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The map label for this gene is prfA

Identifier: 78484734

GI number: 78484734

Start: 438424

End: 439515

Strand: Reverse

Name: prfA

Synonym: Tcr_0389

Alternate gene names: 78484734

Gene position: 439515-438424 (Counterclockwise)

Preceding gene: 78484735

Following gene: 78484733

Centisome position: 18.1

GC content: 48.63

Gene sequence:

>1092_bases
ATGAAAGAATCCATTCGCAGCAAACTTGAAAACCTGGTCGAACGACTCGATGAAGTGAACCATCTTATTTCCGACCCGGA
AATCATCAACAACCAGAACAAGTTTCGTAACCTGACCAAAGAGCACTCTCAATTAACCCCGGTGGTTGAAACCTTTAACC
AATTTATTCAAAACCAAGGCGACTTGCAGGAAGCTAAAGACATGATTAAATCAGGCGACCCGGATTTAAAAGAAATGGGT
CAGGAAGAATTGCCGGAGCTTGAAAAGCAACAAGCGACCTTGGAAGCGGAATTGCAAAAAATGCTGCTGCCGAAAGATCC
GAACGATGATGCTAATATCTTCTTAGAGATTCGCGCCGGAACAGGTGGGGATGAAGCGGCGATCTTTGCCGGTGATCTTT
TTCGAATGTATAGCCGCTATGCTGAAACAATGCGCTGGCAGGTTGAAGTCATCAACTCTCAAGAAGGTGAGCACGGAGGC
TACAAAGAAATTATTGCTCGTATCATCGGAGACGGCGCTTATTCTCGTTTGAAGTTTGAGTCCGGTGCTCATCGAGTGCA
ACGGGTTCCAGCCACTGAAACGCAAGGACGCGTGCACACCTCTGCCGCAACGGTGGTCATCATGCCGGAAGCTCAGGATG
TGGAACAAATCGATTTAAACCCTGCGGATTTAAAAGTCGATACCTTCCGTGCATCCGGTGCCGGTGGGCAGCATGTTAAC
AAAACCGATTCCGCCATTCGAATCACCCATATTCCAACGGGAACCGTCGTGGAATGTCAGGACGAGCGCTCTCAGCATAA
AAACCGTGCTCGAGCCATGTCATTACTGGCTTCGCGCATCATGGACGAGCGTCAGCGCATCCATGACCAGGAAATTGCAC
AAGAACGTAAAAGTCTGGTTGGCAGTGGTGACCGCTCCGAACGCATTCGAACCTACAACTATCCGCAAGGCCGTGTTACC
GATCACCGCATCAACTTGACACTGTATAAGCTGGATGAAATCATGCAGGGCGGCTTGCAACAAGTGGTTGACCCTTTGAT
TAACGAATATCAAGCTGAACAACTGGCAGCACTTAGCGAAGACAGTGCTTAA

Upstream 100 bases:

>100_bases
ATTTTATTGACCCAACAGTCAGACGCCAGTGATTCTTCAGAAAACGAAAACACACACGCAACGTCTTAACTTTTAACCTG
ACTCCCAAAAAAGAATTCCT

Downstream 100 bases:

>100_bases
GCGACCTAAGCCATTAAAAGAGAGGCGGCATGCGAATTGATGAGGCTTTAACTGCGGCGAAGCAACAGCTGAAAGCCGCC
TCCTCCGACAGCATTGCGCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKESIRSKLENLVERLDEVNHLISDPEIINNQNKFRNLTKEHSQLTPVVETFNQFIQNQGDLQEAKDMIKSGDPDLKEMG
QEELPELEKQQATLEAELQKMLLPKDPNDDANIFLEIRAGTGGDEAAIFAGDLFRMYSRYAETMRWQVEVINSQEGEHGG
YKEIIARIIGDGAYSRLKFESGAHRVQRVPATETQGRVHTSAATVVIMPEAQDVEQIDLNPADLKVDTFRASGAGGQHVN
KTDSAIRITHIPTGTVVECQDERSQHKNRARAMSLLASRIMDERQRIHDQEIAQERKSLVGSGDRSERIRTYNYPQGRVT
DHRINLTLYKLDEIMQGGLQQVVDPLINEYQAEQLAALSEDSA

Sequences:

