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The map label for this gene is nisX1 [H]

Identifier: 78358786

GI number: 78358786

Start: 3700066

End: 3700425

Strand: Reverse

Name: nisX1 [H]

Synonym: Dde_3747

Alternate gene names: 78358786

Gene position: 3700425-3700066 (Counterclockwise)

Preceding gene: 78358787

Following gene: 78358784

Centisome position: 99.2

GC content: 53.33

Gene sequence:

>360_bases
TTGGAGGCCGCTATCCAACTGAGAAGACCTGGGCTCGGACTGCTGCATCATAGCGACCAGGGGTGCCAATACACCAGTGG
AGCCTTCTCCGCAGTGCTTGATCAGTACGGAATCGTATGCAGTATGAGTCGCCGGGGGAACTGCTGGGACAATGCAGTCA
CGGAAAGTTTCTTTAGCAAGCTCAAGCGGGAGTGGGTGCGCGGGAAACGGTATCGGACGCGCAACGACGCACGACAGGAC
ATCTTCCTTTATCTGGAAGCGTTTTATAATCGGAAAAGGCGACATGCCTTTCTTGGCTACCAAAGTCCGGAGGCATTCGA
ACAATTGTTTTATCAGGCCAAGCAAAAACAGGCGGCGTAA

Upstream 100 bases:

>100_bases
AGGCTGGCTGTACCTTGCTGTCGTCTTGGATCTGTTCTCAAGACGTGTGGTGGGTTGGGCCATGTCTGAATGAGCAGATG
CCCACCTGGCTTGCCAAGCG

Downstream 100 bases:

>100_bases
TCTCTCGGCCACTGTCCGTTTTTCTTATGGCATTTCACCCTGCCCGCCGGAGGCGTTTTCCTGTGTGATTGTTTTCTGTC
TCCGACGGGCAAGGGGACTG

Product: ISRSO8-transposase orfB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 119; Mature: 119

Protein sequence:

>119_residues
MEAAIQLRRPGLGLLHHSDQGCQYTSGAFSAVLDQYGIVCSMSRRGNCWDNAVTESFFSKLKREWVRGKRYRTRNDARQD
IFLYLEAFYNRKRRHAFLGYQSPEAFEQLFYQAKQKQAA

Sequences:

>Translated_119_residues
MEAAIQLRRPGLGLLHHSDQGCQYTSGAFSAVLDQYGIVCSMSRRGNCWDNAVTESFFSKLKREWVRGKRYRTRNDARQD
IFLYLEAFYNRKRRHAFLGYQSPEAFEQLFYQAKQKQAA
>Mature_119_residues
MEAAIQLRRPGLGLLHHSDQGCQYTSGAFSAVLDQYGIVCSMSRRGNCWDNAVTESFFSKLKREWVRGKRYRTRNDARQD
IFLYLEAFYNRKRRHAFLGYQSPEAFEQLFYQAKQKQAA

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1788405, Length=107, Percent_Identity=40.1869158878505, Blast_Score=85, Evalue=2e-18,
Organism=Escherichia coli, GI1787263, Length=107, Percent_Identity=40.1869158878505, Blast_Score=85, Evalue=2e-18,
Organism=Escherichia coli, GI1786753, Length=107, Percent_Identity=40.1869158878505, Blast_Score=85, Evalue=2e-18,
Organism=Escherichia coli, GI1786570, Length=107, Percent_Identity=40.1869158878505, Blast_Score=85, Evalue=2e-18,
Organism=Escherichia coli, GI1786490, Length=107, Percent_Identity=40.1869158878505, Blast_Score=85, Evalue=2e-18,
Organism=Escherichia coli, GI1789981, Length=77, Percent_Identity=41.5584415584416, Blast_Score=62, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 13952; Mature: 13952

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAAIQLRRPGLGLLHHSDQGCQYTSGAFSAVLDQYGIVCSMSRRGNCWDNAVTESFFSK
CCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHH
LKREWVRGKRYRTRNDARQDIFLYLEAFYNRKRRHAFLGYQSPEAFEQLFYQAKQKQAA
HHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEAAIQLRRPGLGLLHHSDQGCQYTSGAFSAVLDQYGIVCSMSRRGNCWDNAVTESFFSK
CCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHH
LKREWVRGKRYRTRNDARQDIFLYLEAFYNRKRRHAFLGYQSPEAFEQLFYQAKQKQAA
HHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1482189; 11337471; 1482192 [H]