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The map label for this gene is dosC [H]

Identifier: 78358791

GI number: 78358791

Start: 3703965

End: 3705275

Strand: Direct

Name: dosC [H]

Synonym: Dde_3752

Alternate gene names: 78358791

Gene position: 3703965-3705275 (Clockwise)

Preceding gene: 78358790

Following gene: 78358793

Centisome position: 99.3

GC content: 51.11

Gene sequence:

>1311_bases
ATGGCAGCACCTTTCCGGGCCGTCTGCCACGGAGCAGAGGACGCCTTGTCTTGTCAGGCGCGCAAATACCGGTATAGTGC
CTCCACACAACGCACATTACAGCACCATATCATGATAGACATCCGCACAGTTCTCATACTCAATGCTCTTGTATTTCTTA
TCGCCACGGGCTTTTCGTATTATATGTATACAAGAAAAGTTCCTGTAGATGGTGTAAAATGGCTTATTGCCGGATTCGGT
TGCGGACTTGTAAGCATGGTAAGCTTTTTTACAAGAGGCTATATACCAACAGTCTACTCAGTACTTTTCGGCAACTCACT
TGCACTGCTTTTTCTTATTATAATATATAACTGGGTAAGAGTATTTCTTGGCAAAGAAAAACTGACAAAACGCCAGTGGT
CTTTTATCGCCGCTATCATAGCTGCATCAGCCTGCACCTATACATACTATGTTGCCATCGACAATAATATTTTCATACGG
ATATTCATTGTCAATACTCTTGTCGGCATAATAGGACTTCTCTGCGGATACACGCTTGTTCGCGTAAAAGACGTCACATT
CTGCAAAATAACAGGCATCAGCTGCCTGCTGCACGGCTCATACAGCATTATTTACGGAACAGTCAGCCTGCTAAGCCAGC
AGAGTTCTGTGGAGTATCTGCAGGCCGGCCCGCTTATCAAATTTTCCGTTCTTGTCTCTCTGGTTTTCGGCATCATGCTG
ATGATGGGAGTCAGCAGCATGATCAATGAACGGCTGGTACAGATGCTGGAAAAGGAAGCCCGGACAGACCCGCTGACCGG
ACTGGGCAACCGGCGGGCTCTATACACGGCCGCAGCCCATCATGTGGCCCGCCTGCACCGCCTGTGTGAACCGCTGGTCA
TACTTGTGATGGACCTTGACCGGTTCAAATCCGTCAATGACAAGTTCGGCCATCCTGCAGGTGACGCCCTGCTCCGGCAT
TTTGCCGCCATACTGCAAAAACAGTTCAGAGGCACAGATCAACTGTTCCGCTTCGGCGGAGAAGAGTTTGTCGCGCTGCT
GCAGCCTTCGGACGAACGGGGTGCTGTATGTCTGGCCGAAAGACTCCGCAGGGAGGTTGCCGCATGCCCCCTTCAGCTGG
GCGGCGCTTCCATCGCTGTCACCGTCAGTATAGGCATTGCCAATATGCTGCCGGAAGAACCCGACATCACCAGAGCACTC
GGCCGTGCCGATCAGGCTTTGTATAAAGCCAAGGCAGGCGGAAGAAACAGGGTGGAAAGTTCGTACACCGTGCATGCCGC
CGACTGTACAGAGCCTGTACCCGCCGTCTGA

Upstream 100 bases:

>100_bases
CGGAGACATAAAACAATCACGCAGGAAGACGCCTCCGGCGGACAGATTATAAGTTTAATTTTTTCATCGCCCGATATGGT
AAACATATCCGCCTGTCCGT

Downstream 100 bases:

>100_bases
CTTTCACCGCACCGGCGCGCTTGCCGGACTTCCTTCACTCCTTTCCTTCTCCGGTGTCCGCGTGCCGTTGCGTGCGTTAT
CACGTTCCGCCCGCGCCTGC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MAAPFRAVCHGAEDALSCQARKYRYSASTQRTLQHHIMIDIRTVLILNALVFLIATGFSYYMYTRKVPVDGVKWLIAGFG
CGLVSMVSFFTRGYIPTVYSVLFGNSLALLFLIIIYNWVRVFLGKEKLTKRQWSFIAAIIAASACTYTYYVAIDNNIFIR
IFIVNTLVGIIGLLCGYTLVRVKDVTFCKITGISCLLHGSYSIIYGTVSLLSQQSSVEYLQAGPLIKFSVLVSLVFGIML
MMGVSSMINERLVQMLEKEARTDPLTGLGNRRALYTAAAHHVARLHRLCEPLVILVMDLDRFKSVNDKFGHPAGDALLRH
FAAILQKQFRGTDQLFRFGGEEFVALLQPSDERGAVCLAERLRREVAACPLQLGGASIAVTVSIGIANMLPEEPDITRAL
GRADQALYKAKAGGRNRVESSYTVHAADCTEPVPAV

Sequences:

>Translated_436_residues
MAAPFRAVCHGAEDALSCQARKYRYSASTQRTLQHHIMIDIRTVLILNALVFLIATGFSYYMYTRKVPVDGVKWLIAGFG
CGLVSMVSFFTRGYIPTVYSVLFGNSLALLFLIIIYNWVRVFLGKEKLTKRQWSFIAAIIAASACTYTYYVAIDNNIFIR
IFIVNTLVGIIGLLCGYTLVRVKDVTFCKITGISCLLHGSYSIIYGTVSLLSQQSSVEYLQAGPLIKFSVLVSLVFGIML
MMGVSSMINERLVQMLEKEARTDPLTGLGNRRALYTAAAHHVARLHRLCEPLVILVMDLDRFKSVNDKFGHPAGDALLRH
FAAILQKQFRGTDQLFRFGGEEFVALLQPSDERGAVCLAERLRREVAACPLQLGGASIAVTVSIGIANMLPEEPDITRAL
GRADQALYKAKAGGRNRVESSYTVHAADCTEPVPAV
>Mature_435_residues
AAPFRAVCHGAEDALSCQARKYRYSASTQRTLQHHIMIDIRTVLILNALVFLIATGFSYYMYTRKVPVDGVKWLIAGFGC
GLVSMVSFFTRGYIPTVYSVLFGNSLALLFLIIIYNWVRVFLGKEKLTKRQWSFIAAIIAASACTYTYYVAIDNNIFIRI
FIVNTLVGIIGLLCGYTLVRVKDVTFCKITGISCLLHGSYSIIYGTVSLLSQQSSVEYLQAGPLIKFSVLVSLVFGIMLM
MGVSSMINERLVQMLEKEARTDPLTGLGNRRALYTAAAHHVARLHRLCEPLVILVMDLDRFKSVNDKFGHPAGDALLRHF
AAILQKQFRGTDQLFRFGGEEFVALLQPSDERGAVCLAERLRREVAACPLQLGGASIAVTVSIGIANMLPEEPDITRALG
RADQALYKAKAGGRNRVESSYTVHAADCTEPVPAV

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=175, Percent_Identity=38.8571428571429, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI87081881, Length=190, Percent_Identity=38.9473684210526, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787816, Length=179, Percent_Identity=32.9608938547486, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=37.2670807453416, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786584, Length=227, Percent_Identity=29.9559471365639, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082007, Length=178, Percent_Identity=35.9550561797753, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787802, Length=238, Percent_Identity=28.1512605042017, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788381, Length=181, Percent_Identity=32.5966850828729, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1787541, Length=180, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1788085, Length=172, Percent_Identity=30.2325581395349, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081977, Length=170, Percent_Identity=30, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87081974, Length=190, Percent_Identity=27.8947368421053, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787056, Length=124, Percent_Identity=34.6774193548387, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 48159; Mature: 48028

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPFRAVCHGAEDALSCQARKYRYSASTQRTLQHHIMIDIRTVLILNALVFLIATGFSY
CCCCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
YMYTRKVPVDGVKWLIAGFGCGLVSMVSFFTRGYIPTVYSVLFGNSLALLFLIIIYNWVR
EEEEECCCCCHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
VFLGKEKLTKRQWSFIAAIIAASACTYTYYVAIDNNIFIRIFIVNTLVGIIGLLCGYTLV
HHHCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHH
RVKDVTFCKITGISCLLHGSYSIIYGTVSLLSQQSSVEYLQAGPLIKFSVLVSLVFGIML
HCCCCEEEEEECEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
MMGVSSMINERLVQMLEKEARTDPLTGLGNRRALYTAAAHHVARLHRLCEPLVILVMDLD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFKSVNDKFGHPAGDALLRHFAAILQKQFRGTDQLFRFGGEEFVALLQPSDERGAVCLAE
HHHCCCHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHEEEECCCCCCCHHHHHH
RLRREVAACPLQLGGASIAVTVSIGIANMLPEEPDITRALGRADQALYKAKAGGRNRVES
HHHHHHHHCCCEECCCEEEEEEEECHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
SYTVHAADCTEPVPAV
CCEEEECCCCCCCCCC
>Mature Secondary Structure 
AAPFRAVCHGAEDALSCQARKYRYSASTQRTLQHHIMIDIRTVLILNALVFLIATGFSY
CCCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
YMYTRKVPVDGVKWLIAGFGCGLVSMVSFFTRGYIPTVYSVLFGNSLALLFLIIIYNWVR
EEEEECCCCCHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
VFLGKEKLTKRQWSFIAAIIAASACTYTYYVAIDNNIFIRIFIVNTLVGIIGLLCGYTLV
HHHCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHH
RVKDVTFCKITGISCLLHGSYSIIYGTVSLLSQQSSVEYLQAGPLIKFSVLVSLVFGIML
HCCCCEEEEEECEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
MMGVSSMINERLVQMLEKEARTDPLTGLGNRRALYTAAAHHVARLHRLCEPLVILVMDLD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFKSVNDKFGHPAGDALLRHFAAILQKQFRGTDQLFRFGGEEFVALLQPSDERGAVCLAE
HHHCCCHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHEEEECCCCCCCHHHHHH
RLRREVAACPLQLGGASIAVTVSIGIANMLPEEPDITRALGRADQALYKAKAGGRNRVES
HHHHHHHHCCCEECCCEEEEEEEECHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
SYTVHAADCTEPVPAV
CCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]