>Translated_363_residues
MKESIRSKLENLVERLDEVNHLISDPEIINNQNKFRNLTKEHSQLTPVVETFNQFIQNQGDLQEAKDMIKSGDPDLKEMG
QEELPELEKQQATLEAELQKMLLPKDPNDDANIFLEIRAGTGGDEAAIFAGDLFRMYSRYAETMRWQVEVINSQEGEHGG
YKEIIARIIGDGAYSRLKFESGAHRVQRVPATETQGRVHTSAATVVIMPEAQDVEQIDLNPADLKVDTFRASGAGGQHVN
KTDSAIRITHIPTGTVVECQDERSQHKNRARAMSLLASRIMDERQRIHDQEIAQERKSLVGSGDRSERIRTYNYPQGRVT
DHRINLTLYKLDEIMQGGLQQVVDPLINEYQAEQLAALSEDSA
>Mature_363_residues
MKESIRSKLENLVERLDEVNHLISDPEIINNQNKFRNLTKEHSQLTPVVETFNQFIQNQGDLQEAKDMIKSGDPDLKEMG
QEELPELEKQQATLEAELQKMLLPKDPNDDANIFLEIRAGTGGDEAAIFAGDLFRMYSRYAETMRWQVEVINSQEGEHGG
YKEIIARIIGDGAYSRLKFESGAHRVQRVPATETQGRVHTSAATVVIMPEAQDVEQIDLNPADLKVDTFRASGAGGQHVN
KTDSAIRITHIPTGTVVECQDERSQHKNRARAMSLLASRIMDERQRIHDQEIAQERKSLVGSGDRSERIRTYNYPQGRVT
DHRINLTLYKLDEIMQGGLQQVVDPLINEYQAEQLAALSEDSA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=296, Percent_Identity=44.9324324324324, Blast_Score=261, Evalue=8e-70,
Organism=Homo sapiens, GI34577120, Length=344, Percent_Identity=39.8255813953488, Blast_Score=248, Evalue=6e-66,
Organism=Homo sapiens, GI166795305, Length=210, Percent_Identity=41.9047619047619, Blast_Score=181, Evalue=1e-45,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=62.7777777777778, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=37.125748502994, Blast_Score=221, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI17542784, Length=297, Percent_Identity=34.3434343434343, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321295, Length=309, Percent_Identity=43.6893203883495, Blast_Score=257, Evalue=2e-69,
Organism=Drosophila melanogaster, GI19921226, Length=323, Percent_Identity=41.1764705882353, Blast_Score=227, Evalue=1e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_THICR (Q31IN8)

Other databases:

- EMBL:   CP000109
- RefSeq:   YP_390659.1
- HSSP:   P0A7I0
- ProteinModelPortal:   Q31IN8
- SMR:   Q31IN8
- STRING:   Q31IN8
- GeneID:   3760904
- GenomeReviews:   CP000109_GR
- KEGG:   tcx:Tcr_0389
- NMPDR:   fig|317025.3.peg.358
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   GEAGGYK
- PhylomeDB:   Q31IN8
- BioCyc:   TCRU317025:TCR_0389-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41002; Mature: 41002

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKESIRSKLENLVERLDEVNHLISDPEIINNQNKFRNLTKEHSQLTPVVETFNQFIQNQG
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCC
DLQEAKDMIKSGDPDLKEMGQEELPELEKQQATLEAELQKMLLPKDPNDDANIFLEIRAG
CHHHHHHHHHCCCCCHHHCCHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECC
TGGDEAAIFAGDLFRMYSRYAETMRWQVEVINSQEGEHGGYKEIIARIIGDGAYSRLKFE
CCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHCCCHHHHHEEH
SGAHRVQRVPATETQGRVHTSAATVVIMPEAQDVEQIDLNPADLKVDTFRASGAGGQHVN
HHHHHHHCCCCCCCCCCEEECCEEEEEECCCCCHHHCCCCCCCEEEEEEECCCCCCCCCC
KTDSAIRITHIPTGTVVECQDERSQHKNRARAMSLLASRIMDERQRIHDQEIAQERKSLV
CCCCEEEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSGDRSERIRTYNYPQGRVTDHRINLTLYKLDEIMQGGLQQVVDPLINEYQAEQLAALSE
CCCCCCCCCEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DSA
CCC
>Mature Secondary Structure
MKESIRSKLENLVERLDEVNHLISDPEIINNQNKFRNLTKEHSQLTPVVETFNQFIQNQG
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCC
DLQEAKDMIKSGDPDLKEMGQEELPELEKQQATLEAELQKMLLPKDPNDDANIFLEIRAG
CHHHHHHHHHCCCCCHHHCCHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECC
TGGDEAAIFAGDLFRMYSRYAETMRWQVEVINSQEGEHGGYKEIIARIIGDGAYSRLKFE
CCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHCCCHHHHHEEH
SGAHRVQRVPATETQGRVHTSAATVVIMPEAQDVEQIDLNPADLKVDTFRASGAGGQHVN
HHHHHHHCCCCCCCCCCEEECCEEEEEECCCCCHHHCCCCCCCEEEEEEECCCCCCCCCC
KTDSAIRITHIPTGTVVECQDERSQHKNRARAMSLLASRIMDERQRIHDQEIAQERKSLV
CCCCEEEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSGDRSERIRTYNYPQGRVTDHRINLTLYKLDEIMQGGLQQVVDPLINEYQAEQLAALSE
CCCCCCCCCEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DSA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA