYAGIMIAEDGTPKVLEFNCRFGDPETQPIMMRLQSDLSELCLAALDKKLD
TVTAKWDSRPALGVVLAAGGYPDEYRKGDIIEGIEDANNTDVKVFHAGTRLDEQGQLVTSGGRVLCVTALGENVTDAQKR
AYEGVAKIHWNEVYYRTDIGHRAVKREAIS
>Mature_430_residues
MNVLIIGSGGREHALAWKTIQSDKVSKVFVAPGNAGTALEDNIENVDIAVEDLPGLVDFAQKNAIELTIVGPEVPLVLGV
VDAFQDAGLKCFGPSKGAAQLEGSKAFSKDFLAKHNIPTAAYSVFTEIPPAVAYIEKMGAPIVIKADGLAAGKGVILAET
NAEAIAAVEDMLAGNKFGDAGARVVIEEFLVGEEASFIVMADGKNILPMATSQDHKARNNGDTGPNTGGMGAYTPAPVVT
RKIHDRIMQEVIRPTIDGMAKDGLPYTGFLYAGIMIAEDGTPKVLEFNCRFGDPETQPIMMRLQSDLSELCLAALDKKLD
TVTAKWDSRPALGVVLAAGGYPDEYRKGDIIEGIEDANNTDVKVFHAGTRLDEQGQLVTSGGRVLCVTALGENVTDAQKR
AYEGVAKIHWNEVYYRTDIGHRAVKREAIS

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=50.3529411764706, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=50.3529411764706, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=50.3529411764706, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=50.3529411764706, Blast_Score=412, Evalue=1e-115,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=67.4473067915691, Blast_Score=592, Evalue=1e-170,
Organism=Caenorhabditis elegans, GI17567511, Length=425, Percent_Identity=44.7058823529412, Blast_Score=322, Evalue=3e-88,
Organism=Saccharomyces cerevisiae, GI6321203, Length=445, Percent_Identity=44.7191011235955, Blast_Score=369, Evalue=1e-103,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=44.5475638051044, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=44.5475638051044, Blast_Score=352, Evalue=4e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45798; Mature: 45798

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLIIGSGGREHALAWKTIQSDKVSKVFVAPGNAGTALEDNIENVDIAVEDLPGLVDFA
CEEEEEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEEHHCCCHHHHH
QKNAIELTIVGPEVPLVLGVVDAFQDAGLKCFGPSKGAAQLEGSKAFSKDFLAKHNIPTA
CCCCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCHH
AYSVFTEIPPAVAYIEKMGAPIVIKADGLAAGKGVILAETNAEAIAAVEDMLAGNKFGDA
HHHHHHHCCHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCC
GARVVIEEFLVGEEASFIVMADGKNILPMATSQDHKARNNGDTGPNTGGMGAYTPAPVVT
HHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
RKIHDRIMQEVIRPTIDGMAKDGLPYTGFLYAGIMIAEDGTPKVLEFNCRFGDPETQPIM
HHHHHHHHHHHHHHHHHHHHHCCCCEEEHEEEEEEEECCCCCCEEEEEECCCCCCCCHHH
MRLQSDLSELCLAALDKKLDTVTAKWDSRPALGVVLAAGGYPDEYRKGDIIEGIEDANNT
HHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEECCCCCHHHCCCCHHHCCCCCCCC
DVKVFHAGTRLDEQGQLVTSGGRVLCVTALGENVTDAQKRAYEGVAKIHWNEVYYRTDIG
CEEEEECCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHCHHEEEEEEEEEEECCC
HRAVKREAIS
HHHHHHHCCC
>Mature Secondary Structure
MNVLIIGSGGREHALAWKTIQSDKVSKVFVAPGNAGTALEDNIENVDIAVEDLPGLVDFA
CEEEEEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEEHHCCCHHHHH
QKNAIELTIVGPEVPLVLGVVDAFQDAGLKCFGPSKGAAQLEGSKAFSKDFLAKHNIPTA
CCCCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCHH
AYSVFTEIPPAVAYIEKMGAPIVIKADGLAAGKGVILAETNAEAIAAVEDMLAGNKFGDA
HHHHHHHCCHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCC
GARVVIEEFLVGEEASFIVMADGKNILPMATSQDHKARNNGDTGPNTGGMGAYTPAPVVT
HHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
RKIHDRIMQEVIRPTIDGMAKDGLPYTGFLYAGIMIAEDGTPKVLEFNCRFGDPETQPIM
HHHHHHHHHHHHHHHHHHHHHCCCCEEEHEEEEEEEECCCCCCEEEEEECCCCCCCCHHH
MRLQSDLSELCLAALDKKLDTVTAKWDSRPALGVVLAAGGYPDEYRKGDIIEGIEDANNT
HHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEECCCCCHHHCCCCHHHCCCCCCCC
DVKVFHAGTRLDEQGQLVTSGGRVLCVTALGENVTDAQKRAYEGVAKIHWNEVYYRTDIG
CEEEEECCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHCHHEEEEEEEEEEECCC
HRAVKREAIS
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]