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The map label for this gene is bioB [H]

Identifier: 78484622

GI number: 78484622

Start: 324388

End: 325680

Strand: Reverse

Name: bioB [H]

Synonym: Tcr_0277

Alternate gene names: 78484622

Gene position: 325680-324388 (Counterclockwise)

Preceding gene: 78484624

Following gene: 78484621

Centisome position: 13.41

GC content: 47.1

Gene sequence:

>1293_bases
ATGACTCACTGGCAAGCACTTCTACAATTTGATCAAGAACACATTTGGCACCCTTACGCTAAAATGCCCTCTGACACTCC
CGCCATCGGCGTCGCTAAAACCCAAGGTTCCATCATTACCTTATCTGACGGTAACGAGCTCGTGGACGGTATGAGTTCAT
GGTGGGCCGCATTACATGGTTACAATCACCCGGTCATTCAAAAAGCGATGATTGATCAGATCGAAACCATGCCTCATATC
ATGTTTGGAGGCTTAACCCATGAACCTGCTATTGAGCTGGCAAAACGTCTCATTAAGCTGACGCCCCCGCCATTACAAAA
AGTATTTTTTACGGATTCGGGTTCGGTCGCAATGGAAGTGGCCATAAAAATGGCTATGCAATACTGGATTAGCTTAGAGC
AACCCCAAAAAAATAAACTTTTAGCCCTCAAGAATGGTTACCACGGGGATACGTTTGCCACCATGTCCATCTGCGACCCG
GATAATGGCATGCACCGTTTATTTAACAGTACATTGCCCCAGCACTTTTTTGCGCCCGCTCCGCAAAAAGGGTTCGATAT
TGACTCGGACAATCAAGACATTGCCGAATTTGAAACATTACTTGAAAAACATCACGCTGAAATAGCCGCGGTGACCTTGG
AACCTATTGTGCAAGGTGCTGGAGGGATGCGTTTCTATCGCCCAGACTATCTACGCCAAGTTCGTGAACTGTGCGATGAA
TTTAACGTATTACTCATTGCAGATGAAATCGCCACAGGGTTTGGTCGAACTGGAAAACTATTTGCCTGCGAATGGGCCAA
CATCAGCCCCGACATTATGGCACTGGGCAAAACACTGACCGGCGGCAACATCACGCTCGCGGCCACCCTGGCCACTGAAA
AAATCAGCGATACCATCAGTCAGGGCAACCCAGGCTTACTCATGCATGGCCCAACTTTTATGGCCAATCCACTTGCTTGT
CGAGCCGCATCCGCCAATATCGACGTATTATTGAACTCTCCATGGCAAGATAATATTCAACGGATTGAATCACACTTTCT
CAATGAACTAATGCCTTTAAACGCATTGCCTGGGGTTGACGATACGCGTGTTTTAGGTGCGATAGGCGTCATTGAACTGG
AGCGCAATGATTTAGGGGAAACCATCCAAAATCTTGCCGTTCAAAACGGTGTCTGGATCCGTCCTTTTGGCAAGCTTATT
TACACCATGCCAGCCTATAACATCCCCACAAACCAATTAACACAGTTAACTGAGGGTCTAAAAACCGCCATCGTTCAGGC
ATTGCAACACTAG

Upstream 100 bases:

>100_bases
CACTATGTTAGTCTCTTTTTTAAAACTTAATTTTTAGGATAATGAAACTCTGGTATGTTTTCGTTATCATACCCAAACGA
TAAATGACTGAAATATAAAT

Downstream 100 bases:

>100_bases
TACAAAAAGCCCGTCCAAATCTTTAAAAGAATGCTTTCTTTTTGATAGACTTCAATTAAAATTGAAGTCTTCGCTTCTTA
CCCTTCTTATTCTTATATAG

Product: adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTHWQALLQFDQEHIWHPYAKMPSDTPAIGVAKTQGSIITLSDGNELVDGMSSWWAALHGYNHPVIQKAMIDQIETMPHI
MFGGLTHEPAIELAKRLIKLTPPPLQKVFFTDSGSVAMEVAIKMAMQYWISLEQPQKNKLLALKNGYHGDTFATMSICDP
DNGMHRLFNSTLPQHFFAPAPQKGFDIDSDNQDIAEFETLLEKHHAEIAAVTLEPIVQGAGGMRFYRPDYLRQVRELCDE
FNVLLIADEIATGFGRTGKLFACEWANISPDIMALGKTLTGGNITLAATLATEKISDTISQGNPGLLMHGPTFMANPLAC
RAASANIDVLLNSPWQDNIQRIESHFLNELMPLNALPGVDDTRVLGAIGVIELERNDLGETIQNLAVQNGVWIRPFGKLI
YTMPAYNIPTNQLTQLTEGLKTAIVQALQH

Sequences:

>Translated_430_residues
MTHWQALLQFDQEHIWHPYAKMPSDTPAIGVAKTQGSIITLSDGNELVDGMSSWWAALHGYNHPVIQKAMIDQIETMPHI
MFGGLTHEPAIELAKRLIKLTPPPLQKVFFTDSGSVAMEVAIKMAMQYWISLEQPQKNKLLALKNGYHGDTFATMSICDP
DNGMHRLFNSTLPQHFFAPAPQKGFDIDSDNQDIAEFETLLEKHHAEIAAVTLEPIVQGAGGMRFYRPDYLRQVRELCDE
FNVLLIADEIATGFGRTGKLFACEWANISPDIMALGKTLTGGNITLAATLATEKISDTISQGNPGLLMHGPTFMANPLAC
RAASANIDVLLNSPWQDNIQRIESHFLNELMPLNALPGVDDTRVLGAIGVIELERNDLGETIQNLAVQNGVWIRPFGKLI
YTMPAYNIPTNQLTQLTEGLKTAIVQALQH
>Mature_429_residues
THWQALLQFDQEHIWHPYAKMPSDTPAIGVAKTQGSIITLSDGNELVDGMSSWWAALHGYNHPVIQKAMIDQIETMPHIM
FGGLTHEPAIELAKRLIKLTPPPLQKVFFTDSGSVAMEVAIKMAMQYWISLEQPQKNKLLALKNGYHGDTFATMSICDPD
NGMHRLFNSTLPQHFFAPAPQKGFDIDSDNQDIAEFETLLEKHHAEIAAVTLEPIVQGAGGMRFYRPDYLRQVRELCDEF
NVLLIADEIATGFGRTGKLFACEWANISPDIMALGKTLTGGNITLAATLATEKISDTISQGNPGLLMHGPTFMANPLACR
AASANIDVLLNSPWQDNIQRIESHFLNELMPLNALPGVDDTRVLGAIGVIELERNDLGETIQNLAVQNGVWIRPFGKLIY
TMPAYNIPTNQLTQLTEGLKTAIVQALQH

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=427, Percent_Identity=30.6791569086651, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI284507298, Length=321, Percent_Identity=32.398753894081, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI13994255, Length=365, Percent_Identity=26.027397260274, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI37574042, Length=400, Percent_Identity=24.75, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI226442709, Length=348, Percent_Identity=25.5747126436782, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI226442705, Length=400, Percent_Identity=24.25, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI24119277, Length=369, Percent_Identity=23.5772357723577, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI1786991, Length=417, Percent_Identity=55.1558752997602, Blast_Score=477, Evalue=1e-136,
Organism=Escherichia coli, GI145693181, Length=384, Percent_Identity=33.3333333333333, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1789759, Length=343, Percent_Identity=32.6530612244898, Blast_Score=158, Evalue=8e-40,
Organism=Escherichia coli, GI1788044, Length=388, Percent_Identity=30.6701030927835, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1789016, Length=379, Percent_Identity=29.023746701847, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1787560, Length=402, Percent_Identity=27.8606965174129, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1786349, Length=296, Percent_Identity=28.7162162162162, Blast_Score=105, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI25144271, Length=352, Percent_Identity=28.6931818181818, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32564660, Length=370, Percent_Identity=24.8648648648649, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI25144274, Length=146, Percent_Identity=37.6712328767123, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17541228, Length=440, Percent_Identity=20.6818181818182, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324386, Length=454, Percent_Identity=40.9691629955947, Blast_Score=357, Evalue=2e-99,
Organism=Saccharomyces cerevisiae, GI6323470, Length=377, Percent_Identity=28.9124668435013, Blast_Score=129, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6324432, Length=318, Percent_Identity=26.7295597484277, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321456, Length=320, Percent_Identity=26.5625, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21357415, Length=419, Percent_Identity=29.1169451073986, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI28574759, Length=366, Percent_Identity=27.8688524590164, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161085790, Length=349, Percent_Identity=28.6532951289398, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR007197 [H]

