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The map label for this gene is rep [H]

Identifier: 78484557

GI number: 78484557

Start: 251464

End: 253497

Strand: Reverse

Name: rep [H]

Synonym: Tcr_0212

Alternate gene names: 78484557

Gene position: 253497-251464 (Counterclockwise)

Preceding gene: 78484562

Following gene: 78484556

Centisome position: 10.44

GC content: 47.1

Gene sequence:

>2034_bases
ATGTTTGGACTGAATGATCGACAACTTAAAGGGGTGATGCACATTGAGACCCCTGCACTGGTTTTGGCCGGTGCCGGTTC
CGGCAAAACACGGGTTATTACCGAAAAAATCGCTTATTTGATTCGCAAACATGACGTGCAACCGCATCATATCTACGCCC
TGACCTTTACCAATAAAGCGGCCAAGGAAATGAAAGAGCGCGTCAGCAAACTTTTAAAAGACGATCCGAGCAAAGGTCTG
AATGTCTCGACCTTCCATAACTTGGGGCTGAACATTATCCGACAGGAATATCATGCGCTCGGGTATAAATCGACGTTTTC
GATTATGGATGCGACCGATACCAAACAAATTCTAAAAGAGCTGATGAAAAAACAACAGCTCAGTGAAGAAGAACTGGATG
GCGTGCAATGGGATATTTCCAATTGGAAAAACGCCCACATCAGTCCGGAAAAAGCGCTGGAAATGGCGGAAGACAACCTG
CAACAGGCACGCGCCATTTTATACGATTACTATCAAAAGCAGCTGCATGCTTACAACTCGGTCGACTTTGATGACTTAAT
TGGCCTGCCGGTGCGCTTGTTCAATGAAAACCCGCAAATATTAGACAAGTGGCAAAACAAAGTCCGTTACCTCTTGGTGG
ATGAGTATCAAGACACCAATGCTGCGCAATATGAGTTGGTAAAACAACTGGTGGGTGTACGCGCCAAATTCACCGTGGTT
GGGGATGACGATCAATCCATTTATGCTTGGCGCGGTGCTCAGCCAGAAAACCTAGAACTGCTGAAAAAAGACTTCCCGAA
TTTGGAAGTAATCAAGCTGGAACAAAACTACCGCTCCAGTAACCGTATTTTGCAAGCGGCAAACACCTTGATTGCCAACA
ACCCGCACGTGTTTGAAAAACGCCTCTGGTCGGAAATGGGCATGGGCGATATGTTGCGTGTGCTGGCCTGTTCAGACGAA
AATCATGAAGCCGAACGCGTGATATCTGAAATCATCGTTCACAAATTCAAACACCGCACCCAATTTAAAGACTATGCCAT
TCTGTATCGCGGTAACTTTCAGTCCCGTTTATTCGAACGCGCCTTGCGCGAACAAAACATTCCTTACAAGTTATCCGGCG
GTCAATCGTTCTTCGACAAAGCCGAAATTAAAGATGTGATGAGCTACCTTAAACTGATCGTTAACCCGGATGATGATGCG
GCCTTTTTACGCATAGTGAACACCCCTCGCCGTGAAATCGGTGCCTCCACTTTGGAAAAGCTGGCGACTTACGCCACCAA
ACGCGGCGTTAGCTTGTTTGACGCGACCCAAGAGCTGGGACTCAGCACGGTACTGAGTGAGAAAGCTCTGCAACGGGTGC
AGCACTTCGGCGGCCTGCTATTGTCTTGGGCACAAGAAGCCGATGCGTTAGCGGGCACGGAAGTCACATCCTTTGTGCGG
CAACTGATTGAAAAACTGGAATATGATAATTGGCTACACGAAACCAGTAACACCCCCCGTGCGGCGGAAAAACGCATTGA
AAACGTACGCGACTTATTGCTCTGGATTGAACGCATTATTGATAAAGCGCTGAATGAAGATGGCGACGAACTCCGTTTGG
AAAAAATTGTTGCTCATATGACGCTGATGGATTTAATGGAACGCAATGAAGAAGACTCCGAGCCGAACATGGTCACATTG
ATGACCTTGCATGCCTCCAAAGGGCTTGAATTTCCGCATGTATTCCTCATCGGCATGGAAGAAGAGCTGCTGCCACACAA
ACAAAACATGGAATCCCCTGGATTGGAAGAAGAGCGCCGTTTGGCTTATGTAGGAATTACCCGTGCCAAGCGCAGTCTGA
CGATGACGTATGCGAAAAAACGCAAGAAATACGGTGAAGAACTCAAATGTGAACCAAGCCGCTTTTTAGAAGAACTACCA
GAAGAACTTTTACAGTGGGAAGGCAAACCTGGCGTTGAGCTGTCTCCTGAAGAACAAAAAGTCACCGGAAATGCTCATTT
AGAAAACTTAAAAGCCATGCTCAACCCGGGTTAA

Upstream 100 bases:

>100_bases
AAAATAACGCAGATTGGGCAATTTCTCTAAATTCGAAACGCCGACATTCTGGTATCATATTGTATCTTTTTTATTACAGC
GCGCGAATGGATTTACACCG

Downstream 100 bases:

>100_bases
CCCTACTCGATTAACCAAAAAGGACGACAGATGATTGCAGACTTTCAAATCGCCAACCTCCCTAAAATTCAATTCGGCTG
GGGGATTCGAGATCAATTTG

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 677; Mature: 677

Protein sequence:

>677_residues
MFGLNDRQLKGVMHIETPALVLAGAGSGKTRVITEKIAYLIRKHDVQPHHIYALTFTNKAAKEMKERVSKLLKDDPSKGL
NVSTFHNLGLNIIRQEYHALGYKSTFSIMDATDTKQILKELMKKQQLSEEELDGVQWDISNWKNAHISPEKALEMAEDNL
QQARAILYDYYQKQLHAYNSVDFDDLIGLPVRLFNENPQILDKWQNKVRYLLVDEYQDTNAAQYELVKQLVGVRAKFTVV
GDDDQSIYAWRGAQPENLELLKKDFPNLEVIKLEQNYRSSNRILQAANTLIANNPHVFEKRLWSEMGMGDMLRVLACSDE
NHEAERVISEIIVHKFKHRTQFKDYAILYRGNFQSRLFERALREQNIPYKLSGGQSFFDKAEIKDVMSYLKLIVNPDDDA
AFLRIVNTPRREIGASTLEKLATYATKRGVSLFDATQELGLSTVLSEKALQRVQHFGGLLLSWAQEADALAGTEVTSFVR
QLIEKLEYDNWLHETSNTPRAAEKRIENVRDLLLWIERIIDKALNEDGDELRLEKIVAHMTLMDLMERNEEDSEPNMVTL
MTLHASKGLEFPHVFLIGMEEELLPHKQNMESPGLEEERRLAYVGITRAKRSLTMTYAKKRKKYGEELKCEPSRFLEELP
EELLQWEGKPGVELSPEEQKVTGNAHLENLKAMLNPG

Sequences:

>Translated_677_residues
MFGLNDRQLKGVMHIETPALVLAGAGSGKTRVITEKIAYLIRKHDVQPHHIYALTFTNKAAKEMKERVSKLLKDDPSKGL
NVSTFHNLGLNIIRQEYHALGYKSTFSIMDATDTKQILKELMKKQQLSEEELDGVQWDISNWKNAHISPEKALEMAEDNL
QQARAILYDYYQKQLHAYNSVDFDDLIGLPVRLFNENPQILDKWQNKVRYLLVDEYQDTNAAQYELVKQLVGVRAKFTVV
GDDDQSIYAWRGAQPENLELLKKDFPNLEVIKLEQNYRSSNRILQAANTLIANNPHVFEKRLWSEMGMGDMLRVLACSDE
NHEAERVISEIIVHKFKHRTQFKDYAILYRGNFQSRLFERALREQNIPYKLSGGQSFFDKAEIKDVMSYLKLIVNPDDDA
AFLRIVNTPRREIGASTLEKLATYATKRGVSLFDATQELGLSTVLSEKALQRVQHFGGLLLSWAQEADALAGTEVTSFVR
QLIEKLEYDNWLHETSNTPRAAEKRIENVRDLLLWIERIIDKALNEDGDELRLEKIVAHMTLMDLMERNEEDSEPNMVTL
MTLHASKGLEFPHVFLIGMEEELLPHKQNMESPGLEEERRLAYVGITRAKRSLTMTYAKKRKKYGEELKCEPSRFLEELP
EELLQWEGKPGVELSPEEQKVTGNAHLENLKAMLNPG
>Mature_677_residues
MFGLNDRQLKGVMHIETPALVLAGAGSGKTRVITEKIAYLIRKHDVQPHHIYALTFTNKAAKEMKERVSKLLKDDPSKGL
NVSTFHNLGLNIIRQEYHALGYKSTFSIMDATDTKQILKELMKKQQLSEEELDGVQWDISNWKNAHISPEKALEMAEDNL
QQARAILYDYYQKQLHAYNSVDFDDLIGLPVRLFNENPQILDKWQNKVRYLLVDEYQDTNAAQYELVKQLVGVRAKFTVV
GDDDQSIYAWRGAQPENLELLKKDFPNLEVIKLEQNYRSSNRILQAANTLIANNPHVFEKRLWSEMGMGDMLRVLACSDE
NHEAERVISEIIVHKFKHRTQFKDYAILYRGNFQSRLFERALREQNIPYKLSGGQSFFDKAEIKDVMSYLKLIVNPDDDA
AFLRIVNTPRREIGASTLEKLATYATKRGVSLFDATQELGLSTVLSEKALQRVQHFGGLLLSWAQEADALAGTEVTSFVR
QLIEKLEYDNWLHETSNTPRAAEKRIENVRDLLLWIERIIDKALNEDGDELRLEKIVAHMTLMDLMERNEEDSEPNMVTL
MTLHASKGLEFPHVFLIGMEEELLPHKQNMESPGLEEERRLAYVGITRAKRSLTMTYAKKRKKYGEELKCEPSRFLEELP
EELLQWEGKPGVELSPEEQKVTGNAHLENLKAMLNPG

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=674, Percent_Identity=54.4510385756677, Blast_Score=705, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=646, Percent_Identity=39.1640866873065, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1787196, Length=343, Percent_Identity=28.5714285714286, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=484, Percent_Identity=32.2314049586777, Blast_Score=194, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6324477, Length=406, Percent_Identity=23.8916256157635, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77916; Mature: 77916

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGLNDRQLKGVMHIETPALVLAGAGSGKTRVITEKIAYLIRKHDVQPHHIYALTFTNKA
CCCCCCHHHCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCHH
AKEMKERVSKLLKDDPSKGLNVSTFHNLGLNIIRQEYHALGYKSTFSIMDATDTKQILKE
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHH
LMKKQQLSEEELDGVQWDISNWKNAHISPEKALEMAEDNLQQARAILYDYYQKQLHAYNS
HHHHHCCCHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VDFDDLIGLPVRLFNENPQILDKWQNKVRYLLVDEYQDTNAAQYELVKQLVGVRAKFTVV
CCHHHHHCCCCEEECCCHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEE
GDDDQSIYAWRGAQPENLELLKKDFPNLEVIKLEQNYRSSNRILQAANTLIANNPHVFEK
CCCCCCEEEECCCCCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHH
RLWSEMGMGDMLRVLACSDENHEAERVISEIIVHKFKHRTQFKDYAILYRGNFQSRLFER
HHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHH
ALREQNIPYKLSGGQSFFDKAEIKDVMSYLKLIVNPDDDAAFLRIVNTPRREIGASTLEK
HHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHH
LATYATKRGVSLFDATQELGLSTVLSEKALQRVQHFGGLLLSWAQEADALAGTEVTSFVR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
QLIEKLEYDNWLHETSNTPRAAEKRIENVRDLLLWIERIIDKALNEDGDELRLEKIVAHM
HHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
TLMDLMERNEEDSEPNMVTLMTLHASKGLEFPHVFLIGMEEELLPHKQNMESPGLEEERR
HHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCHHHH
LAYVGITRAKRSLTMTYAKKRKKYGEELKCEPSRFLEELPEELLQWEGKPGVELSPEEQK
EEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
VTGNAHLENLKAMLNPG
CCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MFGLNDRQLKGVMHIETPALVLAGAGSGKTRVITEKIAYLIRKHDVQPHHIYALTFTNKA
CCCCCCHHHCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCHH
AKEMKERVSKLLKDDPSKGLNVSTFHNLGLNIIRQEYHALGYKSTFSIMDATDTKQILKE
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHH
LMKKQQLSEEELDGVQWDISNWKNAHISPEKALEMAEDNLQQARAILYDYYQKQLHAYNS
HHHHHCCCHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VDFDDLIGLPVRLFNENPQILDKWQNKVRYLLVDEYQDTNAAQYELVKQLVGVRAKFTVV
CCHHHHHCCCCEEECCCHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEE
GDDDQSIYAWRGAQPENLELLKKDFPNLEVIKLEQNYRSSNRILQAANTLIANNPHVFEK
CCCCCCEEEECCCCCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHH
RLWSEMGMGDMLRVLACSDENHEAERVISEIIVHKFKHRTQFKDYAILYRGNFQSRLFER
HHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHH
ALREQNIPYKLSGGQSFFDKAEIKDVMSYLKLIVNPDDDAAFLRIVNTPRREIGASTLEK
HHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHH
LATYATKRGVSLFDATQELGLSTVLSEKALQRVQHFGGLLLSWAQEADALAGTEVTSFVR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
QLIEKLEYDNWLHETSNTPRAAEKRIENVRDLLLWIERIIDKALNEDGDELRLEKIVAHM
HHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
TLMDLMERNEEDSEPNMVTLMTLHASKGLEFPHVFLIGMEEELLPHKQNMESPGLEEERR
HHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCHHHH
LAYVGITRAKRSLTMTYAKKRKKYGEELKCEPSRFLEELPEELLQWEGKPGVELSPEEQK
EEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
VTGNAHLENLKAMLNPG
CCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]