Pfam domain/function: PF00202 Aminotran_3; PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 47558; Mature: 47427

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHWQALLQFDQEHIWHPYAKMPSDTPAIGVAKTQGSIITLSDGNELVDGMSSWWAALHG
CCHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHC
YNHPVIQKAMIDQIETMPHIMFGGLTHEPAIELAKRLIKLTPPPLQKVFFTDSGSVAMEV
CCCHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHCCCCCHHEEEEECCCCHHHHH
AIKMAMQYWISLEQPQKNKLLALKNGYHGDTFATMSICDPDNGMHRLFNSTLPQHFFAPA
HHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCHHHHHHHHCCCHHHCCCC
PQKGFDIDSDNQDIAEFETLLEKHHAEIAAVTLEPIVQGAGGMRFYRPDYLRQVRELCDE
CCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEHHHHHCCCCCCCCCCCHHHHHHHHHHHH
FNVLLIADEIATGFGRTGKLFACEWANISPDIMALGKTLTGGNITLAATLATEKISDTIS
CCEEEEEHHHHHCCCCCCCEEEEECCCCCHHHHHHCCEECCCCEEEEEEHHHHHHHHHHH
QGNPGLLMHGPTFMANPLACRAASANIDVLLNSPWQDNIQRIESHFLNELMPLNALPGVD
CCCCCEEEECCCHHCCCHHHEECCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCC
DTRVLGAIGVIELERNDLGETIQNLAVQNGVWIRPFGKLIYTMPAYNIPTNQLTQLTEGL
CHHHHHEEEEEEECCHHHHHHHHHHHHHCCEEEEEHHHHHEECCCCCCCHHHHHHHHHHH
KTAIVQALQH
HHHHHHHHCC
>Mature Secondary Structure 
THWQALLQFDQEHIWHPYAKMPSDTPAIGVAKTQGSIITLSDGNELVDGMSSWWAALHG
CHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHC
YNHPVIQKAMIDQIETMPHIMFGGLTHEPAIELAKRLIKLTPPPLQKVFFTDSGSVAMEV
CCCHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHCCCCCHHEEEEECCCCHHHHH
AIKMAMQYWISLEQPQKNKLLALKNGYHGDTFATMSICDPDNGMHRLFNSTLPQHFFAPA
HHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCHHHHHHHHCCCHHHCCCC
PQKGFDIDSDNQDIAEFETLLEKHHAEIAAVTLEPIVQGAGGMRFYRPDYLRQVRELCDE
CCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEHHHHHCCCCCCCCCCCHHHHHHHHHHHH
FNVLLIADEIATGFGRTGKLFACEWANISPDIMALGKTLTGGNITLAATLATEKISDTIS
CCEEEEEHHHHHCCCCCCCEEEEECCCCCHHHHHHCCEECCCCEEEEEEHHHHHHHHHHH
QGNPGLLMHGPTFMANPLACRAASANIDVLLNSPWQDNIQRIESHFLNELMPLNALPGVD
CCCCCEEEECCCHHCCCHHHEECCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCC
DTRVLGAIGVIELERNDLGETIQNLAVQNGVWIRPFGKLIYTMPAYNIPTNQLTQLTEGL
CHHHHHEEEEEEECCHHHHHHHHHHHHHCCEEEEEHHHHHEECCCCCCCHHHHHHHHHHH
KTAIVQALQH
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA